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Search Results: 1 - 10 of 11246 matches for " Renato Vasconcelos;Maia "
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Plant regulators for rooting of hardwood cuttings of blackberry plants cv. Xavante/ Reguladores vegetais no enraizamento de estacas lenhosas da amoreira-preta cv. Xavante
Aline José Maia,Renato Vasconcelos Botelho
Semina : Ciências Agrárias , 2008,
Abstract: Aiming to evaluate the use of plant regulators for rooting of hardwood cuttings of blackberry cv. Xavante, two experiments were carried out in a greenhouse. In the first one the concentrations of indolil butyric acid varied in 0, 1000, 1500, 2000, 2500 and 3000 mg L-1. And in the second one, the plant regulator that was used was paclobutrazol at the concentrations of 0, 200, 400, 600, 800 and 1000 mg L-1. For each experiment the base of the cuttings were dipped in the solutions for ten seconds. After that, the cuttings were planted in rigid plastic vases with sand and kept under intermittent misty system. The following variables were evaluated, 71 days after planting: percentage of rooted cuttings, percentage of callus presence, average length of roots, number of roots, fresh weight of roots, percentage of sprouting. The treatment of hardwood cuttings with 2000 mg L-1 IBA had the greatest percentage of rooting (60%). Nevertheless, the treatment with 1000 mg L-1 IBA attained already high rooting percentage (56%), with 96% of sprouted cuttings and 17.6 roots per cutting. Cuttings treated with PBZ presented greater callus presence but they did not root, thus it is not possible to recommend this product for rooting of blackberry plants cv. Xavante among the tested concentrations. Com o objetivo de avaliar o emprego de reguladores vegetais no enraizamento de estacas lenhosas da amora-preta cv. Xavante dois experimentos foram conduzidos em casa-de-vegeta o. No primeiro, empregou-se o ácido indol butírico (AIB) nas concentra es de 0, 1000, 1500, 2000, 2500, 3000 mg L-1. No segundo, o regulador utilizado foi o paclobutrazol (PBZ) nas concentra es de 0, 200, 400, 600, 800, 1000 mg L-1. Em ambos os experimentos as estacas ficaram imersas por 10 segundos nas respectivas solu es. Posteriormente foram plantados em vasos de plástico rígido contendo areia, sendo mantidas em casade- vegeta o sob nebuliza o intermitente. Após 71 dias do plantio, foram avaliadas as seguintes variáveis: porcentagem de estacas enraizadas, comprimento médio das raízes, número de raízes, massa fresca de raízes, porcentagem de estacas com brota es e número de folhas. O tratamento de estacas lenhosas a 2000 mg L-1 AIB obteve a maior porcentagem de enraizamento (60%). No entanto, o tratamento a 1000 mg L-1 AIB já atingiu enraizamento elevado (56%), com 96% de estacas brotadas e 17,6 raízes por estaca. As Estacas tratadas com PBZ apresentaram maior presen a de calo e n o enraizaram n o sendo possível recomendar este produto para o enraizamento da amoreira-preta cv. Xavante nas concentra
Extrato de alho e óleo vegetal no controle do míldio da videira
Leite, Carla Daiane;Botelho, Renato Vasconcelos;Faria, Cacilda Marcia Duarte Rios;Maia, Aline José;
Revista Brasileira de Fruticultura , 2011, DOI: 10.1590/S0100-29452011000200013
Abstract: the objective of this study was to verify the effect of garlic extract and vegetable oil in the control of downy mildew cv. isabel (vitis labrusca). disease severity expressed as area under disease progress curve (audpc) and germination of the sporangia of its causal agent plasmopara viticola were evaluated. the treatments tested consisted of 0; 5, 10, 15, 20, 25 or 30 ml l-1 of garlic extract added to 2.5 ml l-1 of vegetable oil, bordeaux mixture (1:1:100) and control. mancozeb (2 g l-1) was used as a standard treatment in the germination test. in field conditions, there was reduction in severity of mildew with vegetable oil, and garlic extract from 20 ml l-1 potentiated such biocide action. the sporangium germination of p. viticola varied depending on the length of exposure to garlic extract, showing no efficient when compared to treatment with bordeaux mixture and mancozeb. vegetable oil had no effect on sporangium germination of this pathogen.
