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Search Results: 1 - 10 of 235222 matches for " Paulo C.;Kuchembuck "
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Metabolismo oxidativo dos neutrófilos de ovinos tratados com monensina sódica e experimentalmente submetidos à acidose ruminal
Afonso José Augusto B.,Ciarlini Paulo C.,Kuchembuck Márcio R.G.,Kohayagawa Aguemi
Pesquisa Veterinária Brasileira , 2002,
Abstract: A acidose láctica ruminal é causada pela ingest o excessiva de carboidratos de fermenta o rápida sem uma prévia adapta o dos mocroorganismos, podendo com isso gerar distúrbios metabólicos graves aos ruminantes. Este trabalho tem por objetivo estudar o metabolismo oxidativo dos neutrófilos em ovinos tratados com a monensina sódica na acidose láctica ruminal induzida experimentalmente. Foram empregados 18 ovinos, machos, mesti os (Ideal x Merino), fistulados no rúmen; dos quais nove receberam 33 mg/kg da dieta do ionóforo ao dia, durante 30 dias, os demais ovinos pertenceram ao grupo controle. A acidose foi induzida fornecendo 15g de sacarose/kg de peso corporal. A avalia o clínica e as amostras de rúmen e sangüíneas foram obtidas antes (momento controle) 0h e às 6h, 12h, 24h e 48h pós-indu o. Em ambos os grupos os animais apresentaram manifesta es clínicas de acidose láctica ruminal 6 horas pós-indu o. A partir deste período se observou uma diminui o significativa (p<0,05) do pH no fluido ruminal, que alcan ou valores inferiores a cinco, e diferen as significativas (p<0,05) entre os grupos a partir das 12h pós-indu o foram constatadas, nos quais os ovinos tratados com a monensina apresentaram valores superiores em rela o aos do grupo controle. Durante este período ocorreu uma inibi o do metabolismo oxidativo dos neutrófilos nos animais estudados, ocorrendo restabelecimento desta fun o somente nos ovinos que receberam a monensina. O pH sangüíneo, a glicose plasmática e o cálcio ionizável sofreram altera es nos seus índices. A concentra o do cortisol sérico elevou-se de forma significativa (p< 0,05) em ambos os grupos, porém diferen as (p< 0,05) entre eles foram constatadas ao final no período de observa o. O tratamento com a monensina n o influenciou o metabolismo oxidativo dos neutrófilos, inibido pela acidose láctica; porém se verificou uma recupera o mais rápida deste metabolismo nos animais em que receberam o ionóforo.
Metabolismo oxidativo dos neutrófilos de ovinos tratados com monensina sódica e experimentalmente submetidos à acidose ruminal
Afonso, José Augusto B.;Ciarlini, Paulo C.;Kuchembuck, Márcio R.G.;Kohayagawa, Aguemi;Feltrin, Lúcia P.Z.;Ciarlini, Luciana D.R. Pinoti;Laposy, Cecília B.;Mendon?a, Carla L.;Takahira, Regina K.;
Pesquisa Veterinária Brasileira , 2002, DOI: 10.1590/S0100-736X2002000400001
Abstract: ruminal acidosis is due to excessive ingestion of carbohydrates of rapid fermentation without previous adaptation of the microorganisms, causing severe metabolic disturbances to the animals. the objective of the present study was to assess the neutrophilic oxidative metabolism in sheep treated with sodium monensin in experimentally induced ruminal lactic acidosis. a total of 18 male sheep, half-bred (ideal x merino), fistulated in the rumen, were used; nine of them received 33 mg/kg of the ionophore diet per day, for 30 days; the others were controls. the acidosis was induced by supplying 15g of sucrose/kg of body weight. the clinical evaluation and the rumen and blood samples were obtained before (0h) and at 6, 12, 24 and 48 hours post-induction. in both groups, all the animals presented clinical manifestations of ruminal lactic acidosis 6 hours after the induction. from this period on, a significant ph decrease (p<0.05) was observed in the ruminal fluid, which reached levels below 5. there were relevant differences (p<0.05) between the groups 12 hours after the induction, when the sheep treated with monensin had higher values than those of the control group. during this period, the oxidative metabolism of the neutrophils remained inhibited, and the reestablishment of this function only occurred in the sheep which received monensin. blood ph, plasmatic glucose and the ionizable calcium suffered alterations within its levels. the seric cortisol concentration rose significantly (p<0.05) in both groups, although differences (p<0.05) between them were found at the end of the observation period. the treatment with monensin did not influence the oxidative metabolism of the neutrophils inhibited by the lactic acidosis; however, a faster recovery of this metabolism was verified in the animals treated with the ionophore.
