oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2019 ( 392 )

2018 ( 541 )

2017 ( 555 )

2016 ( 799 )

Custom range...

Search Results: 1 - 10 of 340559 matches for " Patricia Sáez "
All listed articles are free for downloading (OA Articles)
Page 1 /340559
Display every page Item
Proteomic Analysis through Adventitious Rooting of Pinus radiata Stem Cuttings with Different Rooting Capabilities  [PDF]
Carolina álvarez, Luis Valledor, Patricia Sáez, Manuel Sánchez-Olate, Darcy Ríos
American Journal of Plant Sciences (AJPS) , 2016, DOI: 10.4236/ajps.2016.714174
Abstract: In forest production systems, vegetative propagation of elite clones through adventitious rooting is a common practice. In Chile, adventitious rooting is the main methodology for vegetative reproduction of Pinus radiata. However, the capability of produce adventitious roots in gymnosperms decreases with aging. While it is true that some efforts have been made to identify markers or/and regulators of the aging process and adventitious rooting, molecular mechanisms that regulate both processes are scarcely known, especially at protein level. This research evaluated qualitative and quantitative changes in protein accumulation during the adventitious rooting process of P. radiata stem cuttings, with different rooting capabilities. Beside, an analysis of morpho-anatomical changes was performed in stem cuttings with high and low rooting capabilities, during the adventitious rooting process. It was observed that juvenile 1-year-old stem cuttings rooted in a 100%, while aged stem cuttings (3-year-old) presented only a 20% of rooting. According to the results of differential protein accumulation, univariate and multivariate analysis indicated that in total, 114 and 89 proteins were differentially accumulated in juvenile and aged cuttings, respectively. Also, identification of such proteins showed the presence of proteins related to cell wall organization and the presence of a protein related with proper distribution of auxin PIN transporter, both key in the new meristem formation process during adventitious rooting.
Fraccionamiento y recuperación de proteínas solubles libres de fenoles en estacas de lenga (Nothofagus pumilio)
Latsague Vidal,Mirtha; Sáez Delgado,Patricia;
Bosque (Valdivia) , 2006, DOI: 10.4067/S0717-92002006000300006
Abstract: at present, there is no information about nothofagus fractioning and protein recovery from cutting crude extracts; necessary condition to, in vitro, demonstrate enzymatic activity with cofactors presence. besides, this information is crucial to determine enzymatic expression levels, which in turn could allow to know physiological aspects of lenga (nothofagus pumilio), cuttings which could be conditioning the poor results obtained in rooting treatments. to obtain information about what was pointed out above, a protocol which not only allows to separate phenol free proteins from crude extracts of lenga cuttings, but to determine its composition as well, was validated and standardized. from each of the 60 samples, 36 fractions were analyzed, obtaining, repetitively, phenol free proteins from fraction 14 on. the results obtained were statistically validated and show that the method employed allows to fractionate and to recover phenol free proteins from lenga cuttings through a sephadex g-50 column (p < 0.05).
Fraccionamiento y recuperación de proteínas solubles libres de fenoles en estacas de lenga (Nothofagus pumilio) Fractioning and soluble Protein free Phenols recovery from lenga (Nothofagus pumilio) cuttings
Mirtha Latsague Vidal,Patricia Sáez Delgado
Bosque (Valdivia) , 2006,
Abstract: Para Nothofagus spp. no existe a la fecha información acerca del fraccionamiento y recuperación de proteínas a partir de extracto crudo de estacas, condición necesaria para demostrar in vitro actividad enzimática en presencia de cofactores. Además, esta información es crucial para determinar los niveles de expresión enzimática, lo que permitiría conocer aspectos fisiológicos de las estacas de lenga (Nothofagus pumilio) que podrían estar condicionando la baja respuesta obtenida en los tratamientos de enraizamiento. Para obtener información acerca de lo se alado se validó y estandarizó un protocolo de fraccionamiento, el cual permitió separar proteínas libres de fenoles a partir de extracto crudo de estacas de lenga, como asimismo determinar su contenido. De cada una de las 60 muestras se analizaron 36 fracciones. A partir de la fracción 14 en adelante se obtuvieron proteínas libres de fenoles. Los resultados obtenidos se validaron estadísticamente y mostraron que la técnica permite fraccionar y recuperar proteínas libres de fenoles a través de columna Sephadex G-50 en estacas de lenga (P < 0,05). At present, there is no information about Nothofagus fractioning and protein recovery from cutting crude extracts; necessary condition to, in vitro, demonstrate enzymatic activity with cofactors presence. Besides, this information is crucial to determine enzymatic expression levels, which in turn could allow to know physiological aspects of lenga (Nothofagus pumilio), cuttings which could be conditioning the poor results obtained in rooting treatments. To obtain information about what was pointed out above, a protocol which not only allows to separate phenol free proteins from crude extracts of lenga cuttings, but to determine its composition as well, was validated and standardized. From each of the 60 samples, 36 fractions were analyzed, obtaining, repetitively, phenol free proteins from fraction 14 on. The results obtained were statistically validated and show that the method employed allows to fractionate and to recover phenol free proteins from lenga cuttings through a Sephadex G-50 column (P < 0.05).
