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Urinary Neutrophil Gelatinase Associated Lipocalin as a Marker of Tubular Damage in Type 2 Diabetic Patients with and without Albuminuria  [PDF]
Abeer A. Al-Refai, Safaa I. Tayel, Ahmed Ragheb, Ashraf G. Dala, Ahmed Zahran
Open Journal of Nephrology (OJNeph) , 2014, DOI: 10.4236/ojneph.2014.41006
Abstract:

Background: Neuttrophil gelatinase associated lipocalin (NGAL) was shown to be a good marker for predicting acute kidney injury (AKI). Some recent reports demonstrated that NGAL may be an early biomarker for kidney affection in diabetic patients. The aim of this work is to investigate urinary NGAL (UNGAL) in type 2 diabetic patients with and without albuminuria. Methods: This study included 46 type 2 diabetic patients and 15 healthy age and sex matched individuals as the control group. Diabetic patients were divided into three groups according to urinary albumin excretion (UAE), normoalbuminuria, microalbuminuria and macroalbuminuria. UNGAL was measured in all populations and corrected to urinary creatinine to account for day to day variation in urine volume and transformed log. Comparison between 4 groups (control, normoalbuminuria, microalbuminuria and macroalbuminuria) was done. Results: Log UNGAL/Creatinine ratio showed significant difference when comparing control group (0.70 ± 0.58) versus normoalbuminuria (1.71 ± 1.06), microalbuminuria (1.57 ± 0.72) and macroalbuminuria (1.92 ± 0.63), however,

Urine neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute canine kidney injury
Ya-Jane Lee, Yu-Yen Hu, Yi-Shan Lin, Chun-Ting Chang, Fong-Yuan Lin, Min-Liang Wong, Hsu Kuo-Hsuan, Wei-Li Hsu
BMC Veterinary Research , 2012, DOI: 10.1186/1746-6148-8-248
Abstract: The canine NGAL gene was successfully cloned and expressed. Polyclonal antibodies against canine NGAL were generated and used to develop an ELISA for measuring NGAL protein in serum and urine samples that were collected from 39 dogs at different time points after surgery.AKI was defined by the standard method, namely a serum creatinine increase of greater than or equal to 26.5 μmol/L from baseline within 48 h. At 12 h after surgery, compared to the group without AKI (12 dogs), the NGAL level in the urine of seven dogs with AKI was significantly increased (median 178.4 pg/mL vs. 88.0 pg/mL), and this difference was sustained to 72 h.As the increase in NGAL occurred much earlier than the increase in serum creatinine, urine NGAL seems to be able to serve as a sensitive and specific biomarker for the prediction of AKI in dogs.Acute kidney injury (AKI) shows high mortality and occurs in patients undergoing both cardiac or non-cardiac surgery [1], and among those in an intensive care unit (ICU). However, due to the lack of a consensus definition of AKI severity, the early recognition of AKI has been difficult. In the past, the Acute Dialysis Quality Initiative (ADQI) group published RIFLE criteria in which the severity of AKI was graded into Risk, Injury and Failure based on changes in serum creatinine and urine output; using this approach chronic renal failure was classified into the Loss and End stage [2]. More recently, the Acute Kidney Injury Network (AKIN) modified these criteria to increase the sensitivity, and recommended that a small change in serum creatinine (more than 26.5 μmol/L from baseline) within 48 h be recognized as stage I AKI [3]. Despite the lack of gold standard criteria for AKI in veterinary medicine, the application of the human criteria has been verified as applicable to dogs with AKI [4,5].Based on the consensus definition, the prediction of AKI has been improved. At present, AKI is mainly diagnosed based on an elevation in serum creatinine; howe
Lipocalin 2 acts as a cytoprotective factor against cisplatin toxicity, an in vitro study
Habibi Roudkenar M.,Ghasemipour Z.,Halabian R.,Mohammadi Roushandeh A.
DARU : Journal of Pharmaceutical Sciences , 2008,
Abstract: Background and the purpose of the stud:, Lipocalin 2 (Lcn2, NGAL) is a member of the lipocalin super family which has been known as an early marker for ischemic acute renal injury and cisplatin nephrortoxicity. In this study the ability of Lcn2 to prevent cisplatin-induced toxicity was studied. Methods: Lcn2 cDNA was isolated from Hep G2 cell line and cloned to pcDNA3.1(+) vector. The construct was trasnfected to CHO cell line. Stable clones were generated and the expression of Lcn2 was determined by RT-PCR and ELISA. Lcn2 gene in A549 cell line was also down-regulated with the siRNA. CHO and A549 cells were exposed to cisplatin and cell proliferation was determined by MTT assay. Results: Cell proliferation was higher in CHO expressing Lcn2 at doses of 75, 150, and 200 μg/ml of cisplatin after 48 h compared with control. Cisplatin toxicity increased significantly in A549 cells following treatment with Lcn2 siRNA. Major conclusion: findings of this study revealed that Lcn2 acts as a cytoprotective factor against cisplatin toxicity and suggests that Lcn2 might have the potential application to ameliorate the cisplatin toxicity.