Mieloencefalite protozoária eqüina (Relato de caso).
A. P. C. Peixoto,M. R. G. Kuchembuck,R. C. Gon?alves,S. B. Chiacchio
Revista Brasileira de Saúde e Produ??o Animal , 2005,
Abstract: RESUMO :O objetivo deste trabalho foi relatar um caso de mieloencefalite protozo ria eq ina no Estado de S £o Paulo, Brasil. O diagn 3stico se baseou nos sinais cl -nicos, no resultado positivo para anticorpos contra Sarcocystis neurona no soro e no l -quor pela t cnica de Western blot. Palavras chave: Mieloencefalite, Sarcocystis neurona, eq inos. SUMMARY: The purpose of this work was to present a case of equine protozoal myeloencephalitis (EPM) in the State of S £o Paulo, Brazil. The diagnosis was based on the clinical sings, the positive results of the serum and cerebrospinal fluid analysis (Western blot) for antibodies against Sarcocystis neurona. Keywords: Myeloencephalitis, Sarcocystis neurona,
Diagnóstico sorológico da brucelose bovina em animais adultos vacinados com dose reduzida da cepa 19 de Brucella abortus
Jardim, Gustavo Coelho;Pires, Pedro Paulo;Mathias, Luis Antonio;Ribeiro, Olímpio Crisóstomo;Kuchembuck, Márcio Rubens Graf;
Pesquisa Veterinária Brasileira , 2006, DOI: 10.1590/S0100-736X2006000300009
Abstract: the study evaluated the use of a reduced dose of the brucella abortus strain 19 vaccine, in an adult herd negative for the disease, by serological diagnostic techniques, advocated by the brazilian program for animal brucellosis and tuberculosis control and eradication, and by an indirect elisa. the complement fixation test detecteed 46.77% positives, the rose bengal test 67.74%, the mercaptoethanol with standard agglutination test 87.09% and the elisa id 100%. the reduced dose influenced the serological diagnosis. none of the techniques reached a suitable specificity for use in the herd under those conditions, up to 3 months after vaccination.
DIFERENCIA??O CLíNICA DA BRONCOPNEUMONIA MODERADA E GRAVE EM BEZERROS
Gon?alves, Roberto Calderon;Kuchembuck, Márcio Rubens Graf;Curi, Paulo Roberto;Chiacchio, Simone Biagio;Almeida, Clóvis Teixeira de;Borges, Alexandre Secorun;
Ciência Rural , 2001, DOI: 10.1590/S0103-84782001000200012
Abstract: to evaluate the main clinical signs of bronchopneumonia in calves and determie the classification as moderate or severe form, 48 calves were divided in 3 groups: 10 clinically healthy animals (g1), 18 animals with moderate bronchopneumonia (g2) and 20 animals with severe bronchopneumonia (g3). the classification of these experimental groups was based on clinical characterization and confirmed by statistical group analysis. in the clinically healthy calves (g1), the results revealed absence of clinical signs of respiratory disease and low clinical score. the calves with bronchopneumonia were identified by the association of clinical signs showing pulmonary abnormalities. cough and positive cough reflex, high corporal temperature and cardiac rate, abnormal tracheobronchial and bronchobronchiolar noises and interrupted inspiration were important in the bronchopneumonia diagnosis but did not differentiated the severe or moderate cases. high respiratory rate, dispnoea, signs of compact parenchyma, silence areas and crackles and whizzels were the most important signs to differentiate the clinical cases of bronchopneumonia.
Lavagem traqueobr?nquica por sondagem nasotraqueal em bezerros
Gon?alves, R.C.;Mattos, M.C.F.I.;Kuchembuck, M.R.G.;Lopes, R.S.;Borges, A.S.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2004, DOI: 10.1590/S0102-09352004000300004
Abstract: tracheobronchial lavage through nasotracheal via was performed in 10 clinically health calves. they were maintained in lateral recumbence to perform the procedure. a small tube inserted into a guide tube was introduced until the tracheal bifurcation, producing cough, facilitating the collection of the lavage fluid. the mean number of total cells present in the samples was 133,750 cells/ml. the differential counting was represented by 77.2% of macrophages, 14.9% of cylindrical epithelial cells, 6.0% of neutrophils, 1.8% of lymphocytes. the cylindrical ciliated cells represented 79.0% of the sample and the nonciliated cells represented 21.0%. the mean number of macrophages was 78.5 of binucleated cells, 20.5 of trinucleated cells, and 28.5 of giant cells per smear. the tracheobronchial lavage obtained by this technique was an efficient method to characterize the cytological population of the lungs of clinically health calves.