Effect of Photon Flux Density and Exogenous Sucrose on the Photosynthetic Performance during In Vitro Culture of Castanea sativa  [PDF]
Patricia L. Sáez, León A. Bravo, Manuel Sánchez-Olate, Paulina B. Bravo, Darcy G. Ríos
American Journal of Plant Sciences (AJPS) , 2016, DOI: 10.4236/ajps.2016.714187
Abstract: The low photon flux density (PFD) under in vitro conditions and sucrose added to the culture medium negatively limits the photochemical activity and photoprotective mechanisms of microshoots. In this work we hypothesize that decreasing sucrose in the culture medium in combination with increasing irradiance, could improve the photosynthesis and consequently the in vitro growth. We evaluated the effect of exogenous sucrose (30 and 5 g·L-1, HS and LS, respectively), under different PFD (50 and 150 μmol photons m-2·s-1, LL and HL, respectively) on the photosynthetic performance and growth of Castanea sativa microshoots. Decreasing sucrose negatively affected the physiological attributes evaluated. Only chloroplast ultrastructure was improved by LS; however this did not lead to an improved in photosynthesis or growth. HL HS produced an increase in photosynthetic activity and chlorophyll contents, reaching under these conditions a higher proliferation rate and biomass production. Additionally, the photochemical activity (electron transport rate and non-photochemical quenching) was improved by HL. Thus, our results suggest that, at least for C. sativa HL is beneficial during the in vitro culture, improving photosynthetic performance as well as growth, but this is only possible in the presence of moderate concentrations of sucrose added to the culture medium.
Tratamientos pregerminativos para Myrceugenia exsucca (Myrtaceae)
Latsague Vidal,Mirtha; Sáez Delgado,Patricia; Coronado Ancaten,Leandra;
Bosque (Valdivia) , 2010, DOI: 10.4067/S0717-92002010000300008
Abstract: the knowledge of specific germination requirement is important in plants production using seeds. the majority of the native species need of cultural management, among them those related with appropriate conditions to the germination of their seeds. however, available information to develop this methodology in species of myrtaceae is still conspicuously lacking, as is the case of myrceugenia exsucca (pitra). currently, pitra is the most abundant species in the forested wetlands of the araucanía region, thus reports of results that contribute to its propagation are important in the conservation of these ecosystems. the objective of the present work was to evaluate the effect of different pregermination treatments (imbibition, cold stratification and gibberellic acid) on viable seeds of m. exsucca through tests of viability and germination under laboratory conditions. the seeds were treated with five treatments: soak in distilled water for 24 h; soak in 250 mg l-1 gibberellic acid for 12 h; soak in 250 mg l-1 gibberellic acid during 24 h; cold stratification at 5 °c during 15 days. percentage of germination and index of germination velocity were determined. we found 90 % of viability in our experiments. the results showed that the best results were obtained soaking the seeds in distilled water during 24 h with an average germination percentage of 71 %. this information might be required for the management and conservation of m. exsucca.
Actividad de AIA oxidasa en estacas de especies del género Nothofagus
Latsague,Mirtha; Sáez,Patricia; Paredes,Marco; Alarcón,Edith;
Bosque (Valdivia) , 2007, DOI: 10.4067/S0717-92002007000200006
Abstract: there is variation in rooting capacity among the different species of nothofagus. lenga cuttings (n. pumilio) present an almost null answer to rooting, compared to raulí (n. alpina) and roble (n. obliqua). according to antecedents compiled in literature, the endogenous levels of indole acetic acid (iaa), hormone responsible for the formation of adventitious roots, vary depending on the levels of iaa oxidase activity. based on these observations, the iaa oxidase activity was comparativily analyzed in cuttings from these three nothofagus species. to quantify the enzymatic activity, a spectrophotometric test was carried out, measuring at 535 nm. the residual amount of iaa was determined by reference to a calibration curve. the enzymatic activity was expressed in iaa mm mg protein-1 hours-1. the results show that lenga presents a greater iaa oxidase enzymatic activity when compared to the other analyzed species (p < 0.05). these results would support at least partially the differences in the rootting capacity in nothofagus species studys.
