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Search Results: 1 - 10 of 101891 matches for " Pi-Hua Liu "
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Common sequence variants in CD36 gene and the levels of triglyceride and high-density lipoprotein cholesterol among ethnic Chinese in Taiwan
Chien Kuo-Liong,Hsu Hsiu-Ching,Liu Pi-Hua,Lin Hung-Ju
Lipids in Health and Disease , 2012, DOI: 10.1186/1476-511x-11-174
Abstract: Background Evidence of the genetic association between CD36 candidate gene and the risk of metabolic syndrome and its components has been inconsistent. This case–control study assessed the haplotype-tagged SNPs from CD36 on the risk of metabolic syndrome and components. Methods and results We recruited 1,000 cases and age, gender-matched controls were randomly selected from the participants with metabolic syndrome defined by International Diabetes Federation. Overall, the haplotype tagged SNPs of CD36 gene were not related to the risk of metabolic syndrome. For individuals with normal lipid levels, several SNPs were significantly associated with the triglycerides and HDL-cholesterol levels: Subjects with rs3211848 homozygote had a higher triglyceride level (99.16 ± 2.61 mg/dL), compared with non-carriers (89.27 ± 1.45 mg/dL, P = 0.001). In addition, compared with non-carriers, individuals with rs1054516 heterozygous and homozygous genotypes had a significantly lower HDL-cholesterol (46.6 ± 0.46 mg/dL for non-carrier, 44.6 ± 0.36 mg/dL for heterozygous, and 44.3 ± 0.56 mg/dL for homozygous, P = 0.0008). Conclusion The CD36 gene variants were significantly associated with triglycerides and HDL-cholesterol concentrations among ethnic Chinese in Taiwan.
Inference of Cross-Level Interaction between Genes and Contextual Factors in a Matched Case-Control Metabolic Syndrome Study: A Bayesian Approach
Shi-Heng Wang, Wei J. Chen, Lee-Ming Chuang, Po-Chang Hsiao, Pi-Hua Liu, Chuhsing K. Hsiao
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0056693
Abstract: Genes, environment, and the interaction between them are each known to play an important role in the risk for developing complex diseases such as metabolic syndrome. For environmental factors, most studies focused on the measurements observed at the individual level, and therefore can only consider the gene-environment interaction at the same individual scale. Indeed the group-level (called contextual) environmental variables, such as community factors and the degree of local area development, may modify the genetic effect as well. To examine such cross-level interaction between genes and contextual factors, a flexible statistical model quantifying the variability of the genetic effects across different categories of the contextual variable is in need. With a Bayesian generalized linear mixed-effects model with an unconditional likelihood, we investigate whether the individual genetic effect is modified by the group-level residential environment factor in a matched case-control metabolic syndrome study. Such cross-level interaction is evaluated by examining the heterogeneity in allelic effects under various contextual categories, based on posterior samples from Markov chain Monte Carlo methods. The Bayesian analysis indicates that the effect of rs1801282 on metabolic syndrome development is modified by the contextual environmental factor. That is, even among individuals with the same genetic component of PPARG_Pro12Ala, living in a residential area with low availability of exercise facilities may result in higher risk. The modification of the group-level environment factors on the individual genetic attributes can be essential, and this Bayesian model is able to provide a quantitative assessment for such cross-level interaction. The Bayesian inference based on the full likelihood is flexible with any phenotype, and easy to implement computationally. This model has a wide applicability and may help unravel the complexity in development of complex diseases.
