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Search Results: 1 - 10 of 184475 matches for " Rodrigo Cardoso de;Buzalaf "
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Plasma as an indicator of bone fluoride levels in rats chronically exposed to fluoride
Carvalho, Juliane Guimar?es de;Oliveira, Rodrigo Cardoso de;Buzalaf, Marília Afonso Rabelo;
Journal of Applied Oral Science , 2006, DOI: 10.1590/S1678-77572006000400005
Abstract: objective: this study evaluated the use of plasma, bone surface (periosteal) and whole bone as biomarkers of chronic fluoride (f) exposure. methods: forty male wistar rats were assigned to 4 groups (n=10/gr) that differed according to the f concentration they received in the drinking water. groups 1, 2, 3 and 4 received water containing 0 (control), 5, 15, and 50 mg f/l, respectively. the rats were killed at 120 days of age. plasma and femur were collected and analyzed for fluoride with the ion specific electrode by the direct method or after hexamethyldisiloxane-facilitated diffusion. data were tested for statistically significant differences by anova and linear regression (p<0.05). results: mean (± se) plasma f concentrations ranged from 0.030 ± 0.002 to 0.187 ± 0.013 (mg/ml). the concentrations in surface and whole bone ranged from 610 ± 32 to 4,693 222; and 647 ± 22 to 3,439 ± 134 μg/g, respectively. the surface/whole f concentration ratios were 0.941, 1.414, 1.173 and 1.377, for groups 1, 2, 3 and 4 respectively. for plasma and whole bone, the difference among all groups was statistically significant, except for group 2 compared to group 1. for bone surface, all groups differed from each other except for group 2 compared to group 3. a significant positive correlation was found between bone surface and whole bone f (r2=0.94), as well as between plasma and bone surface (r2=0.71) and plasma and whole bone (r2=0.74). conclusions: data suggest that both bone surface and whole bone are suitable biomarkers of chronic f exposure in rats and plasma may be used as indicator of bone fluoride levels.
Recovery of silver residues from dental amalgam
Pereira, Heloísa Aparecida Barbosa da Silva;Iano, Flávia Godoy;Silva, Thelma Lopes da;Oliveira, Rodrigo Cardoso de;Menezes, Manoel Lima de;Buzalaf, Marília Afonso Rabelo;
Journal of Applied Oral Science , 2010, DOI: 10.1590/S1678-77572010000200005
Abstract: dental amalgam residues are probably the most important chemical residues generated from clinical dental practice because of the presence of heavy metals among its constituents, mainly mercury and silver. objective: the purpose of this study was to develop an alternative method for the recovery of silver residues from dental amalgam. material and methods: the residue generated after vacuum distillation of dental amalgam for the separation of mercury was initially diluted with 32.5% hno3, followed by precipitation with 20% nacl. sequentially, under constant heating and agitation with naoh and sucrose, the sample was reduced to metallic silver. however, the processing time was too long, which turned this procedure not viable. in another sequence of experiments, the dilution was accomplished with concentrated hno3 at 90oc, followed by precipitation with 20% nacl. after washing, the pellet was diluted with concentrated nh4oh, water and more nacl in order to facilitate the reaction with the reducer. results: ascorbic acid was efficiently used as reducer, allowing a fast reduction, thus making the procedure viable. conclusions: the proposed methodology is of easy application and does not require sophisticated equipment or expensive reagents.
Tissue response to a membrane of demineralized bovine cortical bone implanted in the subcutaneous tissue of rats
Oliveira, Rodrigo Cardoso de;Menezes, Renato;Cestari, Tania Mary;Taga, Eulázio Mikio;Taga, Rumio;Buzalaf, Marília Afonso Rabelo;Granjeiro, José Mauro;
Brazilian Dental Journal , 2004, DOI: 10.1590/S0103-64402004000100001
Abstract: the treatment of persistent bone defects has encouraged the search for proper techniques or bone substitutes. in dentistry, a common problem in the treatment of periodontal bone defects is the growth of tissues within the lesion, such as the junctional epithelium, which impair regeneration of these tissues. guided tissue regeneration (gtr), based on the separation of the tissues by means of membranes or barriers, was developed in an attempt to improve periodontal regeneration. the aim of this study was to histologically evaluate the tissue response to a membrane of demineralized bovine cortical bone implanted in the subcutaneous tissue of rats. the study periods were 1, 3, 7, 15, 30 and 60 days after implantation. analysis of the histological sections demonstrated a moderate to intense inflammatory response at 1 and 3 days, moderate at 7 and 15 days, and almost absent at 30 and 60 days. resorption of the membrane began 15 days after implantation, and at 60 days only remnants could be detected in some animals. we concluded that the demineralized bovine cortical bone membrane was well tolerated by the tissues and is completely resorbed after 30-60 days by mononuclear cells and multinucleated giant cells, which disappear upon completion of the process.
