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Search Results: 1 - 10 of 3926 matches for " Paragonimus sp "
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Inmunodiagnóstico en Didelphis marsupialis usando un antígeno de Paragonimus de Venezuela
Gómez Martínez,Erika; Díaz-Bello,Zoraida; Zavala-Jaspe,Reinaldo; Tulio Díaz,Marcos; Noya,Oscar; Alarcón de Noya,Belkisyole;
Acta bioqu?-mica cl?-nica latinoamericana , 2010,
Abstract: paragonimus are trematodes that normally live in the lungs of carnivorous and omnivorous mammals such as humans. an outbreak of paragonimus sp. in which didelphis marsupialis was the only wild reservoir incriminated was described in eastern venezuela. in order to have immunological tools to detect the presence of paragonimus sp. in this reservoir, a whole antigen of the adult worm of this parasite was elaborated. didelphis marsupialis were captured in the locality of aguas blancas, montes municipality, sucre state, venezuela, from which blood samples were obtained and a search for worms was performed in lungs. worms were homogenized and ultracentrifugated to obtain fspa to perform immunoassay (elisa) to detect antibodies in opossums. the electrophoresis analysis showed a pattern of 22 molecules between 6 and 82 kda; by western blot, the antigenic recognition of 8 antigenic molecules appeared,112 kda and 268 kda molecules being the most strongly recognized by positive sera. the negative sera did not recognize any band. the production of igy in chicken enabled the development of reagents capable of performing a standard immunodiagnosis technique to find specific anti-paragonimus sp. in didelphis marsupialis in order to establish epidemiological surveillance of these reservoirs in endemic areas.
Diversidad enzimática de la fracción soluble de un aislado venezolano de adultos Paragonimus sp
Gómez Martínez,Erika; Ballén,Diana; Tulio Díaz,Marcos; Díaz Bello,Zoraida; Zavala-Jaspe,Reinaldo; Alarcón de Noya,Belkisyolé; Noya,Oscar; Cesari,Italo M;
Boletín de Malariología y Salud Ambiental , 2010,
Abstract: paragonimus sp. is a trematode that causes chronic inflammation of the lung in carnivorous mammals and humans, which constitute a public health problem in asian and latin american countries. trematodes have enzymes that facilitate their penetration and migration through different host organs to ensure their life cycle. to evaluate the enzymatic diversity of the soluble fraction (fspa, 100,000 g) of a venezuelan isolate of paragonimus sp. adult worms, several enzyme determinations were conducted at different ph. the activities of enzymes releasing p-nitrophenol or p-nitroanilina from the corresponding dye-related synthetic peptides were assessed by interpolating absorbance (a 405 nm) values in the corresponding calibration curve (a 405 nm vs. nmol); on the other hand, absorbances of 2-naphtylamine and 2-naphtols released from another series of synthetic substrates were read at different wavelengths between 450 nm and 620 nm to assess for the activity of the corresponding hydrolases. phosphohydrolase, glycosidase and peptidase activities were detected in fspa, β-n-acetyl-β-d-glucosaminidase (0.55 μmol/h/mg, ph 5.5) and cystein protease (0.4 μmol/h/mg, ph 5.5) being higher than all the other detected activities. these activities are probably related to the adult worm habitat and its need for glycan and peptide degradation of lung secretions. these results represent the first enzymatic study done with a venezuelan isolate of adult paragonimus sp. worms collected from the common reservoir didelphis marsupialis.
Paragonimosis in the Cajabamba and Condebamba districts, Cajamarca, Peru
CORNEJO, William;HUIZA, Alina;ESPINOZA, Yrma;ALVA, Pilar;SEVILLA, Carlos;CENTURION, Wilson;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2000, DOI: 10.1590/S0036-46652000000500002
Abstract: stool samples from 409 pre-school and school students, living in six villages of the cajabamba and condebamba districts, cajamarca, perú, were examined using wet preparations and lumbreras' method, looking for paragonimus eggs. fecal and sputum samples from two children (0.5%) of 6 and 8 year-old showed eggs of paragonimus. one hundred and twenty freshwater crabs, hypolobocera chilensis eigenmanni, were collected from the condebamba valley and 21 (17.5%) of them were infected with p. mexicanus (syn. p. peruvianus) metacercariae. our results show the persistence of paragonimus in human beings and in the main source of infection, the crabs.