Plasma fatty acids and the risk of metabolic syndrome in ethnic Chinese adults in Taiwan
Kuo-Liong Chien, Chia-Lun Chao, Chen-Hong Kuo, Hung-Ju Lin, Pi-Hua Liu, Pei-Rony Chen, Hsiu-Ching Hsu, Bai-Chin Lee, Yuan-Teh Lee, Ming-Fong Chen
Lipids in Health and Disease , 2011, DOI: 10.1186/1476-511x-10-33
Abstract: A nested case-control study based on 1000 cases of metabolic syndrome and 1:1 matched control subjects. For saturated fat, monounsaturated fat and transfat, the higher the concentration the higher the risk for metabolic syndrome: participants in the highest quintile had a 2.22-fold (95% confidence interval [CI], 1.66 to 2.97) higher risk of metabolic syndrome. In addition, the participants in higher EPA quintiles were less likely to have the risk of metabolic syndrome (adjusted risk, 0.46 [0.34 to 0.61] for the fifth quintile). Participants in the highest risk group (low EPA and high transfat) had a 2.36-fold higher risk of metabolic syndrome (95% CI, 1.38 to 4.03), compared with those in the lowest risk group (high EPA and low transfat). For prediction power, the area under ROC curves increased from 0.926 in the baseline model to 0.928 after adding fatty acids. The net reclassification improvement for metabolic syndrome risk was substantial for saturated fat (2.1%, P = 0.05).Plasma fatty acid components improved the prediction of the metabolic syndrome risk in Taiwan.Identifying dietary factors for the development of type 2 diabetes and metabolic syndrome is essential for primary prevention [1,2]. Dietary intake habits of fatty acids, including consumption of foods with high saturated fat and high transfat contents, are associated with insulin resistance and hyperlipidemia [3]. In addition, transfat has frequently been reported to be risk factors for cardiovascular diseases [4,5], and the evidence for monounsaturated fats was inconclusive[6]. In contrast, marine-derived fatty acids, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are inversely related to type 2 diabetes due to reduced inflammation and increased insulin sensitivity [7]. However, although there have been many studies about the association of specific fatty acids and the risk of cardiovascular diseases, there have only been a limited number of integrated comparison studies. Previous
Validation of Type 2 Diabetes Risk Variants Identified by Genome-Wide Association Studies in Han Chinese Population: A Replication Study and Meta-Analysis
Yi-Cheng Chang, Pi-Hua Liu, Yu-Hsiang Yu, Shan-Shan Kuo, Tien-Jyun Chang, Yi-Der Jiang, Jiun-Yi Nong, Juey-Jen Hwang, Lee-Ming Chuang
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0095045
Abstract: Background Several genome-wide association studies (GWAS) involving European populations have successfully identified risk genetic variants associated with type 2 diabetes mellitus (T2DM). However, the effects conferred by these variants in Han Chinese population have not yet been fully elucidated. Methods We analyzed the effects of 24 risk genetic variants with reported associations from European GWAS in 3,040 Han Chinese subjects in Taiwan (including 1,520 T2DM cases and 1,520 controls). The discriminative power of the prediction models with and without genotype scores was compared. We further meta-analyzed the association of these variants with T2DM by pooling all candidate-gene association studies conducted in Han Chinese. Results Five risk variants in IGF2BP2 (rs4402960, rs1470579), CDKAL1 (rs10946398), SLC30A8 (rs13266634), and HHEX (rs1111875) genes were nominally associated with T2DM in our samples. The odds ratio was 2.22 (95% confidence interval, 1.81-2.73, P<0.0001) for subjects with the highest genetic score quartile (score>34) as compared with subjects with the lowest quartile (score<29). The incoporation of genotype score into the predictive model increased the C-statistics from 0.627 to 0.657 (P<0.0001). These estimates are very close to those observed in European populations. Gene-environment interaction analysis showed a significant interaction between rs13266634 in SLC30A8 gene and age on T2DM risk (P<0.0001). Further meta-analysis pooling 20 studies in Han Chinese confirmed the association of 10 genetic variants in IGF2BP2, CDKAL1, JAZF1, SCL30A8, HHEX, TCF7L2, EXT2, and FTO genes with T2DM. The effect sizes conferred by these risk variants in Han Chinese were similar to those observed in Europeans but the allele frequencies differ substantially between two populations. Conclusion We confirmed the association of 10 variants identified by European GWAS with T2DM in Han Chinese population. The incorporation of genotype scores into the prediction model led to a small but significant improvement in T2DM prediction.
