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Search Results: 1 - 10 of 176829 matches for " Natoniel Franklin de Melo "
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Chromosome study in Schistocerca (Orthoptera-Acrididae-Cyrtacanthacridinae): karyotypes and distribution patterns of constitutive heterochromatin and nucleolus organizer regions (NORs)
Souza, Maria José de;Melo, Natoniel Franklin de;
Genetics and Molecular Biology , 2007, DOI: 10.1590/S1415-47572007000100011
Abstract: chromosome analyses were performed in two grasshopper species of the genus schistocerca, s. pallens and s. flavofasciata. both species shared the same diploid number (2n = 23, x in males; 2n = 24, xx in females);and a conserved karyotype composed exclusively of acrocentric chromosomes, but differed in their distribution patterns of constitutive heterochromatin and nucleolus organizer regions (nors). constitutive heterochromatin was located in the pericentromeric region of all chromosomes in both species. s. flavofasciata presented an additional c-band on the distal region of the long arm of a small autosome pair (s9). nucleolus organizer regions (nors), revealed by silver nitrate staining (ag-nors), were observed on a medium autosome pair (m5) in both species. s. pallens presented an additional nor-bearing autosome (m6). the same sites were labeled after fish with an rdna probe in s. pallens cells.
Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta)
Ambrósio, Sandra Tereza;Melo, Natoniel Franklin de;
Acta Botanica Brasilica , 2004, DOI: 10.1590/S0102-33062004000400011
Abstract: the present work reports the effect of different ph and sucrose concentrations on in vitro propagation of nephrolepis biserrata. fronds aseptically obtained from stolon segment culture were cultivated in ms semi-solid medium supplemented with 0, 15, 30, 45 and 60g.l-1 sucrose and ph adjusted to 3, 5, 7 and 9, in a factorial design. frond number and length, pinnae number, raquis length and diameter, fresh and dry matter weight were measured. inhibition of shoot and leaf regeneration was observed in all the ph treatments in the absence of sucrose. on the other hand, when sucrose was added to the medium, the shoot number increased, reaching the maximum average values of 51.25 and 38.25 shoot per explant at ph 5 and 7, respectively. sucrose concentrations from 15 to 45g.l-1 increased leaf length and diameter and the ph 9 did not affect the dry matter weight, and was still not adequate for development of new fronds. young sporophytes were successfully acclimated.
Immature Embryo Rescue and in Vitro Development Evaluation of Intraspecific Hybrids from Brazilian Seedless Grapevine “Superior × Thompson” Clones  [PDF]
Eiryanne Fonseca de Menezes, Eliene Matos e Silva, Adriana Mayumi Yano-Melo, Patrícia Coelho de Souza Le?o, Natoniel Franklin de Melo
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.513209

The fruit production for export is an economically significant activity in the Valley of S?o Francisco River, especially in the irrigated lands of Petrolina-PE/Juazeiro-BA, Brazil. The development of new genetic material most suitable to the tropical climate and the demands of the consumer market have led to the selection of new seedless grapes cultivars. In this case, the use of the embryo rescue technique has produced satisfactory results for obtaining such materials, especially in the semiarid region. This study aimed to evaluate the in vitro development of intraspecific hybrids of grapevine (Vitis vinifera L.), derived from the rescue of immature embryos resultant from the crossing of “Superior Seedless” and “Thompson Seedless” Brazilian clones. To establish and develop the cultivation, the culture media was supplemented with 30 g/L sucrose, 0.1 g/L myo-inositol, 0.002 g/L glycine, 0.1 mg/L indoleacetic acid (IAA), 6.5 g/L of agar, adjusted pH to 5.7. The experiment was evaluated after 90 days. The variables measured were: number of nodes, number of leaves, plant height (cm), number of roots and length (cm) of the root system and internodes. The period of 60 days of in vitro culture of ovules resulted in the highest values of embryos (about 50%), as well as better characterized developmental stages with higher germination (47.3%). The three types of hybrid grapes evaluated in micropropagation showed very similar values of the measured parameters, even having originated from embryos of different developmental stages.

