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Search Results: 1 - 10 of 483082 matches for " Norma A. Martínez-Gallardo "
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Burkholderia ambifaria and B. caribensis Promote Growth and Increase Yield in Grain Amaranth (Amaranthus cruentus and A. hypochondriacus) by Improving Plant Nitrogen Uptake
Fannie I. Parra-Cota, Juan J. Pe?a-Cabriales, Sergio de los Santos-Villalobos, Norma A. Martínez-Gallardo, John P. Délano-Frier
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0088094
Abstract: Grain amaranth is an emerging crop that produces seeds having high quality protein with balanced amino-acid content. However, production is restricted by agronomic limitations that result in yields that are lower than those normally produced by cereals. In this work, the use of five different rhizobacteria were explored as a strategy to promote growth and yields in Amaranthus hypochondriacus cv. Nutrisol and A. cruentus cv. Candil, two commercially important grain amaranth cultivars. The plants were grown in a rich substrate, high in organic matter, nitrogen (N), and phosphorus (P) and under greenhouse conditions. Burkholderia ambifaria Mex-5 and B. caribensis XV proved to be the most efficient strains and significantly promoted growth in both grain amaranth species tested. Increased grain yield and harvest index occurred in combination with chemical fertilization when tested in A. cruentus. Growth-promotion and improved yields correlated with increased N content in all tissues examined. Positive effects on growth also occurred in A. cruentus plants grown in a poor soil, even after N and P fertilization. No correlation between non-structural carbohydrate levels in roots of inoculated plants and growth promotion was observed. Conversely, gene expression assays performed at 3-, 5- and 7-weeks after seed inoculation in plants inoculated with B. caribensis XV identified a tissue-specific induction of several genes involved in photosynthesis, sugar- and N- metabolism and transport. It is concluded that strains of Burkholderia effectively promote growth and increase seed yields in grain amaranth. Growth promotion was particularly noticeable in plants grown in an infertile soil but also occurred in a well fertilized rich substrate. The positive effects observed may be attributed to a bio-fertilization effect that led to increased N levels in roots and shoots. The latter effect correlated with the differential induction of several genes involved in carbon and N metabolism and transport.
Transcriptomic analysis of grain amaranth (Amaranthus hypochondriacus) using 454 pyrosequencing: comparison with A. tuberculatus, expression profiling in stems and in response to biotic and abiotic stress
John P Délano-Frier, Hamlet Avilés-Arnaut, Kena Casarrubias-Castillo, Gabriela Casique-Arroyo, Paula A Castrillón-Arbeláez, Luis Herrera-Estrella, Julio Massange-Sánchez, Norma A Martínez-Gallardo, Fannie I Parra-Cota, Erandi Vargas-Ortiz, María G Estrada-Hernández
BMC Genomics , 2011, DOI: 10.1186/1471-2164-12-363
Abstract: A total of 2,700,168 raw reads were obtained from six 454 pyrosequencing runs, which were assembled into 21,207 high quality sequences (20,408 isotigs + 799 contigs). The average sequence length was 1,064 bp and 930 bp for isotigs and contigs, respectively. Only 5,113 singletons were recovered after quality control. Contigs/isotigs were further incorporated into 15,667 isogroups. All unique sequences were queried against the nr, TAIR, UniRef100, UniRef50 and Amaranthaceae EST databases for annotation. Functional GO annotation was performed with all contigs/isotigs that produced significant hits with the TAIR database. Only 8,260 sequences were found to be homologous when the transcriptomes of A. tuberculatus and A. hypochondriacus were compared, most of which were associated with basic house-keeping processes. Digital expression analysis identified 1,971 differentially expressed genes in response to at least one of four stress treatments tested. These included several multiple-stress-inducible genes that could represent potential candidates for use in the engineering of stress-resistant plants. The transcriptomic data generated from pigmented stems shared similarity with findings reported in developing stems of Arabidopsis and black cottonwood (Populus trichocarpa).This study represents the first large-scale transcriptomic analysis of A. hypochondriacus, considered to be a highly nutritious and stress-tolerant crop. Numerous genes were found to be induced in response to (a)biotic stress, many of which could further the understanding of the mechanisms that contribute to multiple stress-resistance in plants, a trait that has potential biotechnological applications in agriculture.The genus Amaranthus L. (Caryophyllales: Amaranthaceae) comprises C4 dicotyledonous herbaceous plants classified into approximately 70 species. It has a worldwide distribution, although most species are found in the warm temperate and tropical regions of the world [1,2]. Many amaranth species
Primer registro de una colonia de Leptonycteris curasoae (Chiroptera: Phyllostomidae) en Baja California, México
Guevara-Carrizales, Aldo A.;Martínez-Gallardo, Roberto;Moreno-Valdez, Arnulfo;
Revista mexicana de biodiversidad , 2010,
Abstract: we report the first record of a colony of the phillostomid bat leptonycteris curasoae for baja california, mexico. this region represents the second locality reported for this species in the state.
