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Search Results: 1 - 10 of 111982 matches for " Olesya O. Panasenko "
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The CCR4-NOT Complex Physically and Functionally Interacts with TRAMP and the Nuclear Exosome
Nowel Azzouz, Olesya O. Panasenko, Geoffroy Colau, Martine A. Collart
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0006760
Abstract: Background Ccr4-Not is a highly conserved multi-protein complex consisting in yeast of 9 subunits, including Not5 and the major yeast deadenylase Ccr4. It has been connected functionally in the nucleus to transcription by RNA polymerase II and in the cytoplasm to mRNA degradation. However, there has been no evidence so far that this complex is important for RNA degradation in the nucleus. Methodology/Principal Findings In this work we point to a new role for the Ccr4-Not complex in nuclear RNA metabolism. We determine the importance of the Ccr4-Not complex for the levels of non-coding nuclear RNAs, such as mis-processed and polyadenylated snoRNAs, whose turnover depends upon the nuclear exosome and TRAMP. Consistently, mutation of both the Ccr4-Not complex and the nuclear exosome results in synthetic slow growth phenotypes. We demonstrate physical interactions between the Ccr4-Not complex and the exosome. First, Not5 co-purifies with the exosome. Second, several exosome subunits co-purify with the Ccr4-Not complex. Third, the Ccr4-Not complex is important for the integrity of large exosome-containing complexes. Finally, we reveal a connection between the Ccr4-Not complex and TRAMP through the association of the Mtr4 helicase with the Ccr4-Not complex and the importance of specific subunits of Ccr4-Not for the association of Mtr4 with the nuclear exosome subunit Rrp6. Conclusions/Significance We propose a model in which the Ccr4-Not complex may provide a platform contributing to dynamic interactions between the nuclear exosome and its co-factor TRAMP. Our findings connect for the first time the different players involved in nuclear and cytoplasmic RNA degradation.
The Not4 E3 Ligase and CCR4 Deadenylase Play Distinct Roles in Protein Quality Control
David Halter, Martine A. Collart, Olesya O. Panasenko
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0086218
Abstract: Eukaryotic cells control their proteome by regulating protein production and protein clearance. Protein production is determined to a large extent by mRNA levels, whereas protein degradation depends mostly upon the proteasome. Dysfunction of the proteasome leads to the accumulation of non-functional proteins that can aggregate, be toxic for the cell, and, in extreme cases, lead to cell death. mRNA levels are controlled by their rates of synthesis and degradation. Recent evidence indicates that these rates have oppositely co-evolved to ensure appropriate mRNA levels. This opposite co-evolution has been correlated with the mutations in the Ccr4-Not complex. Consistently, the deadenylation enzymes responsible for the rate-limiting step in eukaryotic mRNA degradation, Caf1 and Ccr4, are subunits of the Ccr4-Not complex. Another subunit of this complex is a RING E3 ligase, Not4. It is essential for cellular protein solubility and has been proposed to be involved in co-translational quality control. An open question has been whether this role of Not4 resides strictly in the regulation of the deadenylation module of the Ccr4-Not complex. However, Not4 is important for proper assembly of the proteasome, and the Ccr4-Not complex may have multiple functional modules that participate in protein quality control in different ways. In this work we studied how the functions of the Caf1/Ccr4 and Not4 modules are connected. We concluded that Not4 plays a role in protein quality control independently of the Ccr4 deadenylase, and that it is involved in clearance of aberrant proteins at least in part via the proteasome.
The Not5 Subunit of the Ccr4-Not Complex Connects Transcription and Translation
Zoltan Villanyi,Virginie Ribaud,Sari Kassem,Olesya O. Panasenko,Zoltan Pahi,Ishaan Gupta,Lars Steinmetz,Imre Boros,Martine A. Collart
PLOS Genetics , 2014, DOI: doi/10.1371/journal.pgen.1004569
Abstract: Recent studies have suggested that a sub-complex of RNA polymerase II composed of Rpb4 and Rpb7 couples the nuclear and cytoplasmic stages of gene expression by associating with newly made mRNAs in the nucleus, and contributing to their translation and degradation in the cytoplasm. Here we show by yeast two hybrid and co-immunoprecipitation experiments, followed by ribosome fractionation and fluorescent microscopy, that a subunit of the Ccr4-Not complex, Not5, is essential in the nucleus for the cytoplasmic functions of Rpb4. Not5 interacts with Rpb4; it is required for the presence of Rpb4 in polysomes, for interaction of Rpb4 with the translation initiation factor eIF3 and for association of Rpb4 with mRNAs. We find that Rpb7 presence in the cytoplasm and polysomes is much less significant than that of Rpb4, and that it does not depend upon Not5. Hence Not5-dependence unlinks the cytoplasmic functions of Rpb4 and Rpb7. We additionally determine with RNA immunoprecipitation and native gel analysis that Not5 is needed in the cytoplasm for the co-translational assembly of RNA polymerase II. This stems from the importance of Not5 for the association of the R2TP Hsp90 co-chaperone with polysomes translating RPB1 mRNA to protect newly synthesized Rpb1 from aggregation. Hence taken together our results show that Not5 interconnects translation and transcription.
Principles of the analysis of structure of chaffinch song (Fringilla coelebs L.). Individual variability of song  [PDF]
Olesya Astakhova, Irina Byome
Open Journal of Animal Sciences (OJAS) , 2012, DOI: 10.4236/ojas.2012.23026
Abstract: Song of birds is subtle and flexible species specific feature, like as the morphology signs. Species specific song of chaffinch (Fringilla coelebs L.) can be judged as precise character in the image on sonograms. The song has the certain structure and shares (divides) on the parts, elements, which consists of them. Individual variability of songs of one type will consist in unique singing each of chaffinch males, who form a local population.
Chaffinch (Fringilla coelebs L.) song in populations of the East Europe  [PDF]
Olesya Anatolyevna Astakhova
Open Journal of Animal Sciences (OJAS) , 2012, DOI: 10.4236/ojas.2012.24029
Abstract: At studying of song organizations of chaffinch (Fringilla coelebs L.) are found out unique parities of song types in different local populations of a species-specific area. Thus in different areas of the East Europe are forming original (different) song cultures of chaffinch—the certain set of song types including so-called “dialect song forms”. The complex interrelation of geographical variability of chaffinch song in many respects gives a support at an evolutionary view in the given aspect.
Vocal Variability of Chaffinch Song (Fringilla coelebs L.) as a Condition of Cultural Evolution in Local Populations  [PDF]
Olesya Anatolyevna Astakhova
Open Journal of Animal Sciences (OJAS) , 2014, DOI: 10.4236/ojas.2014.42009
Abstract: There are such characteristics of a matter of the nature, as variability and stability (tradition, norm). Probably, these opposites process as if other qualities of different forms of energy also create “movement” development. Thus the given properties of a matter of the nature can be considered at different levels of its organization. The singing of many passerine birds is incontrovertible feature of their life which determines and builds a reproductive cycle. By studying song repertoire of many sparrow species in details, it was revealed that the individual has not only one, but some types of songs. Thus spring singing represents the multifunctional phenomenon and can bear (carry) various values. The singing is not only a means of attracting females, but also a means of intimidation of the contender and delimitation of nested territory. Variants or types of species-specific song are individually various and distributed between individuals of a population. The complex interrelation of geographical variability of chaffinch song in many respects gives a support at an evolutionary view in the given aspect.
Degree of prevalence of different song types of chaffinch (Fringilla coelebs L.) in populations of the European Russia  [PDF]
Olesya Anatolyevna Astakhova
Open Journal of Animal Sciences (OJAS) , 2014, DOI: 10.4236/ojas.2014.41005
Abstract:

When studying song organizations of chaffinch (Fringilla coelebs L.), we find the unique typological parities (ratio) of songs in different local populations of a species-specific area. The degree of prevalence of different chaffinch song types can’t correspond to percentage proceeding from their general (common) number in a population. Thus different areas of Russia are forming original (different) song cultures of chaffinch, consisting of the certain set and a parity (ratio) of song types, frequently including the so-called dialect song forms, that have been observed. The complex interrelation of geographical variability and structural variability of chaffinch song in many respects gives a support in an evolutionary view from the given aspect.

Diatoms from Middle Miocene Continental Deposits of Primorye  [PDF]
Olesya Yu. Likhacheva
Journal of Geoscience and Environment Protection (GEP) , 2014, DOI: 10.4236/gep.2014.23012
Abstract:

Diatoms from Middle Miocene deposits of Khanka Lake’s northwestern shore (Primorye) were studied using light and scanning microscopes. The analyses of diatom flora composition and species diversity showed that there was a dominace of several taxa that made possible the recognition of two diatom complexes. The lower complex differs from the upper one by higher species diversity, by presence of many subtropical diatoms and absence of pronounced dominant species. Differences between the complexes can be explained by the progressing climatic cooling and predominance of fluvial-lakustrine sedimentation over the typically lake one due to development of a wide system of river valleys.

How Safe the Human-Robot Coexistence Is? Theoretical Presentation
Olesya Ogorodnikova
Acta Polytechnica Hungarica , 2009,
Abstract: It is evident that industrial robots are able to generate forces high enough toinjure a human. To prevent this, robots have to work within a restricted space that includesthe entire region reachable by any part of the robot. However, more and more robotapplications require human intervention due to superior abilities for some tasksperformance. In this paper we introduce danger/safety indices which indicate a level of therisk during interaction with robots, which are based on a robot’s critical characteristicsand on a human’s physical and mental constrains. Collision model for a 1 DOF robot and“human” was developed. Case study with further simulations was provided for the PUMA560 robot.
Creating an Active Awareness System for Humans in Robotic Workcell
Olesya Ogorodnikova
Acta Polytechnica Hungarica , 2008,
Abstract: This article is devoted to the problem of human’s security and safety at the timeof any interaction with robots. We already discussed robot system reliability in the previouspaper [1], now we emphasize the role of attention and human awareness with respect to therobot’s performance in vicinity. We analyzed human’s cognitive and physical abilities inambient environment perception with the aim at effective warning system implementation.We introduced warning system interface on the basis of vibrotactile cuing, proposed analgorithm for robot controller and interface that impart tactile and visual information tohuman basing on data acquired from external sensory unit.
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