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Search Results: 1 - 10 of 6939 matches for " Rosane Christine Hahn "
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Aspectos micológicos e suscetibilidade in vitro de leveduras do gênero Candida em pacientes HIV-positivos provenientes do Estado de Mato Grosso
Favalessa, Olivia Cometti;Martins, Marilena dos Anjos;Hahn, Rosane Christine;
Revista da Sociedade Brasileira de Medicina Tropical , 2010, DOI: 10.1590/S0037-86822010000600014
Abstract: introduction: candidiasis is one of the most common fungal infections among patients infected by human immunodeficiency virus. the present study aimed to characterize yeasts of the genus candida from distinct clinical samples from hiv-positive patients and determine the in vitro susceptibility profile to five antifungal drugs. methods: characterization of candida sp was achieved using the classic methodology: biochemical (zymogram and auxanogram) and micromorphology (germinative tube growth test and slide microculture) tests. genotypic technique (pcr) and identification by the commercial method api 20c aux (biomeriéux) were also performed. to determine the in vitro susceptibility profile, five antifungal drugs were used (ketoconazole, fluconazole, itraconazole, voriconazole and amphotericin-b) following a commercially available method, the etest. results: the procedure isolated 105 yeasts of the genus candida from 102 hiv-infected patients. of these, 82 (78.1%) were characterized as candida albicans, 8 (7.6%) as c. parapsilosi s, 8 (7.6%) c. tropicalis, 4 (3.8%) c. krusei, 2 (1.9%) c. glabrata, and 1 (1%) as c. guiilliermondii. conclusions: considering the general profile of sensitivity, 60% of isolates were susceptible to all the antifungal drugs tested; however, the species c. tropicalis and c. krusei showed a tendency toward higher mics to azoles than those obtained for c. albicans, suggesting resistance.
Acute disseminated paracoccidioidomycosis in a 3 year-old child
Grossklaus, Daciene de Arruda;Tadano, Tomoko;Breder, Sandra Assis;Hahn, Rosane Christine;
Brazilian Journal of Infectious Diseases , 2009, DOI: 10.1590/S1413-86702009000300018
Abstract: clinical cases involving paracoccidioidomycosis in children, diagnosed in mato grosso state, in the central western region of brazil, are rare despite the state being classified with a moderate to high incidence. we describe a clinical case of infant acute disseminated paracoccidioidomycosis in mato grosso state, brazil, highlighting the importance of early differential diagnosis from other severe pathologies, thus contributing to the survival of compromised patients. a 3 year-old male child, weighing 12.8 kg, originating from vila rica, mt, brazil. the patient presented intermittent 40oc fever evolving over 40 days, dry cough and painless bilateral cervical adenomegaly, showing no signs of inflammation. this was associated with diarrhea, distension and important abdominal pain and weight loss. diagnosis was achieved by visualization of paracoccidioides brasiliensis yeasts in a direct mycological exam and posterior fungus isolation in culture medium. the patient evolved presenting good clinical response to antifungal treatment and progressive reduction of abdominal and cervical ganglions. to improve the prognosis of compromised patients it is essential that professionals realize a full clinical-laboratorial evaluation, including differential diagnoses for other severe pathologies, as early as possible. the degree and intensity of paracoccidioidomycosis compromise are determining factors for defining the most efficient treatment.
Micoses sistêmicas: fatores associados ao óbito em pacientes com infec??o pelo vírus da imunodeficiência humana, Cuiabá, Estado de Mato Grosso, 2005-2008
Ribeiro, Luciano Correa;Hahn, Rosane Christine;Favalessa, Olivia Cometi;Tadano, Tomoko;Fontes, Cor Jesus Fernandes;
Revista da Sociedade Brasileira de Medicina Tropical , 2009, DOI: 10.1590/S0037-86822009000600017
Abstract: between 2005 and 2008, the prevalence of systemic mycosis among 1,300 hiv/aids patients in cuiabá, mato grosso, was 4.6%. the fungus species isolated were cryptococcus neoformans in 50%, cryptococcus gattii in 1.6%, cryptococcus spp in 6.6%, histoplasma capsulatum in 38.3% and paracoccidioides brasiliensis in 3.3%. death was recorded in the cases of 32 patients (53.3%), and cryptococcosis was the main cause. the cd4+ t lymphocyte count was low and similar among patients who survived or died due to systemic mycosis. the factors independently associated with the deaths of these patients were alcoholism (or: 8.2; 95% ci: 1.4-62.1; p = 0005) and the mean level of lactate dehydrogenase [758 (182) u/l vs. 416 (268) u/l; p < 0001]. the findings showed that systemic mycosis was highly lethal among the patients with hiv/aids in cuiabá and suggested that clinical-laboratory characteristics such as alcoholism and early elevation of lactate dehydrogenase may be factors relating to worse prognosis under these conditions.
