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Search Results: 1 - 10 of 9423 matches for " Robson Maia;Nascimento "
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Lingui?as frescais elaboradas com carne de avestruz: características físico-químicas
Nascimento, Rafael Soares;Fonseca, Ana Beatriz Monteiro;Franco, Robson Maia;Miranda, Zander Barreto;
Ciência Rural , 2012, DOI: 10.1590/S0103-84782012000100030
Abstract: ostrich's meat has low contents of colestherol and intramuscular fat and high percentages of poliunsaturated fat acid -3 so it's known as a healthy red meat with consumer great acceptability. considering that ostrich trim (meat resulting from boning) is considered a less noble part, three formulations of ostrich (trim) brazilian sausage were produced. physicochemical analyses and color evaluation (l*, a*, b*) were made to determine formulations' physicochemical characteristics and colorimetric profile. the physicochemical results of all formulations were in accord with the brazilian legislation pattern's (brasil, 2000) and no differences (p>0,05) between formulations have been found except in the ash content, higher in mixed sausages. differences (p>0,05) have been found also between the ph of the treatments, which increased with the heightening of the percentage of ostrich meat in formulation. the sausages formulated with 100% ostrich meat were darker and redder in appearance than the mixed ones. the mixed of ostrich, pork and chicken brazilian sausage were the lightest. the brazilian sausages prepared with ostrich meat, pure or mixed, are a viable option for production and sale, and can be placed in the market and compete with the traditional sausages with the prospect of being successful.
Perfil de sensibilidade antimicrobiana e detec??o do gene ISS pela rea??o em cadeia da polimerase na tipifica??o de Escherichia coli patogênica em codornas de corte sob inspe??o sanitária
Abreu, Dayse Lima da Costa;Franco, Robson Maia;Nascimento, Elmiro Rosendo do;Pereira, Virginia Léo de Almeida;Alves, Fernanda Martinez Xavier;Almeida, Juliana Ferreira de;
Pesquisa Veterinária Brasileira , 2010, DOI: 10.1590/S0100-736X2010000500006
Abstract: the pathogenicity of escherichia coli strains is partially related to the expression of virulence factors genes, present in genetic elements called plasmids. apec strains responsible for diseases in birds may present the iss gene which increases the resistance of e. coli strains to the lityc effect of the host's serum, besides resistance to several antimicrobials. this study was conduced in order to detect e. coli in tracheae of meat-type quails and to evaluate, by the presence of the iss gene and the profile of antimicrobial susceptibility, the pathogenic potential of the isolated samples for birds and humans. one hundred and eighty tracheae of quails were collected for detection of e. coli, antimicrobial sensitivity tests, and for polymerase chain reaction (pcr), for detection of iss gene. from the examined quails, 8.9 % (16/180) were positive for e. coli, from which 20 strains of this bacterium were obtained. most of them were resistant to tetracycline (16/20), followed by ceftadizime (13/20) and nalidixic-acid (12/20) and only one isolate was resistant to amoxicillin. the detection of iss gene occurred in 55% (11/20) of the isolates, indicating that these strains had the potential to be pathogenic not only for quails, but also for other kinds of birds, other animals and even human beings that would be in contact with these e. coli isolates.
Laminas de irriga??o e genótipos na produca??o e qualidade de frutos de bananeira
Braga Filho, Juracy Rocha;Nascimento, Jorge Luiz do;Naves, Ronaldo Veloso;Torres, Maria Célia Lopes;Geraldine, Robson Maia;Souza, Eli Regina Barboza de;Barroso, Flávio Vieira;
Pesquisa Agropecuária Tropical , 2011, DOI: 10.5216/pat.v41i2.9041
Abstract: brazil is the fourth biggest banana producer. however, its social function is more important than the economic one. in this study, the productive features of banana tree genotypes, under different irrigation levels, and the sensorial characteristic for taste of ripe fruits were evaluated. the experiment was carried out from january 2004 to july 2005, with the following genotypes: false fhia 18, grand naine, prata, and thap maeo. after resting for two days in a cold chamber, the fruits were evaluated in the third, fourth and fifth days, for determining the preferred sample. the four genotypes presented significant results to irrigation. the water levels effect was verified in the weight of the bunch, rachis, and hands, and in the total number of fruits, bunch length and number of hands. the weight of the bunch and hands, and the total number of fruits, in the false fhia 18, grand naine, and thap maeo genotypes, presented similar responses, except for the weight of bunch and hands, in thap maeo. the effect of the water levels among the genotypes occurred on most variables, except for the weight and the number of fruits of the second hand and diameter of the central fruit. false fhia 18 presented more variables influenced by the irrigation levels and, for prata, the only influence occurred in the number of hands. the irrigation positively influenced the productive characteristics of the false fhia 18, grand naine, and thap maeo banana trees, which showed to be promising for the brazilian savannah region. consumers prefer the prata and grand naine genotypes.
