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Search Results: 1 - 10 of 2837 matches for " Nathalie Acevedo "
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New allergens of relevance in tropical regions: the impact of Ascaris lumbricoides infections
Luis Caraballo, Nathalie Acevedo
World Allergy Organization Journal , 2011, DOI: 10.1097/wox.0b013e3182167e04
Abstract:
Differential DNA Methylation in Purified Human Blood Cells: Implications for Cell Lineage and Studies on Disease Susceptibility
Lovisa E. Reinius, Nathalie Acevedo, Maaike Joerink, G?ran Pershagen, Sven-Erik Dahlén, Dario Greco, Cilla S?derh?ll, Annika Scheynius, Juha Kere
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041361
Abstract: Methylation of cytosines at CpG sites is a common epigenetic DNA modification that can be measured by a large number of methods, now even in a genome-wide manner for hundreds of thousands of sites. The application of DNA methylation analysis is becoming widely popular in complex disorders, for example, to understand part of the “missing heritability”. The DNA samples most readily available for methylation studies are derived from whole blood. However, blood consists of many functionally and developmentally distinct cell populations in varying proportions. We studied whether such variation might affect the interpretation of methylation studies based on whole blood DNA. We found in healthy male blood donors there is important variation in the methylation profiles of whole blood, mononuclear cells, granulocytes, and cells from seven selected purified lineages. CpG methylation between mononuclear cells and granulocytes differed for 22% of the 8252 probes covering the selected 343 genes implicated in immune-related disorders by genome-wide association studies, and at least one probe was differentially methylated for 85% of the genes, indicating that whole blood methylation results might be unintelligible. For individual genes, even if the overall methylation patterns might appear similar, a few CpG sites in the regulatory regions may have opposite methylation patterns (i.e., hypo/hyper) in the main blood cell types. We conclude that interpretation of whole blood methylation profiles should be performed with great caution and for any differences implicated in a disorder, the differences resulting from varying proportions of white blood cell types should be considered.
Particular characteristics of allergic symptoms in tropical environments: follow up to 24 months in the FRAAT birth cohort study
Nathalie Acevedo, Jorge Sánchez, Josefina Zakzuk, Adriana Bornacelly, Carlos Quiróz, álvaro Alvarez, Marta Puello, Ketty Mendoza, Dalgys Martínez, Dilia Mercado, Silvia Jiménez, Luis Caraballo
BMC Pulmonary Medicine , 2012, DOI: 10.1186/1471-2466-12-13
Abstract: Pregnant women representative of the low-income suburbs of the city were randomly screened for eligibility at delivery; 326 mother-infant pairs were included at baseline and biological samples were collected from birth to 24 months for immunological testing, molecular genetics and gene expression analysis. Pre and post-natal information was collected using questionnaires.94% of families were from the poorest communes of the city, 40% lacked sewage and 11% tap-water. Intestinal parasites were found as early as 3 months; by the second year, 37.9% of children have had parasites and 5.22% detectable eggs of Ascaris lumbricoides in stools (Median 3458 epg, IQR 975-9256). The prevalence of "wheezing ever" was 17.5% at 6 months, 31.1% at 12 months and 38.3% at 24 months; and recurrent wheezing (3 or more episodes) 7.1% at 12 months and 14.2% at 24 months. Maternal rhinitis [aOR 3.03 (95%CI 1.60-5.74), p = 0.001] and male gender [aOR 2.09 (95%CI 1.09 - 4.01), p = 0.026], increased risk for wheezing at 6 months. At 24 months, maternal asthma was the main predisposing factor for wheezing [aOR 3.65 (95%CI 1.23-10.8), p = 0.01]. Clinical symptoms of milk/egg allergy or other food-induced allergies were scarce (1.8%) and no case of atopic eczema was observed.Wheezing is the most frequent phenotype during the first 24 months of life and is strongly associated with maternal asthma. At 24 months, the natural history of allergic symptoms is different to the "atopic march" described in some industrialized countries. This cohort is representative of socially deprived urban areas of underdeveloped tropical countries. The collection of biological samples, data on exposure and defined phenotypes, will contribute to understand the gene/environment interactions leading to allergy inception and evolution.The causes of asthma and other allergic diseases remain unknown. Genetic and epidemiological studies suggest that for these multifactorial diseases the expression of different phenotypes de
Tratamiento de la enfermedad arterial periférica de las extremidades inferiores con células mononucleares de médula ósea autólogas: reporte de seguimiento a un a?o
Restrepo Múnera,Luz Marina; Hernández Cárdenas,Nathalie; Henao Pérez,Julieta; Cadavid Velásquez,Luis Gerardo; Jaramillo Velásquez,Sergio; Aguirre Acevedo,Daniel Camilo;
Iatreia , 2012,
Abstract: introduction: autologous bone marrow mononuclear cells have been shown to be safe and effective for treatment of patients with peripheral arterial disease (pad). angiogenesis can also be induced by growth factors synthesized by them. objective: to determine in colombia the feasibility, safety and outcome of the afore-mentioned treatment. methods: after informed consent, bone marrow was obtained by aspiration under local anesthesia; mononuclear cells were concentrated and their number and viability were established. they were suspended in saline solution and implanted by intramuscular injection into the gastrocnemius muscles of ischemic legs. control patients were left untreated. clinical evaluation included several parameters. flow cytometry was used for cell analysis. results: mean age of patients: 69 ± 11 years; cell viability: 99.15 ± 0.76%; total number of injected cells: 9.2 x 108 ± 6.2 x 108. after treatment, angiographic studies showed the formation of new collateral vessels in all patients, with minimal thickness increase. there were no complications from bone marrow aspiration and intramuscular administration of cells. all treated patients experienced increase in the walking distance and improvement of rest pain. conclusions: these preliminary results demonstrate that autologous cell therapy is safe, feasible and positively changes the natural history of patients with advanced peripheral arterial disease. in order to establish this treatment as a current practice in colombia, we suggest the study of a larger number of patients.
