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Search Results: 1 - 10 of 5062 matches for " Renata Walczak-Jedrzejowska "
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Blood lipids may have influence on the emotional well-being in young men  [PDF]
Edyta Kramek, Sylwia Jastrzebska, Renata Walczak-Jedrzejowska, Katarzyna Marchlewska, Elzbieta Oszukowska, Anna Guminska, Krzysztof Kula, Jolanta Slowikowska-Hilczer
Health (Health) , 2010, DOI: 10.4236/health.2010.25066
Abstract: Anamnestic data on general health and medical conditions were achieved from 136 men (20-49 yrs). Beck Depression Inventory II (BDI-II) questionnaire was used to assess depressive symptomatology. Body weight, height, waist and hip circumference, arterial blood pressure were mea- sured. Serum levels of total cholesterol (TC), LDL cholesterol (LDL-C), HDL cholesterol (HDL-C), triglicerydes (TG), glucose, SHBG, total testosterone, DHEA-S and estradiol were determined. Calculated were body mass index (BMI), waist to hip ratio (WHR) and free testosterone index (FTI). In men aged 40-49 general health significantly got worse, BMI, WHR, blood pressure increased and mean serum levels of FTI, DHEA-S, estradiol decreased in comparison to younger men. Only in 40-49 age band BDI-II scoring was negatively related with FTI, however, in the whole group there were no significant correlations. Nevertheless, some symptoms of depression were negatively related with LDL-C or HDL-C and positively with TG. Similar relations were found among young men, but not in the middle-aged. Conclusions: Only blood lipids may have influence on emotional well-being in young healthy men. The decreased testosterone level becomes probably the main risk factor for the lower mood in middle-aged men. Atherosclerosis risk factors and general health worsen with the advancing age, but they have no significant effect on psychological situation.
Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis.
Eliza Filipiak,Renata Walczak-Jedrzejowska,Elzbieta Oszukowska,Anna Guminska
Folia Histochemica et Cytobiologica , 2010, DOI: 10.5603/4288
Abstract: The aim of this study was to assess the impact of xenoestrogens: diethylstilbestrol (DES) and zearalenone (ZEA) on rat's pubertal testis and to compare it with the effect of natural estrogen - 17beta-estradiol (E). Male Wistar rats were daily, subcutaneously injected at 5th-15th postnatal days (p.d.) with E (1.25 or 12.5 mug) or DES (1.25 or 12.5 mug) or ZEA (4 or 40 mug) or vehicle. At 16th p.d. testes were dissected, weighted, and paraffin embedded. Following parameters were assessed: diameter and length of seminiferous tubule, numbers of spermatogonia A+intermediate+B (A/In/B), preleptotene spermatocytes (PL), leptotene+zygotene+pachytene spermatocytes (L/Z/PA) and Sertoli cells per testis. Testes weight, seminiferous tubule diameter and length were decreased by both doses of E, DES and ZEA. DES effect was the strongest, but its influence on testis weight and seminiferous tubule length, on the contrary to E and ZEA, was not dose-dependent. Similarly, DES in both doses had the most severe negative impact on the number of germ and Sertoli cells. The negative influence of E on germ cells was less pronounced. The negative effect of ZEA was seen only after administration of the higher dose on spermatogonia number, while DES and E decreased A/In/B number more evidently. Sertoli cell number were decreased after both doses of E. ZEA40 decreased Sertoli cell number while ZEA4 had no effect. Conclusion: exposure of prepubertal male rat to DES has the strongest detrimental effect on the developing testis in comparison to E and ZEA. Both, E and DES, decreased number of germ and Sertoli cells, diminished seminiferous tubule diameter, length and testis weight. ZEA had much more weaker effect than the potent estrogens.
