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Search Results: 1 - 10 of 203445 matches for " Paulo Roberto Santana de Melo "
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N-acetyltransferase 2: Slow, intermediate or fast? A booming question of the molecular epidemiology in cancer research  [PDF]
Giuliano Di Pietro, Sandra Rocha Gadelha, Sandra Mara Bispo Sousa, Paulo Roberto Santana de Melo, Fabricio Rios Santos
Open Journal of Genetics (OJGen) , 2012, DOI: 10.4236/ojgen.2012.24028
Abstract: Throughout history, humanity has referred to reactions occurring with food, plants and, recently, medicines or drugs. The increase in pulmonary tuberculosis cases and the availability of treatment showed that genetic human differences can interfere in the capacity to metabolize drugs. There are remarkable genetic polymorphisms of N-acetyltransferase 2 (NAT2) activity that have been associated with different levels of susceptibility to developing many kinds of cancers. This review considers the field as an open window for the application of molecular epidemiology tools that led to the development of pharmacogenomics. We cover historical data and the most recent knowledge about NAT2 genetic polymorphisms and its distribution in different populations, which is an important concept being incorporated in epidemiological studies of cancer risk. We present up to date information about these studies, including meta-analysis based on the NAT2 distribution in different types of cancer. A critical broad at advances in NAT2 research, high-lighting recent studies related to NAT2 alleles in cancer susceptibility. Although there are multifactorial aspects involved in cancer risk, the variability in NAT2 allelic frequency can be related to carcinogenesis through alterations in the metabolic rate after exposure to carcinogens.
Investigation of polymorphisms in the genome of Dengue virus - DOI: 10.3395/reciis.v1i2.Sup.102en
Roberto Fabian Santos de Araújo,Nicolas Carels,Paulo Roberto Santana de Melo,Diego Frias
RECIIS : Electronic Journal of Communication, Information & Innovation in Health , 2007,
Abstract: In the present study, we investigated the nucleotide polymorphisms among the four genotypes of the Dengue virus - DENV-1, DENV-2, DENV-3 and DENV-4 - by qualitative and quantitative characterization. We studied the Nucleotide Substitution Density (NSD) along each genome identifying the regions with higher degree of mutation and/or conservation. Then, we calculated the Average Nucleotide Substitution Density (ANSD) for each serotype. We observed that the ANSD of DENV-2 is larger than for DENV-1 by 44.21%, DENV-3 by 85%, and DENV-4 by 163.31%. In contrast to DENV-2 and DENV-4, DENV-1 and DENV-3 showed a similar mutational behavior. The NS5 domain from DENV-2 that corresponds to the RNA-dependent RNA polymerase also has a higher mutation rate compared to that of the other DENVs. This suggests that the polymorphism and the virulence can be correlated in DENV-2, which could contribute to the understanding of the disease evolution.
Smooth muscle antibodies and cryoglobulinemia are associated with advanced liver fibrosis in Brazilian hepatitis C virus carriers
Andrade, Luis Jesuino de Oliveira;Melo, Paulo Roberto Santana de;Atta, Ajax Mercês;Atta, Maria Luiza Brito de Sousa;Jesus, Larissa Santana de;Sousa, Gabriel Menezes de;Silva, Carolina Alves Costa;Paraná, Raymundo;
Brazilian Journal of Infectious Diseases , 2011, DOI: 10.1590/S1413-86702011000100012
Abstract: cryoglobulinemia and non-organ-specific-autoantibody are biomarkers of autoimmunity of the chronic infection caused by hepatitis c virus (hcv). in this work, we report the association between the presence of smooth muscle antibodies (sma) and cryoglobulinemia and chronic liver disease in hcv carriers. sixty-five untreated hcv patients, 38 women and 27 men were included in this study. cryoglobulinemia was tested by cryoprecipitation, sma by indirect fluorescent antibody test, and liver fibrosis and hepatocellular inflammation activity was investigated by histology of liver biopsy using the metavir score. the prevalence of sma in the patients was 33.8% and cryoglobulinemia was demonstrated in 36.9% patients. cryoglobulinemia and sma seropositivity was associated with advanced fibrosis (p < 0.05). the presence of sma and cryoglobulinemia was not associated with hepatocellular inflammation activity, age, carrier gender or hcv genotype. we concluded that liver biopsy should be recommended for hcv carriers that are seropositive for sma or cryoglobulinemia.
