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Search Results: 1 - 10 of 3561 matches for " Pamela Stanley "
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Effects of varying Notch1 signal strength on embryogenesis and vasculogenesis in compound mutant heterozygotes
Changhui Ge, Pamela Stanley
BMC Developmental Biology , 2010, DOI: 10.1186/1471-213x-10-36
Abstract: Mouse embryos expressing the hypomorphic Notch112f allele, in combination with the inactive Notch1lbd allele which lacks the Notch1 ligand binding domain, died at ~E11.5-12.5. Notch112f/lbd ES cells signaled less well than Notch112f/12f ES cells but more strongly than Notch1lbd/lbd ES cells. However, vascular defects in Notch112f/lbd yolk sac were severe and similar to Notch1lbd/lbd yolk sac. By contrast, vascular disorganization was milder in Notch112f/lbd compared to Notch1lbd/lbd embryos. The expression of Notch1 target genes was low in Notch112f/lbd yolk sac and embryo head, whereas Vegf and Vegfr2 transcripts were increased. The severity of the compound heterozygous Notch112f/lbd yolk sac phenotype suggested that the allelic products may functionally interact. By contrast, compound heterozygotes with Notch112f in combination with a Notch1 null allele (Notch1tm1Con) were capable of surviving to birth.Notch1 signaling in Notch112f/lbd compound heterozygous embryos is more defective than in compound heterozygotes expressing a hypomorphic Notch112f allele and a Notch1 null allele. The data suggest that the gene products Notch1lbd and Notch112f interact to reduce the activity of Notch112f.Notch transmembrane receptors are important regulators of cell fate determination in numerous cell types [1-3]. Notch receptors in Drosophila and mammals are covalently modified with O-fucose on many epidermal growth factor-like (EGF) repeats of the extracellular domain [4]. An important O-fucose site resides in epidermal growth factor-like repeat 12 (EGF12) which, together with EGF11, is required for canonical Notch ligand binding to Drosophila Notch [5-7] and to mammalian Notch1 [8]. A point mutation that precludes the addition of fucose to EGF12 in Drosophila Notch results in enhanced binding of both Delta and Serrate Notch ligands, and a hyperactive Notch that is refractory to Fringe [9]. However, the same mutation (Notch112f) in cultured mammalian cells gives markedly reduced
The EGF Repeat-Specific O-GlcNAc-Transferase Eogt Interacts with Notch Signaling and Pyrimidine Metabolism Pathways in Drosophila
Reto Müller, Andreas Jenny, Pamela Stanley
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0062835
Abstract: The O-GlcNAc transferase Eogt modifies EGF repeats in proteins that transit the secretory pathway, including Dumpy and Notch. In this paper, we show that the Notch ligands Delta and Serrate are also substrates of Eogt, that mutation of a putative UDP-GlcNAc binding DXD motif greatly reduces enzyme activity, and that Eogt and the cytoplasmic O-GlcNAc transferase Ogt have distinct substrates in Drosophila larvae. Loss of Eogt is larval lethal and disrupts Dumpy functions, but does not obviously perturb Notch signaling. To identify novel genetic interactions with eogt, we investigated dominant modification of wing blister formation caused by knock-down of eogt. Unexpectedly, heterozygosity for several members of the canonical Notch signaling pathway suppressed wing blister formation. And importantly, extensive genetic interactions with mutants in pyrimidine metabolism were identified. Removal of pyrimidine synthesis alleles suppressed wing blister formation, while removal of uracil catabolism alleles was synthetic lethal with eogt knock-down. Therefore, Eogt may regulate protein functions by O-GlcNAc modification of their EGF repeats, and cellular metabolism by affecting pyrimidine synthesis and catabolism. We propose that eogt knock-down in the wing leads to metabolic and signaling perturbations that increase cytosolic uracil levels, thereby causing wing blister formation.