Indoor Air Mycological Survey and Occupational Exposure in Libraries in Mato Grosso-Central Region—Brazil  [PDF]
Diniz Pereira Leite Júnior, Ronaldo Sousa Pereira, Washington Santos de Almeida, Sara de Almeida Alves Sim?es, Ana Caroline Akeme Yamamoto, Janaina Vasconcellos Ribeiro de Souza, Evelin Rodrigues Martins, Fábio Alexandre Leal dos Santos, Rosane Christine Hahn
Advances in Microbiology (AiM) , 2018, DOI: 10.4236/aim.2018.84022
Abstract: Background: Indoor air quality in environments where there is great circulation of people, posing risks to the health of its occupants, including allergic problems, infections and contaminations, can be aided by climatic factors, chemicals and biological agents housed in these environments, influencing the location and providing favorable conditions for the degradation of bibliographic collections. The present study investigated the presence of fungi in indoor environments in seven public and private libraries in the central region of Brazil, Mato Grosso, and verified the impact on occupational health. Results: A total of 26,194 fungal specimens were isolated from 342 dust samples collected using three techniques: Andersen’s sampler (12.3%), exposure plate dish (25.1%) and sterile swab (62.6%). A total of 184 fungal species were identified: 156 (84.8%) mycelial fungi and 28 (15.2%) yeast fungi, belonging 54 fungal genera, 43 (79.6%) mycelial fungi and 11 (20.4%) yeast fungi. The genus Aspergillus (40.6%) was one of the main fungi present in indoor air. Aspergillus niger (12.3%) was identified as the most prevalent species in literary environments, followed by Cryptococcus spp. (7.1%) and Cladosporium cladosporioides (7.0%). In relation to seasonal distribution, there was a greater fungal isolation in the dry season (54%); followed by the rainy season (46%). Conclusion: These results suggest the substrates researched in the evaluated environments presented in the form of documents, books and papers associated with dust and air humidity become suitable for microbiological proliferation. These findings highlight the importance of minimizing the risk of exposure to fungal agents, identified in pathogenic and toxigenic microenvironments in library collections.
Leveduras do gênero Candida isoladas de sítios anatomicamente distintos de profissionais militares em Cuiabá (MT), Brasil
Leite Júnior, Diniz Pereira;Yamamoto, Ana Caroline Akeme;Martins, Evelin Rodrigues;Teixeira, Acy Fátima Rodrigues;Hahn, Rosane Christine;
Anais Brasileiros de Dermatologia , 2011, DOI: 10.1590/S0365-05962011000400008
Abstract: backgrounds: some fungi are natural inhabitants of the human body but may result in disease when conditions are conducive to their development. yeast infections are common and often occur in the skin and mucous membranes; however emerging species have changed this epidemiological profile. the ability to colonize different anatomical sites has been associated with the pathogenicity of candida when environmental conditions are particularly favorable. in the case of hot, humid climates, the attrition suffered by the skin and weakened immune defenses may result in yeasts becoming pathogenic rather than commensal organisms. objective: the objective of this study was to diagnose yeast infections in military personnel and to evaluate the frequency of these infections in the individuals evaluated. methods: the clinical material analyzed was seeded in duplicate in sabouraud dextrose agar (difco?) and mycosel medium (difco?). the etiological agents were identified by observing the germ tubes, microculture and physiological characteristics, assimilation of carbon sources (auxanogram) and fermentation of carbon sources (zymogram). results: of a total of 197 patients evaluated, 91 (46.2%) had episodes of candidiasis. the genitocrural region was the most commonly affected area (47.7%) followed by the interdigital regions (between the toes or fingers) (27.8%). candida albicans, candida parapsilosis, candida tropicalis, candida glabrata and emergent species such as candida krusei and candida guilliermondii were found. conclusions: in the work environment, having to use shoes and uniforms for extended periods of time, in addition to stress and perspiration, were considered predisposing factors for the development of fungal infections.
Vulvovaginal candidiasis in Mato Grosso, Brazil: pregnancy status, causative species and drugs tests
Dias, Luciana Basili;Melhem, Márcia de Souza Carvalho;Szeszs, Maria Walderez;Meirelles Filho, José;Hahn, Rosane Christine;
Brazilian Journal of Microbiology , 2011, DOI: 10.1590/S1517-83822011000400009
Abstract: causative agent in majority of vvc is candida albicans, but infection due to non-c. albicans is common. use of empiric antifungal therapy in brazil due to syndromic management of vulvovaginitis could act as risk factor for increase resistance among vvc causative agents. from mato grosso patients, 160 with culture-proved among 404 women who had clinical symptoms of vvc, were enrolled in this study. 70 non-pregnant women and 90 pregnant women were included. candida albicans was the most prevalent, representing 72.9% in the non-pregnant group and 92.3% in the pregnant group. differences in species distribution were noted between the two groups, being c. parapsilosis the second more prevalent species among non-pregnant women. susceptibility testing revealed high susceptibility to fluconazole (except for c. krusei), itraconazole, ketoconazole, and amphotericin b regardless the species (c. albicans, c. parapsilosis, c. tropicalis, c. glabrata, c. krusei) analyzed.
