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Search Results: 1 - 10 of 29397 matches for " Rong Han "
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The Evolution of Microtubule End-Binding Protein 1 (EB1) and Roles in Regulating Microtubule Behavior  [PDF]
Jiayu Liu, Rong Han
American Journal of Plant Sciences (AJPS) , 2015, DOI: 10.4236/ajps.2015.613212
Abstract: All organisms must transmit genetic information to offspring through cell division, and mitotic spindle participates in the process. Spindle dynamics through depolymerization or polymerization of microtubules generates the driving force required for chromosome movements in mitosis. To date, studies have shown that microtubule arrays control the directions of cell division and diverse microtubule-associated proteins regulate cell division. But a clear picture of how microtubules and microtubule-associated proteins modulate cell division remains unknown. Depletion of end-binding protein 1 by RNA-mediated inhibition shows that one of the microtubule-associated proteins, end-binding protein 1, plays a crucial role in mitotic spindle formation and promotes microtubule dynamics and is needed for the proper segregation of mitotic chromosomes during anaphase in Drosophila cells. Here, we review the properties of end-binding protein 1 and the roles of end-binding protein 1 in regulating microtubule behavior and in cell cycle.
Effects of Exogenous Nitric Oxide on Wheat Exposed to Enhanced Ultraviolet-B Radiation  [PDF]
Liyan Yang, Rong Han, Yi Sun
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.46159
Abstract:

We explored the use of exogenous nitric oxide (NO) on alleviating effects of UV-B light on winter wheat development. Triticum aestivum L. cv. Linyou 7287 seeds were irradiated with UV-B (10.08 kJ·m2·d–1) (enhanced UV-B) and watered with either water or 100 μmol·L–1 SNP solution. Plants were also watered with the SNP alone. The results showed that enhanced UV-B produced negative effects on seedling development. Leaf length decreased and seedling biomass dropped significantly compared with the control. Photochemical efficiency (Fv/Fm) dropped, and chlorophyll and carotenoid content as well as the ATPase activity declined. Content of UV-absorbing compounds and activity of the POD increased compared to the control. Application of the SNP, a NO donor partially protected wheat seedlings exposed to elevated UV-B radiation in that their leaf lengths and biomass accumulation were enhanced compared to the UV-B treatment alone. SNP also improved the contents of chlorophyll, carotenoid and UV-absorbing compounds in leaves. ATPase activity was enhanced but no influence on POD activity. Furthermore, the application of SNP alone showed a favorable effect on seedling growth compared with the control.

The Effects of He-Ne Laser and Enhanced Ultraviolet-B Radiation on Proliferating-Cell Nuclear Antigen in Wheat Seedlings  [PDF]
Feifeng Liu, Huize Chen, Rong Han
American Journal of Plant Sciences (AJPS) , 2015, DOI: 10.4236/ajps.2015.68124
Abstract: The level of ultraviolet-B (UV-B) radiation on the Earth’s surface has increased due to depletion of the ozone layer. Here, we explored the effects of continuous wave He-Ne laser irradiation (632 nm, 5 mW·mm-2, 2 min·d-1) on proliferating-cell nuclear antigen (PCNA) damage repair function of wheat seedlings exposed to enhanced UV-B radiation (10.08 kJ·m-2·d-1) at the early growth stages. Wheat seedlings were irradiated with enhanced UV-B, He-Ne laser treatment or a combination of the two. We explored the transcripts of PCNA in each treatment group using RT-PCR. In addition, total proteins were extracted from the 7-day-old wheat leaves, analyzed by SDS-PAGE and identified by western blot. The results showed that the transcription of PCNA was weakened following UV-B radiation compared to the control. However, when seedlings were subjected to elevated UV-B-damaging radiation followed by He-Ne laser irradiation, the expression of PCNA was signifi-cantly higher than UV-B radiation alone. These results suggest that He-Ne laser has an active role in repairing the UV-B damaging effects. In order to further investigate the function of PCNA, dynamic arrangements of PCNA in wheat root-tip cells were observed with confocal laser scanning microscopy (CLSM). The PCNA was marked fluorescent dimming and strength weakened in en-hanced UV-B radiation (UV-B) compared with the control group (CK) during processing. It shows that PCNA may be involved in the separation of chromosomes.
Different Doses of the Enhanced UV-B Radiation Effects on Wheat Somatic Cell Division  [PDF]
Feifeng Liu, Huize Chen, Rong Han
CellBio (CellBio) , 2015, DOI: 10.4236/cellbio.2015.42004
Abstract: Being sessile, plants are continuously exposed to DNA-damaging agents presenting in the environment such as ultraviolet (UV). Sunlight acts as an energy source for photosynthetic plants; hence, avoidance of UV radiations (namely, UV-A, 315 - 400 nm; UV-B, 280 - 315 nm; and UV-C, <280 nm) is unpreventable. DNA in particular strongly absorbs UV-B; therefore, it is the most important target for UV-B induced damages. This paper mainly used different doses of the enhanced UV-B radiation (B1 group: 4.05 kJ•m-2•d-1, B2 group: 10.08 kJ•m-2•d-1, B3 group: 7.05 kJ•m-2•d-1, B4 group: 23.02 kJ•m-2•d-1) treatment wheat, then, explored on the growth of wheat root and wheat root tip cell of chromosome aberration effect. In wheat, root-tip cells were observed with confocal laser scanning microscopy (CLSM), the results showed that low doses of B1 group (4.05 kJ•m-2•d-1) promoted the growth of wheat root and cell mitosis frequency. But high dose of B2 group (10.08 kJ•m-2•d-1), B3 group (17.05 kJ•m-2•d-1), B4 group (23.02 kJ•m-2•d-1) inhibited the growth of wheat root tip, and made crooked growth of wheat root, and inhibited the wheat root tip cell mitotic frequency and processed that induce root tip cells of wheat produce all kinds of aberration of chromosome in the interphase containing “multiple nucleoli nuclei”, “incomplete nuclei”, “long round nuclei”, “bean sprouts nucleus”. In mitosis M period contains “dissociative chromosome”, “chromosome bridge”, “adhesion chromosome”, “multi-bundle divide”, “nuclear anomalies”. After, high doses of enhanced UV-B radiation treatment, most of the cell cycle anomaly concentrated in mitosis interphase. In mitosis M period, with UV-B radiation dose enhanced chromosome aberration rate was on the rise and the aberration types also increasing.
Chelation Therapy for Mercury Poisoning
Rong Guan and Han Dai
Biochemistry Insights , 2012,
Abstract: Chelation therapy has been the major treatment for heavy metal poisoning. Various chelating agents have been developed and tested for treatment of heavy metal intoxications, including mercury poisoning. It has been clearly shown that chelating agents could rescue the toxicity caused by heavy metal intoxication, but the potential preventive role of chelating agents against heavy metal poisoning has not been explored much. Recent paper by Siddiqi and colleagues has suggested a protective role of chelating agents against mercury poisoning, which provides a promising research direction for broader application of chelation therapy in prevention and treatment of mercury poisoning.
Chelation Therapy for Mercury Poisoning
Rong Guan,Han Dai
Biochemistry Insights , 2009,
Abstract: Chelation therapy has been the major treatment for heavy metal poisoning. Various chelating agents have been developed and tested for treatment of heavy metal intoxications, including mercury poisoning. It has been clearly shown that chelating agents could rescue the toxicity caused by heavy metal intoxication, but the potential preventive role of chelating agents against heavy metal poisoning has not been explored much. Recent paper by Siddiqi and colleagues has suggested a protective role of chelating agents against mercury poisoning, which provides a promising research direction for broader application of chelation therapy in prevention and treatment of mercury poisoning.
The Biologic Characteristics and Treatment of Non-Small Cell Lung Cancer in Non Smokers
Rong QIAO,Baohui HAN
Chinese Journal of Lung Cancer , 2009,
Abstract:
The Changes of the first-line Chemotherapy on Advanced non-small Cell Lung Cancer
Rong QIAO,Baohui HAN
Chinese Journal of Lung Cancer , 2008,
Abstract:
Effects of Cu2+ on Wheat Seedlings Exposed to Enhanced Ultraviolet-B Radiation  [PDF]
Liyan Yang, Zhaoqing Wang, Yuqi Hou, Rong Han, Yi Sun
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.520322
Abstract:

