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Search Results: 1 - 9 of 9 matches for " Peti Thuwajit "
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Gene expression profiling of cholangiocarcinoma-derived fibroblast reveals alterations related to tumor progression and indicates periostin as a poor prognostic marker
Kusumawadee Utispan, Peti Thuwajit, Yoshimitsu Abiko, Komgrid Charngkaew, Anucha Paupairoj, Siri Chau-in, Chanitra Thuwajit
Molecular Cancer , 2010, DOI: 10.1186/1476-4598-9-13
Abstract: In this study, the gene expression profile of Cfs in comparison to Lfs was performed using oligonucleotide microarrays. The common- and unique-expressed genes in Cfs and the promising roles in cancer promotion and progression were determined. PN was markedly over-expressed in Cfs confirmed by real time RT-PCR and western blot analysis. Immunohistochemistry examination of a number of patients with intrahepatic CCA showed the expression of PN solely in stromal fibroblasts, but was expressed neither in cancer cells nor immune cells. Low to no expression of PN was observed in tissues of benign liver disease and hepatocellular carcinoma. CCA patients with high levels of PN had significantly shorter survival time than those with low levels (P = 0.026). Multivariate analysis revealed high levels of PN (P = 0.045) and presence of lymph node metastasis (P = 0.002) as independent poor prognostic factors. The in vitro study revealed that recombinant PN induced CCA cell proliferation and invasion. Interestingly, interference RNA against integrin α5 significantly reduced the cellular response to PN-stimulated proliferation and invasion.The gene expression profile of fibroblasts in CCA is apparently explored for the first time and has determined the genes involving in induction of this cancer progression. High PN can be used to distinguish CCA from other related liver diseases and is proposed as a prognostic factor of poor survival. Regulation of fibroblast-derived PN in CCA proliferation and invasion may be considered as an alternative therapeutic approach.Cholangiocarcinoma (CCA) originates from biliary epithelial cells and is a unique cancer in northeastern Thailand where the prevalence of a liver fluke, Opisthorchis viverrini infection is higher than elsewhere in the country. A recent study showed a strong positive correlation of CCA incidence and the prevalence of O. viverrini infection [1]. In other countries, CCA has been shown to correlate with Clonorchis sinesis [2,3], a
Analyze of the Possible Causes of Porosity Type Deffects in Aluminium High Pressure Diecast Parts
Ferencz Peti,Lucian Grama
Scientific Bulletin of the ''Petru Maior" University of T?rgu Mure? , 2011,
Abstract: Die casting is a metal casting process that is characterized by forcing molten metal under high pressure into a mold cavity. The mold cavity is created using two hardened tool steel dies which have been machined into shape and work similarly to an injection mold during the process. Most die castings are made from non-ferrous metals, specifically zinc, copper, aluminium, magnesium. Depending on the type of metal being cast, a hot- or cold-chamber machine is used.Die castings are characterized by a very good surface finish (by casting standards) and dimensional consistency.The most common deffect that appear in castings is the porosity type of deffect, which can be gas porosity, shrinkage porosity or leaker.
Solvolysis of the Tumor-Inhibiting Ru(III)-Complex trans-Tetrachlorobis(Indazole)Ruthenate(III)
Thomas Pieper,Wolfgang Peti,Bernhard K. Keppler
Metal-Based Drugs , 2000, DOI: 10.1155/mbd.2000.225
Interaction of Kinase-Interaction-Motif Protein Tyrosine Phosphatases with the Mitogen-Activated Protein Kinase ERK2
Dana M. Francis, Dorothy Koveal, Antoni Tortajada, Rebecca Page, Wolfgang Peti
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0091934
Abstract: The mitogen-activation protein kinase ERK2 is tightly regulated by multiple phosphatases, including those of the kinase interaction motif (KIM) PTP family (STEP, PTPSL and HePTP). Here, we use small angle X-ray scattering (SAXS) and isothermal titration calorimetry (ITC) to show that the ERK2:STEP complex is compact and that residues outside the canonical KIM motif of STEP contribute to ERK2 binding. Furthermore, we analyzed the interaction of PTPSL with ERK2 showing that residues outside of the canonical KIM motif also contribute to ERK2 binding. The integration of this work with previous studies provides a quantitative and structural map of how the members of a single family of regulators, the KIM-PTPs, differentially interact with their corresponding MAPKs, ERK2 and p38α.
The Molecular Mechanism of Substrate Engagement and Immunosuppressant Inhibition of Calcineurin
Simina Grigoriu,Rachel Bond,Pilar Cossio,Jennifer A. Chen,Nina Ly,Gerhard Hummer,Rebecca Page,Martha S. Cyert ,Wolfgang Peti
PLOS Biology , 2013, DOI: 10.1371/journal.pbio.1001492
Abstract: Ser/thr phosphatases dephosphorylate their targets with high specificity, yet the structural and sequence determinants of phosphosite recognition are poorly understood. Calcineurin (CN) is a conserved Ca2+/calmodulin-dependent ser/thr phosphatase and the target of immunosuppressants, FK506 and cyclosporin A (CSA). To investigate CN substrate recognition we used X-ray crystallography, biochemistry, modeling, and in vivo experiments to study A238L, a viral protein inhibitor of CN. We show that A238L competitively inhibits CN by occupying a critical substrate recognition site, while leaving the catalytic center fully accessible. Critically, the 1.7 ? structure of the A238L-CN complex reveals how CN recognizes residues in A238L that are analogous to a substrate motif, “LxVP.” The structure enabled modeling of a peptide substrate bound to CN, which predicts substrate interactions beyond the catalytic center. Finally, this study establishes that “LxVP” sequences and immunosuppressants bind to the identical site on CN. Thus, FK506, CSA, and A238L all prevent “LxVP”-mediated substrate recognition by CN, highlighting the importance of this interaction for substrate dephosphorylation. Collectively, this work presents the first integrated structural model for substrate selection and dephosphorylation by CN and lays the groundwork for structure-based development of new CN inhibitors.