In vivo consequences of deleting EGF repeats 8–12 including the ligand binding domain of mouse Notch1
Changhui Ge, Tongyi Liu, Xinghua Hou, Pamela Stanley
BMC Developmental Biology , 2008, DOI: 10.1186/1471-213x-8-48
Abstract: Notch1lbd/lbd embryos died at mid-gestation with a phenotype indistinguishable from Notch1 null mutants. In embryonic stem (ES) cells, Notch1lbd was expressed on the cell surface at levels equivalent to wild type Notch1, but Delta1 binding was reduced to the same level as in Notch1 null cells. In an ES cell co-culture assay, Notch signaling induced by Jagged1 or Delta1 was reduced to a similar level in Notch1lbdand Notch1 null cells. However, the Notch1lbd/lbd allele was expressed similarly to wild type Notch1 in Notch1lbd/lbd ES cells and embryos at E8.75, indicating that Notch1 signaling is not essential for the Notch1 gene to be expressed. In addition, maternal and zygotic Notch1 mutant blastocysts developed through gastrulation.Mouse Notch1 lacking the ligand binding domain is expressed at the cell surface but does not signal in response to the canonical Notch ligands Delta1 and Jagged1. Homozygous Notch1lbd/lbd mutant embryos die at ~E10 similar to Notch1 null embryos. While Notch1 is expressed in oocytes and blastocysts, Notch1 signaling via canonical ligands is dispensable during oogenesis, blastogenesis, implantation and gastrulation.Notch1 is a heterodimeric, type I transmembrane receptor that is required for cell fate decisions throughout the metazoa [1,2]. The Notch1 extracellular domain contains 36 tandem epidermal growth factor-like (EGF) repeats, and three Lin/Notch repeats. Of the 36 EGF repeats in Drosophila Notch, deletion of only EGF repeats 11 and 12 prohibits the binding of the Notch ligands Delta and Serrate in in vitro binding assays [3,4]. Notch signaling in mammals is also initiated by binding to canonical Notch ligands (Delta and Jagged) on adjacent cells. Ligand binding activates Notch signaling through two proteolytic cleavage events, first in the extracellular domain by the ADAM10 metalloprotease [5], and subsequently in the transmembrane domain by a presenilin complex with γ-secretase activity [6,7]. The released Notch intracellular doma
A new portable vibrator for plaster pouring: effect on the marginal fit at cylinder-abutment
Andrade, Pamela Candida Aires Ribas de;Luthi, Leonardo Flores;Stanley, Kyle;Cardoso, Ant?nio Carlos;
Journal of Applied Oral Science , 2012, DOI: 10.1590/S1678-77572012000500004
Abstract: objective: the aim of this study was to test a new portable vibrator for plaster pouring (developed for this purpose), comparing the effect of its use on the accuracy of working cast of implant-supported restorations to the conventional vibrator. material and methods: from a master cast with 2 implants, 30 transfer moldings were made randomly and divided into three groups: group i (gi): pouring performed in an outsourced dental laboratory with conventional plaster vibrator (10 casts), group ii (gii): pouring performed in the laboratory of the federal university of santa catarina (ufsc) with conventional plaster vibrator (10 casts) and group iii (giii): pouring performed with the portable vibrator fabricated for this study (10 casts). the position of the analogue and marginal adaptation of the infrastructure were verified by testing the single screw on the master model and on the working model. the measurement of misfit was blindly performed with a precision microscope and analyzing unit, quadra-check 200. the data were statistically analyzed by analysis of variance (anova) and the holm-sidak test (α=0.05). results: means±standard deviations were as follows: gi: 19.19±4.73 μm; gii: 21.72±5.41 μm; giii: 13.5±2.39 μm (p<0.05), with giii significantly lower as compared to the other groups. conclusion: within the limitations of this study, it was concluded that a greater accuracy of working cast was achieved when a portable vibrator was used for casting molds.
Left Atrial Volume Index in Patients with Dilated Cardiomyopathy—Correlation with Left Ventricular Function  [PDF]
Stanley George
World Journal of Cardiovascular Diseases (WJCD) , 2016, DOI: 10.4236/wjcd.2016.69035
Abstract: Background: The aim of this study was to determine the relation of left atrial (LA) volume and LA volume index with left ventricular function and to determine the association of duration of symptoms and left atrial volume index in patients with dilated cardiomyopathy. Materials and Methods: This was an observational, single centre study conducted in India. A total of 50 patients who were admitted to department of cardiology from July, 2008 to February, 2009 with diagnosis of dilated cardiomyopathy and an ejection fraction of <40% were included. Results: Of the 50 patients, 34 (68%) were males. 27 (54%) patients were in NYHA class II and 23 (46%) patients were in NYHA class III. LA volume was found to be ≥40 ml in all patients. LV function and LA volume were found to be correlated (r = -0.789, p < 0.01). Similarly, there was a correlation between LV function and LA volume index (r = -0.826, p < 0.01). There was no correlation between LA volume index and duration of symptoms (r = 0.04). Conclusion: It can be concluded that there is a strong inverse correlation between LA volume and left ventricular function and also between LA volume index and left ventricular function. The patients with NYHA class III were having larger left atrial volume than those with NYHA class II. Moreover, the duration of symptoms has no correlation with left atrial volume index.
Angiographic Profile and Treadmill Test Relationship of Women with Chest Pain Suggestive of Coronary Artery Disease  [PDF]
Stanley George
World Journal of Cardiovascular Diseases (WJCD) , 2017, DOI: 10.4236/wjcd.2017.78021
Abstract: Aim: The aim of present study was to analyze the prevalence and pattern of coronary artery disease (CAD) among women who underwent coronary angiography (CAG) and Treadmill Test (TMT). Methods: A total of 100 consecutive women patients for coronary angiogram were studied at Department of Cardiology, Government Medical College, Kottayam, Kerala, India on over a period of 6 months from February 2008. Total 65 women completed TMT stress test. The patients were subjected to CAG and TMT. Data were analyzed by using SPSS 20.0 software. Results: A total of 100 women patients were included. Among that 66% patients had hypertension and 47% patients had diabetes mellitus. The CAG revealed the presence of 17%, 13% and 15% of single vessel disease, double vessel disease, and triple vessel disease respectively. Vessel involvements were notably higher in positive TMT values during stress test. The sensitivity and specificity of TMT for detecting coronary artery disease in women were 61% and 69% respectively. Conclusion: It is concluded that, the findings of the present study effectively demonstrated higher predictive value of TMT in women with chest pain suggestive of CAD.
