Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99


Any time

2020 ( 69 )

2019 ( 699 )

2018 ( 823 )

2017 ( 840 )

Custom range...

Search Results: 1 - 10 of 452843 matches for " Paul B. S. Lai "
All listed articles are free for downloading (OA Articles)
Page 1 /452843
Display every page Item
Histone Deacetylase Inhibitors Facilitate Dihydroartemisinin-Induced Apoptosis in Liver Cancer In Vitro and In Vivo
Chris Zhiyi Zhang, Yinghua Pan, Yun Cao, Paul B. S. Lai, Lili Liu, George Gong Chen, Jingping Yun
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0039870
Abstract: Liver cancer ranks in prevalence and mortality among top five cancers worldwide. Accumulating interests have been focused in developing new strategies for liver cancer treatment. We have previously showed that dihydroartemisinin (DHA) exhibited antitumor activity towards liver cancer. In this study, we demonstrated that histone deacetylase inhibitors (HDACi) significantly augmented the antineoplastic effect of DHA via increasing apoptosis in vitro and in vivo. Inhibition of ERK phosphorylation contributed to DHA-induced apoptosis, due to the fact that inhibitor of ERK phosphorylation (PD98059) increased DHA-induced apoptosis. Compared with DHA alone, the combined treatment with DHA and HDACi reduced mitochondria membrane potential, released cytochrome c into cytoplasm, increased p53 and Bak, decreased Mcl-1 and p-ERK, activated caspase 3 and PARP, and induced apoptotic cells. Furthermore, we showed that HDACi pretreatment facilitated DHA-induced apoptosis. In Hep G2-xenograft carrying nude mice, the intraperitoneal injection of DHA and SAHA resulted in significant inhibition of xenograft tumors. Results of TUNEL and H&E staining showed more apoptosis induced by combined treatment. Immunohistochemistry data revealed the activation of PARP, and the decrease of Ki-67, p-ERK and Mcl-1. Taken together, our data suggest that the combination of HDACi and DHA offers an antitumor effect on liver cancer, and this combination treatment should be considered as a promising strategy for chemotherapy.
Identification and Characterization of Tropomyosin 3 Associated with Granulin-Epithelin Precursor in Human Hepatocellular Carcinoma
Ching Yan Lam, Chi Wai Yip, Terence C. W. Poon, Christine K. C. Cheng, Eddy W. Y. Ng, Nicholas C. L. Wong, Phyllis F. Y. Cheung, Paul B. S. Lai, Irene O. L. Ng, Sheung Tat Fan, Siu Tim Cheung
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0040324
Abstract: Background and Aim Granulin-epithelin precursor (GEP) has previously been reported to control cancer growth, invasion, chemo-resistance, and served as novel therapeutic target for cancer treatment. However, the nature and characteristics of GEP interacting partner remain unclear. The present study aims to identify and characterize the novel predominant interacting partner of GEP using co-immunoprecipitation and mass spectrometry. Methods and Results Specific anti-GEP monoclonal antibody was used to capture GEP and its interacting partner from the protein extract of the liver cancer cells Hep3B. The precipitated proteins were analyzed by SDS-PAGE, followed by mass spectrometry and the protein identity was demonstrated to be tropomyosin 3 (TPM3). The interaction has been validated in additional cell models using anti-TPM3 antibody and immunoblot to confirm GEP as the interacting partner. GEP and TPM3 expressions were then examined by real-time quantitative RT-PCR in clinical samples, and their transcript levels were significantly correlated. Elevated TPM3 levels were observed in liver cancer compared with the adjacent non-tumorous liver, and patients with elevated TPM3 levels were shown to have poor recurrence-free survival. Protein expression of GEP and TPM3 was observed only in the cytoplasm of liver cancer cells by immunohistochemical staining. Conclusions TPM3 is an interacting partner of GEP and may play an important role in hepatocarcinogenesis.
Heritability of Cardiovascular and Personality Traits in 6,148 Sardinians
Giuseppe Pilia equal contributor ?,Wei-Min Chen equal contributor,Angelo Scuteri,Marco Orrú,Giuseppe Albai,Mariano Dei,Sandra Lai,Gianluca Usala,Monica Lai,Paola Loi,Cinzia Mameli,Loredana Vacca,Manila Deiana,Nazario Olla,Marco Masala,Antonio Cao,Samer S Najjar,Antonio Terracciano,Timur Nedorezov,Alexei Sharov,Alan B Zonderman,Gon?alo R Abecasis ,Paul Costa,Edward Lakatta,David Schlessinger
PLOS Genetics , 2006, DOI: 10.1371/journal.pgen.0020132
Abstract: In family studies, phenotypic similarities between relatives yield information on the overall contribution of genes to trait variation. Large samples are important for these family studies, especially when comparing heritability between subgroups such as young and old, or males and females. We recruited a cohort of 6,148 participants, aged 14–102 y, from four clustered towns in Sardinia. The cohort includes 34,469 relative pairs. To extract genetic information, we implemented software for variance components heritability analysis, designed to handle large pedigrees, analyze multiple traits simultaneously, and model heterogeneity. Here, we report heritability analyses for 98 quantitative traits, focusing on facets of personality and cardiovascular function. We also summarize results of bivariate analyses for all pairs of traits and of heterogeneity analyses for each trait. We found a significant genetic component for every trait. On average, genetic effects explained 40% of