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Transcriptome Profiles and Gene Expression of Min6 Cells Are Altered by Pancreatic Stellate Cells  [PDF]
Ratnakar Reddy Bynigeri, Sasikala Mitnala, Ravikanth Vishnubhotla, Rupjyoti Talukdar, Surya Satyanarayana Singh, Nageshwar Reddy Duvvuru
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.96018

Aim: To identify the influence of pancreatic stellate cell (PSCs) secretions on gene expression profiles of Min6 cells by whole transcriptome sequencing. Methods: Pancreatic stellate cells (PSCs) were isolated from C57BL6J mice and propagated in vitro to acquire the activated phenotype. Total RNA was isolated from monocultured (MC) and PSC cocultured (CC) Min6 cells to prepare cDNA libraries, which were subjected to whole transcriptome sequencing for identifying differential expression of β-cell transcription factors (Pdx-1, Rfx6 and NeuroD1) related to insulin gene transcription and GSIS related genes such as Glut2, Gck, Abcc8, Kcnj11 and L-type Ca2+ channels (Cacnb2, Cacna1c). qRT-PCR was used to validate the gene expression. GSIS of Min6 cells was examined by estimating insulin

Haematological Profile and Growth Performance of Goats under Transportation Stress
Veterinary World , 2009,
Abstract: Stress in caprine lowers overall growth, performance and induces various haematological alterations. A study was conducted in 24 Osmanabadi goats divided randomly into four groups; negative control (I), positive control (II), treated groups III & IV. Group III was given Restobal liquid@20 ml/animal bid & group IV, Stresomix premix@5gm/day/animal with gur for 10 days. Group II, III, IV were subjected to transportation stress on day 10th. Pre-load and post-load transported goats were weighed to assess the weight loss during the journey and blood samples were collected for haematological evaluation. Administration of both the antistressor, immunomodulator and performance enhancer formulations to goats prior to their transportation was found safe and efficacious as evident by significantly lesser shrinkage of body weight and normalization of physiological and haematological parameters. [Vet. World 2009; 2(5.000): 195-198]
Commensal E. Coli Strains Uniquely Alter the ECM Topography Independent of Colonic Epithelial Cells  [PDF]
Shruthi S. Bharadwaj, Victor Nekrasov, Ramana Vishnubhotla, Crystal Foster, Sarah C. Glover
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2012, DOI: 10.4236/jbnb.2012.31009
Abstract: The relationship between commensal bacteria and the epithelial cells lining the colon is normally symbiotic. However, in the setting of diseases which lead to a loss of the protective mucosal layer such as inflammatory bowel disease or colon cancer, commensal bacteria gain the ability to alter both the behavior of epithelial cells as well as their surrounding extra cellular matrix (ECM). While much work has been done to understand the effects of bacteria on diseased epithelial cells in the colon, very little has been done to understand their impact on the ECM. In our previous work, we have shown that topographical changes in the ECM on the luminal side of the colon have a profound influence on the behavior of colonic epithelial cells. However, we do not understand all of the mechanisms that lead to changes in the ECM topography. This study aimed to understand the role that commensal E. coli strains play in altering the ECM topography of type-1 collagen scaffolds. To do this, 1.2 mg/ml type 1 collagen scaffolds were infected with various commensal bacterial strains. At 24 hours post-infection collagen fiber dimensions and substrate topography were determined using standard molecular biology techniques and advanced imaging. Intriguingly, all of the commensal E. coli strains showed some form of substrate degradation. Especially in the case of commensal E. coli strain HS4, maximum nano-scaled protrusions were observed. This data suggests, for the first time, that studying the effects of bacteria alone on the ECM may be critical to improving our understanding of how the cellular microenvironment changes in both health and disease.