High levels of chromosomal differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae)
Moura, Rita de Cássia de;Melo, Natoniel Franklin de;Souza, Maria José de;
Genetics and Molecular Biology , 2008, DOI: 10.1590/S1415-47572008000300007
Abstract: euchroma gigantea was karyotypically studied using conventional staining, c-banding, silver nitrate staining and ribosomal fluorescent in situ hybridization (rdna fish). broad wide autosomal polymorphism and a complex sex determination system were found in this beetle. karyotype complements ranging from 2n = 32, x1x2x3y1y2y3 to 2n = 36,x1x2x3y1y2y3 were detected in the sample analyzed. punctiform supernumerary chromosomes were present in the different karyotypes. the karyotypic evolution of brazilian e. gigantea may have taken two directions, reduction in the diploid number of 2n = 36 to 24 through centric fusions or 2n = 24 to 36 due to chromosomal fissions. in addition, pericentric inversions were also involved. the complex multiple sex mechanism of this species seems to be old and well established since it is found in specimens from different populations. small pericentromeric blocks of constitutive heterochromatin were located on the autosomes and terminal blocks were also found on some small pairs. the sex chromosomes showed larger constitutive heterochromatin blocks. silver nitrate staining during prophase i of meiosis showed labeling of the sex chromosome chain. however, the rdna sites could only be precisely determined by fish, which permitted the identification of these ribosomal sites on chromosomes x1 and x2 of this species.
Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae)
Rocha, Marília de Fran?a;Melo, Natoniel Franklin de;Souza, Maria José de;
Genetics and Molecular Biology , 2011, DOI: 10.1590/S1415-47572011000200008
Abstract: the grasshopper species orthoscapheus rufipes and eujivarus fusiformis were analyzed using several cytogenetic techniques. the karyotype of o. rufipes, described here for the first time, had a diploid number of 2n = 23, whereas e. fusiformis had a karyotype with 2n = 21. the two species showed the same mechanism of sex determination (xo type) but differed in chromosome morphology. pericentromeric blocks of constitutive heterochromatin (ch) were detected in the chromosome complement of both species. cma3/da/dapi staining revealed cma3-positive blocks in ch regions in four autosomal bivalents of o. rufipes and in two of e. fusiformis. the location of active nors differed between the two species, occurring in bivalents m6 and s9 of o. rufipes and m6 and m7 of e. fusiformsi. the rdna sites revealed by fish coincided with the number and position of the active nors detected by agno3 staining. the variability in chromosomal markers accounted for the karyotype differentiation observed in the tribe abracrini.
Efeitos do paclobutrazol em diferentes concentra??es e períodos de imers?o na cultura do alho
Resende, Geraldo Milanez de;Costa, Nivaldo Duarte;Melo, Natoniel Franklin de;Souza, Rovilson José de;
Pesquisa Agropecuária Brasileira , 1999, DOI: 10.1590/S0100-204X1999000400015
Abstract: this study was carried out from april to september of 1989, in the experimental field of ufla, lavras, mg, brazil, with the objective of evaluating the influence of concentrations and periods of immersion of paclobutrazol on secondary growth control and marketable traits in garlic (allium sativum l.). the experimental design was a randomized complete block in a 4 x 5 factorial scheme, with four replications. the first factor consisted of paclobutrazol concentrations (0, 500, 1,000 and 1,500 mg l-1 of a.i.), and the second one of periods of immersion (30, 60, 90, 120 and 150 minutes). reduction in plant height and in number of leaves per plant from 60 to 90 days after planting with paclobutrazol applications was observed. mean bulb weight increased up to the concentration of 976 mg l-1 of paclobutrazol, and 451 mg l-1 gave the greatest percentage of bulbs of large size. there was no significant difference among treatments for total and commercial yields, percentage of secondary growth bulbs and number of cloves per bulb.
Avalia??o da capacidade de regenera??o in vitro em tomateiro industrial
Pesquisa Agropecuária Brasileira , 2000, DOI: 10.1590/S0100-204X2000000800004
Abstract: the objective of this work was to evaluate the regeneration capacity of the ipa-5 and ipa-6 brazilian industrial tomato (lycopersicon esculentum mill) cultivars using four compositions of culture media described in the literature and five inoculation methods. a new variation of inoculation, the split cotyledon method, was also tested. the largest frequency of shoot bud formation was 100% in the case of ipa-5 and 65% in the case of ipa-6. to induce shoot elongation, it was necessary to accomplish three subcultures of the explants presenting shoot buds. in the case of ipa-5, the number of the obtained shoots was higher when the induction of shoot buds was accomplished in culture medium containing bap (2.5 mg l-1) and iaa (0.2 mg l-1) followed by three subcultures on zeatin (0.5 mg l-1) containing medium. using such protocol, the ipa-5 cultivar produced, on the average, 5.45 elongated shoots through the split cotyledon method. that capacity exceeded significantly the trimmed cotyledon method, which produced 4.4 elongated shoots per explant. in the case of ipa-6, the best combination of culture medium and inoculation method produced 0.87 elongated shoot per explant. the elongated shoots were rooted and transferred to greenhouse.
