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Search Results: 1 - 10 of 1863 matches for " Pietro Iaffaldano "
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Low Serum Urate Levels Are Associated to Female Gender in Multiple Sclerosis Patients
Stefano Zoccolella, Carla Tortorella, Pietro Iaffaldano, Vita Direnzo, Mariangela D’Onghia, Elena Luciannatelli, Damiano Paolicelli, Paolo Livrea, Maria Trojano
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0040608
Abstract: Background Urate is a natural antioxidant and may prevent CNS tissue damage and the clinical manifestations of experimental autoimmune encephalitis. Results from clinical studies are conflicting and the contribution of urate to the pathogenesis of Multiple Sclerosis (MS) remains uncertain. Objective To evaluate serum urate levels in MS patients and their relationships with clinical, demographic and MRI variables. Methods Levels of non-fasting serum uric acid and creatinine were determined by an automated enzymatic assay and glomerular filtration rate was assessed in 245 MS patients, in 252 age/sex-matched neurological controls (NC) and in 59 Healthy controls (HC). Results Median serum urate levels did not differ between MS patients (3.8 mg/dL), HC (4.0 mg/dl) and NC (4.0 mg/dL). Serum urate levels were lower in females than in males in all groups (p = <0.0001). In female-MS, serum urate levels (3.2 mg/dL) were lower compared to those in female HC (3.8; p = 0.01) and NC (3.5 mg/dL; p = 0.02), whereas in male-MS they(4.8 mg/dL) did not differ from those in male HC (4.5 mg/dl) and NC (4.8 mg/dL). Urate concentrations trended to be lower in Clinically isolated syndromes suggestive of MS (3.7 mg/dL) and in relapsing MS (3.7 mg/dL), compared to patients with progressive MS (4.4 mg/dL; p = 0.06), and in patients with an annual relapse rate (ARR) >2 (3.3 mg/dL) than in those with an ARR ≤2: 3.9 mg/dL; p = 0.05). Significant lower serum urate levels were found in females than in males in all clinical MS subtypes (p<0.01), separately evaluated. Female sex (beta: ?0.53; p<0.00001) was the most significant determinant of serum urate concentrations in MS patients on multivariate regression analysis. Conclusions Our findings suggest that low urate levels could be of significance in predominantly inflammatory phases of MS even at the early stage and mainly in females.
Impact of Natalizumab on Cognitive Performances and Fatigue in Relapsing Multiple Sclerosis: A Prospective, Open-Label, Two Years Observational Study
Pietro Iaffaldano, Rosa Gemma Viterbo, Damiano Paolicelli, Guglielmo Lucchese, Emilio Portaccio, Benedetta Goretti, Vita Direnzo, Mariangela D'Onghia, Stefano Zoccolella, Maria Pia Amato, Maria Trojano
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0035843
Abstract: Background and Objectives Natalizumab reduces the relapse rate and magnetic resonance imaging activity in patients with Relapsing-Remitting Multiple Sclerosis (RRMS). So far the influence of natalizumab on cognitive functions and fatigue in MS remains uncertain. The aim of this prospective, open-label, observational study was to evaluate the possible effects of natalizumab on cognition and fatigue measures in RRMS patients treated for up to two years. Methods Cognitive performances were examined by the Rao's Brief Repeatable Battery (BRB), the Stroop test (ST) and the Cognitive Impairment Index (CII), every 12 months. Patients who failed in at least 3 tests of the BRB and the ST were classified as cognitively impaired (CI). Fatigue Severity Scale (FSS) was administered every 12 months to assess patient's self-reported fatigue. One hundred and 53 patients completed 1 and 2 year-natalizumab treatment, respectively. Results After 1 year of treatment the percentage of CI patients decreased from 29% (29/100) at baseline to 19% (19/100) (p = 0.031) and the mean baseline values of CII (13.52±6.85) and FSS (4.01±1.63) scores were significantly reduced (10.48±7.12, p<0.0001 and 3.61±1.56, p = 0.008). These significant effects were confirmed in the subgroup of patients treated up to two years. Conclusions These results demonstrate that a short-term NTZ treatment may significantly improve cognitive performances and fatigue in RRMS patients.
Speed of Adjustment and Infraday/Intraday Volatility in the Italian Stock and Futures Markets  [PDF]
Pietro Gottardo
Modern Economy (ME) , 2011, DOI: 10.4236/me.2011.25082
Abstract: We estimate the speed of adjustment of prices to value changes in the Italian stock and futures markets using variances in different return intervals. The paper presents evidence that an assumption of linearity for the relationship volatility-time is untenable when intraday and infraday data are used jointly. Prices adjust to new information within three days, but the process is complex with evidence of overshooting and divergent movements in the smaller return intervals. Firms behave differently according to their inclusion or exclusion from the MIB30 index. The speed of adjustment is strongly related to firm-specific characteristics and the log of capitalization explains some of the cross-sectional variability in the adjustment coefficients for most of the return intervals.