Genetic Variation in the NOC Gene Is Associated with Body Mass Index in Chinese Subjects
Yi-Cheng Chang, Yen-Feng Chiu, Pi-Hua Liu, Siow Wei Hee, Tien-Jyun Chang, Yi-Der Jiang, Wei-Jei Lee, Po-Chu Lee, Hui-Yi Kao, Juey-Jen Hwang, Lee-Ming Chuang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0069622
Abstract: Circadian clock genes are critical regulators of energy homeostasis and metabolism. However, whether variation in the circadian genes is associated with metabolic phenotypes in humans remains to be explored. In this study, we systemically genotyped 20 tag single nucleotide polymorphisms (SNPs) in 8 candidate genes involved in circadian clock, including CLOCK, BMAL1(ARNTL), PER1, PER2, CRY1, CRY2, CSNK1E,, and NOC(CCRN4L) in 1,510 non-diabetic Chinese subjects in Taipei and Yunlin populations in Taiwan. Their associations with metabolic phenotypes were analyzed. We found that genetic variation in the NOC gene, rs9684900 was associated with body mass index (BMI) (P = 0.0016, Bonferroni corrected P = 0.032). Another variant, rs135764 in the CSNK1E gene was associated with fasting glucose (P = 0.0023, Bonferroni corrected P = 0.046). These associations were consistent in both Taipei and Yunlin populations. Significant epistatic and joint effects between SNPs on BMI and related phenotypes were observed. Furthermore, NOC mRNA levels in human abdominal adipose tissue were significantly increased in obese subjects compared to non-obese controls. Conclusion Genetic variation in the NOC gene is associated with BMI in Chinese subjects.
Factors That Affect the Computational Prediction of Hot Spots in Protein-Protein Complexes  [PDF]
Jianping Lin, Pi Liu, Hua-Zheng Yang, Nagarajan Vaidehi
Computational Molecular Bioscience (CMB) , 2012, DOI: 10.4236/cmb.2012.21003
Abstract: Protein-protein complexes play an important role in the physiology and the pathology of cellular functions, and therefore are attractive therapeutic targets. A small subset of residues known as “hot spots”, accounts for most of the protein-protein binding free energy. Computational methods play a critical role in identifying the hotspots on the proteinprotein interface. In this paper, we use a computational alanine scanning method with all-atom force fields for predicting hotspots for 313 mutations in 16 protein complexes of known structures. We studied the effect of force fields, solvation models, and conformational sampling on the hotspot predictions. We compared the calculated change in the protein-protein interaction energies upon mutation of the residues in and near the protein-protein interface, to the experimental change in free energies. The AMBER force field (FF) predicted 86% of the hotspots among the three commonly used FF for proteins, namely, AMBER FF, Charmm27 FF, and OPLS-2005 FF. However, AMBER FF also showed a high rate of false positives, while the Charmm27 FF yielded 74% correct predictions of the hotspot residues with low false positives. Van der Waals and hydrogen bonding energy show the largest energy contribution with a high rate of prediction accuracy, while the desolvation energy was found to contribute little to improve the hot spot prediction. Using a conformational ensemble including limited backbone movement instead of one static structure leads to better predicttion of hotpsots.
Comment on the Invocation of Article XX for Violation of Para.11.3 in China—Raw Materials  [PDF]
Liu Hua
Beijing Law Review (BLR) , 2012, DOI: 10.4236/blr.2012.34021
Abstract: The failure to mention the relationship between China’s WTO-plus obligation in Paragraph 11.3 and the GATT 1994 does not mean the negotiators had meant to deny China the right to invoke Article XX of the GATT 1994. If that was the case, the intention would have been recorded in the Protocol and the Working Party Report. Contexts provided by the Working Party Report and provisions of the GATT 1994 and other provisions of the WTO Agreement prove that China cannot be denied the right to invoke Article XX. WTO-plus obligations are still integral parts of the WTO Agreement and the decision of the Panel and the AB in this case will lead to absurd results.
