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Search Results: 1 - 10 of 185803 matches for " Renato de Oliveira;Buso "
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Shoot tip culture and thermotherapy for recovering virus-free plants of garlic
Torres, Antonio Carlos;Fajardo, Thor Vinícius;Dusi, André Nepomuceno;Resende, Renato de Oliveira;Buso, José Amauri;
Horticultura Brasileira , 2000, DOI: 10.1590/S0102-05362000000300010
Abstract: garlic shoot tip culture associated with dry heat thermotherapy (cloves exposed to 37°c for 35 days) were essential for recovering virus free plants of the cv amarante. in this condition 70% of the explants developed in vitro and produced plants. a total of 77% of those plants was virus free when indexed by isem, which resulted in a final index of 54% of virus free plants from treated cloves. the percentage of regeneration decreased to 20% as the temperature increased up to 40°c. however 90% of those plants were virus free, leading to a final index of 18% virus free plants out of treated cloves.
Detection of three Allexivirus species infecting garlic in Brazil
Melo Filho, Péricles de Albuquerque;Nagata, Tatsuya;Dusi, André Nepomuceno;Buso, José Amauri;Torres, Antonio Carlos;Eiras, Marcelo;Resende, Renato de Oliveira;
Pesquisa Agropecuária Brasileira , 2004, DOI: 10.1590/S0100-204X2004000800002
Abstract: garlic viruses often occur in mixed infections under field conditions. in this study, garlic samples collected in three geographical areas of brazil were tested by dot-elisa for the detection of allexiviruses using monoclonal specific antibodies to detect garlic virus a (garv-a), garlic virus b (garv-b), garlic virus c (garv-c) and a polyclonal antiserum able to detect the three virus species mentioned plus garlic virus d (garv-d). the detected viruses were biologically isolated by successive passages through chenopodium quinoa. reverse transcriptase polimerase chain reaction (rt-pcr) was performed using primers designed from specific regions of the coat protein genes of japanese allexiviruses available in the genetic bank of national center of biotechnology information (ncbi). by these procedures, individual garlic virus genomes were isolated and sequenced. the nucleotide and amino acid sequence analysis and the one with serological data revealed the presence of three distinct allexiviruses garv-c, garv-d and a recently described allexivirus, named garlic mite-borne filamentous virus (garmbfv), in brazil.
PREVALENCE OF HEPATITIS B AND HEPATITIS C MARKERS IN ALCOHOLICS WITH AND WITHOUT CLINICALLY EVIDENT HEPATIC CIRRHOSIS
OLIVEIRA Luiz Carlos Marques de,BUSO Abadia Gilda,OLIVEIRA Alessandra Terezinha Rodrigues de,ARANTES Cidamaiá Aparecida
Revista do Instituto de Medicina Tropical de S?o Paulo , 1999,
Abstract: We assessed the frequency of serological markers of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections in 365 alcoholics by determining, by ELISA, the presence of HBsAg, anti-HBc, anti-HBs and anti-HCV. Fifty patients were cirrhotics and 315 had no evidence of hepatic cirrhosis; of the latter HBsAg was assessed in all, anti-HBc and anti-HBs in 130, and anti-HCV in 210. Among the alcoholics the frequencies of HBsAg (1.9%), anti-HBc (28.3%) and anti-HCV (3.8%) were higher (p<0.001) than among the controls (N=17,059), 0.4%, 4.0% and 0.4% respectively. The frequency of positive HBsAg was higher (p<0.001) in the cirrhotic patients (8.0%) than in alcoholics without cirrhosis (0.95%) and in controls (0.4%), and similar between the latter; of anti-HBc in alcoholics without cirrhosis (28.5%) was similar in cirrhotics patients (28.0%) and higher (p<0.001) than in the controls (4.0%); of anti-HBs in alcoholics without cirrhosis (20.8%) was similar to that of the cirrhotic patients (10.0%), and the anti-HCV was similar between alcoholics with (6.0%) and without cirrhosis (3.3%) and higher (p<0.001) than in controls (0.4%). We concluded that: a) alcoholics with or without cirrhosis have similar frequencies of infection with HBV and HCV between them, and higher than in nonalcoholics; b) alcoholics without cirrhosis had a frequency of HBV active infection (HBsAg+) which was similar to the controls, whereas among those who progressed to cirrhosis this frequency was significantly higher, what suggests that HBV may be implicated in the pathogenesis of cirrhosis in a few alcoholic individuals.
