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Search Results: 1 - 10 of 1807 matches for " Noboru Hayashi "
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Effects of Aerobic Exercise on the Intramuscular Lipid and Glycogen Content of Fiber Types in Soleus Muscles of Non-Alcoholic Steatohepatitis Model Rats  [PDF]
Miyako Mochizuki, Emi Hayashi, Atsushi Yoshimura, Yuko Toyoda, Lin Mei, Noboru Hasegawa
Open Journal of Endocrine and Metabolic Diseases (OJEMD) , 2015, DOI: 10.4236/ojemd.2015.510017
Abstract: We studied the effects of exercise on muscle mitochondria, and lipid and glycogen content in non-alcoholic steatohepatitis (NASH) model rats. Male Sprague-Dawley rats were randomly separated into 3 groups: the control group was fed standard chow; the NASH group was fed a methionine-choline-deficient high-fat diet (MCD); the NASH-exercise group was fed the MCD and exercised three times a week. Exercise training consisted of continuous running for thirty minutes at a 13 m/min, 6° slope on a motor-driven rodent treadmill for 6 weeks. Mitochondria content in NASH group decreased in the both fiber types compared with those of the control group. As compared between the NASH and NASH-exercise groups, however, exercise not only promoted significant improvements in liver fibrosis and cirrhosis and triglyceride (TG) content but also increased mitochondria content in type I muscle fiber in particular. These data suggest that exercise improved hepatic steatosis in NASH model rats and can prevent the progression of NASH.
Bi-Directional Signal Transmission in EPR Correlation  [PDF]
Noboru Hokkyo
Journal of Modern Physics (JMP) , 2014, DOI: 10.4236/jmp.2014.514134

A solution of nonlocal EPR correlation between counter-propagating pair of polarization entangled photons emitted from a common source at S and detected at points P and Q is sought outside the EPR’s reality criterion of local causality but within the framework of time-symmetric quantum electrodynamics allowing the bi-directional signal transmission P \"\" S \"\" Q on the double-light cone where the future and the past cones share common light paths connecting the photon source S and the detection points P and Q.

Resurrection of a Bull by Cloning from Organs Frozen without Cryoprotectant in a ?80°C Freezer for a Decade
Yoichiro Hoshino, Noboru Hayashi, Shunji Taniguchi, Naohiko Kobayashi, Kenji Sakai, Tsuyoshi Otani, Akira Iritani, Kazuhiro Saeki
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0004142
Abstract: Frozen animal tissues without cryoprotectant have been thought to be inappropriate for use as a nuclear donor for somatic cell nuclear transfer (SCNT). We report the cloning of a bull using cells retrieved from testicles that had been taken from a dead animal and frozen without cryoprotectant in a ?80°C freezer for 10 years. We obtained live cells from defrosted pieces of the spermatic cords of frozen testicles. The cells proliferated actively in culture and were apparently normal. We transferred 16 SCNT embryos from these cells into 16 synchronized recipient animals. We obtained five pregnancies and four cloned calves developed to term. Our results indicate that complete genome sets are maintained in mammalian organs even after long-term frozen-storage without cryoprotectant, and that live clones can be produced from the recovered cells.
Quantitation of Pyrrole-Imidazole Polyamide in Rat Plasma by High-Performance Liquid Chromatography Coupled with UV Detection
Tomonori Kamei,Takahiko Aoyama,Chihiro Tanaka,Takafumi Nagashima,Yukio Aoyama,Hiroyuki Hayashi,Hiroki Nagase,Takahiro Ueno,Noboru Fukuda,Yoshiaki Matsumoto
Journal of Biomedicine and Biotechnology , 2012, DOI: 10.1155/2012/715928
Abstract: A simple and robust method using high-performance liquid chromatography with UV detection was developed and validated for the determination of six pyrrole-imidazole (PI) polyamides (HN.49, TGF-β1f, TGF-β1t, HN.50f, HN.50t, and LOX-1) in rat plasma. After the plasma proteins were precipitated with methanol containing phenacetin as an internal standard, the analytes were separated on a Luna C18 (2) (5 μm, 4.6×150 mm). Calibration curves were linear over the range of 0.5 to 200 μg/mL for HN.49, 0.25 to 200 μg/mL for TGF-β1f, TGF-β1t, HN.50t, and LOX-1, 1 to 200 μg/mL for HN.50f in rat plasma. The inter- and intraday precision were below 15%, and the accuracy was within 15% at the quality controls. The validated method was successfully applied to sample analysis for the pharmacokinetic study.