Identification of mechanisms involved in the relaxation of rabbit cavernous smooth muscle by a new nitric oxide donor ruthenium compound
Cerqueira, Jo?o Batista Gadelha de;Gonzaga-Silva, Lúcio Flávio;Silva, Francisco Ordelei Nascimento da;Cerqueira, Jo?o Victor Medeiros de;Oliveira, Ricardo Reges Maia;Moraes, Maria Elisabete Amaral de;Nascimento, Nilberto Robson Falc?o do;
International braz j urol , 2012, DOI: 10.1590/S1677-55382012000500015
Abstract: purpose: the aim of this study was to evaluate the relaxation in vitro of cavernous smooth muscle induced by a new no donor of the complex nitrosil-ruthenium, named trans-[ru(nh3)4(caffeine)(no)]c13 (rut-caf) and sodium nitroprusside (snp). materials and methods: the tissues, immersed in isolated bath systems, were pre-contracted with phenilephrine (pe) (1 μm) and then concentration-response curves (10-12 - 10-4 m) were obtained. to clarify the mechanism of action involved, it was added to the baths odq (10 μm, 30 μm), oxyhemoglobin (10 μm), l-cysteine (100 μm), hydroxicobalamine (100 μm), glibenclamide, iberotoxin and apamine. tissue samples were frozen in liquid nitrogen to measure the amount of cgmp and camp produced. results: the substances provoked significant relaxation of the cavernous smooth muscle. both rut-caf and snp determined dose-dependent relaxation with similar potency (pec50) and maximum effect (emax). the substances showed activity through activation of the soluble guanylyl cyclase (sgc), because the relaxations were inhibited by odq. oxyhemoglobin significantly diminished the relaxation effect of the substances. l-cysteine failed to modify the relaxations caused by the agents. hydroxicobalamine significantly diminished the relaxation effect of rut-caf. glibenclamide significantly increased the efficacy of rut-caf (pec50 4.09 x 7.09). there were no alterations of potency or maximum effect of the substances with the addition of the other ion channel blockers. rut-caf induced production of significant amounts of cgmp and camp during the relaxation process. conclusions: in conclusion, rut-caf causes relaxation of smooth muscle of corpus cavernosum by means of activation of sgc with intracellular production of cgmp and camp; and also by release of no in the intracellular environment. rut-caf releases the no free radical and it does not act directly on the potassium ion channels.
Percal?os na história da ciência: B. F. Skinner e a aceita??o inicial da Análise Experimental do comportamento entre as décadas de 1930 e 1940
Cruz, Robson Nascimento da;
Psicologia: Teoria e Pesquisa , 2011, DOI: 10.1590/S0102-37722011000400020
Abstract: the initial draft of the concept of operant conditioning and of the single-subject experimental design defines the bases of skinner's explanatory system. however, these formulations were not immediately welcomed. to enable a closer look on the history of formation of behavior analysis, three historical facts were highlighted: (a) the difficulties faced by skinner at the end of his post-graduate studies; (b) the reaction to his first book, the behavior of organisms, and (c) the dispute with other explanatory behaviorial models. the conclusion of the present study is that the history of formation of behavior analysis is constituted by determinants of motivational, institutional, emotional and economic nature and by difficulties of going against the dominant methodological trends of north-american experimental psychology.
Possíveis rela??es entre o contexto histórico e a recep??o do behaviorismo radical
Cruz, Robson Nascimento da;
Psicologia: Ciência e Profiss?o , 2010, DOI: 10.1590/S1414-98932010000300004
Abstract: this article discusses the reception of radical behaviorism, especially in the 70’s, and tries to link the controversy around some of skinner’s concepts disclosed in his book beyond freedom and dignity. the debate has been guided by three sets of issues: a) the incompatibility between the historical moment and the proposal of radical behaviorist notions of freedom and dignity that explain ways to control the behavior which are not traditionally assumed; b) the problems in identifying the radical behaviorism as compatible with the assumptions of liberalism; c) the language used by skinner as a source of distortions. finally, we discuss the limitations and problems involved in the attempts to clarify the possible misunderstandings about radical behaviorism. at the same time, it is shown that the reception of radical behaviorism is pervaded by issues beyond those related to the internal validity of the skinnerian explanatory system, and disagreement about this approach can not always be explained as ignorance and misconceptions about it.