Paragonimosis in the Cajabamba and Condebamba districts, Cajamarca, Peru
CORNEJO William,HUIZA Alina,ESPINOZA Yrma,ALVA Pilar
Revista do Instituto de Medicina Tropical de S?o Paulo , 2000,
Abstract: Stool samples from 409 pre-school and school students, living in six villages of the Cajabamba and Condebamba districts, Cajamarca, Perú, were examined using wet preparations and Lumbreras' method, looking for Paragonimus eggs. Fecal and sputum samples from two children (0.5%) of 6 and 8 year-old showed eggs of Paragonimus. One hundred and twenty freshwater crabs, Hypolobocera chilensis eigenmanni, were collected from the Condebamba valley and 21 (17.5%) of them were infected with P. mexicanus (syn. P. peruvianus) metacercariae. Our results show the persistence of Paragonimus in human beings and in the main source of infection, the crabs.
Inmunodiagnóstico en Didelphis marsupialis usando un antígeno de Paragonimus de Venezuela Immunodiagnostic in Didelphis marsupialis using an antigen of Venezuelan Paragonimus
Erika Gómez Martínez,Zoraida Díaz-Bello,Reinaldo Zavala-Jaspe,Marcos Tulio Díaz
Acta bioqu?-mica cl?-nica latinoamericana , 2010,
Abstract: Los Paragonimus son trematodos que habitualmente viven en los pulmones de mamíferos carnívoros y omnívoros, entre ellos el hombre. En el oriente venezolano se encuentra el único foco de Paragonimus sp. donde Didelphis marsupialis es el único reservorio demostrado hasta ahora. Con el fin de tener herramientas de inmunodiagnóstico que detecten la presencia de Paragonimus sp. en esta especie, se elaboraron varios reactivos para realizar un ensayo inmunoenzimático ELISA. Entre ellos se obtuvo un antígeno crudo soluble de vermes adultos de Paragonimus y una inmunoglobulina de gallina anti-IgG de Didelphis marsupialis. Los mismos se capturaron en la localidad de Aguas Blancas, municipio Montes, estado Sucre, Venezuela, y se obtuvieron muestras sanguíneas; en el caso de estar infectados, los vermes adultos se extrajeron del pulmón. Los parásitos se homogenizaron y ultracentrifugaron para obtener la fracción soluble del parásito (FSPA) como antígeno para el ELISA y Western blot y detectar los anticuerpos en los Didelphis marsupialis. El análisis electroforético mostró 22 moléculas entre 6 y 82 kDa; por Western blot se presentó un reconocimiento antigénico de 8 moléculas siendo las de 112 kDa y 268 kDa las más reconocidas por los sueros positivos. Los sueros negativos no reconocieron ninguna proteína. La producción de IgY en gallinas permitió desarrollar las técnicas de inmunodiagnóstico para la búsqueda de anticuerpos específicos anti-Paragonimus sp. en Didelphis, cuya aplicación permitirá establecer la vigilancia epidemiológica de estos reservorios en áreas endémicas sin sacrificio de los mismos. Paragonimus are trematodes that normally live in the lungs of carnivorous and omnivorous mammals such as humans. An outbreak of Paragonimus sp. in which Didelphis marsupialis was the only wild reservoir incriminated was described in eastern Venezuela. In order to have immunological tools to detect the presence of Paragonimus sp. in this reservoir, a whole antigen of the adult worm of this parasite was elaborated. Didelphis marsupialis were captured in the locality of Aguas Blancas, Montes municipality, Sucre state, Venezuela, from which blood samples were obtained and a search for worms was performed in lungs. Worms were homogenized and ultracentrifugated to obtain FSPA to perform immunoassay (ELISA) to detect antibodies in opossums. The electrophoresis analysis showed a pattern of 22 molecules between 6 and 82 kDa; by western blot, the antigenic recognition of 8 antigenic molecules appeared,112 kDa and 268 kDa molecules being the most strongly recognized by positive
