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Search Results: 1 - 10 of 5495 matches for " Rameez Hassan "
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Molecular Detection and Sequencing for S1 Glycoprotein Gene of Bronchitis Virus of 2016 Epidemic from Sindh and Punjab  [PDF]
Ahmad Umer Sultan, Muhammad Danish Mehmood, Rameez Hassan, Huma Anwar, Sana Noreen, Faisal Amin, Sajjad Hussain
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.912044
Abstract: Infectious Bronchitis (IB) is highly contagious disease of commercial poultry causing substantial economic loses by producing poor quality meat in broilers and effecting production in breeder birds. The causative agent has been reported as most hazardous pathogen among other infectious agent even after being immunized with multi-variant strain vaccine. Currently, different strain such as H-120, 4/91 and D274 have been used extensively for immunoprophylaxis against velogenic strain across Pakistan with minimal protection reported. In current study PCR analysis was used to investigate the molecular nature of IB isolates from Punjab and Sind province of Pakistan in 2016 epidemics. Total of 100 tracheal samples were considered for virus inoculation in 10 days old chicken embryonated eggs. The IBV infected amniotic fluid was neutralized with monoclonal antisera of H-120, 4/91 and D274 strains. The IBV screened samples were subjected for RNA extraction and subsequent to PCR using type specific primer of each strain. The amplified product of 840 bp was sequenced through Sanger sequencing. On the basis of PCR results, four similar amplified products from both regions were obtained showing similarities in agarose gel electrophoresis, but they differ from each other on the basis of nucleotides sequence. Phylogenetic analysis revealed that nucleotide sequences of isolates from Karachi were similar to the IBV H-120, Mass-41 and Connecticut 46 reference strains. Whereas, isolates from the Punjab province are analogous to the Mans-2, Mans-3, 9/41(UK) but did not show significant similarity with other reference strain. Therefore, it is recommended that use of M-41 and H-120 in vaccine production could be effective measure against velogenic infectious agent in Sindh particularly in Karachi, whereas, it would be better to incorporate either of the variant GQ281656.1, AY279533.1 in vaccine because of their highest level of resemblance with genetically sequenced isolates from Lahore and its surroundings.
Cirrhotic cardiomyopathy among patients with liver cirrhosis  [PDF]
Tilahun Belay, Todd Gress, Rameez Sayyed
Open Journal of Gastroenterology (OJGas) , 2013, DOI: 10.4236/ojgas.2013.38060
Abstract:

Introduction: Cirrhotic cardiomyopathy (CCM) is a clinical syndrome in patients with liver cirrhosis characterized by an abnormal and blunted response in cardiac output and contractility to physiologic, pathologic, or pharmacologic stress but a normal to increased cardiac response at rest [1-4]. Information on the epidemiology and natural history of CCM is limited. Methods: All patients with a diagnosis of cirrhosis (N = 451) seen at gastroenterology clinic over the four years were evaluated. CCM was defined using echocardiogram (ECHO) and electrocardiogram (ECG) criteria [1]. Patients with structural or ischemic heart disease or incomplete information were excluded (N = 220). Results: Among the 231 patients with cirrhosis, 118 (51.1%) met criteria for CCM, and no patient had this problem documented in their medical record. Those with CCM were older (62.7 vs 57.8 years; p < 0.001) and more likely to be female (55.8 vs 40.2%; p = 0.02) compared to those without CCM. The likelihood of CCM increased with each quartile of age (OR 1.6 per quartile; 95% CI 1.2-2.0). Patients with alcoholic and unknown causes of cirrhosis are more likely to have CCM, (p < 0.001). CCM was more commonly associated with alcohol abuse in men than women (49.1 vs 21.3%; p = 0.002). Conclusion: CCM, a diagnosis of exclusion, defined by ECHO and ECG criteria is a common problem among cirrhotic patients attending a gastroenterology practice. Advancing age and female gender were associated with a higher prevalence of CCM, but the cause of cirrhosis was not possibly limited by smaller sample size within cause-specific categories. CCM was not recognized by our clinicians, and routine screening tests were not performed. Provider awareness of CCM is needed since implementation of angiotensin receptor blocker and beta-blocker therapy early in the course of cirrhosis may modify the changes in cardiac function [5,6].