Influence of calcium and phosphorus supplements with synergistic herbs on egg shell quality in late layers
Veterinary World , 2009,
Abstract: A study was conducted in 55 week old laying hen (n=225) to determine efficacy of calcium and phosphorus supplements in improving egg and shell quality traits. Birds were randomly divided into three groups, one control and two treatments. Group I (control) was offered basal diet without any additional supplement source of Calcium and phosphorus. Group II was supplemented Ayucal liquid (Dabur Ayurvet Ltd.) @15ml/ 100 birds/ day in drinking water and group III administered Ayucal premix@15g/ 100 birds/ day in feed from 55th- 62nd week age. Parameters studied were egg weight, shell thickness, breaking strength, number of shell defects and specific gravity of egg. Results of experiemental study revealed that supplementation of Ayucal liquid & Ayucal premix (supplied by Ayurvet Ltd. Baddi, India) improved overall egg and shell quality traits that can be correlated well with the activity of herbal constituents of the products owing calcium and phosphorus mineralization properties. [Vet World 2009; 2(6.000): 231-233]
Efficacy of Ruchamax N in treatment of Digestive Disorders in Cow
Rajiv Walia,K.Ravikanth and S. Maini
Veterinary World , 2011,
Abstract: Therapeutic efficacy of Ruchamax N (M/s Dabur Ayurvet Ltd. India) against digestive disorders in 20 cow of 1 to 3 years was evaluated. Of these, five animals were reported with the history of simple indigestion and rest fifteen with the history of disease induced anorexia & in few accompanied with low milk yield also. The animals were treated with Ruchamax N@ 15 g orally twice daily for 5 days. Clinical signs were recorded so as to assess the time required for complete recovery. On the basis of observations, it was concluded that Ruchamax N is a efficacious in treatment of simple indigestion and disease induced anorexia in cattle. [Vet. World 2011; 4(3.000): 126-127]
Expression of PCNA in oral gingival epithelium of aggressive and, chronic periodontitis—A pilot study  [PDF]
Penapy Jyostna, Manchala Sesha Reddy, Manyam Ravikanth, Mandalapu Narendra, Chini Dorasani Dwarakanath
Open Journal of Stomatology (OJST) , 2013, DOI: 10.4236/ojst.2013.31021
Periodontal disease leads to loss of tissue homeostasis of tooth supporting structures. The mechanism of fibrous tissue degradation by various enzymes in chronic periodontitis and aggressive periodontitis was well documented but there is paucity of information regarding epithelial response. The aim of the present study was to evaluation of epithelial cell proliferation in Chronic Periodontitis, Aggressive Periodontitis and normal patients using Proliferating Cell Nuclear Antigen (PCNA). The study consists of 30 individuals who attended the Periodontia clinic of Vishnu Dental College. They were divided equally into 3 groups: chronic periodontitis (10) aggressive periodontitis (10) and normal individuals requiring gingival excision for crown lengthening (10). Excised gingival tissues were routinely processed and subjected to Immuno-histochemical evaluation using Proliferating Cell Nuclear Antigen (PCNA). The mean proliferation index was almost equal in Aggressive periodontitis patients (36.55) and normal individuals (37.02) but it was high in Aggressive Periodontitis patients when compared to chronic periodontitis group (18.86). Observations of this study indicate the possibility of subclinical inflammation in clinically normal gingiva and difference in the types of microorganism associated with chronic periodontitis and aggressive periodontitis.
Treatment with Y-27632, a ROCK Inhibitor, Increases the Proinvasive Nature of SW620 Cells on 3D Collagen Type 1 Matrix
Ramana Vishnubhotla,Shruthi Bharadwaj,Shan Sun,Vitali Metlushko,Sarah C. Glover
International Journal of Cell Biology , 2012, DOI: 10.1155/2012/259142
Abstract: The concept of using tissue density as a mechanism to diagnose a tumor has been around for centuries. However, this concept has not been sufficiently explored in a laboratory setting. Therefore, in this paper, we observed the effects of cell density and extracellular matrix (ECM) density on colon cancer invasion and proliferation using SW620 cells. We also attempted to inhibit ROCK-I to determine its effect on cell invasion and proliferation using standard molecular biology techniques and advanced imaging. Increasing cell seeding density resulted in a 2-fold increase in cell invasion as well as cell proliferation independent of treatment with Y-27632. Increasing collagen I scaffold density resulted in a 2.5-fold increase in cell proliferation while treatment with Y-27632 attenuated this effect although 1.5 fold increase in cell invasion was observed in ROCK inhibited samples. Intriguingly, ROCK inhibition also resulted in a 3.5-fold increase in cell invasion within 3D collagen scaffolds for cells seeded at lower densities. We show in this paper that ROCK-I inhibition leads to increased invasion within 3D collagen I microenvironments. This data suggests that although ROCK inhibitors have been used clinically to treat several medical conditions, its effect largely depends on the surrounding microenvironment. 