ética na escola: (re)acendendo uma polêmica
Oliveira, Renato José de;
Educa??o & Sociedade , 2001, DOI: 10.1590/S0101-73302001000300012
Abstract: considering that ethics at school and the education of students' character have been discussed all over the world, this paper focuses its subject based on three axes. the first one presents some philosophical views on morality and ethics in order to investigate whether or not theoretical distinctions have social impacts. the second axis is related to the foundations of morality and ethics as practical disciplines. the main question is: should general principles legitimate moral/ethical judgments or vice versa? finally the role assumed by school in the ethical/moral education of the citizen is explored. both educational conceptions as those owing to comenius and dewey and the homogenizing pedagogical practices are discussed, as well as the ethical proposals included in the parametros curriculares nacionais (national curricular guidelines)
Contribui??es da racionalidade argumentativa para a abordagem da ética na escola
Oliveira, Renato José de;
Educa??o e Pesquisa , 2012, DOI: 10.1590/S1517-97022011005000005
Abstract: this article discusses the work on ethics in school (primary and secondary education) with basis on a critique of the model of demonstrative rationality, prevalent since the advent of modernity. alternatively, it presents another model, the argumentative rationality one, which is discussed with basis on the approaches taken by cha?m perelman, lucie olbrechts-tyteca and michel meyer. the first two authors have formulated a theory of argumentation whose goal is to support the typical reasoning of human and social sciences, knowledge areas in which typical procedures of demonstrative rationality, such as deduction and calculation do not apply. meyer stresses the problematizing nature of argumentative rationality, placing the rhetoric as a means of negotiating the distance between individuals in regard to certain issues. this theoretical framework is complemented by the philosophy of pluralism appointed by perelman as a counterpoint to the worldviews grounded on unitary conceptions of truth, called monisms. in this theoretical framework, i discuss the universalistic ethical concept of kant and the relativism proposed by the ethics of aesthetics defended by maffesoli. in a second topic, ethics in school education is problematized in view of the question: how can one shape the character of the student without falling into some sort of indoctrination? to the extent that ethics and morality relate to this question, i discuss how the two terms have been understood and i propose to regard them as interchangeable instances. the conclusions i present based on these discussions aim not to solve the problems inherent in the habits and attitudes of students, but to contribute to teachers' reflection about their teaching practices.
ética na escola: (re)acendendo uma polêmica
Oliveira Renato José de
Educa??o & Sociedade , 2001,
Abstract: Considerando que a ética na escola e a forma o do caráter do aluno têm sido discutidas em todo mundo, este artigo trata o tema com base em três eixos. O primeiro apresenta algumas vis es filosóficas de ética e de moral, buscando investigar se as distin es teóricas têm ou n o significado social. O segundo eixo se relaciona aos fundamentos da ética e da moral como disciplinas práticas. A principal quest o colocada é: os princípios gerais devem legitimar os juízos de valor éticos/morais ou vice-versa? Finalmente, é abordado o papel cumprido pela escola na forma o ética/moral do cidad o. Certas concep es educacionais como a de Comênio, a de Dewey e também as práticas pedagógicas homogeneizadoras, assim como as propostas relativas à ética presentes nos Parametros Curriculares Nacionais, s o discutidas.
O Ensino de Ciências e a ética na Escola: Interfaces Possíveis
Renato José de Oliveira
Química Nova na Escola , 2010,
Abstract: Este artigo discute algumas quest es relativas ao ensino de química, física e biologia no nível médio, argumentandoque para compreender o baixo interesse dos alunos por essas disciplinas n o basta simplesmenteculpá-los, criticar a escola ou reclamar da incompetência dos professores. é necessário analisar, no contextoda cultura científica ocidental, os processos por meio dos quais antigas certezas se desfazem e novas afloram,refletindo-se ou n o no trabalho pedagógico escolar. Este, por sua vez, n o se dissocia da forma o do caráterdo aluno, de sorte que a discuss o acerca do bem, dos princípios morais e do agir ético se faz presente na escola, envolvendo gestores, docentes, orientadores educacionais, funcionários e estudantes. Assim sendo, entre os conhecimentos veiculados pela química, pela física e pela biologia e as quest es éticas da atualidade, é possível e desejável estabelecer interfaces que venham a enriquecer as práticas pedagógicas, trazendo ganhos para todos os envolvidos.
