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Search Results: 1 - 10 of 53670 matches for " Raimundo Ger?nimo da; "
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Modelos de neovasculariza??o corneana em coelhos: análise morfológica
Parente, Daniel Ramos;Silva, Maria Rosa Bet de Moraes;Silva Júnior, Raimundo Gernimo da;Marques, Mariangela de Alencar;
Arquivos Brasileiros de Oftalmologia , 2003, DOI: 10.1590/S0004-27492003000500021
Abstract: purpose: to compare three experimental models of limbal stem cell (sc) removal in rabbits in relation to morphologic aspects. methods: in the present study, 54 rabbits (108 eyes) were divided into 3 experimental groups - (g1), (g2) and (g3). each group consisted of 18 rabbits whose left eyes were submitted to 3 different experimental techniques - (t1), (t2) and (t3), respectively. a control group was formed with the 54 remaining eyes (re) of the g1, g2 and g3 rabbits. four morphological parameters were assessed: epithelium, inflammatory response, vascularization and fibroblastic response. results: the limbal epithelium was not completely removed by t1, was almost totally removed by t2, and thoroughly removed by t3. corneal surface was healed by conjunctival phenotype (conjunctivalization) in the three groups. goblet cells started to appear on days 14 and their density peaked on day 28. the inflammatory response was more intense in g3 than in g1 and g2. regression was faster in g1 and similar in g2 and g3 contributing to corneal opacity specially on days 14 and 28. it was mainly concentrated at the stromal anterior half, whereas the posterior half was spared. it peaked on day 28 and decreased from that moment on. in the beginning, it was characterized by a predominantly polymorphonuclear infiltrate, which, on day 56, changed to mononuclear. the new vessels started appearing on days 7. at first, they permeated the stromal upper half and progressed toward the surface as the stromal edema decreased. in the three groups, at the end of the experiment, vascularization was superficial in the stroma; but no thick sclerotic-fibrous tissue nor actual scars, distinct from the corneal stroma, were formed. conclusions: conjunctivalization and neovascularization occurred in all experimental models. t2 and t3 seemed to be adequate models for the removal of limbal sc and most studied parameters showed similar results. t1 was found to be an adequate model for the partial-thickness re
Immunohistochemical evaluation of p53 and Ki-67 proteins in colorectal adenomas
Sousa, Walysson Alves Tocantins de;Rodrigues, Lusmar Veras;Silva Jr., Raimundo Gernimo da;Vieira, Fernando Lopes;
Arquivos de Gastroenterologia , 2012, DOI: 10.1590/S0004-28032012000100007
Abstract: context: the appearance of adenomas and their progression to adenocarcinomas is the result of an accumulation of genetic changes in cells of the intestinal mucosa inherited or acquired during life. several proteins have been studied in relation to the development and progression of colorectal cancer, including tumor protein p53 (p53) and antigen identified by monoclonal antibody ki-67 (ki-67). objective: to evaluate the expression of p53 and ki-67 in colorectal adenomas and correlate the observed levels with clinical and pathologic findings. method: the sample consisted of 50 adenomatous polyps from patients undergoing colonoscopy. after performing polypectomy, polyps were preserved in a formalin solution with 10% (vol./vol.) phosphate buffer, submitted for routine preparation of sections and slides and stained with hematoxylin and eosin. for each adenoma we then performed immunohistochemistry to detect specific p53 and ki-67 proteins using a streptavidin-biotin-peroxidase enzyme immunoassay. results: p53 was detected in 18% of the adenomas. the average ki-67 protein index (i.ki-67) was 0.49. a statistically significant difference was observed in p53 (p = 0.0003) and ki-67 (p = 0.02) expression between adenomas with low- and high-grade dysplasia, particularly for p53. the expression of ki-67 was greater in rectal adenomas than in colic adenomas (p = 0.02). no relationship was found between the expression of the two proteins in the sample. conclusion: the p53 protein is expressed in a proportion of adenomas, while the ki-67 protein was expressed in all adenomas. the expression of p53 was higher in adenomas with high-grade dysplasia. the expression of ki-67 was higher in rectal adenomas and in adenomas with high-grade dysplasia.
