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Search Results: 1 - 10 of 405819 matches for " Oliver Müller "
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Fatigability Assessment Using the Fatigue Index Kliniken Schmieder (FKS) Is Not Compromised by Depression  [PDF]
Christian Dettmers, Markus Riegger, Oliver Müller, Manfred Vieten
Health (Health) , 2016, DOI: 10.4236/health.2016.814147
Abstract: Background: Attractor-based kinematic gait analysis using the Fatigue Index Kliniken Schmieder (FKS), have been suggested as a sensitive tool to determine motor fatigability in persons with Multiple Sclerosis (pwMS). Hypothesis: Depression does not affect the FKS to a similar degree as in pwMS. Methods: We recruited 32 patients with major depression. Data were collected with two accelerometers attached to both ankles. Data were recorded for one minute at the beginning and at the end of the treadmill test. Attractor attributes were used to analyze the data. Results: The mean Fatigue Index Kliniken Schmieder (FKS) was 2.1. The change of the attractor (δM) was 3.7 and the change of variability (δD) was 0.6. Mean values were clearly below values of pwMS with fatigability from a previous publication. However, the individual level values from six individuals—five of them showed pain related comorbidity besides depression—fell within the lower pathological range. There was no correlation between Hamilton depression scale and the attractor attributes (δM and δD). Discussion: Depression does not affect gait as motor fatigability does in pwMS. Results from subjects with pain during the treadmill test show a moderately increased variability and moderately altered attractors.
Spin correlations in nonperturbative electron-positron pair creation by petawatt laser pulses colliding with a TeV proton beam
Tim-Oliver Müller,Carsten Müller
Physics , 2010, DOI: 10.1016/j.physletb.2010.12.023
Abstract: The influence of the electron spin degree of freedom on nonperturbative electron-positron pair production by high-energy proton impact on an intense laser field of circular polarization is analyzed. Predictions from the Dirac and Klein-Gordon theories are compared and a spin-resolved calculation is performed. We show that the various spin configurations possess very different production probabilities and discuss the transfer of helicity in this highly nonlinear process. Our predictions could be tested by combining the few-TeV proton beam at CERN-LHC with an intense laser pulse from a table-top petawatt laser source.
ATOM - an OMERO add-on for automated import of image data
Oliver Müller, Peter Lipp, Lars Kaestner
BMC Research Notes , 2011, DOI: 10.1186/1756-0500-4-382
Abstract: We have developed ATOM, a Java-based and thus platform-independent add-on for OMERO enabling automated transfer of image data from a wide variety of acquisition software packages into OMERO. ATOM provides a graphical user interface and allows pre-organisation of experimental data for the transfer.ATOM is a convenient extension of the OMERO software system. An automated interface to OMERO will be a useful tool for scientists working with file formats supported by the Bio-Formats file format library, a platform-independent library for reading the most common file formats of microscope images.The development of video enhanced microscope systems in the 1980s [1] has enabled digital microscope images in life sciences. Images were stored as a sequence of bits, analysed and organised with the aid of computer software. Initially, microscopes were predominantly operated manually, allowing a relatively low data throughput. With the emergence of high-content screening in the late 1990s [2] and thus the application of automated (i.e., software-controlled) microscopy platforms, image acquisition was accelerated [3]. As a consequence the number of stored digital images increased massively. These data had to be stored and organised following an imaging experiment. Moreover, an increasing variety of different image file formats emerged. To keep pace with this development, the imaging community requested a tool to handle arbitrary image file formats. In 2004, the Open Microscopy Environment (OME) consortium (University of Dundee, Scotland) developed the open-source software OME-Remote Objects (OMERO) and developed it further ever since [4,5]. OMERO allows users to visualise, manage and annotate digital microscope images and their corresponding metadata. Additionally, OMERO enables researchers and collaboration partners to share their digital image data over the intra- and internet. It provides the Java-based client OMERO.importer that can be used to read various image file formats a
A New Fluorescence-Based Reporter Gene Vector as a Tool for Analyzing and Fishing Cells with Activated Wnt Signaling Pathway
Johanna Apfel,Patricia Reischmann,Oliver Müller
ISRN Oncology , 2013, DOI: 10.1155/2013/603129