the variance for 38 blood tests, 51% for five anthropometric measures, 25% for 20 measures of cardiovascular function, and 19% for 35 personality traits. Four traits showed significant evidence for an X-linked component. Bivariate analyses suggested overlapping genetic determinants for many traits, including multiple personality facets and several traits related to the metabolic syndrome; but we found no evidence for shared genetic determinants that might underlie the reported association of some personality traits and cardiovascular risk factors. Models allowing for heterogeneity suggested that, in this cohort, the genetic variance was typically larger in females and in younger individuals, but interesting exceptions were observed. For example, narrow heritability of blood pressure was approximately 26% in individuals more than 42 y old, but only approximately 8% in younger individuals. Despite the heterogeneity in effect sizes, the same loci appear to contribute to variance in young and old, and in males and females. In summary, we find significant evidence for heritability of many medically important traits, including cardiovascular function and personality. Evidence for heterogeneity by age and sex suggests that models allowing for these differences will be important in mapping quantitative traits.
Spectral variations of the X-ray binary pulsar LMC X-4 during its long period intensity variation and a comparison with Her X-1
S. Naik,B. Paul
Physics , 2003, DOI: 10.1051/0004-6361:20030111
Abstract: We present spectral variations of the binary X-ray pulsar LMC X-4 using the RXTE/PCA observations at different phases of its 30.5 day long super-orbital period. Only out of eclipse data were used for this study. During the high state of the super-orbital period of LMC X-4, the spectrum is well described by a high energy cut-off power-law with a photon index in the range of 0.7-1.0 and an iron emission line. In the low state, the spectrum is found to be flatter with power-law photon index in the range 0.5-0.7. A direct correlation is detected between the continuum flux in 7-25 keV energy band and the iron emission line flux. The equivalent width of the iron emission line is found to be highly variable during low intensity state, whereas it remains almost constant during the high intensity state of the super-orbital period. It is observed that the spectral variations in LMC X-4 are similar to those of Her X-1 (using RXTE/PCA data). These results suggest that the geometry of the region where the iron line is produced and its visibility with respect to the phase of the super-orbital period is similar in LMC X-4 and Her X-1. A remarkable difference between these two systems is a highly variable absorption column density with phase of the super-orbital period that is observed in Her X-1 but not in LMC X-4.
Spectral properties of the X-ray binary pulsar LMC X-4 during different intensity states
S. Naik,B. Paul
Physics , 2002, DOI: 10.1007/BF02702461
Abstract: We present spectral variations of the binary X-ray pulsar LMC X-4 observed with the RXTE/PCA during different phases of its 30.5 day long third period. Only out of eclipse data were used for this study. The 3-25 keV spectrum, modeled with high energy cut-off power-law and iron line emission is found to show strong dependence on the intensity state. Correlations between the Fe line emission flux and different parameters of the continuum are presented here.
Discontiguous States of America: The Paradox of Unincorporation in Craig Santos Perez’s Poetics of Chamorro Guam
Paul Lai
Journal of Transnational American Studies , 2011,
Abstract: Eclipsed by other islands incorporated into the United States after the Spanish-American War of 1898, Guam has nevertheless played a crucial role in the development of the American Pacific as a strategic military site. Like other territories of the United States, Guam’s ambiguous legal status and the presence of native peoples, cultures, and histories signal the paradox of unincorporated territories that troubles the issues of belonging and identification as “American.” This essay takes up poet-scholar Craig Santos Perez’s work to assert the primacy of Indigenous Chamorro histories, languages, and cultures in understanding the island’s place in and out of the American Empire. Perez’s experimental, decolonial poetics fracture narratives of America as a benevolent force in the Pacific; of English as the only relevant language of the Mariana Islands and America; of Spanish and Catholic domination as a relic of the past; of environmental transformations wrought by the intimacies of empire; and of simplistic accounts of assimilation or resistance to militarization and colonialism. Furthermore, by foregrounding “Discontiguous States of America” as an organizing trope for comparative understanding of unincorporated territories in the Caribbean and Pacific, American Indian reservation spaces on the continent, and the outlying states of Alaska and Hawai‘i, this essay argues that transnational American Studies must look within its territorial possessions to Indigenous sovereignty claims as well as outside to global flows in order to offer a truly critical, transnational American Studies.
On the viability of some Emergent Universe models
S. Ghose,B. C. Paul
Physics , 2011,