1. Introduction Colon cancer is the third most commonly diagnosed cancer and the third leading cause of cancer death in both men and women in the USA [1, 2]. Today, there is a wide array of methods used to diagnose cancer including biopsy, endoscopy, and diagnostic imaging. Imaging techniques utilize the fact that tumorigenic tissue has a higher tissue density than the surrounding normal extracellular matrix (ECM). Thus, areas of increased tissue density are considered a warning sign of a potential malignancy [3–5]. With this strong link between tissue density and cancer, there has not been sufficient in vitro data, particularly for colon cancer, to fully understand this phenomenon. Two variables that affect mechanics of a tissue are cell and ECM density. Altering cell density induces cellular differentiation, proliferation, and even apoptosis [6, 7]; thus, cell density is one of the relevant parameters in cancer research. Previous studies have suggested that higher cell density environments significantly increase cell metastasis, especially colon 26, [8] and the initial seeding density affects differentiation of stem cells more than the cytokines and growth factors [9]. Similarly, mechanical induction done by altering the surrounding ECM alone
Higher Molecular Weight Polyethylene Glycol Increases Cell Proliferation While Improving Barrier Function in an In Vitro Colon Cancer Model
Shruthi Bharadwaj,Ramana Vishnubhotla,Sun Shan,Chinmay Chauhan,Michael Cho,Sarah C. Glover
Journal of Biomedicine and Biotechnology , 2011, DOI: 10.1155/2011/587470
Abstract: Polyethylene glycol (PEG) has been previously shown to protect against enteric pathogens and prevent colon cancer invasion. To determine if PEG could indeed protect against previously observed pro-invasive effects of commensal E. coli and EPEC, Caco-2 cells grown in an in vitro model of colon cancer were infected with strains of human commensal E. coli or EPEC and treated with 10% PEG 3350, PEG 8000, and PEG 20,000, respectively. At 24 hours after infection, MMP-1 and MMP-13 activities, cell cluster thickness, depth of invasion, and proliferation were determined using standard molecular biology techniques and advanced imaging. We found that higher molecular weight PEG, especially PEG 8000 and 20,000, regardless of bacterial infection, increased proliferation and depth of invasion although a decrease in cellular density and MMP-1 activity was also noted. Maximum proliferation and depth of invasion of Caco-2 cells was observed in scaffolds treated with a combination of commensal E. coli strain, HS4 and PEG 8000. In conclusion, we found that PEG 8000 increased cell proliferation and led to the preservation of cell density in cells treated with commensal bacteria. This is important, because the preservation of a proliferative response in colon cancer results in a more chemo-responsive tumor.
Avijit Ghosh,Ray J. Butcher,Shaikh M. Mobin,M. Ravikanth
Acta Crystallographica Section E , 2010, DOI: 10.1107/s1600536810014285
Abstract: Molecules of the title macrocycle, C36H22N4O4, are located on an inversion center. The porphyrin ring shows a wave-like conformation with adjacent pyrrole rings tilted above the porphyrin plane and the interporphyrin distance is 3.584 (3) . The dihedral angles between the meso-furyl groups and the porphyrin plane are 38.87 (7) and 48.29 (7)°; these are much smaller than those observed for meso-tetraphenylporphyrin, indicating that the meso-furyl groups are more inclined towards the porphyrin plane. The decrease in the dihedral angle is due to the presence of intramolecular hydroden bonding between the meso-fury O atom and the β-pyrrole CH group. Intramolecular N—H...N hydrogen bonds are also present.
A new Polyherbal formulation to control bacterial enteritis in poultry: a case study in Salmonella enteritidis induced experimental model
K.K.Baishya,Shivi Maini and K.Ravikanth
Veterinary World , 2008,
Abstract: An experiemental study was conducted in day old 150 VenCobb chicks to evaluate efficacy of polyherbal formulation in induced bacterial enteritis with Salmonella enteritidis. Birds were randomly divided into three groups: negative control, infected and untreated control & prophylactically treated group with AV/ADC/16 (14th-28th days). Salmonella infection was induced on day 21st. A significant decrease in overall growth, productivity, feed conversion and mortality was evident in untreated infected group in addition to severity of clinical signs. However, prophylactic administration of herbal formulation reduced mortality and clinical symptoms were mild to negligible. No negative effect on growth & performance was observed in treated group III. [Veterinary World 2008; 1(11.000): 333-334]
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