Abstract: The dysregulated Wnt pathway is a major cause for the activation of cell proliferation and reduced differentiation in tumor cells. Therefore the Wnt signaling pathway is the on-top target in searching for new anticancer drugs or therapeutic strategies. Although the key players of the pathway are known, no specific anti-Wnt drug entered a clinical trial by now. Several screening approaches for potential compounds have been performed with a reporter gene assay using multiple T-cell factor/lymphoid enhancer factor (TCF/LEF) binding motifs as promoters which control luciferase or β-galactosidase as reporter genes. In our work, we designed a reporter gene construct which anchors the enhanced green fluorescent protein (eGFP) to the plasma membrane. HEK 293T cells, which were stably transfected with this construct, express eGFP on the outer membrane after activation with either LiCl or WNT3A protein. Thus, cells with activated Wnt pathway could be identified and fished out of a heterogeneous cell pool by the use of magnetic-labeled anti-GFP antibodies. In summary, we present a new tool to easily detect, quantify, and sort cells with activated Wnt signaling pathway in a simple, fast, and cost-effective way. 1. Introduction The dysregulated Wnt signaling pathway is linked with cancer diseases and is also one of the most mutated signaling pathways in colorectal cancer carcinomas??[1–3]. In a normal cell, the key protein β-catenin is permanently expressed, bound to the APC protein in the destruction complex, and marked for degradation by GSK3β and β-TrCP. As a consequence the level of free β-catenin is low??[4]. With activation of the canonical Wnt signaling pathway by extracellular ligands like the Wnt proteins or by mutation of one of the destruction complex proteins and due to the following misregulation β-catenin is translocated into the nucleus and activates expression of target genes, resulting, for example, in increased cell proliferation, reduced apoptosis, or decreased cell differentiation??[5, 6]. This is supposed to be one of the major steps in carcinogenesis from a normal cell to a tumor cell??[7]. Specific drugs which interrupt this misregulation might have high potential in targeted cancer therapy with low side effects especially in the aggressive chemotherapies??[8, 9]. The searching for specific Wnt pathway inhibitors as possible cancer drugs forged ahead during the last years although none of them finished clinical trials studies until now??[10]. Most assays which are used to screen for Wnt modulating drugs are based on Korinek’s TOPFLASH reporter
New dwarf galaxy candidates in the Centaurus group
Oliver Müller,Helmut Jerjen,Bruno Binggeli
Physics , 2015, DOI: 10.1051/0004-6361/201526748
Abstract: Recent studies of the distribution and kinematics of the Milky Way and Andromeda satellite galaxy systems have confirmed the existence of coplanar, corotating structures of galaxies. In addition to the 'missing satellite problem', these structures pose a major challenge to the standard $\Lambda$CDM scenario of structure formation. We complement the efforts made by the dwarf galaxy community to extend these studies to other nearby galaxy groups by systematically searching for faint, unresolved dwarf members with a low surface brightness in the Southern Centaurus group of galaxies. The aim is to determine whether these coplanar, corotating structures are a universal phenomenon. We imaged an area of 60 square degrees (0.3 Mpc$^2$) around the M83 subgroup with the wide-field Dark Energy Camera (DECam) at the CTIO 4 m Blanco telescope in $g$ and $r$ down to a limiting surface brightness of $\mu_r\approx 30$ mag arcsec$^{-2}$. Various image-filtering techniques were applied to the DECam data to enhance the visibility of extremely low-surface brightness objects. We report the discovery of 16 new dwarf galaxy candidates in the direction of the M83 subgroup, roughly doubling the number of known dwarfs in that region. The photometric properties of the candidates, when compared to those of the Local Group, suggest membership in the M83 subgroup. The faintest objects have a central star density of $\approx1.3 L_\odot$ pc$^{-2}$ and a total magnitude of $g = 20.25$, corresponding to $M_g = -9.55$ at the nominal distance of 4.9 Mpc. The sky distribution of the new objects is significantly prolonged toward Cen A, suggesting that many of them belong to the Cen A subgroup or a common halo. We also provide updated surface photometry for the brighter, known dwarf members in the surveyed area.
The Wnt Pathway Target Gene CCND1 Changes Mitochondrial Localization and Decreases Mitochondrial Activity in Colorectal Cancer Cell Line SW480  [PDF]
Annica Vlad-Fiegen, Natalie Veronika Freytag, Susanne Dorn, Oliver Müller, Sonja Eberth
Journal of Biosciences and Medicines (JBM) , 2016, DOI: 10.4236/jbm.2016.412017
Abstract: Mutations leading to constitutive activation of the Wnt pathway and its target genes are frequently observed in cancer. The Wnt pathway promotes cell proliferation and increasing evidence supports its role also in cancer cell metabolism. This study aims to elucidate the role of the Wnt/β-catenin target gene CCND1 in these processes in colorectal cancer. We analyzed whether knock-down of CCND1 affects cell cycle progression and energy metabolism in a colorectal cancer cell line. Down-regulation of CCND1 led to retardation of the cell cycle. The proportion of cells in the G0 phase increased, while the amount of cells in the S- and G2/M phase decreased. Interestingly, knock-down of CCND1 changed the perinuclear localization of mitochondria into a homogeneous distribution within the cytosol. In addition CCND1 knock-down led to an increase of the intracellular ATP level indicating that cyclin D1 reduced mitochondrial activity. Our findings suggest that in addition to its role in cell cycle regulation, the Wnt target gene CCND1 regulates mitochondrial localization and inhibits mitochondrial activity in colorectal cancer cells.
