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Search Results: 1 - 10 of 97 matches for " Preantral follicles "
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Morphologic and quantitative evaluation of preantral follicles of buffalo (Bubalus bubalis) in different reproductive phases
F.C.A. Carvalho,E. Oba,L.S. Leal
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2007.s2.691
Abstract: The objectives of the present study were to estimate the population of the normal and degenerated preantral follicles of buffaloes in different reproductive phases and to classify the different types of degeneration of ovarian preantral follicles. For this, 18 ovaries were divided in three groups: prepubertal, non pregnat and pregnant adult females. The ovaries were collected from a slaughterhouse (Frigol – Brazil) and processed for classic histological examination. The follicular population was estimated according to Cahill et al. (1979) and calculated according to Gougeon & Chainy (1987). Young buffaloes, pregnant and non pregnat adults presented an average number of 15.5, 3.7 and 8.9 preantral follicles with normal morphology and type I (the degeneration was observed just in oocyte) and II (oocyte and granulosa cells were degenerated) degeneration, respectively. This experiment demonstrated that the proportions of primordial, primary and secondary follicles were affected by reproductive phase. A significant difference was observed considering the proportions of preantral follicles with normal morphology and Type I degeneration in all reproductive phases evaluated. The primary follicles presented more percentage of Type I degeneration that the others follicles and the primordial and primary follicles presented more percentage of Type II degeneration in three reproductive phases evaluated.
Isolamento e caracteriza??o ultraestrutural de folículos pré-antrais de vacas da ra?a Nelore (Bos taurus indicus)
Basso, Andréa Cristina;Esper, Cesar Roberto;
Brazilian Journal of Veterinary Research and Animal Science , 2002, DOI: 10.1590/S1413-95962002000600007
Abstract: preantral follicles from 41 nelore breed cows were described quantitative and ultrastructurally in the study. in the average, 35,539.20 preantral follicles were mechanically isolated (tissue copper) per animal. follicles were processed for transmission electron microscopy. primordial follicles presented an oocyte surrounded by a flattened granulosa cell layer, with some granulosa cells showing cuboidal shape. other development stages were classified as primary follicles, with a granulosa cell layer around the oocyte and secondary stages, with oocyte surrounded by two or more cuboidal granulosa cell layer. primordial follicles showed an evident oocyte, with the nucleus located at an eccentric cytoplasm position and a well-defined nucleolus, with condensed chromatin areas. organelles were centered at the peri-nuclear area, showing mostly round shaped mitochondria. follicles under development showed an increasing number of organelles and elongated mitochondria. adherence zones, coated pits and endocitose vesicles maintained the oocyte-granulosa cell interfaces. the zona pellucida (zp) appeared in primary follicles, showed small microvilos and many vesicles, which permeated into the zp. secondary follicles presented clusters of cortical granules associated to golgi complexes. in conclusion, the mechanical method provide a sufficient amount of preantral follicles from nelore breed cows and, due to ultrastructural similarity with follicles from other breed, it is possible to use same protocols of culture, since the oocyte meiotic maturation is kept intact.
Isolamento mecanico de folículos ovarianos pré-antrais em cabras
Rodrigues, Ana Paula Ribeiro;Amorim, Christiani Andrade;Lucci, Carolina Madeira;Figueiredo, José Ricardo de;Gon?alves, Paulo Bayard Dias;Bem, Assis Roberto de;
Ciência Rural , 1998, DOI: 10.1590/S0103-84781998000300021
Abstract: for mechanical isolation of caprine preantral follicles, ovaries from 52 undefinined breed type goats were collected at a local slaughterhouse or cirurgically extracted. the method used for preantral follicle isolation was a direct mechanical procedure (dmp). briefly, ovaries were cut into small fragments using a tissue chopper adjusted to 500mm. the ovarian fragments were suspended 80 times with a pasteur pipette and then, the suspension was filtered successively through 500 and 100mm nylon mesh filters. the dmp resulted in a large number of isolated preantral follicles. on average, 1,067 ± 160 (mean number ± sem) preantral follicles were isolated per ovary. to increase the number of preantral follicles recovered per ovary, the ovarian fragments remaining in the 500 and 100mm nylon mesh filters were transferred to the tissue chopper, and the procedure was repeated (complementary mechanical procedure - cmp). the use of cm p resulted in a mean increment of 5.2% of the total preantral follicles isolated per ovary. the mean number (mean ± sem) of follicles isolated per ovary using the mechanical procedure (dpm + cpm) was 1,126 + 163. irrespective of the procedure used, the number of preantral follicles isolated was characterized by extreme individual variation. the limits minimum and maximum of this variation were 53 and 7,482, respectively. the diameter of follicles isolated showed a range from l5.0mm to 92.7 mm. in conclusion, these results showed, for the first time, that preantral follicles can be successfully isolated from caprine ovary by applying a mechanical procedure.