The dynamics of dengue virus serotype 3 introduction and dispersion in the state of Bahia, Brazil
Melo, Paulo Roberto Santana de;Reis, Eliana Almeida Gomes;Ciuffo, Isolina Allen;Góes, M?nica;Blanton, Ronald Edward;Reis, Mitermayer Galv?o dos;
Memórias do Instituto Oswaldo Cruz , 2007, DOI: 10.1590/S0074-02762007000800003
Abstract: by 2002, dengue virus serotype 1 (denv-1) and denv-2 had circulated for more than a decade in brazil. in 2002, the introduction of denv-3 in the state of bahia produced a massive epidemic and the first cases of dengue hemorrhagic fever. based on the standardized frequency, timing and location of viral isolations by the state's central laboratory, denv-3 probably entered bahia through its capital, salvador, and then rapidly disseminated to other cities, following the main roads. a linear regression model that included traffic flow, distance from the capital and denv-1 circulation (r2 = 0.24, p = 0.001) supported this hypothesis. this pattern was not seen for serotypes already in circulation and was not seen for denv-3 in the following year. human population density was another important factor in the intensity of viral circulation. neither denv-1 nor denv-2 fit this model for 2001 or 2003. since the vector has limited flight range and vector densities fail to correlate with intensity of viral circulation, this distribution represents the movement of infected people and to some extent mosquitoes. this pattern may mimic person-to-person spread of a new infection.
Avalia??o de centrifugado osteogênico de medula óssea na consolida??o de fratura em coelhos
Vaz, Carlos Eduardo Sanches;Guarniero, Roberto;Santana, Paulo José de;
Acta Ortopédica Brasileira , 2010, DOI: 10.1590/S1413-78522010000600003
Abstract: objective: the purpose of this study was to evaluate the efficacy of a centrifuged osteogenic bone marrow aspirate to stimulate healing in rabbit fibular osteotomies. methods: ten white new zealand rabbits were used. a transverse medial diaphyseal fibular osteotomy was performed on the right fibula, where an absorbable collagen sponge embedded in osteogenic centrifuged bone marrow aspirate, obtained from the ipsilateral iliac bone, was inserted. the left fibula was used as the control group, where the collagen absorbable sponge was inserted without the osteogenic centrifuged aspirate. the rabbits were sacrificed four weeks after surgery to evaluate bone callus formation. analyses of results were performed with dexa bone densitometry to evaluate callus mineral mass, multislice computed tomography to evaluate callus volume and histomorphometry to evaluate the relative rate of tissue formation. results: the employment of centrifuged osteogenic bone-marrow aspirate resulted in a 40.3%, increase of callus bone mineral mass and increased relative quantity of bone tissue formation by 9.4%, without a significant increase in the relative quantities of cartilage, fibrous tissue, or in callus volume. conclusions: this study shows that the centrifuged osteogenic bone marrow aspirate was able to improve the healing of experimental fibular osteotomies in rabbits.
Fixa??o interna de fragmento osteocondral originado de osteocondrite dissecante do joelho
Vaz, Carlos Eduardo Sanches;Guarniero, Roberto;Santana, Paulo José de;Dal Molin, éden;Morandini, Paulo Henrique;
Acta Ortopédica Brasileira , 2008, DOI: 10.1590/S1413-78522008000400012
Abstract: the treatment of dissecting knee osteochondritis with crater-like lesion associated to intra-articular loose body can be provided by fixating the osteochondral fragment on its original site. however, there is some concern about the efficacy of this approach, since some reports have shown that the cartilage layer in osteochondral loose bodies that have been detached for a long time becomes deteriorated. we report a case where while the osteochondral loose body was detached for as long as five weeks, the fixation to the osteochondral defect was uneventful and resulted in a completely healed lesion. we concluded that, especially in cases in which the lesion involves a weight-bearing area of the knee with a detached osteochondral fragment, when the prognosis is usually worse if the fragment is discarded, potential complications of the fixation are offset by the benefits achieved by reducing the osteochondral fragment at the crater-like lesion, resulting in better joint congruence, and potentially avoiding an early joint degeneration process.
Avalia o da presen a de Staphylococcus aureus nos leitos do Centro de Terapia Intensiva do Hospital Escola da Faculdade de Medicina do Triangulo Mineiro, em rela o à posi o no colch o antes e após a limpeza
Mundim Guilhermo Justino,Dezena Roberto Alexandre,Oliveira Ana Carolina Santana de,Silva Paulo Roberto da
Revista da Sociedade Brasileira de Medicina Tropical , 2003,
Abstract: Através de meios de cultura, foi pesquisada a posi o de col nias de Staphylococcus aureus em colch es, visando avaliar a eficácia do procedimento de limpeza e desinfec o dos leitos do Hospital Escola da Faculdade de Medicina do Triangulo Mineiro (Uberaba). Foram analisadas amostras de 50 colch es no período de 22 de outubro de 2000 a 16 de janeiro de 2001. As amostras foram coletadas e semeadas, pela técnica de esgotamento, em dois meios de cultivo (ágar sangue e manitol) com posterior realiza o de provas de catalase e coagulase . Na análise estatística, foram utilizados os testes n o paramétricos Mann-Whitney, Kruswkal- Wallis e Wilcoxon Matched Pairs Test com nível de significancia p < 0,05. Foram utilizadas 600 placas de meio de cultivo. Houve crescimento em 94 (15,6%), sendo 82 (87,2%) antes e 12 (12,8%) após a limpeza e desinfec o. Em rela o à posi o no leito, as amostras semeadas no meio de cultivo com manitol mostraram que n o houve redu o significativa na posi o inferior do leito (p>0,05). Os resultados apontam e alertam para falhas no procedimento de limpeza e desinfec o dos leitos hospitalares por nós estudados.