Proteomic and Immunochemical Characterization of Glutathione Transferase as a New Allergen of the Nematode Ascaris lumbricoides
Nathalie Acevedo, Jens Mohr, Josefina Zakzuk, Martin Samonig, Peter Briza, Anja Erler, Anna Pomés, Christian G. Huber, Fatima Ferreira, Luis Caraballo
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0078353
Abstract: Helminth infections and allergy have evolutionary and clinical links. Infection with the nematode Ascaris lumbricoides induces IgE against several molecules including invertebrate pan-allergens. These antibodies influence the pathogenesis and diagnosis of allergy; therefore, studying parasitic and non-parasitic allergens is essential to understand both helminth immunity and allergy. Glutathione transferases (GSTs) from cockroach and house dust mites are clinically relevant allergens and comparative studies between them and the GST from A. lumbricoides (GSTA) are necessary to evaluate their allergenicity. We sought to analyze the allergenic potential of GSTA in connection with the IgE response to non-parasitic GSTs. IgE to purified GSTs from Ascaris (nGSTA and rGSTA), house dust mites (rDer p 8, nBlo t 8 and rBlo t 8), and cockroach (rBla g 5) was measured by ELISA in subjects from Cartagena, Colombia. Also, multidimensional proteomic approaches were used to study the extract of A. lumbricoides and investigate the existence of GST isoforms. We found that among asthmatics, the strength of IgE levels to GSTA was significantly higher than to mite and cockroach GSTs, and there was a strong positive correlation between IgE levels to these molecules. Specific IgE to GSTA was found in 13.2% of controls and 19.5% of asthmatics. In addition nGSTA induced wheal and flare in skin of sensitized asthmatics indicating that it might be of clinical relevance for some patients. Frequency and IgE levels to GSTA were higher in childhood and declined with age. At least six GST isoforms in A. lumbricoides bind human IgE. Four isoforms were the most abundant and several amino acid substitutions were found, mainly on the N-terminal domain. In conclusion, a new allergenic component of Ascaris has been discovered; it could have clinical impact in allergic patients and influence the diagnosis of mite and cockroach allergy in tropical environments.
Adjuvant Treatment for High-Risk Operable Prostate Cancer  [PDF]
Bernard Paule, Nathalie Brion
Journal of Cancer Therapy (JCT) , 2010, DOI: 10.4236/jct.2010.11002
Abstract: Patients who have undergone a radical prostatectomy may have to face high risks of recurrence. The risk of recurrence is elevated due to probable occult metastatic disease at the time of diagnosis. A rationale for using multimodal approach in order to minimize the chance of disease recurrence and to improve the survival of high risk patients is emerging from preclinical and clinical studies. New molecular and genetics assays, may help to select patients most likely to benefit from these approaches. In this review, we will especially discuss the potential benefits of adjuvant therapy after radical prostatectomy. This paper presents the identification of these high-risk patients; the explanation of an adjuvant treatment of residual disease after a radical prostatectomy; the clinical studies with adjuvant androgen deprivation, radiotherapy and/or chemotherapy and the microarrays analysis. This review highlights the importance of these new adjuvant treatments that aims at targeting the factor which triggers metastatic disease following a radical prostatectomy
Therapeutic MUC1-Based Cancer Vaccine Expressed in Flagella-Efficacy in an Aggressive Model of Breast Cancer  [PDF]
Nathalie Machluf, Ruth Arnon
World Journal of Vaccines (WJV) , 2012, DOI: 10.4236/wjv.2012.23015
Abstract: MUC1, a tumor-associated antigen overexpressed in many carcinomas, represents a candidate of choice for cancer immunotherapy. Flagella-based MUC1 vaccines were tested in therapeutic setting in two aggressive breast cancer models, comprising the implantation of the 4T1-MUC1 cell line in either Balb/c, or Human MUC1 transgenic mice in which spontaneous metastases occurs. Recombinant flagella carrying only 7 amino acid of MUC1 elicited therapeutic activity, affecting both the growth of established growing tumors and the number of metastases. Higher therapeutic activity was achieved with an additional recombinant flagella designed with the SYFPEITHI algorithm. The vaccines triggered a Th1 response against MUC1 with no evident autoimmune response towards healthy MUC1-expressing tissues. Recombinant flagella carrying a 25-residue fragment of MUC1, induced the most effective response, as evidenced by a significant reduction of both the size and growth rate of the tumor as well as by the lower number of metastases, and expanding life span of vaccinated mice.