Períodos de digest?o enzimática para o resgate de folículos pré-antrais em ovários de fetos bovinos
Carambula, Silvia Ferreira;Gon?alves, Paulo Bayard Dias;Costa, Luis Fabiano Santos da;Figueiredo, José Ricardo;Neves, Jairo Pereira;Mondadori, Rafael Gianella;
Ciência Rural , 1998, DOI: 10.1590/S0103-84781998000400020
Abstract: the objective of this experiment was to determine the efficiency and the effect of different enzymatic digestion periods on the number and developmental stages of preantral follicles isolated from bovine fetal ovaries. the presence of basal membrane, surrounding granulosa cells-oocyte complex, was observed, after digestion, through histologycal study. for this, ovaries from bovine fetues, were collected in slaughterhouses, between 150 and 270 days of pregnancy. in the laboratory, the ovaries were cut into several fragments with surgical scissors and dissociated with pasteur pipette, having either 1000 or 500mm diameter. after these steps, the fragments were submitted to an enzymatic digestion with collagenase in a concentration of 200iu/ml of modified tcm 199, for periods of 20, 30 or 40 minutes. the results showed that determined the total number and the quantity of the different developmental stages of isolated preantral follicles were similar in the three different periods of enzymatic digestion. the histological analyses demonstrated that the enzymatic digestion periods did not affect the morphological structure of the follicles.
DESENVOLVIMENTO DE FOLíCULOS PRé-ANTRAIS BOVINOS IN VITRO EM MONOCAMADA DE CéLULAS OVARIANAS
Costa, Luís Fabiano Santos da;Gon?alves, Paulo Bayard Dias;Figueiredo, José Ricardo;Carámbula, Silvia Ferreira;Neves, Jairo Pereira;Montagner, Marcelo Marcos;
Ciência Rural , 2001, DOI: 10.1590/S0103-84782001000200022
Abstract: the aim of the present work was to determine the influence of ovarian cells in the in vitro development of preantral follicles (pf). the viability of monolayer ovarian cells and the effect of the serum in the survive of in vitro pf was also investigated. ovarian cells and pf were isolated from ovaries of bovine fetus between 6 and 8 months of pregnancy, obtained in a slaughterhouse. monolayer of ovarian cells were cultured in a modified tcm-199 in the presence and absence of fsh and its viability after incubation was determined with trypan blue. pfs were divided in four different treatments, cultured in modified tcm-199, containing serum of castrated steer (scs), scs in monolayer of ovarian cells (moc), moc with fsh or a defined medium with polyvinyl alcohol (pva) as macromolecule. the cellular viability was not affected by the presence or absence of fsh. however, pfs had a significant development (p<0.05) when cultivated in the presence of snc, mco and fsh, which was not observed in the other treatments. with these results, it was possible to conclude that fsh does not influence the viability of the ovarian cells when cultured in a monolayer for 20 days. preantral follicles, measuring between 4000 and 8000mm2 but not with size greater than 8000mm2, grow on ovarian cells in vitro, independently of fsh.
Influência de diferentes concentra??es de etileno glicol nO número de células da granulosa e morfometria de folículos pré-antrais inclusos em tecido ovariano de Bos taurus indicus, Linnaeus, 1758
Luna, Hélder Silva e;Lijeron, Luciana Alves;Costa, Rafaela Nelson da;
Ciência e Agrotecnologia , 2010, DOI: 10.1590/S1413-70542010000600030
Abstract: the cryopreservation of the ovarian preantral follicles could help the conservation of several domestic and wild animal species. the objective of this study was to verify the effect of different concentrations of ethylene glycol on the morphometry and number of granulosa cells of the preantral ovarian follicles. a toxicity test was conducted with strips of ovarian cortex using ethylene glycol (10, 20 or 40%). tissue analysis was done using classical histology techniques. the primordial follicles expose at a concentration of 40% showed reduction of the follicular diameter and oocyte when compared to the control (non exposing), 10 and 20% groups (p<0.05) and reduction in the number of granulosa cells compared to the control group (p<0.05). regarding the primary follicles no alteration could be observed (p>0.05). these results suggest that primary follicles are more resistant to the effect of ethylene glycol when compared to primordial ones.
SET protein up-regulated testosterone production in the cultured preantral follicles
Xu Boqun,Gao Lingling,Cui Yugui,Gao Li
Reproductive Biology and Endocrinology , 2013, DOI: 10.1186/1477-7827-11-9
Abstract: Background We found previously that the expression of SET gene was up-regulated in polycystic ovaries. Evidences suggested that SET protein was essential for regulating both the promoter activity of CYP17A1 and the biological activity of P450c17. In this study, we explored whether SET regulated androgen production in preantral follicles. Methods The mouse preantral follicles were cultured in vitro. Testosterone secretion and expression of steroidogenic enzymes were observed in the preantral follicles treated in vitro by SET overexpression and knockdown. Results Testosterone levels in the media of the AdCMV-SET infected follicles significantly increased, and the CYP17A1 and HSD3B2 expression also significantly increased (P < 0.05). Testosterone levels in AdSiRNA-SET infected group decreased, and so did CYP17A1 and HSD3B2 expression (P < 0.05). Conclusions SET played a positive role in regulating ovarian androgen biosynthesis by enhancing the transcription of steroidogenic enzymes CYP17A1 and HSD3B2, which maybe contribute to the hyperandrogenism in PCOS.