Retroperitoneoscopic radical nephrectomy with a small incision for renal cell carcinoma: Comparison with the conventional method
Hiroki Ito, Kazuhide Makiyama, Takashi Kawahara, Futoshi Sano, Takayuki Murakami, Narihiko Hayashi, Yasuhide Miyoshi, Noboru Nakaigawa, Masahiro Yao, Yoshinobu Kubota
Journal of Negative Results in BioMedicine , 2011, DOI: 10.1186/1477-5751-10-11
Abstract: Among the cases of T1N0M0 suspicious renal cell carcinoma treated at Yokohama City University between May 2003 and June 2009, the A method was performed in 51 cases and the B method was performed in 33 cases. The factors in the outcomes compared between the A and B methods were the duration of procedure, volume of bleeding, volume of transfusion, weight of the specimen, incidence of peritoneal injury, rate of conversion to open surgery, and perioperative complications.The duration of the procedure was 214.4 ± 46.9 minutes in the A method group and 208.1 ± 36.4 minutes in the B method group (p = 0.518). The volume of bleeding and the weight of the specimen were 105.5 ± 283.2 ml and 335.1 ± 137.4 g in the A method group and 44.8 ± 116 ml (p = 0.247) and 309.2 ± 126 g (p = 0.385) in the B method group. There was no significant difference in all factors analyzed.The A method would be highly possible to produce stable results, even during the introduction period when the staff and the institution are still unfamiliar with the retroperitoneoscopic surgery.The technical progress in laparoscopic surgery for renal cell carcinoma has been remarkable. Many institutions have introduced laparoscopic radical nephrectomy for renal cell carcinoma and even retroperitoneoscopic radical nephrectomy for renal cell carcinoma [1]. In recent years, these surgical methods are in widespread use, and the number of reports [2,3] about complications associated with surgery is rising. It has become important to identify how such a surgery can be completed in a safe manner during the introduction period when institutions and staff are still unfamiliar with these surgical methods.When retroperitoneoscopic radical nephrectomy for renal cell carcinoma was introduced into our institution, we performed a combined small skin incision method in our hospital. That's because we thought that the combined small skin incision method was safer than the conventional method that all procedures were performed w
An operative case of hepatic pseudolymphoma difficult to differentiate from primary hepatic marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue
Michihiro Hayashi, Noboru Yonetani, Fumitoshi Hirokawa, Mitsuhiro Asakuma, Katsuhiko Miyaji, Atsushi Takeshita, Kazuhiro Yamamoto, Hironori Haga, Takayuki Takubo, Nobuhiko Tanigawa
World Journal of Surgical Oncology , 2011, DOI: 10.1186/1477-7819-9-3
Abstract: A primary hepatic lymphoma (PHL) is defined as lymphoma localized and limited in the liver [1], not the secondary involvement of high- or intermediate grade non-Hodgkin's lymphoma, and accounts for less than 1% of all extranodal lymphomas [2]. Among them, a primary hepatic low-grade marginal zone B cell lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma) is extremely rare.On the other hand, hepatic pseudolymphoma (HPL), also termed as reactive lymphoid hyperplasia, or nodular lymphoid lesion, is extremely rare disease and characterized by the proliferation of non-neoplastic, polyclonal lymphocytes forming follicles with an active germinal center [3], and most importantly, is mimicking clinicopathologically to low grade lymphoma including MALT lymphoma.The etiology, pathogenesis and clinical implications of these two diseases remain unknown to a large extent. Reported underlying liver diseases include chronic viral hepatitis, autoimmune liver diseases, etc [4].Since clinical diagnosis is often difficult especially at its earlier stage, surgical resection appears a mainstay for diagnostic/therapeutic purpose.We herein present a laparoscopically operated case of hepatic pseudolymphoma which was difficult to differentially diagnose from primary hepatic MALT lymphoma, and discuss the clinicopathological features and clinical implications of these two disease entity.In April 2009, a 56-year-old Japanese woman was pointed out to have a space occupying lesion in the lateral segment of the liver on abdominal ultrasonography during health examination. Her social and family history was noncontributory and she had a previous medical history of appendectomy for acute appendicitis and laparoscopic cholecystectomy for cholecystolithiasis. She showed no abnormal physical findings, including lymphadenopathy and hepatosplenomegaly.Laboratory findings of blood examination were almost normal, including blood cell counts and differentiation, serochemical tests including liver
Comprehensive Analysis of Prokaryotes in Environmental Water Using DNA Microarray Analysis and Whole Genome Amplification
Takeshi Akama,Akira Kawashima,Kazunari Tanigawa,Moyuru Hayashi,Yuko Ishido,Yuqian Luo,Akihisa Hata,Noboru Fujitani,Norihisa Ishii,Koichi Suzuki
Pathogens , 2013, DOI: 10.3390/pathogens2040591
Abstract: The microflora in environmental water consists of a high density and diversity of bacterial species that form the foundation of the water ecosystem. Because the majority of these species cannot be cultured in vitro, a different approach is needed to identify prokaryotes in environmental water. A novel DNA microarray was developed as a simplified detection protocol. Multiple DNA probes were designed against each of the 97,927 sequences in the DNA Data Bank of Japan and mounted on a glass chip in duplicate. Evaluation of the microarray was performed using the DNA extracted from one liter of environmental water samples collected from seven sites in Japan. The extracted DNA was uniformly amplified using whole genome amplification (WGA), labeled with Cy3-conjugated 16S rRNA specific primers and hybridized to the microarray. The microarray successfully identified soil bacteria and environment-specific bacteria clusters. The DNA microarray described herein can be a useful tool in evaluating the diversity of prokaryotes and assessing environmental changes such as global warming.