Efeito do extrato de alho na quebra de dormência de gemas de videiras e no controle in vitro do agente causal da antracnose (Elsinoe ampelina Shear)
Botelho, Renato Vasconcelos;Maia, Aline José;Pires, Erasmo José Paioli;Terra, Maurilo Monteiro;
Revista Brasileira de Fruticultura , 2009, DOI: 10.1590/S0100-29452009000100015
Abstract: considering the reduction or elimination of synthetic compounds used in sustainable fruit production systems, this work aimed to search for new alternatives for bud break dormancy and diseases control in grapevines. single-bud cuttings of grapevines were sprayed with the following treatments: 1) control, 2) 1% so (soybean oil), 3) 3% ge (garlic extract), 4) 3% ge + 1% so. after that, the cuttings were kept in a growth chamber (25±2.5oc) for 56 days. the unique treatment that stimulated sprouting of grapevines cv. isabel precoce was 3% ge + 1% s), that reached 35% sprouting, differing statistically from the control (12.5%), 1% so (17.5%) and 3% ge (15.0%). probably, the stage of deep endodormancy of buds, after only 90 chilling hours (< 7.0oc), avoided better results of bud breaks treatments. three experiments were carried out in vitro, with different garlic extract doses aiming to evaluate the control of the fungus elsinoe ampelina. in all trials, there were quadratic effects on mycelial growth, without differences between garlic extract treatments, evidencing its fungicide effect, even for the lowest dose of 0.0615% ge.
Efeitos de reguladores vegetais na propaga??o vegetativa do porta-enxerto de videira '43-43' (Vitis vinifera x V. rotundifolia)
Botelho, Renato Vasconcelos;Maia, Aline José;Pires, Erasmo José Paioli;Terra, Maurilo Monteiro;Schuck, Enio;
Revista Brasileira de Fruticultura , 2005, DOI: 10.1590/S0100-29452005000100004
Abstract: this work aimed to develop a methodology of vegetative propagation for the vine rootstock '43-43'. hardwood cuttings collected in august, softwood cuttings collected in january and semi-hardwood cuttings collected in march were immersed in solutions, by 10 minutes, containing different doses of the plant regulators paclobutrazol (0, 100 and 200 mg.l-1) and indole butyric acid (0, 500 and 1,000 mg.l-1), combined or not, totalizing nine treatments for each kind of cutting. sixty days after planting the cuttings, the following variables were evaluated: percentage of rooted cuttings, number of roots, fresh weight of roots and percentage of sprouted cuttings. the hardwood cuttings presented 100% of sprouted cuttings, but it was not verified rooting, independent of the utilization of plant regulators. the softwood cuttings that were not treated with plant regulators showed 92% of rooting and 84% of sprouting. for this king of cutting the iba at 1,000 mg.l-1 increased the number of roots. the semi-hardwood cuttings had the highest percentage of rooting (23.5%) when they were treated with iba at 1,000 mg.l-1. this treatment also propitiated the maximum fresh weight and number of roots, nevertheless, these values were inferior to those verified for softwood cuttings.
Estaquia do porta-enxerto de videira '43-43' (V. vinifera x V. rotundifolia) resistente à Eurhizococchus brasiliensis
Botelho, Renato Vasconcelos;Maia, Aline José;Pires, Erasmo José Paioli;Terra, Maurilo Monteiro;Schuck, Enio;
Revista Brasileira de Fruticultura , 2005, DOI: 10.1590/S0100-29452005000300033
Abstract: a trial was carried out aiming to evaluate the vegetative propagation by cutting for the vine rootstock '43-43'. softwood shoots collected in january - and semi-hardwood shoots collected in march - were divided in two lots: one of the cutting was taken from the apex and the other from the base. the cuttings were immersed in solutions, by 10 minutes, containing different doses of indole butyiric acid (0, 1000, 1500, 2000, 2500 or 3000 mgl-1), totalizing 12 treatments for each epoch of rooting. after that, the cuttings were planted in polypropylene vases with sand and kept under misty system irrigation. sixty days after planting the cuttings, for each epoch, the following variables were evaluated: percentage of rooted cuttings, length and fresh weight of roots and percentage of sprouted cuttings. the softwood and semi-hardwood cuttings presented high percentage of rooting (66% and 68%, respectively) even when not treated with the plant regulator. apical cuttings presented higher length and fresh weight of roots. the iba application reduced the percentage of rotting and sprouting of softwood and semi-hardwood cuttings. the semi-hardwood cuttings that were treated with iba at 1,000mgl-1 showed the highest length and the freshest weight of roots.