Modelos experimentais de deficiência limbar em coelhos: análise clínica
Parente, Daniel Ramos;Silva, Maria Rosa Bet de Moraes;Silva Júnior, Raimundo Gernimo da;Marques, Mariangela de Alencar;
Arquivos Brasileiros de Oftalmologia , 2002, DOI: 10.1590/S0004-27492002000200002
Abstract: purpose: to compare the clinical aspects of three experimental models of limbal stem cell (sc) deficiency in rabbits. methods: in the present study, 54 rabbits (108 eyes) were divided into 3 experimental groups - (g1), (g2) and (g3). each group consisted of 18 rabbits. the left eyes were submitted to 3 different experimental techniques - (t1), (t2) and (t3), respectively. a control group was formed with the 54 remaining eyes (re). in all 3 techniques n-heptanol was used. in t1, the epithelium was removed by applying n-heptanol for 5 minutes. in t2, the n-heptanol application was followed by a 360o conjunctival peritomy at 4 mm beyond the limbus and scraping of the episcleral tissue. in t3, besides the procedure followed in t2, a lamellar dissection towards the limbus was performed, including 1.5 mm of the peripheral cornea and 2 mm of the scleral surface. six clinical parameters of the corneas were studied: neovascularization, opacity, surface irregularity, epithelial wound healing, epithelial erosion, presence of granuloma and other injuries. results: corneal neovascularization started earlier in g1 and g2. it occurred heterogeneously, ranging from mild to intense, in 100% of the cases. it remained stable from the 28th day up to the end of the experiment (56th day). it was more intense in the upper and lower corneal quadrants and less intense in the nasal and temporal quadrants. corneal opacity and irregularity of the corneal surface were less intense in g1 than in g2 and g3, which were similar. reepithelialization started after a 3-day latency period in all the animals submitted to the three experimental techniques and was complete on day 7 in g1 and day 14 in g2 and g3. the rate of occurrence of corneal epithelial erosion and corneal stromal granuloma were similar in the 3 groups. conclusions: t2 and t3 seemed to be suitable experimental models for total limbal stem cell deficiency and most of the parameters showed similar results. t1 was found to be an appropria
Modelos experimentais de deficiência limbar em coelhos: análise clínica
Parente Daniel Ramos,Silva Maria Rosa Bet de Moraes,Silva Júnior Raimundo Gernimo da,Marques Mariangela de Alencar
Arquivos Brasileiros de Oftalmologia , 2002,
Abstract: Objetivo: Comparar em coelhos três modelos experimentais de destrui o das células germinativas (CG) do limbo corneano quanto a aspectos clínicos. Métodos: Foram utilizados 54 coelhos, 108 olhos, subdivididos em 3 grupos experimentais: (G1), (G2) e (G3), cada um formado pelos OE de 18 coelhos, submetidos às técnicas experimentais (T1), (T2) e (T3), respectivamente, e um grupo controle, formado pelos 54 olhos contralaterais (OD). Nas três técnicas foi utilizado o n-heptanol. Na T1, o n-heptanol foi aplicado por 5 minutos, para remo o do epitélio límbico. Na T2, além da aplica o do n-heptanol, realizou-se peritomia e remo o da conjuntiva perilímbica até 4 mm do limbo, juntamente com a escarifica o do tecido episcleral. Na T3, além dos procedimentos da T2, foi realizada dissec o lamelar do limbo abrangendo aproximadamente 1,5 mm na periferia da córnea e 2 mm na superfície escleral. Em todas as córneas dos animais foram estudados seis parametros clínicos: neovasculariza o, perda da transparência, irregularidade da superfície, repara o epitelial, eros o ou defeito epitelial, granuloma e outras les es corneanas. Resultados: A neovasculariza o corneana iniciou-se mais precocemente com a T1 e T2; ocorreu em 100% dos casos com as três técnicas, de forma n o homogênea, variando de leve a intensa; permaneceu estável a partir do 28o dia até o final do experimento (56o dia), foi maior nos quadrantes superior e inferior e menor nos quadrantes nasal e temporal. A perda da transparência e a irregularidade da superfície corneana foram menores com a T1 que com a T2 e T3, que foram similares entre si. Houve, nas três técnicas experimentais, latência de três dias para o início da reepiteliza o, que se completou com a T1 no 7o dia e com a T2 e T3 no 14o dia. Eros o epitelial corneana e granuloma corneano foram encontradas de forma similar nas três técnicas experimentais. Conclus es: A T2 e T3 mostraram-se adequadas como possíveis modelos de ampla remo o das CG límbicas, levando a resultados similares nos diversos parametros estudados. A T1 se mostrou adequada como modelo de remo o parcial do epitélio límbico. Ocorreu conjuntivaliza o e neovasculariza o nas três técnicas experimentais.