 

Adherence to the European Society of Cardiology (ESC) guidelines for chronic heart failure - A national survey of the cardiologists in Pakistan
Sana Shoukat, Saqib A Gowani, Ather M Taqui, Rameez Ul Hassan, Zain A Bhutta, Anum I Malik, Sajjad A Sherjeel, Quratulanne Sheheryar, Sajid H Dhakam
BMC Cardiovascular Disorders , 2011, DOI: 10.1186/1471-2261-11-68
Abstract: A cross-sectional survey was conducted in person from March to July 2009 to all cardiologists practicing in 4 major cities in Pakistan (Karachi, Lahore, Quetta and Peshawar). A validated, semi-structured questionnaire assessing ESC 2005 Guidelines for HF was used to obtain information from cardiologists. It included questions about awareness and relevance of HF guidelines (See Additional File 1). Respondents' management choices were compared with those of an expert panel based on the guidelines for three fictitious patient cases. Cardiologists were also asked about major barriers to adherence to guidelines.A total of 372 cardiologists were approached; 305 consented to participate (overall response rate, 82.0%). The survey showed a very high awareness of CHF guidelines; 97.4% aware of any guideline. About 13.8% considered ESC guidelines as relevant or very relevant for guiding treatment decisions while 92.8% chose AHA guidelines in relevance. 87.2% of respondents perceived that they adhered to the HF guidelines. For the patient cases, the proportions of respondents who made recommendations that completely matched those of the guidelines were 7% (Scenario 1), 0% (Scenario 2) and 20% (Scenario 3). Respondents considered patient compliance (59%) and cost/health economics (50%) as major barriers to guideline implementation.We found important self reported departures from recommended HF management guidelines among cardiologists of Pakistan.The prevalence of heart failure (HF) continues to increase despite efforts at primary and secondary prevention[1]. Moreover, management of heart failure is complicated and requires extensive experience and knowledge of the current guidelines in effect. This can be tasking as they are updated frequently in accordance with new research trials and evolving recommendations.Despite comprehensive guidelines being available, studies show failure of optimal management in patients[2-6]. Also, significant variation exists among hospitals in the i
A Novel Review on Security and Routing Protocols in MANET  [PDF]
Muhammad Kashif Nazir, Rameez U. Rehman, Atif Nazir
Communications and Network (CN) , 2016, DOI: 10.4236/cn.2016.84020
Abstract:
The origin of Mobile ad hoc network (MANET) was started in 1970 as packet radio network (PRNET), later on different researches were made on it in different ages. MANET works under no fixed infrastructure in which every node works likes a router that stores and forwards packet to final destination. Due to its dynamic topology, MANET can be created anywhere, anytime. As there are limited resources in MANET so it faces many problems such as security, limited bandwidth, range and power constraints. Due to this, many new routing protocols are proposed. This article examines different techniques to manage congestion control, security issues, different layers attacks, routing protocols and challenges that are faced by MANET.
Development of a Framework for Strategic Outsourcing in Developing Countries
Maaz Ahmed Qureshi,Muhammad Salman,Rameez Khalid
International Journal of Materials, Mechanics and Manufacturing , 2013, DOI: 10.7763/ijmmm.2013.v1.20
Abstract: Outsourcing is a phenomenon that has changed the global business scenario. A practically applicable framework has been developed and tested in order to provide a tool for manufacturing industries for making decision regarding outsourcing. The main purpose of this paper is to target those countries where outsourcing manufacturing is still a new concept and provide them with an easy to follow framework. This framework will make the process of decision analysis easier and reliable for them. The proposed framework is a step by step model which by using core competency check, decision matrix, balance score card models and cost equations makes sure that the organization makes the right decision regarding whether a particular activity should be outsourced or not.
Callogenesis and Organogensis Studies in Some Accessions of Saccharum officnarum L.
