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Search Results: 1 - 10 of 219676 matches for " Paula L. Marcet "
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Authentication scheme for routine verification of genetically similar laboratory colonies: a trial with Anopheles gambiae
Elien E Wilkins, Paula L Marcet, Alice C Sutcliffe, Paul I Howell
BMC Biotechnology , 2009, DOI: 10.1186/1472-6750-9-91
Abstract: We present a method for establishing authentication matrices to routinely distinguish and confirm that laboratory strains have not become physically or genetically mixed through contamination events in the laboratory. We show a specific example with application to Anopheles gambiae sensu stricto strains at the Malaria Research and Reference Reagent Resource Center. This authentication matrix is essentially a series of tests yielding a strain-specific combination of results.These matrix-based methodologies are useful for several mosquito and insect populations but must be specifically tailored and altered for each laboratory based on the potential contaminants available at any given time. The desired resulting authentication plan would utilize the least amount of routine effort possible while ensuring the integrity of the strains.Experimental research often relies on the use of genetically similar animals to ensure that results can be generalized and are repeatable. A research animal's value, or its "uniqueness", consists of its known genotype and phenotype. Inadvertent contamination could call into question the validity of research conducted with the same strain over the course of time: genotypic changes can create different phenotypic properties [1,2]. Thus, research results reliant on the genotypic or phenotypic nature of the strain could be invalidated.Concurrent maintenance of multiple genetically similar laboratory strains requires much attention to detail along with a strict program for guaranteeing that they are reared without contamination. Even with meticulous handling and rearing practices, the possibility of inadvertent contamination still exists [3]. Researchers often consider contamination events only after deviations in research results occur [4,5]. However, by the time laboratory strains are found to be contaminated, research time and effort have already been consumed. Thus, using routinely authenticated strains with a known genetic pedigree is worthw
Hidden Sylvatic Foci of the Main Vector of Chagas Disease Triatoma infestans: Threats to the Vector Elimination Campaign?
Leonardo A. Ceballos equal contributor,Romina V. Piccinali equal contributor,Paula L. Marcet equal contributor,Gonzalo M. Vazquez-Prokopec equal contributor,M. Victoria Cardinal equal contributor,Judith Schachter-Broide,Jean-Pierre Dujardin,Ellen M. Dotson,Uriel Kitron,Ricardo E. Gürtler
PLOS Neglected Tropical Diseases , 2011, DOI: 10.1371/journal.pntd.0001365
Abstract: Background Establishing the sources of reinfestation after residual insecticide spraying is crucial for vector elimination programs. Triatoma infestans, traditionally considered to be limited to domestic or peridomestic (abbreviated as D/PD) habitats throughout most of its range, is the target of an elimination program that has achieved limited success in the Gran Chaco region in South America. Methodology/Principal Findings During a two-year period we conducted semi-annual searches for triatomine bugs in every D/PD site and surrounding sylvatic habitats after full-coverage spraying of pyrethroid insecticides of all houses in a well-defined rural area in northwestern Argentina. We found six low-density sylvatic foci with 24 T. infestans in fallen or standing trees located 110–2,300 m from the nearest house or infested D/PD site detected after insecticide spraying, when house infestations were rare. Analysis of two mitochondrial gene fragments of 20 sylvatic specimens confirmed their species identity as T. infestans and showed that their composite haplotypes were the same as or closely related to D/PD haplotypes. Population studies with 10 polymorphic microsatellite loci and wing geometric morphometry consistently indicated the occurrence of unrestricted gene flow between local D/PD and sylvatic populations. Mitochondrial DNA and microsatellite sibship analyses in the most abundant sylvatic colony revealed descendents from five different females. Spatial analysis showed a significant association between two sylvatic foci and the nearest D/PD bug population found before insecticide spraying. Conclusions Our study shows that, despite of its high degree of domesticity, T. infestans has sylvatic colonies with normal chromatic characters (not melanic morphs) highly connected to D/PD conspecifics in the Argentinean Chaco. Sylvatic habitats may provide a transient or permanent refuge after control interventions, and function as sources for D/PD reinfestation. The occurrence of sylvatic foci of T. infestans in the Gran Chaco may pose additional threats to ongoing vector elimination efforts.
