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Search Results: 1 - 10 of 201446 matches for " Reginaldo P. Brazil "
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Isolation and Identification of 9-methylgermacrene-B as the Putative Sex Pheromone of Lutzomyia cruzi (Mangabeira, 1938) (Diptera: Psychodidae)
Brazil, Reginaldo P;Hamilton, JGC;
Memórias do Instituto Oswaldo Cruz , 2002, DOI: 10.1590/S0074-02762002000300030
Abstract: lutzomyia (lutzomyia) cruzi has been named as a probable vector of leishmania chagasi in corumbá, mato grosso do sul, brazil. taxonomically l. cruzi is closely related to the l. longipalpis species complex. females of l. cruzi and l. longipalpis are morphologically indistinguishable and associated males must be examined carefully to confirm identifications. chemical analysis hexane extracts of male l. cruzi has revealed the presence of a 9-methylgermacrene-b (c16), a homosesquiterpene (mw 218) previously shown to be the sex pheromone of one of the members of the l. longipalpis species complex.
Isolation and Identification of 9-methylgermacrene-B as the Putative Sex Pheromone of Lutzomyia cruzi (Mangabeira, 1938) (Diptera: Psychodidae)
Brazil Reginaldo P,Hamilton JGC
Memórias do Instituto Oswaldo Cruz , 2002,
Abstract: Lutzomyia (Lutzomyia) cruzi has been named as a probable vector of Leishmania chagasi in Corumbá, Mato Grosso do Sul, Brazil. Taxonomically L. cruzi is closely related to the L. longipalpis species complex. Females of L. cruzi and L. longipalpis are morphologically indistinguishable and associated males must be examined carefully to confirm identifications. Chemical analysis hexane extracts of male L. cruzi has revealed the presence of a 9-methylgermacrene-B (C16), a homosesquiterpene (mw 218) previously shown to be the sex pheromone of one of the members of the L. longipalpis species complex.
Identification of the sex pheromone of Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae) from Asunción, Paraguay
Reginaldo P Brazil, Norath Caballero, James Hamilton
Parasites & Vectors , 2009, DOI: 10.1186/1756-3305-2-51
Abstract: In this study, we present the results of a coupled gas chromatography - mass spectrometry analysis of the pheromones of males Lu. longipalpis captured in an endemic area of visceral leishmaniasis in Asunción, Paraguay. Our results show that Lu. longipalpis from this site produce (S)-9-methylgermacrene-B which has also been found in Lu. longipalpis from different areas of Brazil, Colombia and Central America.Visceral leishmaniasis is endemic in several areas of Paraguay with the reports of sporadic cases and consecutive increase in the last few years [1-3]. Asuncion in the Central Department of Paraguay has reported the largest numbers of human cases but other regions more distant from the capital, such as Bella Vista Norte, near the border with Brazil, Encarnacion, near the border with Argentina, and the Departments of Conception and Amambay y San Pedro have been considered as new endemic areas by the Paraguayan Health Secretary [4,5].As in most endemic areas, Lutzomyia longipalpis s.l. is the main vector of Leishmania (L.) infantum (Kinetoplastida: Trypanosomatidae), the causative agent of visceral leishmaniasis in South and Central America. Even though Lu. longipalpis is recognized as a species complex, no consensus has been established on the number of species present in the New World [6-13].Field and laboratory observations have shown that, prior to copulation, Lu. longipalpis s.l. males wing flutter. This behaviour is associated with pheromone release by males during courtship [14-16]. The sex pheromones are produced in glandular tissue that underlies the cuticle of the abdominal tergites. Those pheromone-disseminating structures are visible, as a pair of pale patches [17,18] on the fourth or third and fourth tergites and have been confirmed as the site of sex pheromone production [19]. There is no relationship between spot morphology and sex pheromone type [20]. Sex pheromones of the Lu. longipalpis species complex have been shown to be homosesquiterpenes (C16
Phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) of Alagoas state, northeast of Brazil
Andrade Filho, José D;Brazil, Reginaldo P;
Neotropical Entomology , 2009, DOI: 10.1590/S1519-566X2009000500022
Abstract: the phlebotomine sandflies of the state of alagoas are poorly known, with more than 40 years since the last report on sandflies in the state. in here, psathyromyia brasiliensis (costa lima), micropygomyia quinquefer (dyar,) and evandromyia termitophila (martins, falc?o & silva) are registered for the first time in alagoas. this report increases to nine the number of species collected in the state, including lutzomyia longipalpis (lutz & neiva), migonemyia migonei (fran?a), nyssomyia whitmani (antunes & coutinho) and nyssomyia intermedia (lutz & neiva), all vectors of leishmania in brazil.