Respiratory rehabilitation with abdominal weights: a prospective case study  [PDF]
Stanley John Winser, Priya Stanley, George Tarion
Health (Health) , 2010, DOI: 10.4236/health.2010.25061
Abstract: Objective: Abdominal weights was used to strengthen the diaphragm of a C6 ASIA (A) tetraplegic subject with the aim of studying the long term effect of the technique as a part of respiratory rehabilitation. Setting: Department of Physical Medicine and Rehabilitation, Christian Medical College, Vellore, Tamil Nadu, India. Study Design: Prospective case study. Material and methods: The peak EMG amplitude of the diaphragm (DIA), intercostals (INT) and sternoclidomastiod (SCM) were assessed using a surface EMG and inspired lung volume (ILV) was assessed using an adjustable portable spirometer. The measurements were repeated after 3, 6, 9 and 12 months of inspiratory muscle training for a period of 15 minutes daily, 6 days a week for 12 months. Results and discussion: Peak amplitudes recorded by the EMG of DIA and SCM muscles showed a progressive increase, INT muscle did not show a consistent change. INV showed a gradual rise from 1772ml to 2760 ml over the study period. These values have the following significance: 1) Use of abdominal weights as a part of respiratory rehabilitation has beneficial long term effects; 2) In patients with tetraplegia, respiratory muscles in particular the diaphragm, are trainable in terms of muscle efficiency; 3) The improvement in the muscle efficiency obtained during the early rehabilitation can be maintained or improved using simple non sophisticated exercises like abdominal weights post discharge. Conclusions: Abdominal weights can be used as an effective adjunct to pulmonary rehabilitation in improving the efficiency of diaphragm on a long term basis, thereby reducing the risks associated with pulmonary complications.
Sub-Optimal Generation Portfolio Variance with Rate of Return Regulation  [PDF]
Stanley Keith Berry
Technology and Investment (TI) , 2010, DOI: 10.4236/ti.2010.12014
Abstract: This paper demonstrates that continuation of traditional rate-of-return electric utility regulation of transmission and distribution assets will impede the ability of customers to optimize their generation portfolios. Under linear price regulation, with increasing (decreasing) returns to scale customers will choose a more (less) risky generation portfolio than they would with no transmission and distribution asset rate-of-return regulation. Similar problems arise under non-linear (two-part) pricing of transmission and distribution assets. When the per-unit price is set at marginal cost, with increasing (decreasing) marginal cost, customers will choose a more (less) risky generation portfolio than they would with no transmission and distribution asset regulation. With price caps the optimal generation portfolio is chosen.
Where Science Meets Art: Sociology and Social Work  [PDF]
Stephanie Kelly, Tony Stanley
Sociology Mind (SM) , 2012, DOI: 10.4236/sm.2012.24044
Abstract: The nexus of neo-liberalist influences in our current risk society has produced a crisis for both New Zealand sociology and Social Work, playing out in practice domains and in the academy. This paper argues that by co-habituating and co-operating, we may have a tangible way forward. One of the biggest challenges for Social Work practitioners is to come to terms with the role of theory in the practice of their discipline—a discipline that is often fast-paced, but increasingly focused on dealing with one client at a time, and often reduced to a dyad emphasis in practise: that of client and worker. One of the biggest challenges for the sociologist embarking on a career in research is to come to terms with sociology as methodological toolkit for social activism where knowledge of theory can be applied toward sustained societal change. Both offer a methodological approach to understanding the human condition in context. Both disciplines are at risk because of neo-liberalisation, and this, we argue must be avoided by a move toward each other.
Hydronephrosis and Ureteral Obstruction in Crohn’s Disease  [PDF]
Robert Jansen, Stanley Zaslau
Open Journal of Urology (OJU) , 2013, DOI: 10.4236/oju.2013.35041
Abstract:

Hydronephrosis and ureteral obstruction are rare sequelae of Crohn’s disease. Chronic obstruction can ultimately lead to dysfunction of the affected kidney, and atypical presenting symptoms create pitfalls in diagnosis. Few reviews in the literature focus on this process and are limited to isolated case reports and case reviews. We performed a PubMed search using such terms as “Hydronephrosis” AND “Crohn’s disease” AND/OR “ureteral obstruction.” References from selected papers were reviewed for relevance and used for information-gathering as well. Ureteral obstruction most commonly occurs on the right side, due to ileal involvement. Clinical diagnosis is difficult, as symptoms are notably not genitourinary in origin; rather they are more musculoskeletal in nature. Treatment centers on disease control and temporary drainage of the affected kidney. Though rare, hydronephrosis and ureteral obstruction may develop as a result of inflammatory bowel disease. Due to atypical presenting symptoms, a high clinical suspicion is needed to affirm the diagnosis and ensure proper treatment.

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