Smiullah .,Farooq Ahmad Khan,Abdullah .,Rameez Iftikhar
Journal of Agricultural Science , 2013, DOI: 10.5539/jas.v5n4p171
Abstract: The aim of the present study was to investigate the response of three sugarcane accessions viz., S-2003-US-824, S-2003-US-230 and SPF-246 for callogenesis and organogenesis. The genotypes showed high value of callus score ranging 2.37 to 2.7. Genotype S-2003-US-824 was highest callus producer with an average of 2.7 callus score per test tube. Genotypes S2003-US-230 and SPF-246 were statistically similar with an average 2.37 and 2.49 respectively. Concentrations of 2, 4 D from 1 5 mg/I were considered to be the good for callus induction with best performance at 3 mg/l. Leaf and pith explants showed good response to callus production but the leaf explant performed better with average callus score of 2.83 per test tube which is statistically different from pith explant (2.21). Thus, it can be inferred that leaf explant is a good source of callus induction than pith explant.
Detection of Somaclonal Variation in Micropropagated Plants of Sugarcane and SCMV Screening through ELISA
Abdullah .,Smiullah .,Farooq Ahmad Khan,Rameez Iftikhar
Journal of Agricultural Science , 2013, DOI: 10.5539/jas.v5n4p199
Abstract: Callogenesis is a helping tool in tissue culture for creating variation. The study was carried out to investigate the response of different sugarcane varieties to callogenesis in order to analyze variation for sugarcane mosaic virus resistance. Three sugarcane accessions viz HSF-242, S2003-US-623, and S2003-US-633 were used in this study. The genotypes showed high value of callus score ranging from 2.59 to 3.25. Genotype HSF-242 was highest callus producer with an average score of 3.25. Three different media were used for organogenesis. S2003-US-633 proved to be the most responsive to organogenesis with an average of 43.33%. Material was exposed to shooting and rooting media and somaclones were shifted to green house. The somaclones produced were screened for sugarcane mosaic virus by ELISA (enzyme linked immunosorbent assay). Among 10 parent plants and 32 somaclones of HSF-242, only four somaclones showed susceptibility to SCMV disease while seven (7) parents showed positive reaction to the disease. Somaclones produced were also analyzed by microsattelite molecular markers to check the variation between parents and somaclones produced at molecular level. Molecular markers are useful for assessment of variation and phylogenetic relationships. Out of thirty (30) fragments amplified with fifteen (15) primers used among the sugarcane somaclones and parents, eleven (11) bands were polymorphic while rest of the nineteen (19) bands were monomorphic. Therefore, it may be concluded from the present results that SSRs can be used for identification of somaclonal variation and the relationship between parents and Somaclones.
Signal Processing Algorithms for Down-Stream Traffic in Next Generation 10?Gbit/s Fixed-Grid Passive Optical Networks
Rameez Asif,Rabeea Basir,Ramshah Ahmad
Advances in OptoElectronics , 2014, DOI: 10.1155/2014/296781
Abstract: We have analyzed the impact of digital and optical signal processing algorithms, that is, Volterra equalization (VE), digital backpropagation (BP), and optical phase conjugation with nonlinearity module (OPC-NM), in next generation 10?Gbit/s (also referred to as XG) DP-QPSK long haul WDM (fixed-grid) passive optical network (PON) without midspan repeaters over 120?km standard single mode fiber (SMF) link for downstream signals. Due to the compensation of optical Kerr effects, the sensitivity penalty is improved by 2?dB by implementing BP algorithm, 1.5?dB by VE algorithm, and 2.69?dB by OPC-NM. Moreover, with the implementation of NL equalization technique, we are able to get the transmission distance of 126.6?km SMF for the 1?:?1024 split ratio at 5?GHz channel spacing in the nonlinear region. 