Modelos de neovasculariza o corneana em coelhos: análise morfológica
Parente Daniel Ramos,Silva Maria Rosa Bet de Moraes,Silva Júnior Raimundo Gernimo da,Marques Mariangela de Alencar
Arquivos Brasileiros de Oftalmologia , 2003,
Abstract: OBJETIVOS: Comparar em coelhos três modelos experimentais de destrui o das células germinativas (CG) do limbo corneano quanto a aspectos morfológicos. MéTODOS: Foram utilizados 54 coelhos, 108 olhos, subdivididos em 3 grupos experimentais: (G1), (G2) e (G3), formados por 18 coelhos cada, que tiveram o OE submetido às técnicas experimentais (T1), (T2) e (T3), respectivamente, e um grupo controle, formado por 54 olhos contralaterais (OD) dos coelhos do G1, G2 e G3. Quatro parametros morfológicos foram estudados: epitélio, resposta inflamatória, vasculariza o e resposta fibroblástica. RESULTADOS: Com a T1 n o houve remo o da totalidade do epitélio límbico, com a T2 houve remo o da quase totalidade do epitélio límbico, com a T3 houve remo o da totalidade do epitélio límbico. A repara o da superfície corneana foi feita por epitélio de fenótipo conjuntival (conjuntivaliza o) com as três técnicas, havendo aparecimento de células caliciformes a partir do 14masculine dia, sendo a densidade maior no 28masculine dia. A resposta inflamatória com a T3 foi mais intensa que com a T1 e T2. A regress o foi mais rápida com a T1 e similar com a T2 e T3. Contribuiu para a turva o corneana principalmente no 14masculine e 28masculine dia, concentrando-se, principalmente, na metade estromal anterior, sendo poupada a metade posterior. Foi maior até o 28masculine dia, decrescendo a partir desse momento. Caracterizou-se, no início, por infiltrado com predomínio de polimorfonucleares e sofreu mudan a no 56masculine dia para infiltrado com predomínio de mononucleares. Os neovasos apareceram a partir do 7masculine dia, a princípio permearam o estroma do ter o médio para cima, progredindo para a superficializa o com a regress o do edema estromal. A vasculariza o com as três técnicas, ao final do experimento, foi superficial no estroma, porém n o houve a forma o de tecido fibro-escleroso denso ou cicatriz propriamente dita distinta do estroma corneano. CONCLUS ES: Ocorreu conjuntivaliza o e neovasculariza o nas três técnicas experimentais. As T2 e T3 mostraram-se adequadas como possíveis modelos de ampla remo o das CG límbicas, levando a resultados similares nos diversos parametros estudados. A T1 se mostrou adequada como modelo de remo o parcial do epitélio límbico.
Paracoccidioidomicose em hemisfério cerebral e tronco encefálico: relato de caso
Leal Filho Manoel Baldoino,Borges Guilherme,Silva Jr Raimundo Gernimo da,Aguiar Aline de Almeida Xavier
Arquivos de Neuro-Psiquiatria , 2006,
Abstract: Relata-se sobre um homem de 36 anos com passagem quatro anos antes pela selva amaz nica. Admitido após seis meses do aparecimento progressivo de tetraparesia, ataxia de marcha, disfagia, disartria, dispnéia e solu o. A ressonancia magnética revelou les o parietoccipital à direita e no bulbo, sendo esta última maior. Investiga es para tuberculose e síndrome da imunodeficiência adquirida tiveram resultados negativos. Foi submetido a microcirurgia da les o do bulbo. O estudo anatomopatológico revelou paracoccidioidomicose. Recebeu tratamento com anfotericina B até 2100 mg, e sulfametoxazol-trimetoprim por três meses, e fisioterapia. Voltou às atividades após seis meses do término do tratamento. Comenta-se sobre a participa o do sistema imunológico e das citocinas (interleucinas).