Estrogen receptor alpha localization in the testes of men with normal spermatogenesis Estrogen receptor alpha localization in the testes of men with normal spermatogenesis
Eliza Filipiak,Dagmara Suliborska,Maria Laszczynska,Renata Walczak-Jedrzejowska
Folia Histochemica et Cytobiologica , 2012, DOI: 10.5603/19743
Abstract: It is known that estrogens act on the male reproductive tract by binding to estrogen receptors (ER) a and b. However, studies on ER localization in the human testis are discordant. The aim of this study was to investigate the localization of ERa in the testes of adult men with normal spermatogenesis. Semen analysis of ten adult men revealed azoospermia. FSH, LH and testosterone serum concentrations were within normal values, and the volume of the testes was normal, hence obstructive azoospermia was suspected. The tissues from testicular surgical biopsies were fixed in Bouin’s fluid and embedded in paraffin. Assessments of the seminiferous epithelium (scoring 10 to –1), the number of Leydig cells (scoring 1 to 5), the areal fraction of intertubular space (IS), measurements of seminiferous tubule diameter, and the thickness of the tubular wall, were performed on microscopic sections. Immunohistochemical staining was applied with monoclonal antibodies against ERa. The mean spermatogenesis score was 10 points; IS — 30.6 ± 8.1%; seminiferous tubule diameter — 193.9 ± 19.4 μm; thickness of tubular wall — 7.44 ± 1.1 μm; number of Leydig cells — 1.6 ± 1.1 points. Immunohistochemical staining showed the localization of ERa to be in the Sertoli and Leydig cell cytoplasm, while ERa was absent in germ cells. The results of testicular tissue analysis confirmed its normal structure and normal, full spermatogenesis. The presence of ERa in Sertoli and Leydig cells in normal human testis demonstrated in this study suggests that estrogens may affect testicular function. It is known that estrogens act on the male reproductive tract by binding to estrogen receptors (ER) a and b. However, studies on ER localization in the human testis are discordant. The aim of this study was to investigate the localization of ERa in the testes of adult men with normal spermatogenesis. Semen analysis of ten adult men revealed azoospermia. FSH, LH and testosterone serum concentrations were within normal values, and the volume of the testes was normal, hence obstructive azoospermia was suspected. The tissues from testicular surgical biopsies were fixed in Bouin’s fluid and embedded in paraffin. Assessments of the seminiferous epithelium (scoring 10 to –1), the number of Leydig cells (scoring 1 to 5), the areal fraction of intertubular space (IS), measurements of seminiferous tubule diameter, and the thickness of the tubular wall, were performed on microscopic sections. Immunohistochemical staining was applied with monoclonal antibodies against ERa. The mean spermatogenesis score was 10 points
Endogenous estradiol and testosterone may predispose toward atherogenic lipid profile, but higher blood level of testosterone is associated with lower number of stenoses in the coronary arteries of men with coronary disease
Jerzy Krzysztof Wranicz,Iwona Cygankiewicz,Piotr Kula,Renata Walczak-Jedrzejowska
International Journal of Biomedical Science , 2006,
Abstract: Objectives: To assess the correlations between blood levels of sex steroid hormones and blood lipid profile or the degree of coronary artery stenosis in men with coronary artery disease (CAD). Methods: 111 men with stable CAD, aged 36-73 yrs, unselected for the coexisting clinical coronary risk factors were prospectively studied. Degree of coronary stenosis was assessed angiographically using different indices. Total cholesterol (T-Ch), high density lipoproteins cholesterol (HDL-Ch), low density lipoproteins cholesterol (LDL-Ch), triglicerydes (TG), testosterone, estradiol, dehydroepiandrosterone sulfate (DHEA-S), and sex hormone binding globulin (SHBG) were measured in the blood. Free testosterone index (FTI) was calculated. Results: A positive, significant correlations were found between blood concentrations of estradiol and T-Ch (r=0.29, p< 0.01) or LDL-Ch (r=0.34, p<0.005) as well as between FTI and blood LDL-Ch (r=0.23, p< 0.05). Blood level of estradiol negatively correlated with HDL-Ch/T-Ch ratio (r= -0.21, p<0.05). While blood levels of T-Ch correlated positively with 3 out of 5 applied here indices of coronary stenosis, blood LDL-Ch with two of them. In turn, blood level of testosterone negatively correlated with one index of coronary stenosis (r= -0.26, p<0.05). Conclusion: In men with CAD, plasma estradiol concentrations are predictive for T-Ch, LDL-Ch and HDL-Ch/TCh ratio, and FTI for LDL-Ch. Regression analyses indicated that while sex steroid hormones may predispose toward atherogenic lipid profile and are predictive for the number and degree of coronary artery stenosis, higher blood level of total testosterone was associated with the lower number of stenosis in the coronary arteries. Hence, endogenous testosterone may have beneficial effect on coronary arteries.
