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Search Results: 1 - 10 of 1380 matches for " RASHMI AGGARWAL "
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Rashmi Aggarwal
Indian Phytopathology , 2011,
Zonal Presidential Address (Delhi Zone)*-Progress and challenges towards reducing spot blotch disease of wheat - RASHMI AGGARWAL
Indian Phytopathology , 2012,
Morphological variability in different isolates of Chaetomium globosum
Indian Phytopathology , 2012,
Abstract: Chaetomium globosum, an ascomycetous fungus, belonging to the order Sordariales, family Chaetomiaceae has been identified as a potential antagonist of Bipolaris sorokiniana. The fungus produces numerous hairy perithecia in culture. The morphology of perithecia and ascospores has been studied by means of optical and scanning electron microscope. The perithecia were 180 x 153 to 316.8 x 284.8 11m, in isolates Cg2 and Cg4 respectively. The ascospores size of all the isolates were almost similar, smallest size being shown by isolate Cg9 (9.6 X 8.4 11m). Perithecia showed densely haired surfaces and the shape of perithecia varied from globose to subglobose. The ascospores released inside the perithecium were seen oozing out from osteole like cirrhus. Under SEM, the shape of perithecia could not be ascertained, as they were densely covered with stiff hairs having depositions. Ascospores under SEM were lemon shaped, smooth surfaced with apical papillae.
Identification of Bipolaris sorokiniana-responsive differential transcripts in wheat (Triticum aestivum L.)
Indian Phytopathology , 2011,
Abstract: Differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) is used to identify transcripts induced by Bipolaris sorokiniana infection in wheat (Triticum aestivum L.). Using this method, we have identified two mRNA transcripts that are up- or down-regulated following B. sorokiniana infection. With 12 primer combinations, a total of 93 differential display products were observed in resistant genotype, BH1146 compared to 89 differentially displayed products in susceptible genotype, Sonalika. These products demonstrated qualitative and quantitative differences in the expression. The differentially expressed transcripts were collectively named WTRB (Wheat Transcripts Responsive to Bipolaris). We have identified two WTRBs, which were significantly altered due to B. sorokiniana infection. Both WTRB-1 and WTRB-2 expressed for longer period in resistant line compared to the susceptible line and may be used as molecular markers for screening wheat lines for spot blotch resistance.
Molecular characterization of Chaetomium species using URP-PCR
Aggarwal, Rashmi;Sharma, Vandana;Kharbikar, Lalit L.;Renu,;
Genetics and Molecular Biology , 2008, DOI: 10.1590/S1415-47572008005000011
Abstract: keywords : chaetomium globosum; chaetomium reflexum; chaetomium perlucidum; genetic variability; urp-pcr.
Arora Rashmi,Kapoor Ramit,Gill N.S,Aggarwal Amit
International Research Journal of Pharmacy , 2011,
Abstract: Pharmacovigilance procedures are necessary for evaluation of medicines. Thus Pharmacovigilance aims at detection, assessment and prevention of adverse effects or of any possible drug related problems. Reports of suspected adverse drug reactions (ADRs) are the basis of pharmacovigilance surveillance of drugs. So there is need of method for summarising the important identified risks of a drug, important potential risks, and important missing information, including the potentially at-risk populations and situations where the product is likely to be used that have not been studied pre-approval. Need for a Pharmacovigilance Plan and sets out its principles of good practice for the design and conduct of observational studies.
Differential induction of defense related enzymes involved in lignin biosynthesis in wheat in response to spot blotch infection
Indian Phytopathology , 2012,
Abstract: Spot blotch of wheat caused by Bipolaris sorokiniana is prevalent in wheat growing areas of northern India. The role of lignification and the enzymes involved in the biosynthesis of lignin i.e. phenylalanine ammonia-lyase (PAL), cinnamyl alcohol dehydrogenase (CAD) and peroxidase (POX) in the wheat spot blotch disease resistance was studied using a resistant (Pusa-T 3336) and a susceptible genotype (Agra local). PAL activity began to increase in both the genotypes at 12 h after inoculation and reached to maximum at 2 days after inoculation (DAI). In resistant genotype Pusa-T 3336, 30 fold increase was found at 12 h after inoculation, whereas in susceptible genotype Agra local, the activity was marginal. CAD and POX activities were maximum at 2 DAI in resistant genotype, but in Agra local maximum CAD and POX activity was found at 4 DAI. POX activity was six times more in Pusa-T 3336 than Agra local. High lignification was observed in the resistant genotype.
