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In Silico Mining of EST-SSRs in Jatropha curcas L. towards Assessing Genetic Polymorphism and Marker Development for Selection of High Oil Yielding Clones  [PDF]
Neeraj Jain, Ganesh B. Patil, Poonam Bhargava, Rajani S. Nadgauda
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.511167

In recent years, Jatropha curcas L. has gained popularity as a potential biodiesel plant. The varying oil content, reported between accessions belonging to different agroclimatic zones, has necessitated the assessment of the existing genetic variability to generate reliable molecular markers for selection of high oil yielding variety. EST derived SSR markers are more useful than genomic markers as they represent the transcriptome, thus, directly linked to functional genes. The present report describes the in silico mining of the microsatellites (SSRs) using J. curcas ESTs from various tissues viz. embryo, root, leaf and seed available in the public domain of NCBI. A total of 13,513 ESTs were downloaded. From these ESTs, 7552 unigenes were obtained and 395 SSRs were generated from 377 SSR-ESTs. These EST-SSRs can be used as potential microsatellite markers for diversity analysis, MAS etc. Since the Jatropha genes carrying SSRs have been identified in this study, thus, EST-SSRs directly linked to genes will be useful for developing trait linked markers.

Cryptococcal granulomas in an immunocompromised HIV-negative patient
Taneja Juhi,Bhargava Aradhana,Loomba Poonam,Dogra Vinita
Indian Journal of Pathology and Microbiology , 2008,
Abstract: Disseminated cryptococcosis usually occurs in immunocompromised individuals with defective cell-mediated immunity, most commonly seen with HIV infection. We present a case of disseminated cryptococcosis in an HIV-negative male patient who presented with headache, fever, altered sensorium of short duration and multiple cutaneous lesions. An emergency CT scan of the head showed multiple intracranial and intraventricular granulomas. Routine laboratory investigations were within the normal range. A CSF examination revealed capsulated yeasts on India ink and a culture yielded cryptococcus neoformans. A cryptococcal antigen test by latex agglutination kit was positive. A biopsy revealed multiple capsulated yeasts cells in the cutaneous lesions, which were consistent with cryptococcus neoformans. The patient was successfully treated with Amphotericin B and Fluconazole with regression of cranial and cutaneous lesions.
Molecular characterization and the effect of salinity on cyanobacterial diversity in the rice fields of Eastern Uttar Pradesh, India
Ashish Srivastava, Poonam Bhargava, Arvind Kumar, Lal Rai, Brett A Neilan
Aquatic Biosystems , 2009, DOI: 10.1186/1746-1448-5-4
Abstract: Ten physicochemical parameters were analyzed for samples collected from twenty experimental sites. Electrical conductivity data were used to classify the soils and to investigate relationship between soil salinity and cyanobacterial diversity. The cyanobacterial communities were analyzed using semi-nested 16S rRNA gene PCR and denaturing gradient gel electrophoresis. Out of 51 DGGE bands selected for sequencing only 31 which showed difference in sequences were subjected to further analysis. BLAST analysis revealed highest similarity for twenty nine of the sequences with cyanobacteria, and the other two to plant plastids. Clusters obtained based on morphological and molecular attributes of cyanobacteria were correlated to soil salinity. Among six different clades, clades 1, 2, 4 and 6 contained cyanobacteria inhabiting normal or low saline (having EC < 4.0 ds m-1) to (high) saline soils (having EC > 4.0 ds m-1), however, clade 5 represented the cyanobacteria inhabiting only saline soils. Whilst, clade 3 contained cyanobacteria from normal soils. The presence of DGGE band corresponding to Aulosira strains were present in large number of soil indicating its wide distribution over a range of salinities, as were Nostoc, Anabaena, and Hapalosiphon although to a lesser extent in the sites studied.Low salinity favored the presence of heterocystous cyanobacteria, while very high salinity mainly supported the growth of non-heterocystous genera. High nitrogen content in the low salt soils is proposed to be a result of reduced ammonia volatilization compared to the high salt soils. Although many environmental factors could potentially determine the microbial community present in these multidimensional ecosystems, changes in the diversity of cyanobacteria in rice fields was correlated to salinity.The Indian agriculture is suffering with many man-made problems like canal irrigation, pesticide and chemical fertilization application. However, the former is responsible for salt accu
Some Wgh Inequalities for Univalent Harmonic Analytic Functions  [PDF]
Poonam Sharma
Applied Mathematics (AM) , 2010, DOI: 10.4236/am.2010.16061
Abstract: In this paper, some Wgh inequalities for univalent harmonic analytic functions defined by Wright's generalized hypergeometric (Wgh) functions to be in certain classes are observed and proved. Some consequent results are also discussed.
Spectral studies of transition metal complexes with 25, 26 dioxo1,6,12,17,23,24 hexaazacyclohexacosa 1,5,12,16 tetraene macrocyclic ligand (L)  [PDF]
Sulekh Chandra, Poonam Pipil
Open Journal of Inorganic Chemistry (OJIC) , 2013, DOI: 10.4236/ojic.2013.34013