Abstract: A particular class of flat Emergent Universe scenario is studied in light of recent observational data. Observationally permissible ranges of values are obtained for the model parameters. The class of model studied here can accommodate different composition of matter-energy as cosmic fluid. It is found that recent observations favour some compositions over others while some compositions can be ruled out with some level of confidence.
Inflaton Field and Primordial Blackhole
B. C. Paul,S. Chakraborty
Physics , 2001, DOI: 10.1142/S0218271802002293
Abstract: Primordial black hole formation has been studied using an inflaton field with a variable cosmological term as the potential.
Iodine speciation in rain, snow and aerosols and possible transfer of organically bound iodine species from aerosol to droplet phases
B. S. Gilfedder,S. Lai,M. Petri,H. Biester
Atmospheric Chemistry and Physics Discussions , 2008,
Abstract: Iodine oxides, such as iodate, should theoretically be the only stable sink species for iodine in the troposphere. However, field observations have increasingly found very little iodate and significant amounts of iodide and organically bound iodine in precipitation and aerosols. The aim of this study was to investigate iodine speciation, including the organic fraction, in rain, snow, and aerosols in an attempt to further clarify aqueous phase iodine chemistry. Diurnal aerosol samples were taken with a 5 stage cascade impactor and a virtual impactor (PM2.5) from the Mace Head research station, Ireland, during summer 2006. Rain was collected from Australia, New Zealand, Patagonia, Germany, Ireland, and Switzerland while snow was obtained from Greenland, Germany, Switzerland, and New Zealand. All samples were analysed for total iodine by inductively coupled plasma mass spectrometry (ICP-MS) and speciation was determined by coupling an ion chromatography unit to the ICP-MS. Total iodine in the aerosols from Mace Head gave a median concentration of 50 pmol m 3 of which the majority was associated with the organic fraction (median day: 91±7%, night: 94±6% of total iodine). Iodide exhibited higher concentrations than iodate (median 5% vs. 0.8% of total iodine), and displayed significant enrichment during the day compared to the night. Interestingly, up to 5 additional, presumably anionic organic peaks were observed in all IC-ICP-MS chromatograms, composing up to 15% of the total iodine. Organically bound iodine was also the dominant fraction in all rain and snow samples, with lesser amounts of iodide and iodate (iodate was particularly low in snow). Two of the same unidentified peaks found in aerosols were also observed in precipitation from both Southern and Northern Hemispheres, suggesting that these species are transferred from the aerosol phase into precipitation. It is suggested that organo-I is formed by reactions between HOI and organic matter derived from the ocean surface layer. This may then photolytically decompose to give iodide and the unidentified species. The data in this study show that iodine oxides are the least abundant species in rain, snow, and aerosols and therefore considerably more effort is required on aqueous phase iodine chemistry for a holistic understanding of the iodine cycle.
Can Changes in Soil Properties in Organic Banana Production Suppress Fusarium Wilt?  [PDF]
Paul Geense, Anthony B. Pattison, Tegan L. Kukulies, Johannes M. S. Scholberg, Agustin B. Molina
Natural Resources (NR) , 2015, DOI: 10.4236/nr.2015.63017
Abstract: Organic agriculture promotes disease suppression through healthy soils by increasing biological activity and diversity through the application of organic fertilizers and increasing organic inputs. Fusarium wilt of bananas (Fusarium oxysporum f. sp. Fcubense) (Foc), also known as Panama disease, has been a devastating disease throughout the world. So far, no fungicides or cultural measures have been found that control Foc sufficiently. The aim of this research was to assess whether organic-based farming systems were more resilient than inorganic farming systems to soil borne diseases, in particular Fusarium wilts. A survey was conducted comparing five organic and five conventional banana plantations at paired sites in north Queensland, Australia. Soil samples were collected and analysed for chemical, physical and biological soil health indicators. Disease development of F. oxysporum f. sp. lycopersici in tomatoes and Foc in bananas were studied in pot trials to pursue clues for identifying Fusarium suppressive soil traits. Organic soils from the survey showed higher microbial activity and lower disease symptom expression (both with tomatoes and bananas) than conventional soils. In the survey, nematode diversity and soil sulphate content were recurring indicators in all experiments showing close correlations to pathogen growth, disease expression and plant health. Organic soils were lower in plant-parasitic nematodes and sulphate sulphur levels and higher in nematode diversity, labile soil C and microbial indicators. Soil conduciveness or suppression of Foc appeared to be largely governed by competition for carbon. Measurement of soil microbial enzyme activity, nematode community structure and diversity and possibly sulphate sulphur seem to provide a relatively reliable indicator for general disease suppression. Differences between organic and conventional agriculture cannot be related to single management practices, but may be linked to synergies among system components.
Page 1 /452843
Display every page Item

Copyright © 2008-2017 Open Access Library. All rights reserved.