Lab-Scale Performance Evaluation of Vertical Flow Reed Beds for the Treatment of Chlorobenzene Contaminated Groundwater  [PDF]
Gerard Merlin, Nathalie Cottin
Journal of Environmental Protection (JEP) , 2012, DOI: 10.4236/jep.2012.328099
Abstract: Chlorinated Benzenes (CBs) that were released into the environment contaminate groundwater at many existing and former industrial sites. A research program was initiated to investigate the ecoremediation of CBs contaminated groundwater using subsurface flow constructed wetlands. Four lab-scale experiments were performed to evaluate re- moval efficiency with different operation conditions. The first experiment was achieved with two different solid-state materials: a peat and a lava stone (pozzolana). In order to stimulate biological activity, organic matter coming from an aged Vertical Flow Constructed Wetlands (VFCW) was added to the media. Mass balance was determined to assess the fate of these pollutants in this system. The biofiltres of the second experiment were constructed with the same materials but bioaugmentation was realized by adding organic matter of VFCW or by bacteria inoculums. Peat and pozzolana biofiltres planted with Phragmites australis constituted the third experiment to evaluate the effect of plants. Bioaugmen- tation was constituted by the addition of OM coming from aged VFCW. Compost mixed with pozzolana was the solid-state material of the fourth experiment. Columns were made of two stages. The first stage was unplanted and the second stage was planted with Phragmites. Peat has been replaced by compost, a renewable material. Lab-scale biofil- tres remove CBs with an efficiency of 70% - 99%, depending on studied media and conditions. Greater efficiency was observed with bioaugmented media. Volatilization was very low (<0.2%) and the detection of chlorides in water indi- cated the occurrence of biodegradation. The experiments have shown that organic solid-state materials (compost or peat) are useful for groundwater remediation, with higher treatment efficiency than pozzolana material. Bioaugmentation increased biological activity. Clogging of biofiltres have been observed and can be reduced by the presence of plants or by a resting period of 14 - 21 days (requiring alternative feedings on several filters).
Encoding of local and global cues in domestic dogs’ spatial working memory  [PDF]
Sylvain Fiset, Nathalie Malenfant
Open Journal of Animal Sciences (OJAS) , 2013, DOI: 10.4236/ojas.2013.33A001
Abstract: The current study investigated whether domestic dogs encode local and/or global cues in spatial working memory. Seven dogs were trained to use a source of allocentric information (local and/or global cues) to locate an attractive object they saw move and disappear behind one of the three opaque boxes arrayed in front of them. To do so, after the disappearance of the target object and out of the dogs’ knowledge, all sources of allocentric information were simultaneously shifted to a new spatial position and the dogs were forced to follow a U-shaped pathway leading to the hiding box. Out of the seven dogs that were trained in the detour problem, only three dogs learned to use the cues that were moved from trial to trial. On tests, local (boxes and experimenter) and/or global cues (walls of the room) were systematically and drastically shifted to a new position in the testing chamber. Although they easily succeeded the control trials, the three dogs failed to use a specific source of allocentric information when local and global cues were put in conflict. In discussion, we explore several hypotheses to explain why dogs have difficulties to use allocentric cues to locate a hidden object in a detour problem and why they do not differentiate the local and global cues in this particular experimental setting.
Public health perspectives on noise and cardiovascular disease  [PDF]
Lynnette-Nathalie Lyzwinski
World Journal of Cardiovascular Diseases (WJCD) , 2014, DOI: 10.4236/wjcd.2014.41005
Abstract:

Introduction: Emerging research suggests that noise impacts human health beyond simply the auditory system. There have been recent amendments to acceptable noise level thresholds within the occupational setting and new European Union directives for noise in the community from air and road sources. The purpose of this review will be to assess the relationship between noise and cardiovascular disease in different settings. Methods: A literature review was conducted using PubMed on noise and more cardiovascular disease endpoints. All studies published in the English language between 2000-present were included. Studies on noise annoyance were excluded. Results: There is a strong positive association between occupational noise exposure and cardiovascular outcomes. There is a moderate association with noise in the community setting from road sources and a weak association with aircraft noise. The strength of the association across settings is dependent on the outcome type, interaction with age and sex of subjects, and time of measurement. Road source noise had the strongest association with myocardial infarction, notably in women during night exposures. The association with hypertension and road noise, when stratified by sex, was significant in men during night-time exposures and in pregnant older women. Only nighttime aircraft noise was associated with statistically significant findings in adults. All sources of noise demonstrate a dose response relationship, which peaks at different noise thresholds. Occupational noise exposure had the strongest association with noise > 85 dB (A) and duration of exposure. Conclusion: There is sufficient evidence to warrant the precautionary public health principle to reduce noise at the population levels for industry workers through stronger legislation, regular compliance inspections, and health promotion. Where population wide noise legislation has not yet been enacted, noise exposure reduction at the individual level may be beneficial.

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