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Search Results: 1 - 10 of 453 matches for " RS Beltagi "
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Tissue factor pathway inhibitor in paediatric patients with nephrotic syndrome
FA Farid, AA Mohammed, RS Beltagi, HM Afify
South African Journal of Child Health , 2011,
Abstract: Background. Tissue factor pathway inhibitor (TFPI) is an endogenous protease inhibitor that regulates the initiation of the extrinsic coagulation pathway by producing factor Xa-mediated feedback inhibition of the tissue factor/factor VIIa (TF/VIIA) catalytic complex. Objectives. To evaluate plasma TFPI levels in paediatric patients with nephrotic syndrome (NS) and its correlation with disease activity. Subjects and methods. Fifteen nephrotic patients in relapse (proteinuria >40 mg/m2/h, hypo-albuminaemia and oedema) before initiating steroid therapy (group I) and another 15 nephrotic patients in remission after withdrawal of steroid therapy (group II) were compared with 15 age- and sex-matched healthy children (group III). Besides clinical evaluation and routine laboratory investigations of NS, tissue factor pathway inhibitor levels in plasma were measured by enzyme-linked immunosorbent assay (ELISA). Results. The plasma TFPI level was higher in nephrotic patients during relapse (group I) and during remission (group II) (mean 102.53 (standard deviation (SD) 14.23) and 82.93 (SD 3.83) ng/ml, respectively) compared with that in the control group (62.40 (SD 7.53) ng/ml) (p<0.0001). In children with NS the plasma TFPI level was higher during relapse (group I) compared with the level in remission (group II) (p<0.0001). There was a negative correlation between the plasma TFPI level and total protein and serum albumin, and a positive correlation between the plasma TFPI level and the urinary protein/creatinine ratio (p<0.05). Conclusion. NS was associated with increased level of plasma TFPI in comparison with the control group, and the increase was more apparent in patients with active disease.
Phytotoxicity of Methyl tert-butyl Ether to Common Bean (Phaseolus vulgaris L.) Plants
Mohamed S. Beltagi
Pakistan Journal of Biological Sciences , 2007,
Abstract: The current investigation was conducted to report on the phytotoxicity of methyl tert-butyl ether (MTBE) to common bean (Phaseolus vulgaris L. cv. Nebraska) plants. The two-week-old potted plants were subjected to four weekly soil applications of aqueous MTBE concentrations (0, 1, 10, 25 and 50 ml L-1). The root growth, flower and pod development were more sensitive to MTBE treatments; while, stem growth and photosynthetic pigments were more persistent to the toxicity of MTBE. The total number of protein bands/lane in SDS-PAGE protein profile was reduced by MTBE treatments. Two proteins of molecular weight 53.83 and 30.96 kDa were newly synthesized at the highest concentrations (25 and 50 ml L-1) of MTBE; while the syntheses of other proteins were completely inhibited with varying sensitivity to MTBE concentrations. The toxicity of MTBE concentrations caused progressive collapsing of epidermal and cortical tissues of the plant roots. MTBE is quite toxic to crop plants in contaminated soils of agricultural systems.
In vitro Technology for Embryo Rescue and Long-term Stocking of Mangrove Seedlings
Mohamed S. Beltagi
Pakistan Journal of Biological Sciences , 2001,
Abstract: The deterioration of mangrove ecosystems worldwide calls for new techniques of propagation to improve tree potentials for seedling production. In the present technique, viable embryos were aseptically excised out of the mature propagules and then cultured on phytohormone-free MS medium. The produced seedlings exhibited relatively slow developmental growth rates which allowed an in vitro long-term stocking for these seedlings for more than 90 days with no need for reculturing
Phytotoxicity of Lead (Pb) to SDS-PAGE Protein Profile in Root Nodules of Faba Bean (Vicia faba L.) Plants
Mohamed S. Beltagi
Pakistan Journal of Biological Sciences , 2005,
Abstract: The present study was carried out to characterize and assess possible changes that might take place in the electrophoretic protein patterns involved in the N2-fixing root nodules of Vicia faba L.(faba bean) in response to lead (Pb) toxicity. The banding patterns of the SDS-PAGE protein profile in root nodules revealed both qualitative and quantitative changes. Lead treatments (PbO), either applied foliar or in soil, inhibited the number of polypeptides synthesized in the root nodules. The band intensity of the large subunit (240 kDa) of nitrogenase enzyme was greatly inhibited by all PbO treatments. Moreover, the synthesis of the small subunits (52 to 73 kDa) of nitrogenase was completely inhibited by both soil applications (500 and 2000 ppm) of PbO. The foliar applications (500 and 2000 ppm) of PbO greatly stimulated a 23 kDa protein (from 4.79 to 13.1 and 19.4%, respectively). This protein was also stimulated (only 2-folds) in the root nodules by PbO soil treatments. In conclusion, the reduced productivity of Vicia faba L. crop under Pb stress could be attributed to the impairment of N2-fixing enzymes.