Leishmania (Viannia) lainsoni (Kinetoplastida: Trypanosomatidae), a divergent Leishmania of the Viannia subgenus: a mini review
Corrêa, José R;Brazil, Reginaldo P;Soares, Maurilio J;
Memórias do Instituto Oswaldo Cruz , 2005, DOI: 10.1590/S0074-02762005000600014
Abstract: leishmania (viannia) lainsoni is the leishmania species that presents the most distinct biological (morphology, growth in axenic culture medium), biochemical (enzymatic electrophoresis profile), and molecular biology characteristics, when compared to other species of the viannia subgenus. development of promastigote forms of this parasite attached to the wall of the pyloric and hind gut regions of sand fly vectors is a solid characteristic that allows its positioning in the viannia subgenus. however, taxonomic data from biochemical and molecular techniques on this leishmania species are still not conclusive. it is evident the difficulty in taxonomically positioning this borderline leishmania species. in this review we present the data accumulated since l. (viannia) lainsoni has been described and we discuss its position in the viannia subgenus.
Synthetic sex pheromone attracts the leishmaniasis vector Lutzomyia longipalpis to experimental chicken sheds treated with insecticide
Daniel P Bray, Graziella B Alves, Maria E Dorval, Reginaldo P Brazil, J GC Hamilton
Parasites & Vectors , 2010, DOI: 10.1186/1756-3305-3-16
Abstract: Addition of synthetic pheromone resulted in greater numbers of male and female sand flies being caught and killed at experimental chicken sheds sprayed with insecticide, compared to pheromone-less controls. Furthermore, a ten-fold increase in the amount of sex pheromone released from test sheds increased the number of females attracted and subsequently killed. Treating sheds with insecticide alone resulted in a significant decrease in numbers of males attracted to sheds (compared to pre-spraying levels), and a near significant decrease in numbers of females. However, this effect was reversed through addition of synthetic pheromone at the time of insecticide spraying, leading to an increase in number of flies attracted post-treatment.In field trials of commercially available different coloured sticky traps, yellow traps caught more males than blue traps when placed in chicken sheds. In addition, yellow traps fitted with 10 pheromone lures caught significantly more males than pheromone-less controls. However, while female sand flies showed a preference for both blue and yellow pheromone traps sticky traps over white traps in the laboratory, neither colour caught significant numbers of females in chicken sheds, either with or without pheromone.We conclude that synthetic pheromone could currently be most effectively deployed for sand fly control through combination with existing insecticide spraying regimes. Development of a standalone pheromone trap remains a possibility, but such devices may require an additional attractive host odour component to be fully effective.The sand fly Lutzomyia longipalpis (Diptera: Psychodidae) is the principle vector of Leishmania infantum chagasi (Kinetoplastida: Trypanosomatidiae), the causative agent of American visceral leishmaniasis (AVL) in Brazil and South America. Transmission occurs through bloodfeeding of female L. longipalpis on infected hosts [1], with domestic dogs the primary reservoir in urban and peri-urban areas. Treatmen
Description of evandromyia spelunca, a new phlebotomine species of the cortelezzii complex, from a cave in Minas Gerais State, Brazil (Diptera: Psychodidae: Phlebotominae)
Gustavo ML Carvalho, Reginaldo P Brazil, Cristiani C Sanguinette, José D Andrade Filho
Parasites & Vectors , 2011, DOI: 10.1186/1756-3305-4-158
Abstract: The morphological characters of the new species permit to include in the Evandromyia genus, cortelezzii complex. This complex consists of three species: Evandromyia corumbaensis (Galati, Nunes, Oshiro & Rego, 1989), Evandromyia cortelezzii (Brethes, 1923) and Evandromyia sallesi (Galvao & Coutinho, 1940).The new species can be separate from the others of the cortelezzii complex through morphological characters of the male terminalia and female spermathecae.Sand flies are responsible for transmission of the genus Leishmania among vertebrates hosts and study of this insect group is of great importance in attempts to control of leishmaniasis [1]. In Brazil, the cave fauna of insects is poorly documented, and among the insects that live or frequent caves and their adjacent environments, phlebotomine sand flies call for special attention because several species are vectors of