Eccentric Binary Millisecond Pulsars
Paulo C. C. Freire
Physics , 2009,
Abstract: In this paper we review the recent discovery of several millisecond pulsars (MSPs) in eccentric binary systems. Timing these MSPs we were able to estimate (and in one case precisely measure) their masses. These results suggest that, as a class, MSPs have a much wider range of masses (1.3 to > 2 solar masses) than the normal and mildly recycled pulsars found in double neutron star (DNS) systems (1.25 < Mp < 1.44 solar masses). This is very likely to be due to the prolonged accretion episode that is thought to be required to form a MSP. The likely existence of massive MSPs makes them a powerful probe for understanding the behavior of matter at densities larger than that of the atomic nucleus; in particular, the precise measurement of the mass of PSR J1903+0327 ($1.67 +/- 0.01 solar masses) excludes several "soft" equations of state for dense matter.
The Pulsar Population in Globular Clusters and in the Galaxy
Paulo C. C. Freire
Physics , 2012, DOI: 10.1017/S1743921312023770
Abstract: In this paper, I review some of the basic properties of the pulsar population in globular clusters (GCs) and compare it with the the Galactic disk population. The neutron stars (NSs) in GCs were likely formed - and appear to continue forming - in highly symmetric supernovae (SNe), likely from accretion-induced collapse (AIC). I review the many pulsar finds and discuss some particularly well populated GCs and why they are so. I then discuss some particularly interesting objects, like millisecond pulsars (MSPs) with eccentric orbits, which were heavily perturbed by passing stars. Some of these systems, like NGC 1851A and NGC 6544B, are almost certainly the result of exchange interactions, i.e., they are witnesses to the very same processes that created the large population of MSPs in the first place. I also review briefly the problem posed by the presence of young pulsars in GCs (with a special emphasis on a sub-class of young pulsars, the super-energetic MSPs), which suggest continuing formation of NSs in low-velocity SNe. In the final section, I discuss the possibility of an analogous population in the Galaxy and highlight a particularly interesting case, PSR J1903+0327, where the primary neutron star appears to have formed with a small-velocity kick and small fractional mass loss. Systems with primary NSs formed in electron-capture SNe should constitute a distinct low-velocity Galactic population akin in many respects to the GC population. Current high-resolution surveys of the Galactic plane should be able to detect it clearly.
Análise de crescimento do amendoinzeiro (Arachis hypogaea L.) com rela??o à infesta??o de pragas
Castro, Paulo R. C.;
Anais da Escola Superior de Agricultura Luiz de Queiroz , 1974, DOI: 10.1590/S0071-12761974000100014
Abstract: the peanut culture is one of the most important of our country. however it is studied with very little intensity. the thrips enneothrips flavens moulton, 1941, the leaf beetle diabrotica speciosa (germar, 1824) and the red necked peanut worm stegasta bosquella (chambers, 1875), are insect pests often found in peanuts (arachis hypogaea l.), in jaboticabal, state of s?o paulo, brazil. the main proposal of this work is to study the variation of leaf area index, leaf area ratio, net assimilation rate and relative growth rate in an experiment in which one of the treatments consisted in the application of methyl parathion at 0,09% (e.c. 60%) and the other was a check in which no pesticide was applied. the results obtained during the plant life cycle were confronted. the increments of leaf area index and leaf area ratio were observed to be similar for both treatments, but both parameters were found to be practically always smaller for the check treatment. finally, it was shown that the net assimilation rate and the relative growth rate was almost always slightly superior in the plot that received no treatment.
A??o de fitoreguladores nos teores de macronutrientes em tomateiro (Lycopersicon esculentum Mill.)
Castro, Paulo R. C.;
Anais da Escola Superior de Agricultura Luiz de Queiroz , 1978, DOI: 10.1590/S0071-12761978000100001
Abstract: this research deals with the effects of exogenous growth regulators on mineral nutrition of the tomato plant (lycopersicon esculentum mill, cv. 'miguel pereira'). to study the influence on mineral nutrition, (2-chloroethyl) trimethilammonium chloride (ccc), at concentration of 2,000 ppm, succinic acid-2,2-dimethylhydrazide (sadh) (4,000 ppm), gibberellic acid (ga) (100 ppm), (2-chloroethyl) phosphonic acid (cepa) (200 ppm), indole-3-acetic acid (iaa) (100 ppm), and 6-furfurylamino purine (fap) (500 ppm) were applied. higher levels of nitrogen, calcium and magnesium occurred in the stem of plants sprayed with ccc. treatments with fap. sadh and cepa demonstrated an increase in nitrogen levels in the stem. cepa also increased calcium contents in stems. growth regulators used did not alter the levels of macronutrients in leaves of tomatoes in relation to check plants.
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