Study of Lipocalin-2 Associated with Neutrophilic Gelatinases (uNGAL) in the Urine in Children with the Microbial Inflammatory Diseases of Kidneys and Urinary Tract  [PDF]
E. D. Bondarenko, A. V. Eremeeva, V. V. Dlin, A. A. Korsunsky, S. B. Orekhova, S. Gurbanova
Journal of Biosciences and Medicines (JBM) , 2015, DOI: 10.4236/jbm.2015.312004
Abstract:

Purpose of the study: Research of the clinical and diagnostic significance of determination of Lipocalin-2 associated with neutrophilic gelatinases (uNGAL) in the urine of children with urinary tract infection (UTI) and pyelonephritis. Materials and methods: We examined 30 children with acute pyelonephritis and UTI aged 1 to 16 years (average age 7.32 ± 4.52) including 26 girls and 4 boys. Verification of the diagnosis was conducted on the basis of clinical and laboratory data, medical history and instrumental examination of patients. All children were divided into 2 groups: 1st group—15 children with acute pyelonephritis, 2nd group—15 children with urinary tract infection. uNGAL was measured in the urine by enzyme-linked immunosorbent assay (EISA) (BioVendor Laboratoty Medicine). Results: It is found, that the urine level of NGAL depends on the damage degree of renal parenchyma. The correlation of medium strength was found between the excretion level of uNGAL during the acute period of pyelonephritis and the detection of renal scars according to the DMSA-nephroscintigraphy data. In the group of children with the acute pyelonephritis the direct correlation of medium strength was found between the excretion level of uNGAL/creatinine and leukocytosis value and also with the CRP blood level. Conclusion: The results allow us to recommend the determination of the excretion level of uNGAL/creatinine as an additional non-invasive marker for the early detection of renal parenchyma injury.

质粒制备和反应温度对嵌套缺失技术的影响
王子良,许丽艳,李恩民
癌变·畸变·突变 , 2005,
Abstract: ?背景与目的:探讨影响嵌套缺失反应的重要因素,找出相应的解决方案。材料与方法:以构建好的中性粒细胞明胶酶相关脂质运载蛋白(neutrophilgelatinaseassociatedlipocalin,ngal)基因5’端调控区质粒pglb-g6为研究对象,采用碱裂解法、聚二乙醇纯化法及qiagen试剂盒等3种不同方法制备该质粒,分别研究exoⅲ对它们进行非特异性切割的敏感性,选出对exoⅲ不敏感的质粒制备方法;在22℃和37℃分别进行缺失反应,琼脂糖凝胶电泳监控,以确定缺失反应是否已经发生;应用pcr技术与限制性内切酶酶切相结合的方法鉴定缺失子。结果:qiagen试剂盒制备的质粒对exoⅲ的非特异性切割最不敏感,可用于进行下一步的缺失反应;在22℃进行缺失反应时,电泳鉴定发现dna片段的长度没有明显的变短,而在37℃时,同一反应体系的dna片段长度明显缩短;进一步用限制性内切酶酶切可筛除pcr中的假阳性,获得准确的缺失子。结论:质粒的质量是确保进行特异性缺失反应的关键因素,通过qiagen试剂盒制备的质粒质量较好;设立一个37℃缺失对照管可作为判断低温条件下是否发生缺失的标准;pcr技术与限制性内切酶酶切相结合的方法鉴定缺失子,可确保获得特异的缺失子。
Znaczenie markera NGAL w diagnostyce powik ań nerkowych w przebiegu chorób kardiologicznych
Grzegorz Cichosz,Micha? Kidawa
Polish Journal of Cardiology , 2011,
Abstract: Wprowadzenie do diagnostyki nowych biomarkerów uszkodzenia kanalików nerkowych: NGAL (neutrophil gelatinase associated lipocalin), KIM-1 (kidney injury molecule-1), bogatego w cystein bia ka 61, cystatyny C, interleukiny-18 i innych bia ek (1-6) umo liwia bardzo wczesne rozpoznanie ostrej niewydolno ci nerek. W zwi zku z cz stymi powik aniami nerkowymi, spowodowanymi np. pokontrastowym uszkodzeniem nerek, oraz ze znan zale no ci mi dzy schorzeniami kardiologicznymi i wywo an przez nie dysfunkcj nerek rozpocz to próby zaadaptowania kilku dobrze scharakteryzowanych markerów do wczesnej diagnostyki uszkodzenia nerek u pacjentów leczonych z powodu chorób uk adu kr enia. Spo ród du ej grupy znanych markerów NGAL wykazuje si najwy szym znaczeniem diagnostycznym ze wzgl du na bardzo szybki okres reakcji na uszkodzenie kanalików. Jest to szczególnie istotne z powodu nieustannego skracania pobytu pacjenta po zabiegu w szpitalu.