Phylogeographic Pattern and Extensive Mitochondrial DNA Divergence Disclose a Species Complex within the Chagas Disease Vector Triatoma dimidiata
Fernando A. Monteiro, Tatiana Peretolchina, Cristiano Lazoski, Kecia Harris, Ellen M. Dotson, Fernando Abad-Franch, Elsa Tamayo, Pamela M. Pennington, Carlota Monroy, Celia Cordon-Rosales, Paz Maria Salazar-Schettino, Andrés Gómez-Palacio, Mario J. Grijalva, Charles B. Beard, Paula L. Marcet
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0070974
Abstract: Background Triatoma dimidiata is among the main vectors of Chagas disease in Latin America. However, and despite important advances, there is no consensus about the taxonomic status of phenotypically divergent T. dimidiata populations, which in most recent papers are regarded as subspecies. Methodology and Findings A total of 126 cyt b sequences (621 bp long) were produced for specimens from across the species range. Forty-seven selected specimens representing the main cyt b clades observed (after a preliminary phylogenetic analysis) were also sequenced for an ND4 fragment (554 bp long) and concatenated with their respective cyt b sequences to produce a combined data set totalling 1175 bp/individual. Bayesian and Maximum-Likelihood phylogenetic analyses of both data sets (cyt b, and cyt b+ND4) disclosed four strongly divergent (all pairwise Kimura 2-parameter distances >0.08), monophyletic groups: Group I occurs from Southern Mexico through Central America into Colombia, with Ecuadorian specimens resembling Nicaraguan material; Group II includes samples from Western-Southwestern Mexico; Group III comprises specimens from the Yucatán peninsula; and Group IV consists of sylvatic samples from Belize. The closely-related, yet formally recognized species T. hegneri from the island of Cozumel falls within the divergence range of the T. dimidiata populations studied. Conclusions We propose that Groups I–IV, as well as T. hegneri, should be regarded as separate species. In the Petén of Guatemala, representatives of Groups I, II, and III occur in sympatry; the absence of haplotypes with intermediate genetic distances, as shown by multimodal mismatch distribution plots, clearly indicates that reproductive barriers actively promote within-group cohesion. Some sylvatic specimens from Belize belong to a different species – likely the basal lineage of the T. dimidiata complex, originated ~8.25 Mya. The evidence presented here strongly supports the proposition that T. dimidiata is a complex of five cryptic species (Groups I–IV plus T. hegneri) that play different roles as vectors of Chagas disease in the region.
Canaliculite: relato de caso e conduta
Carneiro, Rachel Camargo;Macedo, Erick Marcet Santiago de;Oliveira, Patrícia Paula Deboni Greenhalgh de;
Arquivos Brasileiros de Oftalmologia , 2008, DOI: 10.1590/S0004-27492008000100023
Abstract: canaliculitis is a rare infection of the lacrimal canaliculus with actinomyces israelii as its most common etiologic agent. the objective of this paper is to report a rare pathology, as well as its clinical and surgical management. female patient, 44 years old, presenting clinical manifestations compatible with canaliculitis, was submitted to surgical treatment preserving the lacrimal punctum. intubation was performed into the lacrimal channel with silicone tube for three months, associated with periodical pre-programmed bathings with antibiotic, in order to avoid complications and recurrence. this case confirms the literature reports in which the surgical treatment is more effective than the conservative treatment.