Administration of testosterone inhibits initiation of seminal tubule growth and decreases Sertoli cell number in the earliest period of rat's postnatal development.
Renata Walczak-Jedrzejowska,Jolanta S?owikowska-Hilczer,Katarzyna Marchlewska,Elzbieta Oszukowska
Folia Histochemica et Cytobiologica , 2010, DOI: 10.5603/4265
Abstract: Sertoli cell (SC) number determines testes size and their capacity to produce spermatozoa. In the rat SC proliferate until 15th postnatal day (PND). Their proliferation is stimulated by FSH and inhibited by estradiol, but the role for androgens is uncertain. In this study we analyzed the effects of testosterone administration on testes growth and SC number in relation to timing of the treatment. Male rats were injected with 2.5 mg of testosterone propionate (TP) from birth until 5th PND and autopsied either on 6th PND [TP1-5(6)] or on 16th PND [TP1-5(16)] (transient administration). Other rats received TP from birth until 15th PND [TP1-15] or between 5th and 15th PND [TP5-15] continuously and were autopsied on day 16th. Control groups (C) received vehicle. In the Cs serum level of estradiol was 20-fold higher (p<0.001) and FSH was 1,7-fold higher (p<0.05) on 6th PND than on 16th PND, while testosterone did not change. After TP blood level of testosterone increased 2200-fold on 6th PND (p<0.05), and 8-fold on 16th PND. In turn, continuous TP administrations resulted on 16th PND in the increase in testosterone serum level by 2000-times of C without influence on FSH. While the treatment from birth either during initial 5 days or continuously until 15th day decreased testicular weight (p<0.001), tubule length (p<0.05) and SC number (p<0.001), the treatment initiated on 5th PND had no effects. TP reduced serum estradiol level on 6th PND by 13-fold (p<0.01), but doubled it on 16th PND. Conclusion: Neonatal rats secrete estradiol and FSH in the amounts greatly extending those presented during further development. Testosterone inhibits testicular growth and SC number acting during first 5 neonatal days by decreasing FSH secretion, but is not effective during further development. Direct inhibitory influence of testosterone or trough its increased aromatisation to estradiol beyond neonatal period may be responsible for sustained inhibition of testes growth and SC number during infancy.
During seminiferous tubule maturation testosterone and synergistic action of FSH with estradiol support germ cell survival while estradiol alone has pro-apoptotic effect.
Renata Walczak-Jedrzejowska,Jolanta Slowikowska-Hilczer,Katarzyna Marchlewsk,Elzbieta Oszukowska
Folia Histochemica et Cytobiologica , 2008, DOI: 10.5603/4489
Abstract: During establishment of spermatogenesis at the prepubertal age, an early germ cells apoptotic wave occurs likely aimed to remove abnormal germ cells and to maintain a proper cell number ratio between maturating germ cells and Sertoli cells. Here we assessed Sertoli and germ cell apoptosis in relation to morphological parameters of Sertoli cell maturation in neonatal rats under the influence of testosterone, estradiol and FSH given alone or in combinations. From postnatal day (PND) 5th to 15th male rats were daily injected with: 1) 2.5 mg of testosterone propionate (TP), or 2) 12.5 microg of 17beta-estradiol benzoate (EB), or 3) TP+EB, or 4) 7.5 IU of human purified FSH (hFSH), or 5) hFSH+EB or solvents (control-C). Autopsy was performed on PND 16th. Sertoli cell nuclei area and incidence of seminiferous tubule lumen formation (LF) were taken as markers of Sertoli cell maturation. Sertoli and germ cell apoptosis was assessed using TUNEL method. In comparison with C, the area of Sertoli cell nuclei was significantly reduced after EB (25.7+/-2.0 vs. 30.9+/-1.6 microm2 for C, p<0.001) and increased after hFSH+EB (33.1+/-2.3 microm2, p<0.05). Incidence of LF was completely arrested by steroid hormone treatments given separately, significantly inhibited