Mn(II), Co(II), Ni(II) and Cu(II) complexes with this ligand (L) i.e. 25, 26 dioxo1,6,12,17,23,24 hexaazacyclohexacosa1,5,12,16 tetraene have been prepared. All the complexes were characterized by elemental analysis, molar conductance measurements, magnetic susceptibility measurements, mass, i.r. electronic, and e.p.r. spectral studies. The molar conductance measurements in DMF indicate that the Mn(II), Co(II), and Cu(II) are non-electrolyte whereas the complexes of Ni(II) are electrolyte. Thus, these complexes may by formulated as [M(L)X2] and [Ni(L)]X2 (where M = Mn(II), Co(II) and Cu(II) and X = C1- and \"\" ). All the complexes are of the high spin type expected Ni(II) complexes which are diamagnetic. On the basis of spectral studies, an octahedral geometry may be assigned of Mn(II) and Co(II). Square planar is for Ni(II) complexes, and tetragonal geometry for Cu(II) complexes.

Synthesis, Spectral Characterization and Biological Evaluation of Chromium(III) Complexes of Schiff Base  [PDF]
Sulekh Chandra, Poonam Pipil
Open Journal of Inorganic Chemistry (OJIC) , 2014, DOI: 10.4236/ojic.2014.42005
Abstract: Complexes of chromium(III) metal ion with schiff base i.e. 2,3,9,10 tetraphenyl-1,4,8,11-tetra- azacyclotetradeca-1,3,8,10 tetraene. (BPD) 2,4,10,12-tetramethyl-1,5,9,13-tetraazacyclohexadeca- 1,4,9,12 tetraene. (ADP) 2,3,9,10 tetramethyl 1,4,8,11 tatraazatetredeca-1,3,8,10 tetraene (DDP) have been synthesized and characterized by elemental analysis, molar conductance, magnetic susceptibility measurements, electronic and epr spectral studies. On the basis of elemental analysis the complexes were found to have composition CrLX3 (X = Cl-,\"\" , NCS-), Molar conductance, measured in DMF solution of these complexes indicates that the complexes are 1:1 electrolytes in nature, therefore the complexes may be formulated as [CrLX2]X. These complexes show magnetic moment in the range of 3.80 - 3.84 B.M. corresponding to three unpaired electrons. EPR and electronic spectral studies reveal that the complexes possess six-coordinated octahedral geometry. The ligand field parameters were calculated using various energy level diagrams. In vitro synthesized compounds and metal salts have also been tested against some species of plant pathogenic fungi and bacteria in order to assess their antimicrobial
Photon Emission from Quark Gluon Plasma at RHIC and LHC  [PDF]
Poonam Jain, Yogesh Kumar
Journal of Modern Physics (JMP) , 2014, DOI: 10.4236/jmp.2014.58080

We work on photon production through annihilation and Compton, and annihilation with scattering (AWS) processes through a quark-gluon plasma (QGP) in high energy nuclear collision incorporating the parametrization factors in strong coupling value and thermal dependent quark mass obtained through a coupling value. We find that photon yield increases with the increasing of the temperature and dominated by early times. We also compare the influence of coupling value on the spectrum of thermal photons. The production rate of photons is observed in the range of low and intermediate transverse momentum. The results are compared with other work.