CONTAMINA O POR AFLATOXINAS EM HíBRIDOS DE MILHO CULTIVADOS EM TRêS REGI ES DO ESTADO DE GOIáS
Cristiane Regina Ramos,Edward Madureira Brasil,Robson Maia Geraldine
Pesquisa Agropecuária Tropical , 2008,
Abstract: A ocorrência natural de aflatoxinas G1(AFG1), G2(AFG2), B1 (AFB1) e B2 (AFB2) em gr os de milho foi avaliadaem três locais do Estado de Goiás (Jataí, Montividiu e Goiania),a partir de amostras de doze híbridos (DAS766, DAS657, 30K75,30F44, 30P70, 3F33, AG7000, DKB350, AG1051, Strike, Speede Fort). A análise de aflatoxinas foi realizada por cromatografialíquida de alta eficiência (CLAE), correlacionando-se os resultadoscom dados de temperatura e chuvas nas regi es, e com a presen ade fungos e gr os ardidos nas amostras. Em Goiania, verificou-sea menor ocorrência de gr os ardidos, havendo diferen asignificativa entre os híbridos. A análise patológica dos gr os foirealizada pelo teste "Blotter". Detectou-se a presen a deAspergillus spp. em todas as amostras provenientes de Jataí, em41,7% das amostras de Goiania e em apenas uma amostra deMontividiu. O local onde houve maior contamina o poraflatoxinas foi Jataí, seguido de Goiania e Montividiu,respectivamente. Em Jataí, esse resultado foi correlacionado coma maior quantidade de chuvas durante a colheita. Os níveis decontamina o variaram entre "n o detectada" (nd) e 277,8 μg.kg-1, para AFB1; de 0,7 μg.kg-1 a 14 μg.kg-1, para AFB2; e de nda 34,1 μg .kg-1, para AFG2. N o foi detectada a aflatoxina G1 emquaisquer das amostras analisadas.
AVALIA O DE MéTODOS DE EXTRA O, LIMPEZA E PURIFICA O DE AFLATOXINAS PARA ANáLISE EM CROMATOGRAFIA LíQUIDA DE ALTA EFICIêNCIA EVALUATION OF EXTRACTION, CLEANING AND PURIFICATION METHODS OF AFLATOXINS FOR HPLC ANALYSIS
Cristiane Regina Ramos,Edward Madureira Brasil,Robson Maia Geraldine
Pesquisa Agropecuária Tropical , 2008, DOI: 10.5216/pat.v38i2.4158
Abstract: Foi realizada avalia o comparativa de dois métodos de extra o, limpeza e purifica o de aflatoxinas, para análise em cromatógrafo líquido de alta eficiência. Um dos métodos é físicoquímico e baseia-se em rea es químicas para todas as etapas. O outro utiliza coluna de imunoafinidade. Os métodos foram avaliados quanto à porcentagem de recupera o, custo do processo, tempo gasto para análise e número de amostras analisadas por dia. A recupera o obtida pelo método físicoquímico foi mais baixa do que a do método de imunoafinidade, para as aflatoxinas B1, B2 e G1, porém mais alta para G2. Ambos os métodos apresentaram valores de recupera o dentro da faixa estabelecida pela Comunidade Européia, que vai de 70% a 120%. A análise comparativa de custos revelou que estes foram menores no método físico-químico. Porém, suas análises s o demoradas, por envolverem várias etapas, e exigem maior cautela por parte do laboratorista. Apesar do custo mais elevado, aproximadamente seis vezes superior ao do método físicoquímico, o método de imunoafinidade apresenta maior rapidez e facilidade de execu o. PALAVRAS-CHAVE: Recupera o; Aflatoxinas; métodos de extra o; imunoafinidade; CLAE. A comparative evaluation process was carried out between two methods of aflatoxins extraction, cleaning, and purification, for High Performance Liquid Chromatography (HPLC) analysis. The first one is the physical-chemical method, based on chemical reactions for all the stages, and the second makes use of a immunoaffinity column (IAC). The methods were evaluated as related to recovery percentage, cost, time spent for analysis, and number of samples analyzed per day. The recovery percentage obtained through the physical-chemical method was lower than the IAC method to B1, B2 and G1 aflatoxins, and higher to G2 aflatoxin. Both techniques presented recovery percentages in accordance with established values by the European Community (70% to 120%). Concerning costs, the physical-chemical method showed to be the least expensive, but its analysis process takes more time, because it involves more steps and demands more caution from laboratory technicians. Despite its higher cost, about six times higher than the physicalchemical method, the IAC method showed to be faster and easier to perform. KEY-WORDS: Recovery; aflatoxins; extraction methods; immunoaffinity; HPLC.