arboviruses and protozoa, among other parasites.The geographical distribution of the sand flies species depends on their ability to adapt to different ecological niches. Thus, by its development in the immature stages and their feeding habits when adults, the species of sand flies are found where both larvae and adults can find appropriate environments for their development, including blood supply for females. In this way, the geographical distribution of species can be restricted to their access to a specific environment and vertebrate host. Already the occurrence of leishmaniasis is basically determined by the presence of both a susceptible vector and a host/reservoir equally susceptible to the infection [2].The growing of tourism, involving a search for natural attractions such as cave exploration, demands a better knowledge of the threats to health that people may face in those new areas.In the present investigation a new species of sand fly from Minas Gerais is described based on both genders collected in a cave in the Lassance municipality.Sand flies were collected using CDC light traps (HP mo
Natural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazil
Jo?o CA Carreira, Alba V Machado da Silva, Daniela de Pita Pereira, Reginaldo P Brazil
Parasites & Vectors , 2012, DOI: 10.1186/1756-3305-5-111
Abstract: The opossums studied were caught by wire traps (Tomahawk) in Barra de Guaratiba, a peri-urban area in Rio de Janeiro. The opossums were killed with an overdose of Thiopental sodium.At necropsy, macroscopic alterations were examined and samples from liver, spleen, lymph nodes, ear, abdominal skin, scent glands and bone marrow were collected for parasitological and molecular diagnoses.Forty-eight opossums were captured in an AVL endemic region, 30 being caught in a mangrove area and eighteen animals in a forest area near to some residential-yards. Among the thirty opossums trapped in the mangrove area, all of them were negative by both imprint and sera samples assayed on Dipstick Tests, that is a test based on a combination of protein-A colloidal gold conjugate and rk39 Leishmania antigen to detect anti-Leishmania antibody in serum or plasma. At the macroscopic examination one out of eighteen opossums, caught close to the forest, presented alterations compatible with spleen hypertrophy and three were positive by Dipstick Tests (16.6%) and presented amastigotes in the spleen and in one of them, the parasites were also observed in a submandibular lymph node. Leishmania infantum infections were confirmed through dot blot hybridization using a L. infantum-specific biotinylated probe.In the present paper we present the first report of amastigotes in the tissues of Didelphis aurita (Mammalia: Marsupialia) naturally infected with Leishmania infantum. We also attempt to claim the particular role of some opossum species as hosts of Leishmania infantum, contributing at least in part on the description of potential sylvatic reservoirs.
Distribui??o geográfica do complexo cortelezzii (Diptera: Psychodidae: Phlebotominae) no Brasil
Carvalho, Gustavo M L;Brazil, Reginaldo P;Falc?o, Alda L;Andrade Filho, José D;
Neotropical Entomology , 2009, DOI: 10.1590/S1519-566X2009000600026
Abstract: there are nearly 500 species of sandflies in americas, 30 of which are vectors of leishmaniasis. evandromyia cortelezzii (brèthes), e. sallesi (galv?o & coutinho) and e. corumbaensis (galati et al) comprise the cortelezzii complex. these species are morphologically similar and many times misidentified. due to the epidemiological importance recently attributed to this complex, a detailed study was carried out in order to correct wrongful information due to their misidentification, and update their geographical distribution. evandromyia corumbaensis was found to have a regional distribution, while the other two species are widely distributed in the brazilian territory.
Nota sobre Lutzomyia mamedei Oliveira, Afonso, Dias & Brazil (Diptera: Psychodidae)
Andrade Filho, José D.;Falc?o, Alda L.;Brazil, Reginaldo P.;
Anais da Sociedade Entomológica do Brasil , 1999, DOI: 10.1590/S0301-80591999000200023
Abstract: after examing the holotype and paratypes of lutzomyia mamedei kept at the sandfly collection at centro de pesquisas rené rachou-fiocruz we observed small posterior projections of the ascoids and very small spines in the hind femurs. although l. mamedei had been allocated in the subgenus pressatia, both characters are typical of the subgenus pintomyia costa lima 1932 thus we reallocate l. mamedei in this subgenus.
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