Avalia o da capacidade de regenera o in vitro em tomateiro industrial
Pesquisa Agropecuária Brasileira , 2000,
Abstract: Este trabalho teve por objetivo avaliar a capacidade de regenera o das cultivares de tomateiro industrial (Lycopersicon esculentum Mill) IPA-5 e IPA-6, utilizando quatro composi es de meio de cultura descritos na literatura e cinco varia es de inocula o. Foi testada uma nova varia o de inocula o, denominada cotilédone fendido. A maior freqüência de forma o de gemas vegetativas foi 100% no caso de IPA-5, e 65% no caso de IPA-6. Para induzir o alongamento de brotos, foram necessários três subcultivos dos explantes apresentando gemas. No caso de IPA-5, o número de brotos obtidos foi maior quando a indu o de gemas foi realizada em meio contendo BAP (2,5 mg L-1) e AIA (0,2 mg L-1) seguido de três subcultivos, em meio como zeatina (0,5 mg L-1). Usando esse protocolo, a cultivar IPA-5 produziu uma média de 5,45 brotos alongados a partir do cotilédone fendido. Essa capacidade excedeu significativamente o cotilédone aparado, que produziu 4,4 brotos alongados por explante. No caso de IPA-6, a melhor combina o de meios e método de inocula o produziu 0,87 broto alongado por explante. Os brotos alongados foram enraizados e transferidos para casa de vegeta o.
Acclimatization of Tapeinochilos ananassae plantlets in association with arbuscular mycorrhizal fungi
Oliveira, Jo?o Ricardo Gon?alves de;Morais, Thiago Alberto de Lima;Melo, Natoniel Franklin de;Yano?Melo, Adriana Mayumi;
Pesquisa Agropecuária Brasileira , 2011, DOI: 10.1590/S0100-204X2011000900018
Abstract: the objective of this work was to assess the potential of three isolates of arbuscular mycorrhizal fungi to promote growth of micropropagated plantlets of tapeinochilos ananassae during acclimatization. the experiment was carried out in greenhouse, in a completely randomized block design, with four inoculation treatments: non?inoculated control and plants inoculated with glomus etunicatum, acaulospora longula or gigaspora albida, with ten replicates. after 90 days, the following parameters were evaluated: survival rate, height, leaf and tiller number, leaf area, fresh and dry biomass, contents of macro? and micronutrients in the root and shoot, glomerospore number, and mycorrhizal colonization. the survival percentage was 100%, except for plants inoculated with g. albida (80%). the isolate g. etunicatum is more suitable for plant development, since it improves survival, growth, dry matter production, nutritional status, and vigor of t. ananassae micropropagated plants.
Efeito do melado de cana-de-a?úcar no desenvolvimento in vitro de bananeira (Musa spp.) cv. Ma??
Ribeiro, Juliana Martins;Melo, Natoniel Franklin de;Coelho, ?ngela Katiussia Nascimento dos Santos;Pinto, Márcio dos Santos Teixeira;
Revista Ceres , 2012, DOI: 10.1590/S0034-737X2012000300001
Abstract: despite offering a number of advantages as the quality of final product, the technique of in vitro cultivation of plants is still considered expensive due to, among other things, the use of reagents with high purity for the preparation of nutrient media. among the alternatives that can be adopted to reduce the costs associated with the production of seedlings, the replacement of the pa product for those with lower has potential. based on this information, the purpose of this study was to evaluate the effect of molasses of sugar cane on in vitro development of banana cv. ma??. for this purpose, banana plants were inoculated in nutrient media made on the basis of sugarcane molasses, with four different concentrations (brix of 1.5, 3.0, 4.5 and 6.0) and data were compared with those of plants grown on ms medium (control), in a total of five treatments. despite plants have been developed in all treatments, it was observed that for the variables leaf number and fresh biomass, plants cultivated in ms medium (control) had higher performances. however, for the average number of roots, there was no statistical difference between treatments. the sugarcane molasses did not favor the development of banana plants, but can be used for in vitro rooting of these plants.
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