Phylogenetic analysis of homologous fatty acid synthase and polyketide synthase involved in aflatoxin biosynthesis
Marina Marcet-Houben,Maria Cabré,José L. Paternáin,Antoni Romeu
Bioinformation , 2008,
Abstract: The first two steps of aflatoxin biosynthesis are catalyzed by the HexA/B and by the Pks protein. The phylogenetic analysis clearly distinguished fungal HexA/B from FAS subunits and from other homologous proteins. The phylogenetic trees of the HexA and HexB set of proteins share the same clustering. Proteins involved in the synthesis of fatty acids or in the aflatoxin or sterigmatocystin biosynthesis cluster separately. The Pks phylogenetic tree also differentiates the aflatoxin-related polypeptide sequences from those of other kinds of secondary metabolism. The function of some of the A. flavus Pks homologues may be deduced from the phylogenetic analysis. The conserved sequence motifs of protein domains shared by HexA/B and Pks - namely, β-polyketide synthase (KS), acetyl transferase (AT) and acyl carrier protein (ACP) - have been identified, and the HexA/B and Pks involved in aflatoxin biosynthesis have been distinguished from those involved in primary metabolism or other kinds of secondary metabolism.
Rad51 ATP binding but not hydrolysis is required to recruit Rad10 in synthesis-dependent strand annealing sites in S. cerevisiae  [PDF]
Justin Karlin, Paula L. Fischhaber
Advances in Biological Chemistry (ABC) , 2013, DOI: 10.4236/abc.2013.33033

Several modes of eukaryotic of DNA double strand break repair (DSBR) depend on synapsis of complementary DNA. The Rad51 ATPase, the S. cerevisiae homolog of E. coli RecA, plays a key role in this process by catalyzing homology searching and strand exchange between an invading DNA strand and a repair template (e.g. sister chromatid or homologous chromosome). Synthesis dependent strand annealing (SDSA), a mode of DSBR, requires Rad51. Another repair enzyme, the Rad1-Rad10 endonuclease, acts in the final stages of SDSA, hydrolyzing 3 overhanging single-stranded DNA. Here we show in vivo by fluo-rescence microscopy that the ATP binding function of yeast Rad51 is required to recruit Rad10 SDSA sites indicating that Rad51 pre-synaptic filament formation must occur prior to the recruitment of Rad1-Rad10. Our data also show that Rad51 ATPase activity, an important step in Rad51 filament disassembly, is not absolutely required in order to recruit Rad1- Rad10 to DSB sites.

A rela??o entre qualifica??o profissional e a reinser??o produtiva: o caso dos bancários do Banestado
Nascimento, Zinara Marcet Andrade;
Educar em Revista , 2006, DOI: 10.1590/S0104-40602006000100016
Abstract: this text presents the process of reintegration of former banestado bank employees to the labour market after a wave of voluntary lay-offs which took place during the late 1990's. its main objective is to analyze whether or not such a reintegration was made easier by the level of professional qualification acquired at the bank. interviews were conducted with the former employees in order to assess the degree of professional qualification attained by those employees during their employment years. the interviews were carried out in curitiba and metropolitan region. the results reveal a surprising degree of reintegration in the labour market. such a reintegration, however, carries with it all the problems of the present day precariousness of the labour market. another important result is the conviction of the interviewees as to their decision: none of them to regret about their decision to leave the bank. as a consequence of this, we judged necessary to introduce information on the contruction process of the bank and its experiences of professional qualification in such an organization. the main conclusions are that professional qualification indeed facilitates reintegration of the employees to the labor market. moreover, the voluntary lay-off programs were successful in the process of downsizing of the bank as well as a means to diminish labour resistance, especially among senior employees.