A??o de quitosana sobre o desenvolvimento de Plasmopara viticola e Elsinoe ampelina, in vitro e em videiras cv. Isabel
Maia, Aline José;Botelho, Renato Vasconcelos;Faria, Cacilda Márcia Duarte Rios;Leite, Carla Daiane;
Summa Phytopathologica , 2010, DOI: 10.1590/S0100-54052010000300003
Abstract: this work aimed to evaluate the effect of chitosan on the control of fungi elsinoe ampelina and plasmopara viticola, the causal agents of downy mildew and anthracnose in grapevines, respectively. the concentrations of 0, 20, 40, 80 and 160 mg l-1 chitosan were used in the following trials: mycelial growth, spore germination, and experiment in field conditions. for the last two trials standard treatments with mancozeb and bordeax mixture were added. there was a reduction in e. ampelina mycelial growth and the highest chitosan concentration (160 mg l-1) decreased by 57% the fungus development at 192 hours after incubation. in the germination tests, the concentration of 160 mg l-1 chitosan reduced spore germination in e. ampelina by approximately 98% and p. viticola by 60%, not differing from the treatments with bordeaux mixture and mancozeb. in the field trial the highest chitosan concentrations (80 and 160 mg l-1) decreased anthracnose severity between 93 and 81%. for downy mildew, the concentration of 160 mg l-1 decreased the disease by approximately 81%. based on these results, chitosan has a great potential for the control of downy mildew and anthracnose in grapevines.
Chitosan as an option to control mildew in the sustainable vinegrowing Quitosana como op o de controle do míldio para viticultura sustentável
Aline José Maia,Carla Daiane Leite,Renato Vasconcelos Botelho,Cacilda Márcia Duarte Rios Faria
Semina : Ciências Agrárias , 2012,
Abstract: The aim of this study was to evaluate different concentrations of chitosan on the severity of mildew and the germination of Plasmopara viticola, in addition to its effect on vegetative growth of grapevines cv. Cabernet Sauvignon and Merlot. Two experiments were conducted. In the first vine cuttings were planted in pots with Plantmax and sand 1:1 (v:v) and maintained in a greenhouse under intermittent mist. Every seven days were sprayed with aqueous solutions of chitosan at concentrations of 0, 20, 40, 80 and 160 mg L-1. The leaves were inoculated with sporangia of P. viticola 48 hours after the second application of chitosan. The first symptoms appeared ten days after inoculation, and severity ratings were performed every two days. The concentration of 160 mg L-1 of chitosan decreased the severity of downy mildew in 70.2 % and 79.1% in cv. Merlot and Cabernet Sauvignon, respectively. Sporangial germination tests were also conducted. The highest concentration of chitosan (160 mg L-1) inhibited by 50 % germination of P. viticola after 24 hours. In the second experiment, cuttings, pre-rooted, were obtained and handled as the previous experiment. After 60 days in a greenhouse, we evaluated the following variables: mass of roots and leaves, root length and leaf area. There was no significant difference between treatments for the variables related to plant development. O objetivo deste trabalho foi avaliar diferentes concentra es de quitosana na severidade do míldio e na germina o de Plasmopara viticola, além do seu efeito no desenvolvimento vegetativo das videiras cv. Cabernet Sauvignon e Merlot. Foram realizados dois experimentos. No primeiro, estacas de videira foram plantadas em vasos contendo substrato comercial Plantmax e a areia na propor o 1:1 (v:v) e mantidas em casa de vegeta o sob nebuliza o intermitente. A cada sete dias foram pulverizadas com solu es aquosas de quitosana nas concentra es de 0, 20, 40, 80 e 160 mg L-1. As folhas foram inoculadas com suspens o de esporangios de P. viticola 48 horas e após a segunda aplica o de quitosana. Os primeiros sintomas apareceram dez dias após a inocula o, e as avalia es da severidade foram realizadas a cada dois dias. A concentra o de 160 mg L-1 de quitosana reduziu a severidade do míldio em 70,2 % e 79,1 % nas cv. Merlot e Cabernet Sauvignon, respectivamente. Testes de germina o de esporangios também foram realizados. A maior concentra o de quitosana (160 mg L-1) inibiu em 50 % da germina o de P. viticola após 24 horas. No segundo experimento, as estacas, pré-enraizadas, foram obtidas e tra