The diagnostic value of transvaginal three-dimensional volume ultrasound on uterus septus

LIU Li-hong,LIU Bing-bing,QIAO Hua,PI De-feng,

中华医学超声杂志(电子版) , 2010,
Abstract: Objective To investigate the value of the diagnosisfor simple and complicated uterus septus with transvaginal three-dimensional volume ultrasound. Methods The uterus septus images of transvaginal two-dimensional ultrasound and transvaginal three-dimensional volume ultrasound were retrospectively analysed in the 32 subjects who were already diagnosed by combined laparoscopic hysteroscopy or hysterectomy. Enrolled thirty-two patients included simple(without uterus neoplasm,intrauterine device and intrauterine...
Basic Elements Knowledge Acquisition Study in the Chinese Character Intelligent Formation System  [PDF]
Mingyou LIU, Chengsen DUAN, Youguo PI
Journal of Software Engineering and Applications (JSEA) , 2009, DOI: 10.4236/jsea.2009.25041
Abstract: In the Chinese character intelligent formation system without Chinese character library, it is possible that the same basic element in different Chinese characters is different in position, size and shape. The geometry transformation from basic elements to the components of Chinese characters can be realized by affine transformation, the transformation knowledge acquisition is the premise of Chinese character intelligent formation. A novel algorithm is proposed to ac-quire the affine transformation knowledge of basic elements automatically in this paper. The interested region of Chi-nese character image is determined by the structure of the Chinese character. Scale invariant and location invariant of basic element and Chinese character image are extracted with SIFT features, the matching points of the two images are determined according to the principle of Minimum Euclidean distance of eigenvectors. Using corner points as identifi-cation features, calculating the one-way Hausdorff distance between corner points as the similarity measurement from the affine image to the Chinese character sub-image, affine coefficients are determined by optimal similarity. 70244 Chinese characters in National Standards GB18030-2005 character set are taken as the experimental object, all the characters are performed and the experimental courses and results are presented in this paper.
Sufficient virus-neutralizing antibody in the central nerve system improves the survival of rabid rats
Liao Pi-Hung, Yang Hui-Hua, Chou Ping-Tse, Wang Ming-Hseng, Chu Po-Chun, Liu Hao-Li, Chen Li-Kuang
Journal of Biomedical Science , 2012, DOI: 10.1186/1423-0127-19-61
Abstract: The integrity of BBB permeability in rats was measured by quantitative ELISA for total IgG and albumin levels in the cerebrospinal fluid (CSF) and by exogenously applying Evans blue as a tracer. Western blotting of occludin and ZO-1, two tight junction proteins, was used to assess the molecular change of BBB structure.The breakdown of BBB with hypertonic arabinose, recombinant tumor necrosis factor-alpha (rTNF-γ), and focused ultrasound (FUS) were used to compare the extent of BBB disruption with rabies virus infection. Specific humoral immunity was analyzed by immunofluorescent assay and rapid fluorescent focus inhibition test. Virus-neutralizing monoclonal antibody (mAb) 8-10E was administered to rats with hypertonic breakdown of BBB as a passive immunotherapy to prevent the death from rabies.The BBB permeability was altered on day 7 post-infection. Increased BBB permeability induced by rabies virus infection was observed primarily in the cerebellum and spinal cord. Occludin was significantly decreased in both the cerebral cortex and cerebellum. The rabies virus-specific antibody was not strongly elicited even in the presence of clinical signs. Disruption of BBB had no direct association with the lethal outcome of rabies. Passive immunotherapy with virus-neutralizing mAb 8-10E with the hypertonic breakdown of BBB prolonged the survival of rabies virus-infected rats.We demonstrated that the BBB permeability was altered in a rat model with rabies virus inoculation. Delivery of neutralizing mAb to the infected site in brain combined with effective breakdown of BBB could be an aggressive but feasible therapeutic mode in rabies when the CNS infection has been established.
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