after TP+EB (median: 0.0%, vs. 2.0% for C p<0.05) and significantly enhanced after hFSH+EB (median: 51.0%, p<0.001). hFSH alone did not influence LF. Incidence of TUNEL positive Sertoli cells significantly increased after EB (median: 2.9% vs. 0.5% for C, p<0.05) or TP+EB (median: 2.2%, p<0.01) and was not affected by other treatments. Incidence of TUNEL positive germ cells increased significantly after EB alone (median: 4.4% vs. 2.5%, for C, p<0.01 ) and was significantly decreased by hFSH+EB (median: 0.5%, p<0.01). CONCLUSIONS: 1) Administration of testosterone or estradiol to immature rats inhibits Sertoli cell maturation. 2) Estradiol stimulates Sertoli and germ cell apoptosis while testosterone has no effect. 3) Testosterone eliminates estradiol--induced germ cell apoptosis when both hormones act in concert. 4) FSH in concert with estradiol, but neither one of the hormone alone, accelerate Sertoli cell differentiation and effectively inhibit germ cell apoptosis. 5) During seminiferous tubule maturation testosterone and the synergistic action of FSH with estradiol support germ cell survival while estradiol alone has an inhibitory, pro-apoptotic effect.
During seminiferous tubule maturation testosterone and synergistic action of FSH with estradiol support germ cell survival while estradiol alone has pro-apoptotic effect.
Renata Walczak-Jedrzejowska,Jolanta Slowikowska-Hilczer,Katarzyna Marchlewsk,Elzbieta Oszukowska
Folia Histochemica et Cytobiologica , 2008, DOI: 10.2478/4489
Abstract: During establishment of spermatogenesis at the prepubertal age, an early germ cells apoptotic wave occurs likely aimed to remove abnormal germ cells and to maintain a proper cell number ratio between maturating germ cells and Sertoli cells. Here we assessed Sertoli and germ cell apoptosis in relation to morphological parameters of Sertoli cell maturation in neonatal rats under the influence of testosterone, estradiol and FSH given alone or in combinations. From postnatal day (PND) 5th to 15th male rats were daily injected with: 1) 2.5 mg of testosterone propionate (TP), or 2) 12.5 microg of 17beta-estradiol benzoate (EB), or 3) TP+EB, or 4) 7.5 IU of human purified FSH (hFSH), or 5) hFSH+EB or solvents (control-C). Autopsy was performed on PND 16th. Sertoli cell nuclei area and incidence of seminiferous tubule lumen formation (LF) were taken as markers of Sertoli cell maturation. Sertoli and germ cell apoptosis was assessed using TUNEL method. In comparison with C, the area of Sertoli cell nuclei was significantly reduced after EB (25.7+/-2.0 vs. 30.9+/-1.6 microm2 for C, p<0.001) and increased after hFSH+EB (33.1+/-2.3 microm2, p<0.05). Incidence of LF was completely arrested by steroid hormone treatments given separately, significantly inhibited after TP+EB (median: 0.0%, vs. 2.0% for C p<0.05) and significantly enhanced after hFSH+EB (median: 51.0%, p<0.001). hFSH alone did not influence LF. Incidence of TUNEL positive Sertoli cells significantly increased after EB (median: 2.9% vs. 0.5% for C, p<0.05) or TP+EB (median: 2.2%, p<0.01) and was not affected by other treatments. Incidence of TUNEL positive germ cells increased significantly after EB alone (median: 4.4% vs. 2.5%, for C, p<0.01 ) and was significantly decreased by hFSH+EB (median: 0.5%, p<0.01). CONCLUSIONS: 1) Administration of testosterone or estradiol to immature rats inhibits Sertoli cell maturation. 2) Estradiol stimulates Sertoli and germ cell apoptosis while testosterone has no effect. 3) Testosterone eliminates estradiol--induced germ cell apoptosis when both hormones act in concert. 4) FSH in concert with estradiol, but neither one of the hormone alone, accelerate Sertoli cell differentiation and effectively inhibit germ cell apoptosis. 5) During seminiferous tubule maturation testosterone and the synergistic action of FSH with estradiol support germ cell survival while estradiol alone has an inhibitory, pro-apoptotic effect.