Molecular responses of Bt transgenic corn (Zea mays L.) plans to salt (NaCl) stress
Mohamed Salah Beltagi
Australian Journal of Crop Science , 2008,
Abstract: Non-transformed and Bt transgenic crop lines of a hybrid (YieldGard 2) corn (Zea mays L.) plants in the 4th leaf stage were subjected to 0, 50, 100 and 150 mM NaCl. Significant (P = 0.05) reduction in growth of non-traformed plants was recorded under almost all levels (50, 100 and 150 mM) of salt (NaCl) stress; while, the growth of the Bt transgenic corn plants showed no significant changes under the same levels of salt stress. Chlorophyll a contents were reduced at 150 mM NaCl only; but did not change in the BT transgenic corn plants. Chlorophyll b was not responsive to NaCl treatments in both non-transformed and Bt transgenic corn plants. Chlorophyll stability index (CSI) were always higher in the Bt transgenic than in the non-transformed corn plants under all salinity levels. The analyses of SDS-PAGE revealed relative stability in the patterns of protein bands in the Bt transgenic corn plants under salinity stress. The sum of optical densities of protein bands was higher in the Bt transgenic corn plants. In response to salinity stress, a group of polypeptide (76.96, 59.38, 41.56, 33.5 and 31.26 KDa) were newly synthesized in both non-transformed and Bt transgenic plants. Salt-susceptible polypeptides of molecular weights 325.47, 32.64 and 24.17 KDa were found only in non-transformed corn plants and completely disappeared under all level of salt stress; while two polypeptides of molecular weights 38.59 and 30.61 KDa were totally inhibited in all salt-stressed corn plants. The synthesis of another four polypeptides (298.81, 99.82, 20.79 and 19.43 KDa) was solely specific to the Bt transgenic corn plants. Stability of chlorophyll pigments and molecular weights of salt stress responsive proteins are key genetic determinants of salt stress in Bt transgenic corn plants.
Square-Wave Adsorptive Cathodic Stripping Voltammeteric Determination of Manganese (II) Using a Carbon Paste Electrode Modified with Montmorillonite Clay  [PDF]
Amr M. Beltagi, Iqbal M. Ismail, Mohamed M. Ghoneim
American Journal of Analytical Chemistry (AJAC) , 2013, DOI: 10.4236/ajac.2013.44025
Abstract:

Manganese is an essential micronutrient for all organisms; however at high concentrations it has a toxic effect. Manganese toxicity is a serious constraint to crop cultivation since it is taken-up by plants and can easily be passed into the food chain again causing symptoms of Parkinson’s disease. A fully validated square-wave adsorptive cathodic stripping voltammetry method has been developed for determination of Mn (II) as a complex with 2-(5’-bromo-2’-pyridylazo) 5-diethylaminophenol in aqueous solutions using a carbon paste electrode (CPE) modified with montmorillonite-Na clay. The results showed that the modified CPE (90% (w/w) graphite powder and 10% (w/w) montmorillonite-Na clay) exhibited excellent electrochemical activity towards the investigated Mn (II) complex in acetate buffer of pH = 5.0. Factors affecting the performance of the modified carbon paste electrode and the sensitivity of the described square- wave stripping voltammetry method, including the electrode composition, concentration of ligand, pulse parameters and preconcentration conditions were examined. A detection limit (S/N = 3) of 0.015μg·L-1 (2.73 × 10-10 mol·L-1) Mn (II) was achieved when a preconcentration time of 240 s was applied. Insignificant interferences from various inorganic and organic species were estimated. The described square-wave adsorptive cathodic stripping voltammetry method coupled with the modified carbon paste electrode has been successfully applied to Mn (II) analysis in different water samples.