Deterritorialization and localization of universities. In search of a conceptual framework for planning higher education, taking into account regional development
Renato de Oliveira
Revista de Universidad y Sociedad del Conocimiento , 2007,
Abstract: Economical globalisation has changed the relationship between universities and economic development strategies. The various forms of direct cooperation between universities and organisations from the productive sector alter the internal profile of universities, forcing them to provide solutions that must be increasingly suited to the existing social demands.This situation opens new perspectives so that universities commit to the designing of regional development strategies. However, the many experiences underway reveal the need to overcome major obstacles so that this new role may be performed, thereby avoiding a situation in which these institutions succumb to the authoritarian tendencies represented by the financing of the economy on a global scale.
Peptic disease and Helicobacter pylori are highly prevalent in patients with the indeterminate form of chagas? disease: report of 21 cases
OLIVEIRA Luiz Carlos Marques de,BUSO Abadia Gilda,SIQUEIRA FILHO Luiz,MORAES Flávia
Revista do Instituto de Medicina Tropical de S?o Paulo , 1997,
Abstract: Given that chagasic patients in the indeterminate form of this disease, can have abnormal motility of the digestive tract and immunologic abnormalities, we decided to assess the frequency of peptic disease and Helicobacter pylori (Hp) infection in these individuals. Twenty-one individuals, 13 males and 8 females, mean age 37.6 ± 11.1 years, were examined. Biopsies of the duodenum, antrum, lesser and greater gastric curvature and esophagus were performed. The endoscopic findings were of chronic gastritis in 20 (95.2%) patients, duodenal ulcer in 3 (14.3%), gastric and duodenal ulcer in 3 (14.3%), gastric ulcer alone in 1 (4.8%), esophagitis in 5 (23.8%), and duodenitis in 5 (23.8%). The diagnosis of infection by the Hp was done by the urease test and histologic examination. Hp infection was found in 20 (95.2%) individuals: in 20 out of them in the antrum, in 17 in the lesser curvature, and in 17 in the greater curvature. Hp was not found in the esophagus and duodenum. The only individual with no evidence of infection by Hp was also the only one with normal endoscopic and histologic examinations. The histologic examinations confirmed the diagnoses of gastric ulcer as peptic, chronic gastritis in 20 patients, duodenitis in 14, and esophagitis in 9. In this series the patients had a high frequency of peptic disease, which was closely associated with Hp infection
Sealing ability of castor oil polymer as a root-end filling material
Martins, Giovana Ribeiro de;Carvalho, Claudio Antonio Talge;Valera, Marcia Carneiro;Oliveira, Luciane Dias de;Buso, Leonardo;Carvalho, Alessandra Sverberi;
Journal of Applied Oral Science , 2009, DOI: 10.1590/S1678-77572009000300017
Abstract: objective: the purpose of this study was to evaluate the sealing ability of castor oil polymer (cop), mineral trioxide aggregate (mta) and glass ionomer cement (gic) as root-end filling materials. forty-five single-rooted human teeth were cleaned and prepared using a step-back technique. the apical third of each root was resected perpendicularly to the long axis direction. all teeth were obturated with gutta-percha and an endodontic sealer. after, a root-end cavity with 1.25-mm depth was prepared using a diamond bur. the specimens were randomly divided into three experimental groups (n=15), according to the root-end filling material used: g1) cop; g2) mta; g3) gic. the external surfaces of the specimens were covered with epoxy adhesive, except the root-end filling. the teeth were immersed in rhodamine b dye for 24 hours. then, the roots were sectioned longitudinally and the linear dye penetration at the dentin/material interface was determined using a stereomicroscope. anova and tukey's tests were used to compare the three groups. the g1 group (cop) presented smaller dye penetration, statistically different than the g2 (mta) and g3 (gic) groups (p<0.05). no statistically significant difference in microleakage was observed between g2 and g3 groups (p>0.05). the results of this study indicate that the cop presented efficient sealing ability when used as a root-end filling material showing results significantly better than mta and gic.
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