Paragonimus sp. in crabs and awareness of the educational community to aquatic ecosystems in La Miel and La Clara, Caldas, Antioquia Paragonimus sp. en cangrejos y sensibilización de la comunidad educativa hacia los ecosistemas acuáticos de La Miel y La Clara, Caldas, Antioquia
Sandra Milena Arias,Lina Marcela Salazar,Eudoro Casas,Alexandra Henao
Biomédica , 2011,
Abstract: Introduction. Previous research has demonstrated the presence of Paragonimus spp. in crabs of three municipalities located in the Medellín river basin. To design a plan for the control and prevention of paragonimosis, parasite presence must be demonstrated in the upper area of the river, and an awareness campaign of its risks must involve the participation of the educational community in focusing attention on the local aquatic resources. Objective. The presence of Paragonimus spp. metarcercariae was established in freshwater crabs from La Miel and La Clara streams, and an awareness campaign created public awareness to management of the aquatic ecosystems for risk reduction. Materials and methods. Nine crabs were collected from streams of La Miel and La Clara villages in 2007 and 2008. The crabs were indentified, sacrificed in the laboratory and examined for presence of Paragonimus spp. metacercariae. Five workshops were conducted with students of educational institutions of the two villages to improve the student perception of paragonimosis risks associated with the local streams. Results. The crabs were identified as Hypolobocera bouvieri. One crab was found with Paragonimus spp. in La Miel. A mollusk, Aroapyrgus colombiensis, another host of the parasite was found in La Clara. That aquatic ecosystems are a great attraction for the local was well established. Conclusions. Foci of Paragonimus spp. flatworms are present in the upper area of Medellín river which includes Caldas. However, local school children were uninformed about the risk of paragonimosis associated with the local aquatic ecosystem. Therefore this province must be included in programs for prevention and control of paragonimosis. The prevalence of this disease recommends educational programs that promote awareness of local aquatic ecosystems and their parasitic inhabitants. Introducción. Estudios previos demostraron la presencia de Paragonimus sp. en cangrejos de tres municipios en la cuenca del río Medellín. Para dise ar un plan para el control y la prevención de la paragonimosis, es necesario determinar la presencia del parásito en la parte alta del río, con la participación de la comunidad educativa, para dirigir su atención hacia el recurso hídrico. Objetivo. Establecer la presencia de metacercarias de Paragonimus sp. en cangrejos de las quebradas La Miel y La Clara, y sensibilizar a la comunidad educativa hacia el manejo adecuado de sus ecosistemas acuáticos. Materiales y métodos. En 2007 y 2008 se recolectaron cangrejos de quebradas de las veredas La Miel y La Clara. En el laboratori
LA PARAGONIMOSIS EN ESCOLARES DEL VALLE DE CONDEBAMBA, CAJAMARCA-PERU
CORNEJO,WILLIAM; NAQUIRA,CESAR; ESPINOZA,YRMA; HUIZA,ALINA; SEVILLA,CARLOS;
Parasitología al día , 1998, DOI: 10.4067/S0716-07201998000300008
Abstract: paragonimosis in school children from condebamba valley, cajamarca-peru a study of the prevalence of paragonimosis among primary students has been carried on three villages of condebamba valley, department of cajamarca, by using the skin test and the examination of stools. from 77 skin tests, 2 were positive (2.6%), which were also positive by counter immunoelectrophoresis. all 77 stool samples were negative for paragonimus eggs. these results showed a declination of human paragonimosis cases in condebamba valley.