Efeitos da quitosana no desenvolvimento in vitro de videiras cv. merlot e no crescimento micelial do fungo Elsinoe ampelina
Maia, Aline José;Leite, Carla Daiane;Botelho, Renato Vasconcelos;Faria, Cacilda Márcia Duarte Rios;Uber, Suelen Cristina;
Ciência e Agrotecnologia , 2010, DOI: 10.1590/S1413-70542010000600010
Abstract: the objective of this research was to evaluate the effects of the chitosan on the development in vitro plantlets of grapevines cv. merlot and its antifungal action on elsinoe ampelina. in the first trial, explants of the grapevine cv. merlot were transferred to growing medium dsd1 plus concentrations of 0; 25; 50; 100; 150 and 200 mg l-1 of the product. after 90 days of in vitro cultivation the plantlets were evaluated for root and leaf number, root and shoot length and fresh mass of roots and shoots. in the second experiment, doses of 0; 60; 120; 180; 240 and 300 mg l-1 were incorporated to the bda media, where the fungus was inoculated. the mycelial diameter was evaluated at 6 and 9 days after incubation at 25° c in the dark. in the first experiment, for the variables shoot length average, plant fresh mass, rooting percentage and sprouted cuttings there was a linear reduction in function of chitosan doses. in the second trial, there was a negative linear effect with mycelial growth inhibition, of 81.7%, showing great the potential of chitosan in the control of grapevine anthracnose.
Efeito do extrato de alho na quebra de dormência de macieiras
Botelho, Renato Vasconcelos;
Revista Brasileira de Fruticultura , 2007, DOI: 10.1590/S0100-29452007000200042
Abstract: with the objective of evaluate the effect of garlic extract on dormancy breaking of apple trees, a trial was carried out in a commercial orchard of the royal gala and fuji kiku cultivars, located in guarapuava, middle-west region of paraná state, brazil. the treatments were as following: t1 - control; t2 - garlic extract, and t3 - 0.45% hydrogenated cyanamide + 3% mineral oil. the applications were accomplished on september 3rd, 2004, on phenological stage of dormant bud. the evaluations of sprouted buds percentage were carried out weekly, from september 17th to october 6th 2004. for both cultivars, the best results were verified for the treatment with hydrogenated cyanamide and mineral oil (71.3%). the treatment with garlic extract also promoted dormancy breaking of 'fuji kiku' apple trees, with 40.6% of sprouted buds after 35 days from the applications, compared to just 18.2% of the control.
Biochemical Characterization of an In-House Coccidioides Antigen: Perspectives for the Immunodiagnosis of Coccidioidomycosis
Renato Evando Moreira Filho,Silviane Praciano Bandeira,Raimunda Samia Nogueira Brillhante,Marcos Fábio Gadelha Rocha,Ilka Maria Vasconcelos,Mirella Leite Pereira,Débora de Souza Collares Maia Castelo-Branco,Francisco Airton Castro Rocha,Zoilo Pires de Camargo,Marcio Viana Ramos,Rossana de Aguiar Cordeiro,José Júlio Costa Sidrim
Molecules , 2012, DOI: 10.3390/molecules17077854
Abstract: The objective of this study was to evaluate the reactivity of an in-house antigen, extracted from a strain of C. posadasii isolated in northeastern Brazil, by radial immunodiffusion and Western blotting, as well as to establish its biochemical characterization. The protein antigen was initially extracted with the use of solid ammonium sulfate and characterized by 1-D electrophoresis. Subsequently, it was tested by means of double radial immunodiffusion and Western blotting. A positive reaction was observed against the antigen by both immunodiagnostic techniques tested on sera from patients suffering from coccidioidomycosis. Besides this, two immunoreactive protein bands were observed and were revealed to be a β-glucosidase and a glutamine synthetase after sequencing of the respective N-terminal regions. Our in-house Coccidioides antigen can be promising as a quick and low-cost diagnostic tool without the risk of direct manipulation of the microorganism.
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