Alkaline phosphatase activity in plasma and liver of rats submitted to chronic exposure to fluoride
Fernandes, Mileni da Silva;Iano, Flávia Godoy;Rocia, Vivian;Yanai, Marcela Mitsuko;Leite, Aline de Lima;Furlani, Tatiana Almeida;Buzalaf, Marília Afonso Rabelo;Oliveira, Rodrigo Cardoso de;
Brazilian Archives of Biology and Technology , 2011, DOI: 10.1590/S1516-89132011000600014
Abstract: the aim of this study was to compare the effect of fluoride (f) on alkaline phosphatase activity in the liver and plasma of the rats. four groups of male wistar rats (n=6), which received drinking water containing 5, 15 or 50 ppm f or deionized water (control) throughout the experiment were included in the study. the animals were euthanized and had their tissues and blood plasma collected for the analysis of fluoride and alkaline phosphatase. there was an increase in f concentration in most tissues in the animals treated with higher f concentrations, except for the heart. the alkaline phosphatase assay showed an increase in the activity in the liver and blood plasma of the animals treated with fluoride concentrations of 15 and 50 ppm (p<0.05). this study suggested that f at a concentration of 50 ppm in drinking water promotes increased the activity of alkaline phosphatase in the liver and blood plasma.
Nail and bone surface as indicators of acute exposure to fluoride in rats
Buzalaf, Marília Afonso Rabelo;Linardi, Myrna Maria;Carvalho, Juliane Guimar?es de;Cardoso, Vanessa Eid da Silva;Cury, Jaime Aparecido;
Journal of Applied Oral Science , 2004, DOI: 10.1590/S1678-77572004000400006
Abstract: this study evaluated the use of nails and bone surfaces as indicators of acute exposure to fluoride in rats. six groups (n=10/grp), aged 70 days, received, by gastrogavage, single doses of sodium fluoride containing 10, 35, 45, 60, 75 and 90 mg fluoride/kg body weight. the control group received deionized water. two hours after fluoride administration, the rats were killed and their plasma, nails (halves near to the growth end) and femur were collected. nail and plasma fluoride concentrations were analyzed with the electrode following hmds-facilitated diffusion. femur surface fluoride was removed from a circular area (4.52 mm2) by immersion in 0.5m hcl for 15 seconds, buffered with tisab and analyzed with the electrode. data were analyzed by anova and tukey's test (p<0.05) and by linear regression (p<0.001). average fluoride concentrations ranged from 55.4 to 91.5 mg/g for nails; from 0.019 to 6.937 mg/ml for plasma and from 617 to 2,394 mg/g for femur surface. fluoride concentrations in nails from experimental groups were not different from control. regarding plasma fluoride, all experimental groups differed from control, except the group that received the lowest dose. in respect to femur surface fluoride, only the group that received the highest dose differed from control. a strong correlation was found between plasma fluoride and the dose administered (r=0.736) and a medium correlation was found between femur surface and the dose administered (r=0.510). data suggest that two hours after an acute fluoride administration, nails and femur surface are not good indicators of fluoride exposure.
First report of predation on floral visitors by crab spiders on Croton selowii Baill. (Euphorbiaceae)
Novo, Reinaldo Rodrigo;Souza, Jefferson Thiago;Castro, Cibele Cardoso de;
Acta Botanica Brasilica , 2010, DOI: 10.1590/S0102-33062010000200029
Abstract: in the literature it has been extensively mentioned that crab spiders (araneae: thomisidae) prey on floral visitors of several plant species. here we present observations of croton selowii baill. (euphorbiaceae), a monoecious species harboring individuals of crab spiders in an area of coastal vegetation of pernambuco state, brazil. the species is visited by several invertebrate orders, and some of them were preyed upon by the spiders, mainly diptera species. the spiders rubbed the forelimbs within the flowers, which may constitute a strategy to camouflage these structures. croton selowii seems to represent a suitable foraging site for the spiders, because it has a generalist pollination system (thus being visited by a wide range of invertebrate species) and blooms in a period of low flower resource availability in the area.