Inducción de enraizamiento en estacas de Berberidopsis corallina con ácido indolbutírico
Latsague Vidal,Mirtha; Sáez Delgado,Patricia; Hauenstein Barra,Enrique;
Bosque (Valdivia) , 2008, DOI: 10.4067/S0717-92002008000300006
Abstract: berberidopsis corallina is an endemic species from chile, presenting conservation problems and being catalogued as “endangered” species. its persistent diminution is associated to cutting and competition with eucalyptus spp. and pinus radiata. the objective of the present work was to contribute in the management for the recovery of this species through the evaluation of its reproductive capacity by means of propagation by cuttings. for vegetative propagation, semi-woody cuttings were collected during march of 2007 in villa las araucarias, region of la araucanía. the collected cuttings were treated with indolbutyric acid (iba) at different concentrations (0, 500, 1,000 and 1,500 mgl-1) as rooting stimulator. after five months of maintenance of cuttings in a bottom heating, an 87% of total rooting was obtained. the best result concerning the process of rizogenesis was shown by 1,000 mg l-1 iba with a 90% of rooting. besides, the highest average of root length was found in this same treatment with an average of 13.6 cm. the greatest number of roots was obtained in 1,500 mgl-1 treatment with a value average of 38.1 roots by cuttings. it is concludes that b. corallina can be vegetatively reproduced through the rizogenesis of cuttings treated with iba, as a practice to contribute to the recovery of this species.
Tratamientos pregerminativos para Myrceugenia exsucca (Myrtaceae) Pregermination treatments of Myrceugenia exsucca (Myrtaceae)
Mirtha Latsague Vidal,Patricia Sáez Delgado,Leandra Coronado Ancaten
Bosque (Valdivia) , 2010,
Abstract: El conocimiento de los tratamientos pregerminativos específicos resulta de interés en la producción de plantas a través de semillas. La mayoría de las especies nativas necesitan de manejos culturales, entre ellos, los relacionados con las condiciones apropiadas para la germinación de sus semillas. Hasta el momento, especificaciones precisas para el desarrollo de estas metodologías en especies de la familia Myrtaceae son escasas, tal es el caso de Myrceugenia exsucca (pitra), especie más abundante en los humedales boscosos de la región de La Araucanía. Así, informar resultados que contribuyan a su propagación por semillas resulta importante en la conservación de este tipo de ecosistemas. El objetivo de este estudio fue evaluar viabilidad y tratamientos pregerminativos en semillas de M. exsucca a través de ensayos de germinación en condiciones de laboratorio. Las semillas fueron tratadas con cuatro tratamientos: remojo con agua destilada por 24 h; remojo en ácido giberélico, 250 mg L-1 por 12 h; remojo en ácido giberélico, 250 mg L-1 por 24 h y estratificación fría a 5 °C por 15 días. Para cada tratamiento se utilizaron cinco repeticiones de 25 semillas cada uno. Se determinó porcentaje de germinación e índice de velocidad de germinación. Como resultado de la prueba de tetrazolium, se obtuvo 90 % de viabilidad. El remojo en agua destilada por 24 h como tratamiento pregerminativo aumenta el porcentaje de germinación, alcanzando 71 %. Esta información podría ser requerida para el manejo y conservación de M. exsucca. The knowledge of specific germination requirement is important in plants production using seeds. The majority of the native species need of cultural management, among them those related with appropriate conditions to the germination of their seeds. However, available information to develop this methodology in species of Myrtaceae is still conspicuously lacking, as is the case of Myrceugenia exsucca (pitra). Currently, pitra is the most abundant species in the forested wetlands of the Araucanía Region, thus reports of results that contribute to its propagation are important in the conservation of these ecosystems. The objective of the present work was to evaluate the effect of different pregermination treatments (imbibition, cold stratification and gibberellic acid) on viable seeds of M. exsucca through tests of viability and germination under laboratory conditions. The seeds were treated with five treatments: soak in distilled water for 24 h; soak in 250 mg L-1 gibberellic acid for 12 h; soak in 250 mg L-1 gibberellic acid during 24 h; cold stra
Inducción de enraizamiento en estacas de Berberidopsis corallina con ácido indolbutírico Induction of rooting in cuttings of Berberidopsis corallina with indol butyric acid
Mirtha Latsague Vidal,Patricia Sáez Delgado,Enrique Hauenstein Barra
Bosque (Valdivia) , 2008,