Preconditioning with L-alanyl-glutamine reduces hepatic ischemia-reperfusion injury in rats
Araújo Júnior, Raimundo José Cunha;Silva Júnior, Raimundo Gernimo da;Vasconcelos, Marcelo Pinho Pessoa de;Guimar?es, Sérgio Botelho;Vasconcelos, Paulo Roberto Leit?o de;Garcia, José Huygens Parente;
Acta Cirurgica Brasileira , 2011, DOI: 10.1590/S0102-86502011000700003
Abstract: purpose: to evaluate the effects of pre-conditioning with l-alanyl- glutamine (l-ala-gln) in rats subjected to total hepatic ischemia. methods: thirty wistar rats, average weight 300g, were randomly assigned to 3 groups (n=10): g-1 - saline, g-2- l-ala-gln, g-3-control (sham). g-1 and g-3 groups were treated with saline 2.0 ml or l-ala-gln (0.75mg/kg) intraperitoneally (ip) respectively, 2 hours before laparotomy. anesthetized rats were subjected to laparotomy and total hepatic ischemia (30 minutes) induced by by clamping of portal triad. control group underwent peritoneal puncture, two hours before the sham operation (laparotomy only). at the end of ischemia (g1 and g2), the liver was reperfused for 60 minutes. following reperfusion blood samples were collected for evaluation of alanine aminotransferase (alt) and lactate dehydrogenase (ldh) levels. liver (medium lobe) was removed for immunohistochemistry study with antibody for caspase-3. results: it was found a significant decrease (p<0.05) of alt levels (270.6 +40.8 vs 83.3 +5.5 - p <0.05), ldh (2079.0 +262.4 vs. 206.6 +16.2 - p <0.05) and caspase-3 expression (6.72 +1.35 vs. 2.19 +1.14, p <0.05) in rats subjected to i / r, comparing the group treated with l-ala -gln with g-2. also, the alt level was significantly lower (p<0.05) in g-1 and g-2 groups than in g-3 (control group). conclusion: l-ala-gln preconditioning in rats submitted to hepatic i/r significantly reduces alt, ldh and caspase-3 expression, suggesting hepatic protection.
Schwanoma de plexo braquial: relato de dois casos
Leal Filho, Manoel Baldoíno;Aguiar, Aline de Almeida Xavier;Almeida, Bruno Ribeiro de;Dantas, Karoline da Silva;Vieira, Marcelo Adriano da Cunha e Silva;Morais, Ricardo Keyson Paiva de;Silva Júnior, Raimundo Gernimo da;
Arquivos de Neuro-Psiquiatria , 2004, DOI: 10.1590/S0004-282X2004000100030
Abstract: schwannomas, neurinomas or neurilemmomas are benign peripheral nerve tumors. the literature report some cases associated with neurofibromatosis 2. we report two cases of cervical schwannoma originating from the brachial plexus unassociated with neurofibromatosis. a 31-year-old woman presented with a mass in the right supraclavicular region, irradiating pain and distal tingling to percussion (tinel's sign) for 6 months. and a 52-year-old woman presented with pain in the cervical region and right arm for one year. both the patients underwent to a microsurgery with total resection of the lesion. histology of the surgical specimen confirmed the diagnosis of schwannoma. postoperatively, the patients had a good recovery.
Paracoccidioidomicose em hemisfério cerebral e tronco encefálico: relato de caso
Leal Filho, Manoel Baldoino;Borges, Guilherme;Silva Jr, Raimundo Gernimo da;Aguiar, Aline de Almeida Xavier;Almeida, Bruno Ribeiro de;Vieira, Marcelo Adriano da Cunha e Silva;Pinheiro, Luciana Maria Ribeiro;
Arquivos de Neuro-Psiquiatria , 2006, DOI: 10.1590/S0004-282X2006000400033
Abstract: we report on a 36 years-old man that had been at the amazon forest four years before. six months before the admission he had developed a progressive quadriparesis, gait ataxia, dysphagia, dysarthria, difficulty in breathing and hiccup. the gadolinium-enhanced t1-weighted mri showed a lesion into the right parietoccipital area and another into the medulla, that was the largest. there was any evidence of tuberculosis or aids. the patient was submitted to microsurgical approach to the medulla. pathological examination revealed paracoccidioidomycosis. treatment with anphotericin b till 2100mg was administered followed by sulfamethoxazole-trimetoprim for three months plus physical therapy. the patient went back to his activities six months after the end of the treatment. comments are presented about the participation of the immunological system and of the cytokines (interleukines).
NMR Spectroscopy, Hammett Correlations and Biological Activity of Some Schiff Bases Derived from Piperonal
Echevarria, Aurea;Nascimento, Maria da Gra?a;Gernimo, Vanilde;Miller, Joseph;Giesbrecht, Astréa;
Journal of the Brazilian Chemical Society , 1999, DOI: 10.1590/S0103-50531999000100010
Abstract: a series of eleven schiff bases have been synthesized. they were obtained by condensation of piperonal (3,4-methylenedioxybenzaldehyde) with the corresponding aromatic primary amines. their 1h and 13c-nmr spectra have been obtained and the hammett correlations including chemical shifts and the subsitutent constants (sp, sr e si) were studied. linear and bilinear significant correlations were observed for iminic carbon (c-a) and c-1?, showing a more significant ressonance effect on chemical shifts. the chemical shifts for c-4? were highly affected by substituent effects, especially for halogens in the expected direction. their biological activity against microorganisms has also been measured and significant activity was showed against epidermophyton floccosum. the biological activity did not give a reasonable relationship with electronic effects.
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