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Search Results: 1 - 10 of 44002 matches for " Peihua Wu "
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Acid Value Determination and Pre-Esterification of Crude Euphorbia lathyris L. Oil  [PDF]
Qiuyun Zhang, Jinfeng Wu, Peihua Ma, Jie Cai, Yutao Zhang
World Journal of Engineering and Technology (WJET) , 2015, DOI: 10.4236/wjet.2015.32007
Abstract: There were various problems in the determination of oil acid value of the national standard method, and this paper developed a low cost, simple and effective way to determine the acid value of oil and grease. Furthermore, the esterification of high acid Euphorbia lathyris L. oil (ELO) with methanol could be efficiently catalyzed by hydrochloric acid to produce biodiesel, and the influencing factors such as the amount of catalyst, reaction time, reaction temperature and molar ratio of oil to methanol were also studied. Under the optimized conditions with the oil to methanol molar ratio of 1:30 and a reaction temperature of 70, 95.8% oil conversion was obtained within 40 min in the presence of only 2.0 wt% of catalyst. Therefore, the low-cost non-edible Euphorbia lathyris L. oil as a raw material had good potential for the synthesis of biodiesel in industry.
The Conceptual Model of Influencing Factors and Influencing Mechanism on Team Decision-Making Quality Mediated by Information Sharing  [PDF]
Peihua Dai
iBusiness (IB) , 2013, DOI: 10.4236/ib.2013.54015
Abstract:

Searching for the influencing factors on team decision-making quality is the hot issue in academic circles and business circles. Based on summarizing the main influencing factors on team decision-making quality, this thesis divides the main influencing factors into team composition factors and team process factors and researches the internal relationship between them. Targeted at the field decision making, this thesis analyzes the influencing process of the main factors on decision-making quality, and constructs the conceptual model of “influencing factors-information sharing-decision-making quality”. This model not only combines the main influencing factors correctly, but also exposes the “black box” between the influencing factors and decision-making quality in field decision making, which can make up for no relative research for the field decision making in the previous studies.

Tensor-SIFT based Earth Mover's Distance for Contour Tracking
Peihua Li
Mathematics , 2010,
Abstract: Contour tracking in adverse environments is a challenging problem due to cluttered background, illumination variation, occlusion, and noise, among others. This paper presents a robust contour tracking method by contributing to some of the key issues involved, including (a) a region functional formulation and its optimization; (b) design of a robust and effective feature; and (c) development of an integrated tracking algorithm. First, we formulate a region functional based on robust Earth Mover's distance (EMD) with kernel density for distribution modeling, and propose a two-phase method for its optimization. In the first phase, letting the candidate contour be fixed, we express EMD as the transportation problem and solve it by the simplex algorithm. Next, using the theory of shape derivative, we make a perturbation analysis of the contour around the best solution to the transportation problem. This leads to a partial differential equation (PDE) that governs the contour evolution. Second, we design a novel and effective feature for tracking applications. We propose a dimensionality reduction method by tensor decomposition, achieving a low-dimensional description of SIFT features called Tensor-SIFT for characterizing local image region properties. Applicable to both color and gray-level images, Tensor-SIFT is very distinctive, insensitive to illumination changes, and noise. Finally, we develop an integrated algorithm that combines various techniques of the simplex algorithm, narrow-band level set and fast marching algorithms. Particularly, we introduce an inter-frame initialization method and a stopping criterion for the termination of PDE iteration. Experiments in challenging image sequences show that the proposed work has promising performance.
Increased intracellular calcium concentration causes electrical turbulence in guinea pig ventricular myocytes
XinRong Fan,JiHua Ma,Wei Wan,PeiHua Zhang,Chao Wang,Lin Wu
Science China Life Sciences , 2011, DOI: 10.1007/s11427-011-4146-1
Abstract: Dysregulation of intracellular Ca2+ homeostasis is associated with various pathological conditions and arrhythmogenesis of the heart. The objective of this study was to investigate the effects of an acute increase in intracellular Ca2+ concentration ([Ca2+]i) on the electrophysiology of ventricular myocytes by mimicking intracellular Ca2+ overload. The [Ca2+]i was clamped to either a controlled (65–100 nmol L 1) or increased (1 μmol L 1) level. The transmembrane action potentials and ionic currents were recorded using whole-cell patch clamp techniques. We found that the acute increase in [Ca2+]i shortened the action potential duration, reduced the action potential amplitude, maximum depolarization velocity and resting membrane potential, caused delayed after-depolarizations (DADs), and triggered activity—compared with these parameters in the control. The increased [Ca2+]i augmented late I Na in a time-dependent manner, reduced I CaL and I K1, and increased I Kr but not I Ks. The results of this study can be used to explain calcium overload-induced ventricular arrhythmias.
