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OALib Journal期刊

ISSN: 2333-9721

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A New Histone Structure Which Binds DNA at Its Eight Subunit N-Termini  [PDF]
Ken Biegeleisen
Open Access Library Journal (OALib Journal) , 2016, DOI: 10.4236/oalib.1102386
Abstract: A new model for the nucleosome is presented. The histone octamer core is unchanged, but the location of the DNA is different. Since the highest number, and highest concentration of positively- charged amino acid residues is located not in the “superhelical ramp” of the octamer core, but rather in the domain of the eight histone subunit N-termini collectively, the DNA is therefore placed there. The role models for the protein and DNA structures in the N-terminal domain are taken from the comparable role models for protein and DNA in the protamine-DNA complex in sperm cells. The histone subunit N-termini are each modeled as beta-strands, with psi/phi values of approximately /﹣130.5° respectively, which gives a straight chain. The DNA is modeled according to the “straight ladder” model of Tai Te Wu. Each DNA phosphate group is bound to a lysine or arginine residue of histone by a 3 A salt bridge. The new model lends itself so readily to further models of higher-order chromatin structure that the problem shifts entirely, from one of deducing any higher-order structure at all, to one of distinguishing between several models which compete for our attention.
Synthesis of cage-like octa(trimethylsiloxy)silsesquioxane
Chunye Hu,Yan Qin,Changyou Yuan
Chinese Science Bulletin , 1999, DOI: 10.1007/BF02884918
Abstract: Cage-like octa(trimethylsiloxy)silsesquioxane [ (Me3SiO)SiO1.5]8 has been synthesized via the trimethylsilylation of cubic tetramethylammonium silicate octamer [(Me4NO)-SiO1.5]8 with chlorotrimethylsilane. The silicate octamer can be selectively formed by the reaction of tetraethoxysilane Si(OEt)4 with aqueous tetramethylammonium hydroxide in equal molar ratio. Elementary analysis, FT-IR,1H,13C,29Si NMR are used to characterize these silsesquioxanes.
NodD binds to target DNA in isologous octamer
Songtao Liu,Huafeng Lü,Guofan Hong
Science China Life Sciences , 1998, DOI: 10.1007/BF02882900
Abstract: NodD, the major regulatory protein of nodulation, was partially purified from Rhizobium leguminosarum 8401(pIJ1518), and its binding sequences within nodF promoter of R. l. bv. viciae were determined by DNase I footprinting. A series of techniques based on gel retardation were used to analyze the NodD-target DNA interaction, showing that NodD binds to target DNA in isologous octamer.
NodD binds to target DNA in isologous octamer

LIU Songtao,LV Huafeng,HONG Guofan,

中国科学C辑(英文版) , 1998,
Abstract: NodD, the major regulatory protein of nodulation, was partially purified from Rhizobium leguminosarum 8401(pIJ1518), and its binding sequences within nodF promoter of R.l. bv. viciae were determined by DNase I footprinting. A series of techniques based on gel retardation were used to analyze the NodD_target DNA interaction, showing that NodD binds to target DNA in isologous octamer.
Characteristics and neural-like differentiation of mesenchymal stem cells derived from foetal porcine bone marrow
Ying Liu,Limei Liu,Xin Ma,Yupeng Yin
Bioscience Reports , 2013, DOI: 10.1042/bsr20120023
Abstract: MSCs (mesenchymal stem cells) are a stem cell source that can be easily obtained from bone marrow. Despite the increasing importance of the pig as a large animal model, little is known about foetal pMSCs (porcine MSCs). In this study, we observed the gene expression of pluripotent markers in foetal pMSCs and the capacity of pMSCs to differentiate into adipocytes, osteocytes and neural-like cells using quantitative RT–PCR (reverse transcription–PCR), normal histological staining and immunohistochemistry. Foetal pMSCs have either a spindle or a flattened shape, and flow cytometry revealed the expression of the MSC-related proteins CD44 and CD105 (endoglin) but not CD34 and CD45. pMSCs express pluripotent markers such as Oct4 (octamer-binding transcription factor 4) and Nanog at the protein and mRNA levels. qRT-PCR (quantitative real-time PCR) analyses revealed that pMSCs expressed nestin [for NSCs (neural stem cells)]. Immunocytochemical and RT–PCR data showed that 29% and 23% of pMSCs expressed MAP2 (microtubule-associated protein 2) for neurons and β-tubulin III (Tuj1) for immature neurons, respectively, after induction of neural differentiation. These findings demonstrate the plasticity of pMSCs and their potential for use in cellular replacement therapy for neural diseases.
Induced Pluripotent Stem Cells and Their Applications
Handan SEV?M,?zer Aylin GüRPINAR
Marmara Medical Journal , 2012,
Abstract: Pluripotency is a property of a cell that allows it to develop as any of the cell types of the body. Embryonic stem cells (ESCs) are unique cells in the organism and they have a pluripotent capacity. Induced pluripotent stem cells (IPSCs) is a term that describes somatic cells having a pluripotent capacity induced by the viral transfection of special genes. This term was firstly used in 2006 by Takahashi and Yamanaka in their experimental work. c-Myc, Sox-2, Oct and Klf-4 genes are used for the transfection of somatic cells in order to obtain IPSCs. IPSC colonies are produced by using a successful transfection process. IPSCs have pluripotent stem cell specialities like growing potential in a culture system, having a DNA methylation pattern, an ability to form teratomas, to generate three germ line components and to generate chimeric organisms which pluripotent ESCs have. Concerning the ethical problems of working with the ESCs, IPSCs can be a unique source for pluripotency studies. IPSCs with their pluripotent capacity can be used for cell therapies in diseases which have irreversible cell defects. IPSCs can also be used to form autologus implants with no immune response. Therefore, IPSCs can be used for cell therapies, drug research or disease models. In this review, we give some information about obtaining IPSCs and today's research areas that have been opened by the use of these cells. (Marmara Medical Journal 2012;25:5-9)
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