Fungal agents in different anatomical sites in Public Health Services in Cuiabá, state of Mato Grosso, Brazil
Araújo, Sebasti?o Martins de;Fontes, Cor Jésus Fernandes;Leite Júnior, Diniz Pereira;Hahn, Rosane Christine;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2012, DOI: 10.1590/S0036-46652012000100002
Abstract: introduction: a contribution to the regional epidemiological profile of the most common fungal agents in public health services in cuiabá, state of mato grosso, including university hospitals and polyclinics. methods: clinical specimens (n = 1,496) from 1,078 patients were collected, submitted to direct mycological exam (potash or stick tape method) and cultured in specific mediums. dermatophytic and non-dermatophytic agents were identified according to micromorphology (ridell technique). results: the majority of the 1,496 specimens were skin (n = 985) and nail exams (n = 472). of the 800 positive cultures, 246 (30.8%) corresponded to dermatophytes and 336 (42%) to yeasts of the genus candida, 190 (23.7%) to other yeasts, 27 (3.4%) to non-dermatophytic filamentous fungi and one (0.1%) the agent of subcutaneous mycosis. lesions considered primary occurred in greater numbers (59.5%) than recurrent lesions (37.4%), with a greater concentration of positivity occurring on the arms and legs. conclusions: comorbidities, allergies and diabetes mellitus were conditions associated with greater positivity in direct mycological exams and cultures. positive culture was considered a definitive diagnosis of fungal infection and confirmed 47.8% of diagnostic hypotheses.
Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis
Batista Júnior, Jo?o;Berzaghi, Rodrigo;Arnaud, Adelita Duanra Medeiros do Monte;Fontes, Cor Jesus Fernandes;Camargo, Zoilo Pires de;Hahn, Rosane Christine;
Memórias do Instituto Oswaldo Cruz , 2010, DOI: 10.1590/S0074-02762010000100009
Abstract: this study is the first report on genetic differences between isolates of paracoccidioides brasiliensis from a single patient. we describe a simultaneous infection with genetically distinct isolates of p. brasiliensis in a patient with chronic paracoccidioidomycosis. the clinical isolates were obtained from lesions in different anatomical sites and were characterised by random amplified polymorphic dna (rapd) analysis. the rapd technique can be helpful for distinguishing between clinical isolates. different random primers were used to characterise these clinical isolates. the rapd patterns allowed for differentiation between isolates and the construction of a phenetic tree, which showed more than 28% genetic variability in this fungal species, opening new possibilities for clinical studies of p. brasiliensis. based on these results and preliminary clinical findings, we suggest that different genotypes of p. brasiliensis might infect the same patient, inducing the active form of the disease.
Primeira descri??o da caracteriza??o fenotípica e susceptibilidade in vitro a drogas de leveduras do gênero Cryptococcus spp isoladas de pacientes HIV positivos e negativos, Estado de Mato Grosso
Favalessa, Olivia Cometti;Ribeiro, Luciano Correa;Tadano, Tomoko;Fontes, Cor Jesus Fernandes;Dias, Flávio Basili;Coelho, Bruno Pereira Albuquerque;Hahn, Rosane Christine;
Revista da Sociedade Brasileira de Medicina Tropical , 2009, DOI: 10.1590/S0037-86822009000600010
Abstract: thirty-seven isolates from 10 hiv-negative and 26 hiv-positive patients in mato grosso were evaluated. direct examination, culturing and chemotyping of species were performed. ketoconazole, itraconazole, voriconazole, fluconazole and amphotericin b were evaluated. thirty-seven yeasts of cryptococcus spp were identified, of which 26 were from hiv-positive patients (25 cryptococcus neoformans and one cryptococcus gattii) and 10 from hiv-negative patients (five cryptococcus neoformans and five cryptococcus gattii). the cryptococcus neoformans clinical isolates from hiv-positive patients showed resistance (8% and 8.7%) and dose-dependent susceptibility (20% and 17.4%) to fluconazole and itraconazole, respectively. among the cryptococcus neoformans isolates from hiv-negative patients, there was dose-dependent susceptibility (40%) to fluconazole. cryptococcus gattii isolates from hiv-negative patients were shown to be susceptible to all antifungal agents, except for one isolate of cryptococcus gattii that showed dose-dependent susceptibility to fluconazole (20%). the cryptococcus gattii isolate from an hiv-positive patient showed resistance to fluconazole (mic > 256 ?g/ml) and itraconazole (mic = 3 ?g/ml).
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
Gregory Gegembauer,Leticia Mendes Araujo,Edy Firmina Pereira,Anderson Messias Rodrigues,Anamaria Mello Miranda Paniago,Rosane Christine Hahn,Zoilo Pires de Camargo
PLOS Neglected Tropical Diseases , 2014, DOI: 10.1371/journal.pntd.0002986
Abstract: Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. In western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests.
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