Coronary sinus reentrant tachycardia after atrial fibrillation ablation: From bad to worse  [PDF]
Pietro Turco
World Journal of Cardiovascular Diseases (WJCD) , 2014, DOI: 10.4236/wjcd.2014.42006
Abstract: Herein we present a case of atrial tachycardia as a sequel of AF ablations. A 42-year-old man was admitted to our department because of a very symptomatic tachycardia. The patient, because of paroxysmal AF and typical atrial flutter, had been already submitted (three times) to ablation procedures in both left (pulmonary vein insulation) and right atria (cavo-tricuspidal isthmus). During the electrophysiological study, a huge and very fast atrial tachycardia was induced: 230 ms cycle length, 1/1 atrio-ventricular conduction with the ventricular rate of 260 bpm, complete left bundle branch block, and clinically recognized by the patient. Four minutes later, a 2/1 AV conduction without branch block permitted mapping and ablation. A high-density mapping around isthmus and coronary sinus (CS) was performed. The analysis of the chronological activation clearly showed a circuit propagation around the CS ostium with a very slow conduction in the anterior zone enlightened by the tight color progression, and counterclockwise activation of the right atrium lateral wall. In anterior zone of CS ostium diastolic fragmented electrograms were detected. After preventing his position localization, radiofrequency delivering (35 W) was effective to interrupt the arrhythmia in 3 seconds. Energy delivering was continued to anchor the lesion to the inferior vena cava. Confirmation of successful ablation was determined by unsuccessful attempts at reinduction of the arrhythmia, in basal state and during infusion of isoproterenol.
A Theoretical Overview of Bioresponse to Magnetic Fields on the Earth’s Surface  [PDF]
Pietro Volpe
International Journal of Geosciences (IJG) , 2014, DOI: 10.4236/ijg.2014.510097
Abstract: This survey points to the mechanisms of bioresponse caused by magnetic fields (MFs), paying attention to their action not only on ions, molecules and macromolecules, but also on cells, tissues and organisms. The significance of findings concerning the MF-dependence of cell proliferation, necrosis or apoptosis was judged by comparing the results obtained in a solenoid, where an MF can be added to the geomagnetic field (GMF), with those obtained in a magnetically shielded room, where the MFs can be attenuated or null. This comparative criterion was particularly appropriate when the differences detectable between the data provided by experimental samples and the data provided by control samples were rather small, as observed in estimating the MF-influence on total DNA replication, RNA transcription and polypeptide translation. The MF-induced inhibition of apoptosis was considered as a risk potentially leading to accumulation of cancer cells. The analysis also surveyed the MF-dependence of the interactions between host animal cells and infecting bacteria. In relation to studies on the origin and adaptation of life on the Earth, theoretical insights paving the way to elucidating the MF-interactions with biostructures and biosystems of different orders of organization evaluated the possible involvement of the so-called “biological windows”. Analogously to what is known for ionizing radiations, the efficiency of the applied MFs appeared to depend on the complexity of their biological targets.
The Outrageous Discourse of Psychoanalysis for Present-Day Academic Institutions  [PDF]
Pietro Barbetta
Open Journal of Social Sciences (JSS) , 2015, DOI: 10.4236/jss.2015.33021
Abstract: In the following essay I’m going to take a radical position concerning the tendency to eliminate psychoanalysis from the European Academic field and the failure of psychoanalysts and relational therapists to defend psychoanalysis from such aggression. My question is why in Italy—just to make a local example, which I am involved in—different kinds of psychoanalytical traditions are not able to defend themselves from this attack while in France, for another example, all the different branches of relational therapies have been able to unite in making a common effort to take a position for psychoanalysis. One of the main problems, in my opinion, concerns the constitutive marginality of psychoanalysis in relation to Academic Institutions. In my way of writing, I will use psychoanalysis, with “P” in capital character, when referred to Academia, and psychoanalysis, with no capital character, when referred to clinical practice.
The Unexpected Existence of Coding and Non-Coding Fragments along the Eukaryotic Gene  [PDF]
Pietro Volpe
Advances in Biological Chemistry (ABC) , 2015, DOI: 10.4236/abc.2015.52009
Abstract: The pathways leading to synthesis and post-synthetic modification of DNA employed methionine as donor of atoms: the carbon that came from its –CH3 served for DNA replication and repair either in bacteria or humans; its entire –CH3 served instead for building N6-methyladenine and 5-methylcytosine on bacterial DNA and 5-methylcytosine alone on human DNA. In humans, although a slight extra-S asymmetric methylation appeared de novo yielding on parental DNA 5’-m5CpC-3’/ 3’-GpG-5’, 5’-m5CpT-3’/3’-GpA-5’ and 5’-m5CpA-3’/3’-GpT-5’ monomethylated dinucleotide pairs, a heavy symmetric methylation involved in S semiconservatively newly made DNA to guarantee genetic maintenance of –CH3 in 5’-m5CpG-3’/3’-Gpm5C-5’ dimethylated dinucleotide pairs. In this framework, an inverse correlation was found between bulk genomic DNA methylation occurring in S and bulk polyA-containing pre-mRNA transcription taking place in G1 and G2. Thus, probes of 1 × 106 Daltons (constructed using sheared by sonication newly made methylated DNA filaments) revealed a modular organization in genes: after the hypermethylated promoter, they exhibited an alternation of unmethylated coding and methylated uncoding sequences. This encouraged the search for a language that genes regulated by methylation should have in common. An initial deciphering of restriction minimaps with hypomethylatable exons vs. hypermethylatable promoters and introns was improved when the bisulfite technique allowed a direct sequencing of m5C. In lymphocytes, where the transglutaminase gene is inactive, its promoter exhibited two fully methylated CpG-rich domains at 5’ and one fully unmethylated CpG-rich domain at 3’, including the site +1 and a 5’-UTR. At variance, in HUVEC cells, where the transglutaminase gene is active, in the first CpG-rich domain of promoter few doublets lost their –CH3. Such an inverse correlation suggested new hypotheses especially in connection with repair-modification: UV radiation would cause demethylation in given loci of a promoter by chance, whilst even a partial demethylation in this promoter would be able to resume a previously silent pre-mRNA transcription.