AFLATOXINS CONTAMINATION OF CORN HYBRIDS IN THREE REGIONS OF THE GOIáS STATE, BRAZIL CONTAMINA O POR AFLATOXINAS EM HíBRIDOS DE MILHO CULTIVADOS EM TRêS REGI ES DO ESTADO DE GOIáS
Cristiane Regina Ramos,Edward Madureira Brasil,Robson Maia Geraldine
Pesquisa Agropecuária Tropical , 2008, DOI: 10.5216/pat.v38i2.4157
Abstract: The natural occurrence of aflatoxins B1 (AFB1), B2 (AFB2), G1(AFG1), and G2 (AFG2) in corn grains was evaluated in three locations of Goiás State (Jataí, Montividiu and Goiania), from twelve grain samples of hybrids (DAS766, DAS657, 30K75, 30F44, 30P70, 30F33, AG7000, DKB350, AG1051, Strike, Speed and Fort). The aflatoxins were analyzed by high performance liquid chromatography (HPLC). The results were related to the temperature and rainfall data from each region, and to the presence of fungi and rotten ears in the samples. The lowest percentage of rotten ears occurred in Goiania, with a significant difference among the hybrids. The pathological analysis of the seeds was determined by the "Blotter" test. The Aspergillus spp. was present in all the samples from Jataí, in 41.7% of the samples from Goiania, and a single sample from Montividiu. The aflatoxin contamination was higher in the samples from Jataí, followed by Goiania and Montividiu, respectively. In Jataí, that result was correlated with higher rainfall levels during harvest. The levels of AFB1 ranged between "not detected" (nd) and 277.8 μg.kg-1; 0.7 μg.kg-1, and 14 μg.kg-1, for AFB2; and between nd and 34.1 μg.kg-1, for AFG2. The AFG1 was not detected in any of the samples. KEY-WORDS: Rotten ear; Aspergillus spp.; aflatoxin; maize; HPLC. A ocorrência natural de aflatoxinas G1(AFG1), G2 (AFG2), B1 (AFB1) e B2 (AFB2) em gr os de milho foi avaliada em três locais do Estado de Goiás (Jataí, Montividiu e Goiania), a partir de amostras de doze híbridos (DAS766, DAS657, 30K75, 30F44, 30P70, 3F33, AG7000, DKB350, AG1051, Strike, Speed e Fort). A análise de aflatoxinas foi realizada por cromatografia líquida de alta eficiência (CLAE), correlacionando-se os resultados com dados de temperatura e chuvas nas regi es, e com a presen a de fungos e gr os ardidos nas amostras. Em Goiania, verificou-se a menor ocorrência de gr os ardidos, havendo diferen a significativa entre os híbridos. A análise patológica dos gr os foi realizada pelo teste "Blotter". Detectou-se a presen a de Aspergillus spp. em todas as amostras provenientes de Jataí, em 41,7% das amostras de Goiania e em apenas uma amostra de Montividiu. O local onde houve maior contamina o por aflatoxinas foi Jataí, seguido de Goiania e Montividiu, respectivamente. Em Jataí, esse resultado foi correlacionado com a maior quantidade de chuvas durante a colheita. Os níveis de contamina o variaram entre "n o detectada" (nd) e 277,8 μg.kg-1, para AFB1; de 0,7 μg.kg-1 a 14 μg.kg-1, para AFB2; e de nd a 34,1 μg .kg-1, para AFG2. N o foi detectada a aflatoxina
Modelo de vegeta??o potencial para a Regi?o Nordeste decorrente da precipita??o pluvial
Nascimento, Robson de Sousa;Brito, José Ivaldo Barbosa de;
Bragantia , 2007, DOI: 10.1590/S0006-87052007000300019
Abstract: the present work applies the model of rey to calculate the potential vegetation of the northeast of brazil and, thus, to verify if it properly identifies the biomes of that region. the method used to calculate the rate of evapotranspiration (et) is based on the equation of penman-monteith. the model also includes fixed parameters and the variables vegetation height and leaf area index (lai). in general, the model provided a good representation of the biomes of the northeast of brazil, mainly the caatinga, the cerrado, and the atlantic forest.
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