LA PARAGONIMOSIS EN ESCOLARES DEL VALLE DE CONDEBAMBA, CAJAMARCA-PERU
WILLIAM CORNEJO,CESAR NAQUIRA,YRMA ESPINOZA,ALINA HUIZA
Parasitología al día , 1998,
Abstract: RESUMEN Se realizó un estudio de la prevalencia de paragonimosis en escolares de educación primaria de tres localidades del valle de Condebamba, Departamento de Cajamarca, utilizando la intradermorreacción y el examen de heces. De las 77 intradermorreacciones practicadas 2 fueron positivas (2,6%), las cuales también fueron positivas por contrainmuno-electroforesis. Todas las 77 muestras de heces examinadas fueron negativas a huevos de Paragonimus. Estos resultados muestran una declinación de casos de paragonimosis humana en el valle de Condebamba PARAGONIMOSIS IN SCHOOL CHILDREN FROM CONDEBAMBA VALLEY, CAJAMARCA-PERU A study of the prevalence of paragonimosis among primary students has been carried on three villages of Condebamba valley, Department of Cajamarca, by using the skin test and the examination of stools. From 77 skin tests, 2 were positive (2.6%), which were also positive by counter immunoelectrophoresis. All 77 stool samples were negative for Paragonimus eggs. These results showed a declination of human paragonimosis cases in Condebamba valley.
Paragonimus sp. en cangrejos y sensibilización de la comunidad educativa hacia los ecosistemas acuáticos de La Miel y La Clara, Caldas, Antioquia
Arias,Sandra Milena; Salazar,Lina Marcela; Casas,Eudoro; Henao,Alexandra; Velásquez,Luz Elena;
Biomédica , 2011,
Abstract: introduction. previous research has demonstrated the presence of paragonimus spp. in crabs of three municipalities located in the medellín river basin. to design a plan for the control and prevention of paragonimosis, parasite presence must be demonstrated in the upper area of the river, and an awareness campaign of its risks must involve the participation of the educational community in focusing attention on the local aquatic resources. objective. the presence of paragonimus spp. metarcercariae was established in freshwater crabs from la miel and la clara streams, and an awareness campaign created public awareness to management of the aquatic ecosystems for risk reduction. materials and methods. nine crabs were collected from streams of la miel and la clara villages in 2007 and 2008. the crabs were indentified, sacrificed in the laboratory and examined for presence of paragonimus spp. metacercariae. five workshops were conducted with students of educational institutions of the two villages to improve the student perception of paragonimosis risks associated with the local streams. results. the crabs were identified as hypolobocera bouvieri. one crab was found with paragonimus spp. in la miel. a mollusk, aroapyrgus colombiensis, another host of the parasite was found in la clara. that aquatic ecosystems are a great attraction for the local was well established. conclusions. foci of paragonimus spp. flatworms are present in the upper area of medellín river which includes caldas. however, local school children were uninformed about the risk of paragonimosis associated with the local aquatic ecosystem. therefore this province must be included in programs for prevention and control of paragonimosis. the prevalence of this disease recommends educational programs that promote awareness of local aquatic ecosystems and their parasitic inhabitants.
Estudio de foco de paragonimosis en Fuente Clara, Robledo, área periurbana de Medellín, Antioquia
Casas,Eudoro; Gómez,Catalina; Valencia,Erika; Salazar,Laura; Velásquez,Luz Elena;
Biomédica , 2008,
Abstract: introduction. human paragonimosis in colombia was assumed to be restricted to the sylvatic areas. however, in 2005, crabs infected with paragonimus were found in fuente clara, an urban sector in medellín. objective. a study was designed to understand the ecoepidemiology of paragonimosis. programs were initiated to educate the community in the suitable use of wetland ecosystems. materials and methods. infection rates of paragonimus in human and wild hosts was documented in the fuente clara sector. the presence of larvae and digenic adult worms was detected in mollusks (1,312), crabs (27) and mammals (4). sputum diagnosis was performed on samples from 18 volunteer individuals. the following determinants of water quality were measured in the stream ?la puerta?: total fecal/coliform, ph, conductivity and dissolved oxygen were measured. recreational workshops were conducted with children and teenagers for educational purposes. results. the percentage of infections found in hosts was as follows: snails, 0.07%; crabs, 55.5%; (wild) mammals, 25%; humans, 0%. during the workshops, children and teenagers identified the paragonimus hosts and the risk factors for acquiring the disease. the water of the stream was found to be unsuitable for consumption and recreation (the most probable number of total coliforms/100 ml was in a scale of 104). conclusions. fuente clara is the first urban locality in colombia where a focus of paragonimus was found. exposure to and consumption of crabs may constitute a risk for human infection.
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