ESTABELECIMENTO DE UM MODELO DE TUMOR EXPERIMENTAL PELA INOCULA O DO TUMOR DE WALKER EM EST MAGO DE RATO
Oliveira Paulo Ferdinando de Melo,Henriques Iuri Aderaldo,Rodrigues Filho Filadelfo,Almeida Paulo Roberto Carvalho de
Acta Cirurgica Brasileira , 1998,
Abstract: A incidência de cancer gástrico espontaneo em ratos é extremamente rara. Por outro lado, embora o cancer experimental de est mago possa ser induzido por carcinógenos químicos, através da ressec o da regi o fúndica do est mago, pela vagotomia ou pelo refluxo, esses métodos apresentam um percentual de desenvolvimento tumoral baixo e errático. Além disso, geralmente decorre um longo período de tempo até o pleno crescimento do tumor. O presente trabalho procurou estabelecer um modelo de tumor experimental de crescimento rápido, uniforme e com elevado índice de pega, que permitisse a avalia o de novas terapêuticas para o tratamento do cancer gástrico. Para tanto, utilizou-se uma suspens o de células do carcinossarcoma 256 de Walker que foi inoculada no est mago através de uma canula orogástrica. A implanta o ocorreu na mucosa previamente lesionada por clampeadura da parede, nas regi es da jun o es fago-gástrica, pequena curvatura e grande curvatura. O crescimento tumoral ocorreu em todos os animais e, embora na jun o es fago-gástrica a incidência de pega tenha sido de apenas 20%, possivelmente em fun o do epitélio queratinizado, na pequena curvatura a incidência foi de 80% e na grande curvatura todos os animais apresentaram tumor. Esses dados demonstraram que é possível implantar o tumor em 100% dos animais inoculados, o que comprova a exequibilidade da técnica. A vantagem dessa metodologia sobre as outras já descritas na literatura, também usando o carcinossarcoma 256 de Walker, é que o tumor cresce a partir da mucosa, reproduzindo as condi es de desenvolvimento do tumor gástrico espontaneo. A média de sobrevida dos animais inoculados é de 13,2±1,98 dias. Além disso, como o tumor se desenvolve em todos os animais inoculados, pode-se dispensar métodos radiológicos e ultra-sonográficos para evidenciar a sua presen a. Trata-se, portanto, de um método simples e eficaz e que possibilita a implanta o do tumor em um grande número de animais, facilitando, inclusive, o estudo do tumor em diversas fases do seu desenvolvimento, testes de novos fármacos e esquemas terapêuticos.
An Application of the Taguchi Method (Robust Design) to Environmental Engineering: Evaluating Advanced Oxidative Processes in Polyester-Resin Wastewater Treatment  [PDF]
Messias Borges Silva, Livia Melo Carneiro, João Paulo Alves Silva, Ivy dos Santos Oliveira, Hélcio José Izário Filho, Carlos Roberto de Oliveira Almeida
American Journal of Analytical Chemistry (AJAC) , 2014, DOI: 10.4236/ajac.2014.513092
Abstract: This paper presents the Taguchi Method, a statistical design modelling for experiments applied in environmental engineering. This method was applied to optimize the treatment conditions of polyester-resin effluent by means of Advanced Oxidative Processes (AOPs) using chemical oxygen demand (COD) as response parameter. The influence of each independent parameter including respective interactions was evaluated by Taguchi Method, which allowed determining the most statistically significant variables and conditions to best fit the process. Results showed that Taguchi Method is a very useful tool for environmental engineering field and possible simplifications of analysis and calculations through commercially available software.
Avalia o do efeito do Risedronato Sódico na consolida o de fraturas: estudo experimental em ratos
Oliveira Luiz Antonio Alcantara de,Guarniero Roberto,Rodrigues Consuelo Junqueira,Santana Paulo José de
Acta Ortopédica Brasileira , 2004,
Abstract: Realizou-se um estudo experimental com 40 ratos da ra a Lewis visando-se avaliar a influência do risedronato sódico na consolida o de fraturas em animais submetidos à dieta aprotéica e dieta protéica, divididos aleatoriamente em quatro grupos, com 10 animais em cada grupo, assim constituídos: grupo I, com dieta protéica, sem risedronato (grupo controle); grupo II, dieta protéica, com risedronato; grupo III, dieta aprotéica, sem risedrionato; grupo IV, dieta aprotéica, com risedronato. Os ratos foram submetidos a fraturas semelhantes, no 15o dia e à eutanásia no 43o dia do experimento. As variáveis analisadas incluíram a evolu o ponderal, avalia o radiográfica, densitometria óssea, avalia o histomorfométrica do calo ósseo, dosagens sanguíneas de cálcio, fósforo, fosfatase alcalina, proteínas totais, albumina e osteocalcina. Concluiu-se que o risedronato exerceu influência positiva no processo de consolida o de fraturas em ratos nutridos e desnutridos, e aumentou a densidade mineral óssea. O risedronato ocasionou a forma o de tecido ósseo maduro de melhor qualidade e morfologia.
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