1. Introduction Due to the increasing demand of bandwidth and capacity requirements from enterprises and households, the data rates of broadband access network will be required over 10?Gbit/s for each customer. Several passive optical network (PON) architectures have been proposed, that is, G-PON, E-PON, TDM-PON, and so forth, in order to remove the capacity bottleneck. Recently, the 10?Gbit/s long haul wavelength-division multiplexed- (WDM-) PON system has been demonstrated with coherent detection; this configuration represents a significant improvement with a receiver sensitivity of ?45?dBm (25 photons/bit) [1]. With the implementation of advanced modulation formats, that is, QPSK, QAM, and so forth, and multiplexing techniques, that is, dual-polarization and so forth, the system performance is limited due to fiber linear and nonlinear effects [2]. These effects are very much dominant at higher signal launch powers and in WDM systems with narrow channel spacing [3]. Rosenkranz and von Hoyningen-Huene presented the results of nonlinearity compensation in access networks; however, the results are limited to the transmitters where optical field is derived from the modulation current with the directly modulated laser (DML) rate equations; thus modulation nonlinearity and chirp are included [4] and are detected by direct detection method. In this paper, we have numerically analysed the transmission characteristics and the nonlinear equalization techniques by employing BP, VE, and OPC-NM in 10?Gbit/s DP-QPSK long haul WDM-PON transmission with coherent receivers, for downstream signals. Furthermore; the impact of nonlinear equalization algorithm on the transmission distance and split ratio factor is investigated. 2. Nonlinear Equalization Methods In this section, we
Development of a Risk Model for Abdominal Wound Dehiscence  [PDF]
Mujahid Ahmad Mir, Farzana Manzoor, Balvinder Singh, Imtiyaz Ahmad Sofi, Abu Zaved Rameez, Sheikh Imran Farooq
Surgical Science (SS) , 2016, DOI: 10.4236/ss.2016.710063
Abstract: Objectives: To identify independent risk factors for abdominal wound dehiscence and develop a risk model to recognize high risk patients. Methods: The samples studied were patients who underwent midline laparotomy in the department of surgery, SMHS Hospital Srinagar from March 2009 to April 2015. For each case of abdominal wound dehiscence, three controls were selected from a group of patients who had undergone open abdominal surgery as close as possible in time. Preoperative, perioperative, and postoperative variables and in-hospital mortality were studied for all patients. Cases were compared with controls using the chi-square test or the Mann-Whitney U-test for categorical or continuous data, respectively. Subsequently, multivariate stepwise logistic regression with backwards elimination test used to identify main independent risk factors of abdominal wound dehiscence. The resulting regression coefficients for the major risk factors were used as weights for these variables to calculate a risk score for abdominal wound dehiscence. Results: 140 cases of abdominal wound dehiscence were reported and compared with 420 selected controls. All variables that were significant in univariate analyses were entered in a multivariate stepwise logistic regression to determine which variables were significant independent risk factors. Major independent risk factors were male gender, chronic pulmonary disease, corticosteroid use, smoking, obesity, anemia, jaundice, ascites, and sepsis, type of surgery, postoperative coughing, and wound infection. Based on these findings, a risk model was developed. Conclusions: The model can give an estimate of the risk of abdominal wound dehiscence for individual patients. High-risk patients may be planned preventive wound closing with reinforcements as mesh.
A New Design Technique of CMOS Current Feed Back Operational Amplifier (CFOA)  [PDF]
Hassan Jassim
Circuits and Systems (CS) , 2013, DOI: 10.4236/cs.2013.41003
Abstract:

A new design technique employing CMOS Current Feedback Operational Amplifier (CFOA) is presented. This design approach applies CFA OTA as input stage cascaded with class AB cross-coupled buffer stage. The performance parameters of CMOS CFOA such as bandwidth, slew rate, settling time are extensively improved compared with conventional CFOA. These parameters are very important in high frequency applications that use CMOS CFOA as an active building block such as A/D converters, and active filters. Also the DC input offset voltage and harmonic distortion (HD) are very low values compared with the conventional CMOS CFOA are obtained. P-Spice simulation results using 0.35 μm MI-ETEC CMOS process parameters shows considerable improvement over existing CMOS CFOA simulated model. Some of the performance parameters for example are DC gain of 67.2 dB, open-loop gain bandwidth product of 104 MHz, slew rate (SR+) of +91.3 V/μS, THD of -67 dB and DC input offset voltage of -0.2 mV.

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