Avalia??o de uma política de estabiliza??o de renda para a agricultura familiar
Rodrigues, Rodrigo Vilela;Castro, Eduardo Rodrigues de;Teixeira, Erly Cardoso;
Revista de Economia e Sociologia Rural , 2007, DOI: 10.1590/S0103-20032007000100007
Abstract: for decades, the brazilian agriculture sector has suffered strong income transfers to many of the country's other economic sectors, which caused the impoverishment of many brazilian farmers. government sponsored rural credit assistance programs have somewhat redressed this inequity and ameliorated the farmer's plight. however, due to the structure of these credit assistance programs, the majority of their benefits have gone to the country's largest agricultural producers, thereby increasing income disparity found between family and commercial farms. new policies are needed that actually assist the majority of those that work in the brazilian agriculture sector: the family farmers. this work evaluates effects of implementation of an income stabilization policy (isp) intended to support family farmers, focusing on cassava, milk, and bean producers. the evaluation is based on the costs and benefits of isp, with reference to the theory of economic surplus. this article also considers the policy's negative effect on the surplus generated by commercial producers. study results indicate that implementation of the proposed isp would increase the prices received for cassava, milk and beans, improve the income of brazilian family farmers producing these products, and lead to consumer gains that more than offset the losses suffered by large, commercial agriculture producers.
Practice of teaching in (micro)history and cinematographic language: the short-length movie as ‘another’ historical narrative in and at the place
Humberto Perinelli Neto,Rodrigo Ribeiro Paziani,Rafael Cardoso de Mello
Educa??o : Teoria e Prática , 2012,
Abstract: This article has the objective to rethink concepts, contents and objectives of themes connected with the area of History through the articulation between the use of audiovisual languages – in case, the cinema – the role of the narrative and the construction of practices of teaching, all based on the studies of micro-history. We do not want to discuss only the educational and empirical potential of the technological resources inside the classrooms, but, specially, we want to understand the possible and real ways of transformation of the students, when they turn into subjects who creates new knowledges, by making short-length movies. This pedagogic tool and technological can help us think about the PCN's prerogatives, like, the increase in value of the place and of the daily life (of the pupils), the respect to the diversity and plurality, the role of the subjects in History. The proposal here presented is a result of a project carried out at the Funda o Educacional de Fernandópolis between 2009 and 2010, as works of conclusion of course developed by pupils of the degree course in History.
Morfogênese e acúmulo de forragem em pastagem de Cynodon dactylon cv. coastcross em diferentes esta??es de crescimento
Vilela, Duarte;Paiva, Paulo César de Aguiar;Lima, Josiane Aparecida de;Cardoso, Rodrigo Carvalho de;
Revista Brasileira de Zootecnia , 2005, DOI: 10.1590/S1516-35982005000600012
Abstract: the experiment was conducted to estimate morphogenic and structural characteristics and dry matter accumulation rate of leaf blades in a pasture of cynodon dactylon cv. coast-cross grass intermittently grazed by lactating cows. the evaluations were performed in spring (october-november), in summer (february-march) and in fall (may-june). the study was conducted observing a completely randomized design. with six replications. leaf length, appearance and senescence in marked tillers were registered over the rest period of the paddock. tillers in sampling areas of 0.09 m2 were counted for estimation of the tiller population density. the net dm accumulation rate of leaf blades was obtained by multiplying the dry matter accumulation rate by the number of tillers/area. leaf elongation rate was higher in the summer; while leaf appearance rate and the number of leaves/tiller were higher in spring. tiller population did not vary with season, averaging 2,770 tillers/m2. the senescence rate averaged 2.44 mm/day in the spring and no leaf senescence was observed over the summer and fall. dry matter accumulation rate of leaf blades was higher in the summer and showed a close relationship with leaf elongation rate and leaf size. leaf blade accumulation rate was overestimated when calculated from leaf elongation rate and tiller density data.
Síndrome de Parsonage-Turner: relato de caso em paciente HIV soropositivo
Oliveira, Saulo Gomes de;Pombo, Eduardo Hosken;Batista, Priscila Rossi de;Cardoso, Igor Machado;Rezende, Rodrigo;
Revista Brasileira de Ortopedia , 2010, DOI: 10.1590/S0102-36162010000400020
Abstract: the parsonage-turner syndrome is a rare disease that affects the muscles of the scapular girdle, leading to muscular atrophy and a large motor deficit. the etiology is unknown, but it is believed that infectious and autoimmune factors are involved. the diagnosis is made by exclusion, and the main differential diagnoses are cervical disc hernias, rotator cuff injuries and rheumatic diseases. during diagnostic research, we conducted laboratory tests, radiographs and mri of the shoulder and cervical spine, with particular reference to electroneuromyography to help generate a definitive diagnosis. this case report is presented because it shows a disease that is rarely associated with hiv seropositivity and the importance of early diagnosis for better treatment of these patients.
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