Three Dimensional Structure of the MqsR:MqsA Complex: A Novel TA Pair Comprised of a Toxin Homologous to RelE and an Antitoxin with Unique Properties
Breann L. Brown equal contributor,Simina Grigoriu equal contributor,Younghoon Kim,Jennifer M. Arruda,Andrew Davenport,Thomas K. Wood,Wolfgang Peti,Rebecca Page
PLOS Pathogens , 2009, DOI: 10.1371/journal.ppat.1000706
Abstract: One mechanism by which bacteria survive environmental stress is through the formation of bacterial persisters, a sub-population of genetically identical quiescent cells that exhibit multidrug tolerance and are highly enriched in bacterial toxins. Recently, the Escherichia coli gene mqsR (b3022) was identified as the gene most highly upregulated in persisters. Here, we report multiple individual and complex three-dimensional structures of MqsR and its antitoxin MqsA (B3021), which reveal that MqsR:MqsA form a novel toxin:antitoxin (TA) pair. MqsR adopts an α/β fold that is homologous with the RelE/YoeB family of bacterial ribonuclease toxins. MqsA is an elongated dimer that neutralizes MqsR toxicity. As expected for a TA pair, MqsA binds its own promoter. Unexpectedly, it also binds the promoters of genes important for E. coli physiology (e.g., mcbR, spy). Unlike canonical antitoxins, MqsA is also structured throughout its entire sequence, binds zinc and coordinates DNA via its C- and not N-terminal domain. These studies reveal that TA systems, especially the antitoxins, are significantly more diverse than previously recognized and provide new insights into the role of toxins in maintaining the persister state.
Diuréticos del asa y ototoxicidad
Martínez-Rodríguez,R.; García Lorenzo,J.; Bellido Peti,J.; Palou Redorta,J.; Gómez Ruiz,J.J.; Villavicencio Mavrich,H.;
Actas Urológicas Espa?olas , 2007, DOI: 10.4321/S0210-48062007001000016
Abstract: loop diuretics are quite often used in medical practice. in spite of a wide security, there are several adverse events that should be known, in order to be early recognised as soon as they appear. ototoxicity associated to furosemide is infrequent and reversible. however, permanent deafness associated with furosemide administration has been previously reported.
Abordaje periauricular en las lesiones de la glándula parótida
Mijares Bri?ez,Alirio; Liuzzi,Francisco; Agudo Linares,Esteban; Brito Arreaza,Edgar; Garriga,Esteban; Peti,Henry; Suárez,José; Castro,Dilmery; Suárez,Carmen María;
Revista Venezolana de Oncología , 2011,
Abstract: objetives: to evaluate the possibility of resection of the lesions of the parotid gland by the incisions around the ear (group i) and assess immediate complications arising from this technique, the cosmetics results and the post operative pain, comparing them with the ahead ear - transcervical approach (group ii). methods: in 61 patients of both sex, from july 2006 to july 2009, were subjected to this prospective study, and randomized double blind, evaluating the two groups, categorized group i and ii, making comparisons on post operative by the analog levels of the pain, complications related to the facial nerve, possibility of resection regardless of the histological type, the affected lobe, and finally, the cosmetic satisfaction. results: the 59 patients that completed this study, no statistically significant differences we found in the post operative pain getting. there were no differences also on complications, but if in connection with the post operative scar, the 96 % patients (group i), spoke to us, their satisfaction with the cosmetic outcome compared with 65 % of group ii; that being statistically significant. conclusion: there was no differences as regards the presentation of the pain or the post operating complications in the around ear compared to the ahead ear - transcervical approach. the manifestation of cosmetic satisfaction in around ear were approach, we recommend it because it does not produce technical difficulties, without any distinction of sex, age, histological type, or location into the parotid gland.
A Novel Source of Cultured Podocytes
Stefano Da Sacco, Kevin V. Lemley, Sargis Sedrakyan, Ilenia Zanusso, Astgik Petrosyan, Janos Peti-Peterdi, James Burford, Roger E. De Filippo, Laura Perin
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0081812
Abstract: Amniotic fluid is in continuity with multiple developing organ systems, including the kidney. Committed, but still stem-like cells from these organs may thus appear in amniotic fluid. We report having established for the first time a stem-like cell population derived from human amniotic fluid and possessing characteristics of podocyte precursors. Using a method of triple positive selection we obtained a population of cells (hAKPC-P) that can be propagated in vitro for many passages without immortalization or genetic manipulation. Under specific culture conditions, these cells can be differentiated to mature podocytes. In this work we compared these cells with conditionally immortalized podocytes, the current gold standard for in vitro studies. After in vitro differentiation, both cell lines have similar expression of the major podocyte proteins, such as nephrin and type IV collagen, that are characteristic of mature functional podocytes. In addition, differentiated hAKPC-P respond to angiotensin II and the podocyte toxin, puromycin aminonucleoside, in a way typical of podocytes. In contrast to immortalized cells, hAKPC-P have a more nearly normal cell cycle regulation and a pronounced developmental pattern of specific protein expression, suggesting their suitability for studies of podocyte development for the first time in vitro. These novel progenitor cells appear to have several distinct advantages for studies of podocyte cell biology and potentially for translational therapies.
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