Primer registro de una colonia de Leptonycteris curasoae (Chiroptera: Phyllostomidae) en Baja California, México First record of a Leptonycteris curasoae (Chiroptera: Phyllostomidae) colony in Baja California, Mexico
Aldo A. Guevara-Carrizales,Roberto Martínez-Gallardo,Arnulfo Moreno-Valdez
Revista mexicana de biodiversidad , 2010,
Abstract: En el presente trabajo se registra por primera vez una colonia del murciélago filostomido Leptonycteris curasoae para Baja California, México, que también constituye la segunda localidad en el estado donde se registra esta especie. We report the first record of a colony of the phillostomid bat Leptonycteris curasoae for Baja California, Mexico. This region represents the second locality reported for this species in the state.
Metabolic and enzymatic changes associated with carbon mobilization, utilization and replenishment triggered in grain amaranth (Amaranthus cruentus) in response to partial defoliation by mechanical injury or insect herbivory
Paula Castrillón-Arbeláez, Norma Martínez-Gallardo, Hamlet Arnaut, Axel Tiessen, John Délano-Frier
BMC Plant Biology , 2012, DOI: 10.1186/1471-2229-12-163
Abstract: Defoliation by insect herbivory (HD) or mechanical damage (MD) led to a rapid and transient reduction of non-structural carbohydrates (NSC) in all tissues examined. This correlated with a short-term induction of foliar sucrolytic activity, differential gene expression of a vacuolar invertase and its inhibitor, and induction of a sucrose transporter gene. Leaf starch in defoliated plants correlated negatively with amylolytic activity and expression of a β-amylase-1 gene and positively with a soluble starch synthase gene. Fatty-acid accumulation in roots coincided with a high expression of a phosphoenolpyruvate/phosphate transporter gene. In all tissues there was a long-term replenishment of most metabolite pools, which allowed damaged plants to maintain unaltered growth and grain yield. Promoter analysis of ADP-glucose pyrophosphorylase and vacuolar invertase genes indicated the presence of cis-regulatory elements that supported their responsiveness to defoliation. HD and MD had differential effects on transcripts, enzyme activities and metabolites. However, the correlation between transcript abundance and enzymatic activities was very limited. A better correlation was found between enzymes, metabolite levels and growth and reproductive parameters.It is concluded that a rapid reduction of NSC reserves in leaves, stems and roots followed by their long-term recovery underlies tolerance to defoliation in grain amaranth. This requires the coordinate action of genes/enzymes that are differentially affected by the way leaf damage is performed. Defoliation tolerance in grain is a complex process that can’t be fully explained at the transcriptomic level only.The genus Amaranthus is comprised by more than 60 species. It belongs to the Amaranthaceae family that also includes sugar beet, spinach, Chenopodium spp. and several halophytes [1]. They are C4 dicotyledonous annual plants, many of which are ubiquitous weeds (e.g. A. spinosus, A. tuberculatus and A. retroflexus), wherea
Betacyanin Biosynthetic Genes and Enzymes Are Differentially Induced by (a)biotic Stress in Amaranthus hypochondriacus
Gabriela Casique-Arroyo, Norma Martínez-Gallardo, Luis González de la Vara, John P. Délano-Frier
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0099012
Abstract: An analysis of key genes and enzymes of the betacyanin biosynthetic pathway in Amaranthus hypochondriacus (Ah) was performed. Complete cDNA sequence of Ah genes coding for cyclo-DOPA 5-O glucosyltransferase (AhcDOPA5-GT), two 4, 5-DOPA-extradiol-dioxygenase isoforms (AhDODA-1 and AhDODA-2, respectively), and a betanidin 5-O-glucosyltransferase (AhB5-GT), plus the partial sequence of an orthologue of the cytochrome P-450 R gene (CYP76AD1) were obtained. With the exception AhDODA-2, which had a closer phylogenetic relationship to DODA-like genes in anthocyanin-synthesizing plants, all genes analyzed closely resembled those reported in related Caryophyllales species. The measurement of basal gene expression levels, in addition to the DOPA oxidase tyrosinase (DOT) activity, in different tissues of three Ah genotypes having contrasting pigmentation levels (green to red-purple) was determined. Additional analyses were performed in Ah plants subjected to salt and drought stress and to two different insect herbivory regimes. Basal pigmentation accumulation in leaves, stems and roots of betacyanic plants correlated with higher expression levels of AhDODA-1 and AhB5-GT, whereas DOT activity levels coincided with pigment accumulation in stems and roots and with the acyanic nature of green plants, respectively, but not with pigmentation in leaves. Although the abiotic stress treatments tested produced changes in pigment levels in different tissues, pigment accumulation was the highest in leaves and stems of drought stressed betacyanic plants, respectively. However, tissue pigment accumulation in stressed Ah plants did not always correlate with betacyanin biosynthetic gene expression levels and/or DOT activity. This effect was tissue- and genotype-dependent, and further suggested that other unexamined factors were influencing pigment content in stressed Ah. The results obtained from the insect herbivory assays, particularly in acyanic plants, also support the proposal that these genes could have functions other than betacyanin biosynthesis.