Primeres Jornades sobre toponímia catalana
Jaume Marcet i Prims
Llengua & Literatura , 2004, DOI: 10.2436/l&l.vi.1134
Incremento de la sensibilidad analítica del sistema FasciDIG para el diagnóstico de Fasciola hepatica Increase of analytical sensitivity of FasciDIG system for the diagnosis of Fasciola hepatica
Ricardo Marcet Sánchez,Mabel Figueredo Pino,Fidel A Nú?ez Fernández,Lázara Rojas Rivero
Revista Cubana de Medicina Tropical , 2012,
Abstract: Introducción: la fasciolosis en Cuba es una enfermedad enzoótica en el ganado y en los últimos a os existe un incremento en el número de casos en humanos. El diagnóstico coproparasitológico de la fasciolosis es poco sensible y laborioso, por lo que es importante el uso de los métodos inmuno-enzimáticos sobre todo aquellos que son capaces de detectar antígenos de este parásito en las heces. En el Instituto de Medicina Tropical "Pedro Kourí" se cuenta con un sistema de detección de antígenos denominado FasciDIG con una sensibilidad de 10 ng/mL. Objetivo: aumentar la sensibilidad del FasciDIG realizando modificaciones a este método diagnóstico. Métodos: en un sistema simulado se evaluaron FasciDIG y FasciDIG modificado, utilizando diluciones seriadas dobles de antígenos a concentraciones desde 1 000 ng/mL hasta 1,95 ng/mL. El FasciDIG se modificó utilizando como segundo anticuerpo el obtenido en conejos contra antígenos de excreción-secreción de Fasciola hepatica conjugado con biotina seguido de la adición de un conjugado extravidina-peroxidasa comercial. Las heces se colectaron del recto de 96 reses destinadas para sacrificio y se evaluaron por ambos métodos. Se calculó el índice de concordancia Kappa entre ambos sistemas. Resultados: el límite de detección obtenido para el FasciDIG fue de 3,9 ng/mL mientras que el FasciDIG modificado detectó hasta 1,95 ng/mL. El índice de concordancia calculado entre los dos ensayos fue de 0,6238, que corresponde a un índice de acuerdo sustancial o bueno. Conclusiones: el método FasciDIG modificado resultó tener una mayor sensibilidad analítica que el FasciDIG y puede ser un complemento para el diagnóstico de la fasciolosis. Se debe incrementar el número de muestras y determinar la sensibilidad y especificidad utilizando la técnica de sedimentación copa-cónica seriada como regla de oro. Introduction: fasciolosis is an endemic disease in cattle in Cuba and there is an increase in the number of reported human cases in recent years. The copro-parasitological diagnosis of fasciolosis has low sensitivity and is hard-working; for that reason, it is important to use immunoenzymatic methods mainly those that can detect this parasite antigens in the feces. A system for antigen detection called FasciDIG , with a reported sensitivity of 10 ng/mL has been developed in "Pedro Kouri" Institute of Tropical Medicine. Objective: to increase the sensitivity of FasciDIG through some modifications to this diagnostic method. Methods: two foul dilutions (concentrations of antigen 1 000 ng/mL- 1.95ng/mL in H2O Tween-20) were evaluated in
Replace Psychometric Inferences with Direct Brain Measurements: LORETA Reflects Traditional Cerebral Loci for Neuropsychological Tests  [PDF]
Paula L. Corradini, Michael A. Persinger
Neuroscience & Medicine (NM) , 2015, DOI: 10.4236/nm.2015.63018
Abstract: Inferences of subtle cerebral injury and dysfunction have been historically dependent upon psychometric tests from which clinical neuropsychological profiles are generated. In addition to being secondary, over-inclusive and crude indicators of cerebral activity, psychometric tests are subject to economic incentives to “re-norm” traditional methods under the pretense of “ensuring” contemporary representations that are sanctioned by regulating organizations dominated by agendas of control over the interpretations of clinicians. The validity of neuropsychological tests is essential for their perspicacious application and interpretations. We measured the quantitative electroen-cephalographic profiles and calculated s-LORETA (standardized Low Resolution Electromagnetic Tomography) profiles in real time for normal men and women while they engaged in both traditional and novel neuropsychological tests that were employed to infer localized brain injury. Conspicuous alterations in source current density within specific frequency bands occurred within various regions of the right prefrontal region during performance of the Category, Design Fluency and Conditioned Spatial Association Test, the prefrontal medial surface during Toe Graphaesthesia, the caudal medial surface during Toe Gnosis, the left temporal region during Speech-Sounds, and within the right retrosplenial-parahippocampal region for Seashore Rhythms. Results supported the well established regional associations with the classic neuropsychological tests, verified the cerebral localization with more recent procedures, and emphasized the utility of modern real-time, direct cerebral imaging procedures.
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