On Canard Homoclinic of a Liénard Perturbation System  [PDF]
Makoto Hayashi
Applied Mathematics (AM) , 2011, DOI: 10.4236/am.2011.210170
Abstract: The classification on the orbits of some Liénard perturbation system with several parameters, which is relation to the example in [1] or [2], is discussed. The conditions for the parameters in order that the system has a unique limit cycle, homoclinic orbits, canards or the unique equilibrium point is globally asymptotic stable are given. The methods in our previous papers are used for the proofs.
Serum cystatin C levels to predict serum concentration of digoxin in Japanese patients
Tsutomu Nakamura, Takeshi Ioroi, Toshiyuki Sakaeda, Masanori Horinouchi, Nobuhide Hayashi, Kensuke Saito, Mitsuro Kosaka, Noboru Okamura, Keiichi Kadoyama, Shunichi Kumagai, Katsuhiko Okumura
International Journal of Medical Sciences , 2006,
Abstract: Cystatin C (Cys-C) has been recently paid great attention as a better endogenous marker of the glomerular filtration rate than creatinine (Cr). In this study, the usefulness of Cys-C was compared with Cr in terms of the estimation of the steady-state serum trough concentrations of digoxin in Japanese patients. Forty patients treated with digoxin and 56 healthy elderly subjects were participated in this study. The serum levels of Cys-C and Cr in the patients were higher than those in the healthy elderly subjects, but the increase of Cys-C was more predominant in the patients. Their levels were well-correlated for both of the healthy elderly subjects (r=0.691) and patients (r=0.774), but the serum concentrations of digoxin were better correlated with those of the reciprocal values of Cr (r=0.667) than those of Cys-C (r=0.383), presumably due to the fact that digoxin and Cr were excreted via both glomerular filtration and tubular secretion. Cys-C is useful for the substratification of the patients diagnosed to have normal renal function with Cr of < 1.3 mg/dL into those with normal and pseudo-normal renal function, resulting in the corresponding serum concentrations of digoxin.
High Sensitivity of Late Gadolinium Enhancement for Predicting Microscopic Myocardial Scarring in Biopsied Specimens in Hypertrophic Cardiomyopathy
Tetsuo Konno, Kenshi Hayashi, Noboru Fujino, Yoji Nagata, Akihiko Hodatsu, Eiichi Masuta, Kenji Sakata, Hiroyuki Nakamura, Masa-aki Kawashiri, Masakazu Yamagishi
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0101465
Abstract: Background Myocardial scarring can be assessed by cardiac magnetic resonance imaging with late gadolinium enhancement and by endomyocardial biopsy. However, accuracy of late gadolinium enhancement for predicting microscopic myocardial scarring in biopsied specimens remains unknown in hypertrophic cardiomyopathy. We investigated whether late gadolinium enhancement in the whole heart reflects microscopic myocardial scarring in the small biopsied specimens in hypertrophic cardiomyopathy. Methods and Results Twenty-one consecutive patients with hypertrophic cardiomyopathy who were examined both by cardiac magnetic resonance imaging and by endomyocardial biopsy were retrospectively studied. The right interventricular septum was the target site for endomyocardial biopsy in all patients. Late gadolinium enhancement in the ventricular septum had an excellent sensitivity (100%) with a low specificity (40%) for predicting microscopic myocardial scarring in biopsied specimens. The sensitivity of late gadolinium enhancement in the whole heart remained 100% with a specificity of 27% for predicting microscopic myocardial scarring in biopsied specimens. Quantitative assessments of fibrosis revealed that the extent of late gadolinium enhancement in the whole heart was the only independent variable related to the microscopic collagen fraction in biopsied specimens (β = 0.59, 95% confident interval: 0.15 – 1.0, p = 0.012). Conclusions Although there was a compromise in the specificity, the sensitivity of late gadolinium enhancement was excellent for prediction of microscopic myocardial scarring in hypertrophic cardiomyopathy. Moreover, the severity of late gadolinium enhancement was independently associated with the quantitative collagen fraction in biopsied specimens in hypertrophic cardiomyopathy. These findings indicate that late gadolinium enhancement can reflect both the presence and the extent of microscopic myocardial scarring in the small biopsied specimens in hypertrophic cardiomyopathy.
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