Production of extracellular proteins and cellulases by different isolates of Chaetomium globosum Krunze ex. Fr.
Indian Phytopathology , 2011,
Abstract: Chaetomium globosum has been identified as a potential antagonist of several seed borne and soil borne pathogens. Nine isolates of C.globosum were examined for the presence of extra cellular proteins in culture filtrate by SDS-PAGE. All the isolates were found to be different with respect to the profile of extra cellular proteins. The dendrogram showed that isolates Cg2 and Cg3 resembled each other, while Cg5 and Cg6, and Cg7, Cg8 and Cg9 were similar and showed high degree of similarity forming a separate group. Isolates Cg1 and Cg4 did not resemble any other isolate. The six isolates i.e., Cg1, Cg2, Cg3, Cg4, Cg5 and Cg6, which showed more number of bands in protein profile were further screened for the production of cellulases in culture filterate. Filter Paper Activity (FPase) of these isolates revealed that maximum activity was observed after 9 days of incubation. The isolate Cg2 had the maximum activity (0.79 IU/ml) while Cg5 had least activity (0.48IU/ml). Similarly, the carboxy methyl cellulase (CMC-ase) activity was maximum in case of isolate Cg2 and productivity of cellobiase was also maximum in case of isolate Cg2 and least in Cg1 on ninth day of incubation.
Variability in sclerotial morphology of Rhizoctonia solani f.sp. sasakii incitant of banded leaf and sheath blight of maize as revealed through Scanning Electron Microscope
Indian Phytopathology , 2011,
Abstract: Ten isolates of Rhizoctonia solani f.sp. sasakii collected from major maize growing areas showed morphological variability. With respect to colour of the colony two groups were observed, one having isolates from Bhuvaneshwar, Jorhat, Pusa, Delhi, Ludhiana, and Dhaulakuan and other group having isolates from Murorissa, Almora, Pantnagar and Karnal. Based on the pattern of sclerotial production, the isolates were also divided into two groups; one bearing peripheral sclerotia and other group having isolates bearing scattered sclerotia. SclerotiaI aggregation was observed in all the isolates, although more in Karnal isolate. Pantnagar isolate produced significantly higher weight sclerotia (1.71mg) than all isolates. SEM of sclerotia showed pits in isolates from Bhubaneshwar, Jorhat, Delhi, Ludhiana and Karnal. Surface texture as seen by SEM varied from compact to loose.
Impact of physical and chemical factors on fungal contamination causing vegetable rots and their prevention under in vitro conditions
Indian Phytopathology , 2012,
Abstract: Various microorganisms contaminate vegetables during harvest, storage, transport and marketing. Fungal contamination dominates especially those producing mycotoxins thereby reducing their market value. These mycotoxins also enter the food chain affecting human and animals. In present investigation the effect of temperature, incubation period, pH, carbon & nitrogen sources and different chemicals had been studied on fusarial growth and zearalenone production in vitro conditions by potential strain of Fusarium equiseti (Cords.) Sacc isolated from pointed gourd. Storage period, temperature and pH had promoted toxin elaboration and mycelial growth largely. The rate of mycelial growth was maximum between 10 to 13 days (0.28 g/day), which drastically declined between 16 to 20 days (0.03 g/day). Zearalenone accumulation showed cumulative effect with increasing temperature and attained maximum value at 30° C, when 7.93 and 11.42 ppm zearalenone recorded in filtrate and mycelium respectively. The maximum rate of mycelial growth (0.5 g/0.1 pH) occurred between 7 to 7.4 pH, while maximum toxin (9.90 ppm) was produced at 7.8 pH. Polysaccharides, supported only 1.5 ppm toxin (raffinose) as compared to monosacchrides 5.16 ppm toxin (mannose) in culture filtrate. Nitrogen source NH4 NO3 and NaNO2 supported only 0.71 and 0.92 ppm toxin, while KNO3 induced 5.71 ppm in filtrate. Benalate and bavistin (carbendazim) completely inhibited toxin production at 200 ppm, while tetracycline showed 62.71% inhibition at 50 ppm concentration.
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