Insuficiencia cardíaca en la enfermedad renal crónica avanzada: relación con el acceso vascular
Martínez-Gallardo,Rocío; Ferreira-Morong,Flavio; García-Pino,Guadalupe; Cerezo-Arias,Isis; Hernández-Gallego,Román; Caravaca,Francisco;
Nefrología (Madrid) , 2012,
Abstract: introduction: congestive heart failure (chf) is a common complication in patients with chronic kidney disease (ckd). in addition to classical risk factors (e.g. age and pre-existing cardiac diseases), other potential reversible abnormalities linked to ckd such as anaemia, volume overload, or vascular access placement may also influence the incidence and severity of acute exacerbations of chf. objective: this study aims to determine the incidence and main determinants of chf in a cohort of patients with stage 4-5 pre-dialysis ckd. patients and method: the study group consisted of 562 patients (mean age: 65±15 years, 260 females, 31% diabetics). native arteriovenous fistulas (avf) were created in 160 patients who chose haemodialysis as the initial technique for renal replacement therapy. the main outcome variables were: acute decompensated chf (defined by standard criteria), dialysis initiation (planned and unplanned), and death before dialysis initiation. in addition to demographics, comorbidities, and clinical and biochemical data, avf creation was also included as a potential determinant of chf in multiple logistic regression models. results: ninety-five patients (17%) developed at least one episode of acute decompensated chf, and the incidence rate was 19 episodes per 1000 patient-years. in addition to classical risk factors (age, female sex, obesity, diabetes, and previous history of chf or coronary artery disease), creation of a successful avf significantly increased the risk of chf (or=9.54, 95% ci: 4.84-18.81, p<.0001). in 47 out of 95 patients who developed chf, a functioning avf had previously been created, 92% of which were upper arm native avf, with a median of 51 days between the surgical procedure and chf episode. the mortality of patients with chf was similar to that of the rest of the study patients, although unplanned dialysis initiation was significantly more frequent in those who developed chf. conclusions: acute decompensated chf episodes are commo
Primer registro de Eumops perotis (Chiroptera: Molossidae) en la Peninísula de Baja California
Martínez-Gallardo, R;Guevara-Carrizales, AA;Couoh-de la Garza, R;Flores-Rojas, Ε;
Universidad y ciencia , 2008,
Abstract: one specimen of eumpos perotis was collected at sierra de juarez, baja california, mexico. this record represents the first one of this species for the state and for the baja california peninsula.
Intoxicación por paraquat
Norma A. Hernández Hernández,Miguel A. Martínez
Salud en Tabasco , 2000,
Abstract: En el periodo comprendido de 1996 a 1999, se revisaron los expedientes clínicos de enfermos intoxicados por biperidilos (Paraquat), para determinar las principales manifestaciones, complicaciones, tratamiento y pronóstico del tóxico. Se realizó un estudio retrospectivo, transversal, descriptivo y observacional, en el área de Medicina Interna del Hospital General Dr. Gustavo A. Rovirosa Pérez. Se estudiaron 18 pacientes con intoxicación por paraquat, dos se excluyeron para fines de evolución pero no de mortalidad por datos incompletos en el expediente. De los l6 pacientes estudiados con intoxicación por biperidilos (Paraquat), el sexo masculino ocupó el mayor número, l3 hombres (81.25%) y 3 mujeres, con un rango de edad de 15 a 74 a os. El producto químico comercial más utilizado fue el Gramoxone, siendo la principal vía de intoxicación la oral, en 93.75% con cantidades variables entre 3 y 750cc. Las principales manifestaciones al ingreso se incluyeron en la fase 1, que consiste en inflamación, edema y ulceración de la capa mucosa de tubo digestivo, y fase 2 con da o hepático y renal. Las principales complicaciones fueron a nivel digestivo, renal, hepático y pulmonar. El tratamiento incluyó medidas básicas de urgencias, generales y, en algunos casos especiales diálisis peritoneal aguda. Conclusiones. Los factores predisponentes fueron intento suicida por depresión reactiva breve y alcoholismo. La complicación pulmonar fue determinante en la mortalidad, debido a que el tejido pulmonar adquiere más concentración del tóxico que otros tejidos. A pesar de su alta toxicidad, la mortalidad fue del 56%. El tratamiento debe ser vigoroso e iniciado tan rápido como sea posible.
Industria petroquímica y cambios socioeconómicos regionales en la costa del Golfo de México. El caso del sureste de Veracruz
María Teresa Sánchez Salazar,Norma Martínez Laguna,Maribel Martínez Galicia
Investigaciones geográficas , 1999,
Abstract: La industria petroquímica ha desempe ado un papel fundamental en la estructuración y organización de algunos espacios costeros del país, entre ellos el de la región sureste de Veracruz. El impulso que recibió esta región en el contexto del auge petrolero desencadeno una serie de procesos de cambio sociodemográficos, económicos y urbano-regionales que, por su dinámica acelerada, rebasaron la capacidad de los gobiernos locales, estatal y federal para dirigirlos en el marco de una política de ordenamiento territorial, lo que trajo como resultado profundos desequilibrios socioeconómicos en el interior de la región.
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