Review paper Cardiovascular and metabolic effects of estrogen in men
Jerzy Krzysztof Wranicz,Iwona Cygankiewicz,Piotr Kula,Renata Walczak-J?drzejowska
Archives of Medical Science , 2006,
Abstract: Although in men with an inherited mutation of gene encoding ERa (estrogen resistance) the occurrence of premature coronary artery disease was documented, and in men with inherited lack of aromatase (estrogen deficiency) an unfavorable lipid milieu was reported, the predominant number of both epidemiological and interventional studies suggests that in men estrogens may either not influence or may promote the develpoment of coronary artery disease. It is possible therefore the beneficial effect of estrogen administration on the lipid milieu in patients with estrogen deficiency is limited only to this unique situation. There may exist a sex-specific difference in the response to estrogen action. In contrast to women where estrogens generate nitric oxide (NO) production in the vascular endothelium, they do not do so in men. NO is responsible for vascular dilation and inhibits lipoprotein oxydation and monocyte adhesion to the endothelium. There may exist also a difference between short-term, non-genomic effect of estrogen and the effects of a long-term exposition to the hormone. Several reports are available indicating that estrogen administartion may have an unfavourable effect not only on blood lipid profile but also on venous thrombo-embolism in both sexes. In this context the role of estrogens in the regulation of the cardiovascular system gains a special importance and needs further studies.
Di(n-butyl) phthalate has no effect on the rat prepubertal testis despite its estrogenic activity in vitro
Eliza Filipiak,Renata Walczak-J?drzejowska,Mariusz Krupiński,El?bieta Oszukowska
Folia Histochemica et Cytobiologica , 2012, DOI: 10.5603/14730
Abstract: The aim of this study was to assess the impact of di(n-butyl) phthalate (DBP) on the rat’s prepubertal testis. Male Wistar rats were given daily subcutaneous injections with DBP (20 or 200 μg) or a vehicle from the 5th to the 15th postnatal day (pd). On the 16th pd, the rats were euthanized, and the testes were dissected, weighed, and paraffin embedded. The blood was collected to determine the serum levels of testosterone (T), estradiol (E) and FSH. The following parameters were assessed in the testis sections: diameter and length of seminiferous tubules (st), numbers of spermatogonia A + intermediate + B (A/In/B), preleptotene spermatocytes (PL), leptotene + zygotene + pachytene spermatocytes (L/Z/PA) and Sertoli cells per testis, percentage of st containing gonocytes or pachytene spermatocytes or lumen. An estrogenicity in vitro test was performed by means of a transgenic yeast strain expressing human estrogen receptor alpha. At both doses, DBP had no influence on testis and seminal vesicle weight, st diameter and length, number of germ and Sertoli cells per testis, percentage of st containing gonocytes or pachytene spermatocytes or lumen. DBP did not change E, T or FSH serum levels. The in vitro yeast screen showed that DBP was a weak estrogenic compound, approximately six to seven orders of magnitude less potent than 17β-estradiol. In conclusion, exposure of a rat to DBP in doses 100 or 1,000-fold higher than a Tolerable Daily Intake for humans had no effect on its testicular development. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 4, pp. 685–689)
De Sitter Space as a Computational Tool for Surfaces and Foliations  [PDF]
Maciej Czarnecki, Szymon Walczak
American Journal of Computational Mathematics (AJCM) , 2013, DOI: 10.4236/ajcm.2013.31A001
Abstract:

The set of all spheres and hyperplanes in the Euclidean space Rn+1 could be identified with the Sitter space Λn+1. All the conformal properties are invariant by the Lorentz form which is natural pseudo-Riemannian metric on Λn+1. We shall study behaviour of some surfaces and foliations as their families using computation in the de Sitter space.

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