Abstract: Berberidopsis corallina es una especie endémica de Chile que debido a su estado de conservación ha sido catalogada como especie “en peligro de extinción”. Su persistente disminución ha estado asociada a tala y competencia con Eucalyptus spp. y Pinus radiata. El objetivo del presente trabajo fue contribuir en la gestión de recuperación de esta especie a través de la evaluación de su capacidad reproductiva mediante propagación por estacas. Para la propagación vegetativa se colectaron estacas semile osas durante marzo de 2007 en el sector Villa Las Araucarias, Región de La Araucanía. Las estacas cosechadas se trataron con ácido indolbutírico (AIB) en distintas concentraciones (0, 500, 1.000 y 1.500 mg L-1) como factor de enraizamiento. Después de cinco meses de mantenidas las estacas en cama caliente se obtuvo un 87% de enraizamiento total. La concentración de AIB de 1.000 mg L-1 mostró los mejores resultados respecto al proceso de rizogénesis con un 90% de enraizamiento. Además se encontró el mayor promedio de longitud de raíces en este mismo tratamiento con un promedio de 13,64 cm. El mayor número de raíces se obtuvo en el tratamiento 1.500 mg L-1 con un valor promedio de 38,11 raíces por estaca. Se concluye que B. corallina puede ser reproducido vegetativamente a través de la rizogénesis de estacas tratadas con AIB, como una práctica para contribuir a la recuperación de esta especie. Berberidopsis corallina is an endemic species from Chile, presenting conservation problems and being catalogued as “endangered” species. Its persistent diminution is associated to cutting and competition with Eucalyptus spp. and Pinus radiata. The objective of the present work was to contribute in the management for the recovery of this species through the evaluation of its reproductive capacity by means of propagation by cuttings. For vegetative propagation, semi-woody cuttings were collected during March of 2007 in Villa Las Araucarias, Region of La Araucanía. The collected cuttings were treated with Indolbutyric acid (IBA) at different concentrations (0, 500, 1,000 and 1,500 mgL-1) as rooting stimulator. After five months of maintenance of cuttings in a bottom heating, an 87% of total rooting was obtained. The best result concerning the process of rizogenesis was shown by 1,000 mg L-1 IBA with a 90% of rooting. Besides, the highest average of root length was found in this same treatment with an average of 13.6 cm. The greatest number of roots was obtained in 1,500 mgL-1 treatment with a value average of 38.1 roots by cuttings. It is concludes that B. corallina can be veg
Actividad de AIA oxidasa en estacas de especies del género Nothofagus IAA oxidase activity on species of Nothofagus genus cuttings
Mirtha Latsague,Patricia Sáez,Marco Paredes,Edith Alarcón
Bosque (Valdivia) , 2007,
Abstract: Entre las distintas especies de Nothofagus existe variación en la capacidad de enraizamiento. Las estacas de lenga (N. pumilio) presentan una respuesta casi nula al enraizamiento, comparada con raulí (N. alpina) y roble (N. obliqua). Según antecedentes recopilados en literatura, los niveles endógenos de ácido indol acético (AIA), hormona responsable de la formación de raíces adventicias, varían según los niveles de actividad de AIA oxidasa (AIA-O). Basados en estas observaciones, la actividad de AIA-O fue comparativamente analizada en estacas de estas tres especies de Nothofagus. Para cuantificar la actividad enzimática se realizó un ensayo de espectrofotometría, midiendo a 535 nm λ. La cantidad residual de AIA se estimó por referencia a una curva de calibración. La actividad enzimática fue expresada en mM de AIA mg de proteína-1 h-1. Los resultados muestran que N. pumilio presenta una mayor actividad enzimática de AIA oxidasa en comparación con las otras especies analizadas (P < 0,05). Estos resultados respaldarían, al menos parcialmente, las diferencias en la capacidad de rizogénesis en las especies de Nothofagus estudiadas. There is variation in rooting capacity among the different species of Nothofagus. Lenga cuttings (N. pumilio) present an almost null answer to rooting, compared to raulí (N. alpina) and roble (N. obliqua). According to antecedents compiled in literature, the endogenous levels of indole acetic acid (IAA), hormone responsible for the formation of adventitious roots, vary depending on the levels of IAA oxidase activity. Based on these observations, the IAA oxidase activity was comparativily analyzed in cuttings from these three Nothofagus species. To quantify the enzymatic activity, a spectrophotometric test was carried out, measuring at 535 nm. The residual amount of IAA was determined by reference to a calibration curve. The enzymatic activity was expressed in IAA mM mg protein-1 hours-1. The results show that lenga presents a greater IAA oxidase enzymatic activity when compared to the other analyzed species (P < 0.05). These results would support at least partially the differences in the rootting capacity in Nothofagus species studys.
Page 1 /340559
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.