Experimental and theoretical memory diffusion of water in sand
G. Iaffaldano, M. Caputo,S. Martino
Hydrology and Earth System Sciences (HESS) & Discussions (HESSD) , 2006,
Abstract: The basic equations used to study the fluid diffusion in porous media have been set by Fick and Darcy in the mid of the XIXth century but some data on the flow of fluids in rocks exhibit properties which may not be interpreted with the classical theory of propagation of pressure and fluids in porous media (Bell and Nur, 1978; Roeloffs, 1988). Concerning the fluids and the flow, some fluids carry solid particles which may obstruct some of the pores diminishing their size or even closing them, some others may chemically and physically react with the medium enlarging the pores; so permeability changes during time and the flow occurs as if the medium had a memory. In this paper we show with experimental data that the permeability of sand layers may decrease due to rearrangement of the grains and consequent compaction, as already shown qualitatively by Elias and Hajash (1992). We also provide a memory model for diffusion of fluids in porous media which fits well the flux rate observed in five laboratory experiments of diffusion of water in sand. Finally we show that the flux rate variations observed during the experiments are compatible with the compaction of sand, due to the amount of fluid which went through the grains locally, and therefore with the reduction of porosity. Final Revised Paper (PDF, 766 KB) Discussion Paper (HESSD) Citation: Iaffaldano, G., Caputo, M., and Martino, S.: Experimental and theoretical memory diffusion of water in sand, Hydrol. Earth Syst. Sci., 10, 93-100, doi:10.5194/hess-10-93-2006, 2006. Bibtex EndNote Reference Manager XML
Neuroglial cells in long-term primary cultures from the gilthead sea bream (Sparus aurata L.): new functional in vitro model from bony fish brain
Gerardo Centoducati,Valentina Zacchino,Marcella Narracci,Nicolaia Iaffaldano
Italian Journal of Animal Science , 2013, DOI: 10.4081/ijas.2013.e5
Abstract: Neuroglia has been historically considered the “glue” of the nervous system, as the ancient Greek name suggests, being simply referred as non-neuronal cells, with supporting functions for neurons in the CNS of mammalian and lower vertebrates. All around the world, approximately 283 cell lines were obtained from fish, yet none of these was from the brain of Sparus aurata, neither in cell lines nor as primary culture. Here we describe a novel in vitro reproducible neuroglial marine model for establishing primary neuroglial cell cultures, by dissociating the whole brain of seabream juveniles. We showed that proliferating neural stem cells produced alongside three generating lineages, such as neuronal precursor cells, astroglial precursor cells and oligodendroglia precursor cells, which developed respectively neurons, astrocytes and oligodendrocytes. The radial glia, finely described by morphological studies and immunochemical antigen expression, showed a peculiar spatial distribution, giving rise simultaneously both to astrocytes and neuronal precursors within a highly proliferative assemblate. Radial glia cells were assessed by glial fibrillary acidic protein (GFAP) and vimentin reactivity, astrocytes by GFAP, neurons by the neuron-specific markers for ubiquitin carboxy-terminal hydrolase 1 (UCHL1) and intermediate filament associated protein (NF), whereas myelinating oligodendrocytes were immunostained with anti-myelin basic protein (MBP) and anti-O4. Our findings suggest that seabream neuroglial cells gain in 3-4 weeks of culturing proliferation, neuroglial differentiation, and oligodendrocyte maturation with myelination, thus disclosing on the possibility that mixed neuroglial cultures can accelerate the maturation of oligodendrocytes and the regeneration of CNS injury in fish.
Comparison of different extenders on the quality characteristics of turkey semen during storage
N. Iaffaldano,M.P. Rosato,A. Manchisi,G. Centoducati
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2005.2s.513
Abstract: Semen of the domestic turkey cannot be stored longer than 6 h without a loss of fertilizing capability. The improvement of long-term liquid storage procedures of semen is important since the commercial production of turkey relies almost entirely on artificial insemination. Therefore, studies improving storage regimens would allow longer storage and consequently hen fertility (Iaffaldano and Meluzzi, 2003). Since the search for an optimal extender composition for semen storage is still in progress, the aim of this paper has been to study the effects of different extenders on the quality of turkey semen during the storage for 48 h at 5°C.
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