To explore the wheat seedling development and physiological responses under copper contamination and enhanced ultraviolet-B (UV-B) irradiation, 10 mg·L-1 CuCl2 solution was irrigated to Triticum aestivum L. cv. Linyuan 2069 one day after germination with or without ultraviolet-B (10.08 kJ m-2·d-1) light exposure, respectively. The results showed that Cu2+ and UV-B caused various adverse effects on wheat seedling development. Cu2+ hindered root development by significantly reducing root number, while UV-B dwarfed seedling height and decreased the leaf length. Chlorophyll content and activity of ATPase in thylakoid membrane of wheat leaves dropped significantly under enhanced UV-B while the activity of ATPase in plasma membrane of seedling root was significantly decreased in Cu2+ group. Relative electric conductivity of leaves significantly increased in both Cu2+ and UV-B groups, so did the biomass. We also observed that combined Cu2+ and UV-B showed more adverse effects on wheat seedlings than either of them alone except for root growth.

The Distribution and Morphology Alterations of Microfilaments and Microtubules in Mesophyll Cells and Root-Tip Cells of Wheat Seedlings under Enhanced Ultraviolet-B Radiation  [PDF]
Limei Gao, Yongfeng Li, Aihua Guo, Jingru Zhai, Rong Han
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.522358
Abstract:
The distribution and morphology alterations of microfilaments and microtubules in the mesophyll cells and root-tip cells of wheat seedlings, which had been radiated by enhanced ultraviolet-B (10.08 KJ·m-2·d-1), were examined through the confocal laser scanning microscope (Model FV1000, Olympus, Japan). Microtubule was labeled with an indirect immunofluorescence staining method, and microfilament was labeled with fluorescein isothiocyanate-phalloidin (FITC-Ph) as probes. The results indicated that microtubules in mesophyll cells, compared with the controls, would be depolymerized significantly, and dispersed randomly showing some spots or short rods in the cytoplasm, under the enhanced UV-B radiation condition. The microtubule bundles tended to be diffused, and the fluorescence intensity of that significantly decreased. The distribution pattern of microfilaments, which usually arranged parallelly in control cells, was broken up by enhanced UV-B radiation. We further investigated the distribution and morphology of microtubules in root-tip cells during every stage of cell division, and found that these aberrant phenomena of microtubules were often associated with abnormal cell division. Our findings suggested that the distribution, morphology and structure of cytoskeleton in mesophyll cells and root-tip cells of wheat seedlings would be affected by enhanced UV-B radiation, which might be related to abnormal cell division caused by enhanced UV-B radiation as an extracellular signal.
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