A SIMPLE METHOD OF SEPERATING,CULTIVATING AND OBSERVING MICROORGANISM
一种分离培养和观察微生物的简易方法

Wu Genfu,Chen Peihua,
吴根福
,陈佩华

微生物学通报 , 1994,
Abstract: This article introduced a simple method of seperating , cultivating and observingmicroorganism. It can be used for aerobe and aerotolerant anaerobe. The results showed thismethod has many adventages:(1) suits for doing research on individual growth of microorganismconsecutively;(2) can test death percentage of microorganism fast and simply;(3)suits forobtaining pure strain from mixed microorganisms effectively;(4) supplies a convenient methodfor the research of aerotolerant anaerobe.
Relationship between Interleukin-10 ?1082A/G Polymorphism and Risk of Ischemic Stroke: A Meta-Analysis
Jun Jin, Wuying Li, Lingmei Peng, Jian Chen, Rong Li, Peihua Wu, Sheng Tan
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0094631
Abstract: Objective To analyze the association between ?1082A/G polymorphism in interleukin-10 (IL-10) gene and ischemic stroke (IS) risk by meta-analysis. Methods We carried out a systematic electronic search in PubMed, BIOSIS Previews, Science Direct, Chinese National Knowledge Infrastructure, Chinese Biomedical Database, Weipu database and WANGFANG Database. Pooled odds ratios (ORs) with 95% confidence intervals (95%CIs) were calculated to assess the strength of the association. Results 7 studies were included. There was no significant association between IL-10 ?1082A/G polymorphism and IS risk under all genetic models in overall estimates (A vs. G: OR = 1.23,95%CI = 0.85–1.79;AA vs. GG: OR = 1.01,95%CI = 0.47–2.19; AG vs. GG: OR = 0.76, 95%CI = 0.38–1.55; AA+AG vs. GG: OR = 0.89,95%CI = 0.46–1.73; AA vs. AG+GG: OR = 1.39, 95%CI = 0.91–2.13). Similarly, no associations were found in subgroup analysis based on ethnicity and source of controls. However, removing the study deviating from Hardy–Weinberg equilibrium (HWE) produced statistically significant associations for overall estimates under recessive model(AA VS. AG+GG OR 1.58, 95% CI 1.04–2.42) and among Asians in all genetic models (A VS.G OR 1.64, 95% CI 1.07–2.53; AA vs. GG OR1.91, 95% CI 1.31–2.80; AG vs. GG OR1.44, 95% CI 1.09–1.91; AA+AG vs. GG OR 1.54, 95% CI 1.18–2.01;AA VS. AG+GG OR 1.79, 95% CI 1.07–3.00). Even after Bonferroni correction, the associations were observed still significantly in Asians under the two models (AA vs. GG OR1.91, 95% CI 1.31–2.80, P = 0.0008; AA+AG vs. GG OR 1.54, 95% CI 1.18–2.01, P = 0.001). Conclusion This meta-analysis indicates that IL10 ?1082 A/G polymorphism is associated with IS susceptibility in Asians and the ?1082 A allele may increase risk of IS in Asians. Considering the sample size is small and between-study heterogeneity is remarkable, more studies with subtle design are warranted in future.
Analysis of IL-17+ cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response
Heiner Appel, René Maier, Peihua Wu, Rebecca Scheer, Axel Hempfing, Ralph Kayser, Andreas Thiel, Andreas Radbruch, Christoph Loddenkemper, Joachim Sieper
Arthritis Research & Therapy , 2011, DOI: 10.1186/ar3370
Abstract: Immunohistochemical analysis of IL-17+ cells was performed in facet joints of 33 ankylosing spondylitis (AS) patients and compared with data from 20 osteoarthritis (OA) patients. The frequency of IL-17+CD4+ T cells in PB and SF of SpA patients (PB n = 30, SF n = 11), rheumatoid arthritis (RA) patients (PB n = 14, SF n = 7), OA patients (PB n = 10) and healthy controls (PB n = 12) was analysed after stimulation with Staphylococcus aureus Enterotoxin B and phorbol 12-myristate 13-acetate/ionomycin and quantified by flow cytometry.In AS facet joints, the frequency of IL-17-secreting cells was significantly higher than in samples obtained from OA patients (P < 0.001), with a slight predominance of IL-17+ cells among the mononuclear cells (61.5% ± 14.9%) compared to cells with polysegmental nuclei. Immunofluorescence microscopy revealed that the majority of IL-17+ cells were myeloperoxidase-positive (35.84 ± 13.06/high-power field (HPF) and CD15+ neutrophils (24.25 ± 10.36/HPF), while CD3+ T cells (0.51 ± 0.49/HPF) and AA-1+ mast cells (2.28 ± 1.96/HPF) were less often IL-17-positive. The frequency of IL-17+CD4+ T cells in the PB and SF of SpA patients did not differ significantly compared to RA patients, OA patients or healthy controls.Our data suggest an important role for IL-17 in the inflammatory processes in AS. However, the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response.Spondyloarthritis (SpA) comprises ankylosing spondylitis (AS), reactive arthritis, arthritis/spondylitis with inflammatory bowel disease and arthritis/spondylitis with psoriasis. Inflammatory back pain, a similar pattern of peripheral joint involvement with an asymmetrical arthritis predominantly of the lower limbs and the possible occurrence of sacroiliitis, spondylitis, enthesitis and uveitis are typical clinical features in this group of diseases [1]. SpA can be split into two categories, SpA with predominant axial involvement and SpA with pred
Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis
Heiner Appel, Wolfgang Kuon, Maren Kuhne, Peihua Wu, Stefanie Kuhlmann, Simon Kollnberger, Andreas Thiel, Paul Bowness, Joachim Sieper
Arthritis Research & Therapy , 2004, DOI: 10.1186/ar1221
Abstract: Chlamydia-triggered reactive arthritis (Ct-ReA) is strongly associated with HLA-B27 like other spondylarthropathies, and especially ankylosing spondylitis [1,2]. ReA occurs 1 to 4 weeks after urogenital infection with Chlamydia trachomatis or gastroenteral infection with enterobacteria such as Yersinia enterocolitica [3]. After acute onset, most patients have a self-limiting course, but up to 20% suffer from a disease duration of more than 1 year [4]. Of HLA-B27+-reactive arthritis patients, 20–40% move on to ankylosing spondylitis after 10–20 years, suggesting that the ReA-associated bacteria can cause ankylosing spondylitis [5] and that immune mechanisms triggering the disease are induced by T cell responses to microbial antigens. The main hypothesis advanced for the association between HLA-B27 and spondylarthropathies is the arthritogenic peptide theory. It states that some HLA-B27 subtype alleles, owing to their unique amino acid residues, bind a specific arthritogenic peptide that is recognized by CD8+ T cells [6-9]. Recently we and several other groups have reported on Chlamydia-specific CD8+ T cells capable of lysing target cells primed with Chlamydia antigens [10-12]. CD8+ T cell responses in spondylarthropathies other than Ct-ReA have also been described [13-15].Recently a new method for antigen-specific T cell recognition has been established by using multimerized MHC/peptide molecules [16]. These molecules are called tetramers because they contain four soluble and biotinylated MHC molecules linked to labelled streptavidin that specifically bind with high avidity to T cell receptors. In comparison with intracellular cytokine staining, the major advantage of tetramer technology is the identification of antigen-specific T cells independently of their cytokine secretion profile, the possibility of sorting unstimulated T cells and of having a tool for the antigen-specific detection of T cells in experiments in situ [17].In humans, MHC class I tetramers are wid
Resveratrol Attenuates the Na+-Dependent Intracellular Ca2+ Overload by Inhibiting H2O2-Induced Increase in Late Sodium Current in Ventricular Myocytes
Chunping Qian, Jihua Ma, Peihua Zhang, Antao Luo, Chao Wang, Zhiqiang Ren, Linghao Kong, Shuo Zhang, Xiaojing Wang, Ying Wu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0051358
Abstract: Background/Aims Resveratrol has been demonstrated to be protective in the cardiovascular system. The aim of this study was to assess the effects of resveratrol on hydrogen peroxide (H2O2)-induced increase in late sodium current (INa.L) which augmented the reverse Na+-Ca2+ exchanger current (INCX), and the diastolic intracellular Ca2+ concentration in ventricular myocytes. Methods INa.L, INCX, L-type Ca2+ current (ICa.L) and intracellular Ca2+ properties were determined using whole-cell patch-clamp techniques and dual-excitation fluorescence photomultiplier system (IonOptix), respectively, in rabbit ventricular myocytes. Results Resveratrol (10, 20, 40 and 80 μM) decreased INa.L in myocytes both in the absence and presence of H2O2 (300 μM) in a concentration dependent manner. Ranolazine (3–9 μM) and tetrodotoxin (TTX, 4 μM), INa.L inhibitors, decreased INa.L in cardiomyocytes in the presence of 300 μM H2O2. H2O2 (300 μM) increased the reverse INCX and this increase was significantly attenuated by either 20 μM resveratrol or 4 μM ranolazine or 4 μM TTX. In addition, 10 μM resveratrol and 2 μM TTX significantly depressed the increase by 150 μM H2O2 of the diastolic intracellular Ca2+ fura-2 fluorescence intensity (FFI), fura-fluorescence intensity change (△FFI), maximal velocity of intracellular Ca2+ transient rise and decay. As expected, 2 μM TTX had no effect on ICa.L. Conclusion Resveratrol protects the cardiomyocytes by inhibiting the H2O2-induced augmentation of INa.L.and may contribute to the reduction of ischemia-induced lethal arrhythmias.
Blind Deconvolution for Jump-Preserving Curve Estimation
Xingfang Huang,Peihua Qiu
Mathematical Problems in Engineering , 2010, DOI: 10.1155/2010/350849
Abstract: In many applications, observed signals are contaminated by both random noise and blur. This paper proposes a blind deconvolution procedure for estimating a regression function with possible jumps preserved, by removing both noise and blur when recovering the signals. Our procedure is based on three local linear kernel estimates of the regression function, constructed from observations in a left-side, a right-side, and a two-side neighborhood of a given point, respectively. The estimated function at the given point is then defined by one of the three estimates with the smallest weighted residual sum of squares. To better remove the noise and blur, this estimate can also be updated iteratively. Performance of this procedure is investigated by both simulation and real data examples, from which it can be seen that our procedure performs well in various cases.
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