Current tissue engineering approaches to cartilage repair
RS Tuan
Arthritis Research & Therapy , 2004, DOI: 10.1186/ar1353
Abstract:
Farmers' awareness level of HIV/AIDS prevention in Ondo State, Nigeria: implication for food security
RS Olaleye
Journal of Agriculture and Social Research (JASR) , 2003,
Abstract: The study examined the level of HIV/AIDS awareness among rural farmers in Ondo State, Nigeria and its implication for food security. A multi-stage random sampling technique was used to select 200 respondents from 5 LGAs and 20 villages and descriptive statistics was used to analyze data collected. A total of 77.5% of the respondents were between the ages of 20 and 49 years, 52.5% were illiterate and only 35% were married. Out of 60% who were aware of HIV/AIDS, majority of them was aware of the use of condom (94.2%) to prevent infection. The first four sources of information about HIV/AIDS prevention indicated by the respondents were friends/relatives (98.3%) radio programmes (90.0%), church/mosque (80.8%) and personal interaction of farmers with extension agents (69.2%). It is recommended that governmental and non-governmental organizations as well as agricultural extension practitioners should intensify efforts in educating rural farmers about HIV/AIDS prevention. HIV/AIDS education should also be incorporated into extension programmes. (J Agric & Soc Res:2003 3(2): 1-11)
Should benefit–risk assessment have its own drug “label”?
Braithwaite RS
Drug, Healthcare and Patient Safety , 2011, DOI: http://dx.doi.org/10.2147/DHPS.S21927
Abstract: uld benefit–risk assessment have its own drug “label”? Perspectives (2561) Total Article Views Authors: Braithwaite RS Published Date August 2011 Volume 2011:3 Pages 37 - 41 DOI: http://dx.doi.org/10.2147/DHPS.S21927 R Scott Braithwaite Section of Value and Comparative Effectiveness, Division of General Internal Medicine, New York University School of Medicine, New York, NY, USA Abstract: Many consumers and clinicians incorrectly believe that the Food and Drug Administration (FDA) approval of a new therapeutic implies that its benefits have been proven to exceed its harms. While the FDA could require proof that benefits exceed harms prior to approval, it has been argued that this approach would be infeasible because of prohibitively large sample sizes. One possible alternative would be for the FDA to supplement its standard “label” denoting “safe and effective” with a secondary “label” denoting benefits have been demonstrated to exceed harms, which would be granted only after sufficient post-marketing data had accumulated to prove that its benefits exceeded its harms. This secondary label would not necessarily be linked to marketing restrictions or other commercial prohibitions but, rather, would be only information for consumers and clinicians. Strengths, weaknesses, and feasibility challenges of this approach are discussed.
Comparative studies of adipose triglyceride lipase genes and proteins: an ancient gene in vertebrate evolution
Holmes RS
Open Access Bioinformatics , 2012, DOI: http://dx.doi.org/10.2147/OAB.S27508
Abstract: mparative studies of adipose triglyceride lipase genes and proteins: an ancient gene in vertebrate evolution Review (2063) Total Article Views Authors: Holmes RS Published Date March 2012 Volume 2012:4 Pages 15 - 29 DOI: http://dx.doi.org/10.2147/OAB.S27508 Received: 21 October 2011 Accepted: 21 January 2012 Published: 08 March 2012 Roger S Holmes School of Biomolecular and Physical Sciences, Griffith University, Nathan, QLD, Australia Abstract: At least eight families of mammalian patatin-like phospholipase domain-containing proteins (EC 3.1.1.3) catalyze the hydrolysis of triglycerides, including adipose triglyceride lipase (ATGL), which functions in triglyceride lipase metabolism in the body, especially in adipose tissue. Bioinformatic methods were used to predict the amino acid sequences, secondary and tertiary structures, and gene locations for ATGL genes and encoded proteins using data from several vertebrate genome projects. ATGL genes usually contained nine coding exons for each of the vertebrate genomes examined, whereas the invertebrate sea squirt (Ciona intestinalis) ATGL gene contained a single exon. Vertebrate ATGL subunits contained 473–504 residues, shared >46% sequence identities, and exhibited sequence alignments and identities for key amino acid residues and predicted motifs: an N-terminal lipid binding region (residues 7–29 for human ATGL); a patatin "motif" (residues 10–179); a putative active site oxyanion "hole" (Cys15, Gly16, and Leu18); and catalytic dyad active site residues (Ser47 and Asp166). Predicted tertiary structures for the ATGL patatin "motif" were similar to those reported for potato patatin, suggesting that this structure is strongly conserved during animal and plant evolution. Human ATGL contained a CpG131 island within the gene promoter; miR-124/506 and miR-108 binding sites within the mRNA 3'-noncoding region; several transcription factor binding sites, including PPARA and PPARG, which are key regulators of genes encoding enzymes of lipid metabolism; and exhibited wide tissue expression at a higher than average level (2.2×). Phylogenetic analyses of vertebrate PNPLA-like gene families suggest that ATGL is an ancient gene in vertebrate evolution which has been derived from an ancestral ATGL gene (encoding adipose triglyceride lipase) and undergone successive gene duplication events, forming ancestral genes for vertebrate PNPLA1, ATGL, PNPLA3, PNPLA4, and PNPLA5 gene families.
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