Impact of pituitary FSH purification on in vitro early folliculogenesis in goats
Magalh?es,D.M.; Araújo,V.R.; Lima-Verde,I.B.; Matos,M.H.T.; Silva,R.C.; Lucci,C.M.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.;
Biocell , 2009,
Abstract: porcine pituitary follicle stimulating hormone (pfsh) is known to regulate the production of growth factors that have an essential role in early foliculogenesis. however, the effects of different preparations of pfsh on the survival and development of caprine follicles are not yet known. the aim of this study was to evaluate the effects of different pfsh (stimufol? and folltropin?) on the in vitro survival and growth of caprine preantral follicles. pieces of caprine ovarian tissues were cultured for either one or seven days in a supplemented minimum essential medium, alone or containing either stimufol? (50 ng/ml) or folltropin? (10, 50, 100 and 1000 ng/ml). fresh control ovarian tissues as well as cultured tissued were processed for histological and ultrastructural studies. the results showed that after seven days, only stimufol? maintained follicular morphology similar to control. moreover, follicular degeneration was higher in medium alone or with folltropin? at 50, 100 and 1000 ng/ml. however, at day seven, the percentage of growing follicles was higher in 100 ng/ml of folltropin? than stimufol?. in conclusion, fsh preparations affect differently the performance of in vitro culture of caprine preantral follicles. stimufol? was better to preserve follicular morphology while folltropin? was more efficient to promote follicular growth.
Impact of pituitary FSH purification on in vitro early folliculogenesis in goats
D.M. Magalh?es,V.R. Araújo,I.B. Lima-Verde,M.H.T. Matos
Biocell , 2009,
Abstract: Porcine pituitary follicle stimulating hormone (pFSH) is known to regulate the production of growth factors that have an essential role in early foliculogenesis. However, the effects of different preparations of pFSH on the survival and development of caprine follicles are not yet known. The aim of this study was to evaluate the effects of different pFSH (Stimufol and Folltropin ) on the in vitro survival and growth of caprine preantral follicles. Pieces of caprine ovarian tissues were cultured for either one or seven days in a supplemented Minimum Essential Medium, alone or containing either Stimufol (50 ng/mL) or Folltropin (10, 50, 100 and 1000 ng/mL). Fresh control ovarian tissues as well as cultured tissued were processed for histological and ultrastructural studies. The results showed that after seven days, only Stimufol maintained follicular morphology similar to control. Moreover, follicular degeneration was higher in medium alone or with Folltropin at 50, 100 and 1000 ng/mL. However, at day seven, the percentage of growing follicles was higher in 100 ng/mL of Folltropin than Stimufol . In conclusion, FSH preparations affect differently the performance of in vitro culture of caprine preantral follicles. Stimufol was better to preserve follicular morphology while Folltropin was more efficient to promote follicular growth.
Preservation of goat preantral follicles in saline or coconut water solution
Costa, Sonia Helena Furtado;Santos, Regiane Rodrigues dos;Ferreira, Marcos Ant?nio Leal;Machado, Vanessa Porto;Rodrigues, Ana Paula Ribeiro;Ohashi, Otávio Mitio;Figueiredo, José Ricardo de;
Brazilian Journal of Veterinary Research and Animal Science , 2002, DOI: 10.1590/S1413-95962002000600009
Abstract: the present study investigated the efficiency of saline solution and coconut water solution in the preservation of goat preantral follicles enclosed in ovarian tissue, at different temperatures and for different incubation periods. at the slaughterhouse, the ovarian pair was divided into 19 fragments; one ovarian fragment was immediately fixed for histology (control-time zero). the other 18 ovarian fragments were preserved in both solutions at 4oc, 20oc or 39oc for 4 h, 12 h or 24 h. the histological analysis showed that the storage of ovarian fragments in both solutions at 4oc for up to 24 h kept the percentage of normal preantral follicles similar to the control values. in contrast, preservation at 20°c or 39oc, in either solution, reduced significantly the percentage of normal preantral follicles compared to the control values, except in saline solution at 20oc for 4 h or in coconut water solution at 20oc for 4 h and 12 h. in conclusion, this study shows that both solutions can be used with the same efficiency to preserve goat preantral follicles at 4°c, irrespective of the incubation time. however, to preserve goat preantral follicles at higher temperatures, coconut water solution is recommended.
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