Sensitive Commercial NASBA Assay for the Detection of Respiratory Syncytial Virus in Clinical Specimen
Ramona Liza Tillmann, Arne Simon, Andreas Müller, Oliver Schildgen
PLOS ONE , 2007, DOI: 10.1371/journal.pone.0001357
Abstract: The aim of the study was to evaluate the usability of three diagnostic procedures for the detection of respiratory syncytial virus in clinical samples. Therefore, the FDA cleared CE marked NOW? RSV ELISA, the NucliSENS? EasyQ RSV A+B NASBA, and a literature based inhouse RT-PCR protocol were compared for their relative sensitivities. Thereby, NASBA turned out to be the most sensitive method with a total number of 80 RSV positive samples out of a cohort of 251 nasopharyngeal washings from patients suffering from clinical symptoms, followed by the inhouse RT-PCR (62/251) and ELISA (52/251). Thus, NASBA may serve as a rapid and highly sensitive alternative for RSV diagnostics.
Linear Connectivity Forces Large Complete Bipartite Minors: the Patch for the Large Tree-Width Case
Jan-Oliver Fr?hlich,Theodor Müller
Mathematics , 2009,
Abstract: The recent paper "Linear Connectivity Forces Large Complete Bipartite Minors" by Boehme et al. relies on a structure theorem for graphs with no H-minor. The sketch provided of how to deduce this theorem from the work of Robertson and Seymour appears to be incomplete. To fill this gap, we modify the main proof of that paper to work with a mere restatement of Robertson and Seymour's original results instead.
Stochastic differential equations for models of non-relativistic matter interacting with quantized radiation fields
Batu Güneysu,Oliver Matte,Jacob Schach Mller
Mathematics , 2014,
Abstract: We derive and discuss stochastic differential equations associated with the heat semi-groups of the standard model of non-relativistic quantum electrodynamics and of corresponding fiber Hamiltonians for translation invariant systems. In particular, we re-derive Feynman-Kac type formulas for the semi-groups in the scalar case and obtain new Feynman-Kac formulas involving series of time-ordered integrals of certain annihilation-preservation-creation processes for matrix-valued Hamiltonians. Furthermore, we derive stochastic differential equations and Feynman-Kac representations for an operator-valued integral kernel of the semi-group. As a byproduct we obtain analogous results for Nelson's model.
Wound Healing in Mice with High-Fat Diet- or ob Gene-Induced Diabetes-Obesity Syndromes: A Comparative Study
Oliver Seitz,Christoph Schürmann,Nadine Hermes,Elke Müller,Josef Pfeilschifter,Stefan Frank,Itamar Goren
Experimental Diabetes Research , 2010, DOI: 10.1155/2010/476969
Abstract: In the past, the genetically diabetic-obese diabetes/diabetes (db/db) and obese/obese (ob/ob) mouse strains were used to investigate mechanisms of diabetes-impaired wound healing. Here we determined patterns of skin repair in genetically normal C57Bl/6J mice that were fed using a high fat diet (HFD) to induce a diabetes-obesity syndrome. Wound closure was markedly delayed in HFD-fed mice compared to mice which had received a standard chow diet (CD). Impaired wound tissue of HFD mice showed a marked prolongation of wound inflammation. Expression of vascular endothelial growth factor (VEGF) was delayed and associated with the disturbed formation of wound margin epithelia and an impaired angiogenesis in the reduced granulation tissue. Normal wound contraction was retarded and disordered. Wound disorders in obese C57Bl/6J mice were paralleled by a prominent degradation of the inhibitor of NFκB (IκB-α) in the absence of an Akt activation. By contrast to impaired wound conditions in ob/ob mice, late wounds of HFD mice did not develop a chronic inflammatory state and were epithelialized after 11 days of repair. Thus, only genetically obese and diabetic ob/ob mice finally developed chronic wounds and therefore represent a better suited experimental model to investigate diabetes-induced wound healing disorders.
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