The Predictivity of Neutrophil Gelatinase Associated Lipocaline in the Development of Radiocontrast-Induced Nephropathy in the Intensive Care Unit Patients
Türkmen, Funda,Berber, ?brahim,I??tmangil, Gülbu,Sakin, Mustafa
The Turkish Nephrology, Dialysis and Transplantation Journal , 2013, DOI: 10.5262/tndt.2013.1002.05
Abstract: OBJECTIVE: The aim of this study is to investigate the predictive value of neutrophil gelatinase associated lipocaline (NGAL) levels in the development of radiocontrast-induced nephropathy (rin) in intensive care unit patients.MATERIAL and METHODS: Forty patients (female: male was 23:17) with a mean age of 73.7± 9.7 yrs (range= 60-94 yrs) were included in the study. Lopromide 623mg/dl (ultravist 300) (iv) was administered at a dose of 1.5 ml/kg. NGAL measurements were performed with the ELISA method on serum samples (cut-off >25 ng/ml).RESULTS: Eight patients were diagnosed as RIN. Five of these had 20% or more increases in NGAL levels. Cases who developed RIN had a statistically significant and direct correlation between the increases in NGAL and serum creatinine levels (p= 0.02). When the means of NGAL levels were compared before and six hours after the procedure, there was a significant increase after the procedure (p<0.001). For 32 patients who did not develop RIN, there was no statistically significant difference between NGAL levels before and six hours after the procedure (p>0.05).CONCLUSION: When compared to creatinine, plasma NGAL levels help to establish the diagnosis of RIN at a much earlier stage.
NGAL在急性药物相关性间质性肾炎中的表达及其意义
张建国,丁涵露,任姜汶,高闻达
第三军医大学学报 , 2005,
Abstract:
ngal基因在永生化食管上皮细胞恶性转化中过表达的研究
许丽艳?,李恩民?,熊华淇?,蔡唯佳?,沈忠英?
生物化学与生物物理进展 , 2001,
Abstract: 为研究ngal(neutrophilgelatinase-associatedlipocalin)基因在永生化食管上皮细胞恶性转化中的表达情况,以永生化食管上皮细胞系shee和食管癌细胞系sheec互为对照,用cdna微列阵进行筛选,用rna印迹和rt-pcr进行鉴定,cdna克隆测序后与genbank进行blast分析比较.结果表明ngal基因在sheec中出现显著差异过表达,其cdna序列与小鼠24p3、大鼠nrl(neu-relatedlipocalin)、人中性粒细胞ngal和卵巢癌ngal具有较高的相似性.这提示ngal基因在永生化食管上皮细胞恶性转化中可能发挥着重要作用,可能是一种新的癌基因或促癌基因.
Neutrophil gelatinase-associated lipocalin in dehydrated patients: a preliminary report
Constantine N Antonopoulos, Alexandros Kalkanis, Georgios Georgakopoulos, Theodoros N Sergentanis, Dimitrios N Rigopoulos
BMC Research Notes , 2011, DOI: 10.1186/1756-0500-4-435
Abstract: A total of twelve patients presented with symptoms of mild dehydration defined by history of diarrheas or vomiting and orthostatic (postural) hypotension and an age and sex matched group of twelve control patients were included. The two groups of patients did not seem to differ in basic clinical and laboratory parameters. Serum Ngal was higher in dehydrated patients when compared to control group (Ngal = 129.4 ± 25.7 ng/mL vs 60.6 ± 0.4 ng/mL, p = 0.02). Ngal was not correlated with age, hemoglobin, white blood cell count, red blood cell count, urea or creatinine.The presence of elevated Ngal levels in dehydrated patients may suggest its role as a very sensitive biomarker in even minimal and "silent" prerenal kidney dysfunctionRenal impairment may be the result of a variety of renal or systemic diseases and may lead to renal failure [1]. Although the gold standard of renal failure's diagnosis is serial measurements of serum creatinine (Cr) [2,3], this biomarker is of little clinical importance in very early stages of renal disease. A novel biomarker, neutrophil gelatinase-asssociated lipocalin (Ngal), has been promising in evidencing renal impairment, even when changes in serum Cr level are undetectable [1].In order to explore the possible role of Ngal in subclinical renal dysfunction, such as renal hypoperfusion, we prospectively evaluated twelve patients aged>18 years with medical history suggesting mild dehydration, accompanied by orthostatic hypotension. A group of twelve apparently healthy individuals with no history of dehydration and no evidence of orthostatic hypotension were treated as controls. Ethical approval was obtained by the Ethics Committee of the "401 General Army Hospital" in Athens, Greece and all patients gave informed consent.Dehydrated and controls were similar in age and gender (75% males in each group) and in terms of basic laboratory tests (Hemoglobin; Hb, White blood cells; Wbc, Red blood cells; Rbc, Urea; Ur and Cr). Although no laborator
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