Proline-leucine polymorphism of human glutathione peroxidase 1 in Thalassemia major  [PDF]
Vishal Bhargava, Manoj Gulati, Neelima Singh, Richa Bhargava
Advances in Bioscience and Biotechnology (ABB) , 2013, DOI: 10.4236/abb.2013.46091

Thalassemia is associated with low antioxidant enzyme deficiency especially glutathione peroxidase. GPX exists in 6 isomeric forms out of which GPX1 Single Nucleotide Polymorphism is found to be associated with Thalassemia major. In our study, the determination of the allelic frequency and phenotype of a common polymorphism in Se-dependent glutathione peroxidase 1 (GPX1) was observed in Thalassemic populations. A proline/leucine variant occurs at position 197 close to the C-terminus of the protein. The genotypes encoding Pro/Pro, Pro/Leu, and Leu/ Leu are distributed according to the Hardy-Weinberg relationship. The study has been carried out in 40 Thalassemic cases and 40 control subjects. No significant association between allele frequency and risk to get fatal was evident. Erythrocyte GPX activity was determined and no significant differences were obtained between the genotypes. It can be concluded that the Pro/Leu genetic variation does not appear to compromise the defense against oxidative stress in red blood cells or to be associated with significant pathology.

Synthesis, Characterization of Cellulose Grafted N-Oxide Reagent and Its Application in Oxidation of Alkyl/Aryl Halides  [PDF]
Inderjeet Kaur, Poonam K. Dhiman
International Journal of Organic Chemistry (IJOC) , 2011, DOI: 10.4236/ijoc.2011.11002
Abstract: Oxidation of aliphatic and aromatic halides by N-oxide functionalities supported on 4- vinyl pyridine, (4-VP), grafted cellulose is reported in the present manuscript. Synthesis of graft copolymer of cellulose and poly 4-vinyl pyridine, poly(4-VP), has been carried out using ceric ions as redox initiator. Post-grafting treatment of CellO-g-poly (4-VP) with 30% H2O2 in acetic acid gives Cellulose-g-poly (4-VP) N-oxide, the polymeric supported oxidizing reagent. The polymeric support, CellO-g-poly (4-VP) N-oxide, has been used for oxidation reactions of different alkyl / aryl halide such as 1-bromo-3-methyl butane, 2-bromo propane,1-bromo heptane and benzyl chloride. The polymeric reagent was characterized by IR and thermo-gravimetric analysis. The oxidized products were characterized by FTIR and H1NMR spectral methods. The reagent was reused for the oxidation of a fresh alkyl / aryl halides and it was observed that the polymeric reagent oxidizes the compounds successfully but with little lower product yield.
Group Action on Fuzzy Modules  [PDF]
Mohammad Yamin, Poonam Kumar Sharma
Applied Mathematics (AM) , 2016, DOI: 10.4236/am.2016.75038
Abstract: In this article, we introduce the notion of fuzzy G-module by defining the group action of G on a fuzzy set of a Z-module M. We establish the cases in which fuzzy submodules also become fuzzy G-submodules. Notions of a fuzzy prime submodule, fuzzy prime G-submodule, fuzzy semi prime submodule, fuzzy G-semi prime submodule, G-invariant fuzzy submodule and G-invariant fuzzy prime submodule of M are introduced and their properties are described. The homomorphic image and pre-image of fuzzy G-submodules, G-invariant fuzzy submodules and G-invariant fuzzy prime submodules of M are also established.
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