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Search Results: 1 - 10 of 14001 matches for " Nameirakpam Dolendro Singh "
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Complete Chloroplast Genome Sequence of an Orchid Model Plant Candidate: Erycina pusilla Apply in Tropical Oncidium Breeding
I-Chun Pan, Der-Chih Liao, Fu-Huei Wu, Henry Daniell, Nameirakpam Dolendro Singh, Chen Chang, Ming-Che Shih, Ming-Tsair Chan, Choun-Sea Lin
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0034738
Abstract: Oncidium is an important ornamental plant but the study of its functional genomics is difficult. Erycina pusilla is a fast-growing Oncidiinae species. Several characteristics including low chromosome number, small genome size, short growth period, and its ability to complete its life cycle in vitro make E. pusilla a good model candidate and parent for hybridization for orchids. Although genetic information remains limited, systematic molecular analysis of its chloroplast genome might provide useful genetic information. By combining bacterial artificial chromosome (BAC) clones and next-generation sequencing (NGS), the chloroplast (cp) genome of E. pusilla was sequenced accurately, efficiently and economically. The cp genome of E. pusilla shares 89 and 84% similarity with Oncidium Gower Ramsey and Phalanopsis aphrodite, respectively. Comparing these 3 cp genomes, 5 regions have been identified as showing diversity. Using PCR analysis of 19 species belonging to the Epidendroideae subfamily, a conserved deletion was found in the rps15-trnN region of the Cymbidieae tribe. Because commercial Oncidium varieties in Taiwan are limited, identification of potential parents using molecular breeding method has become very important. To demonstrate the relationship between taxonomic position and hybrid compatibility of E. pusilla, 4 DNA regions of 36 tropically adapted Oncidiinae varieties have been analyzed. The results indicated that trnF-ndhJ and trnH-psbA were suitable for phylogenetic analysis. E. pusilla proved to be phylogenetically closer to Rodriguezia and Tolumnia than Oncidium, despite its similar floral appearance to Oncidium. These results indicate the hybrid compatibility of E. pusilla, its cp genome providing important information for Oncidium breeding.
The complete chloroplast genome sequence of Citrus sinensis (L.) Osbeck var 'Ridge Pineapple': organization and phylogenetic relationships to other angiosperms
Michael G Bausher, Nameirakpam D Singh, Seung-Bum Lee, Robert K Jansen, Henry Daniell
BMC Plant Biology , 2006, DOI: 10.1186/1471-2229-6-21
Abstract: The complete chloroplast genome sequence of Citrus sinensis is 160,129 bp in length, and contains 133 genes (89 protein-coding, 4 rRNAs and 30 distinct tRNAs). Genome organization is very similar to the inferred ancestral angiosperm chloroplast genome. However, in Citrus the infA gene is absent. The inverted repeat region has expanded to duplicate rps19 and the first 84 amino acids of rpl22. The rpl22 gene in the IRb region has a nonsense mutation resulting in 9 stop codons. This was confirmed by PCR amplification and sequencing using primers that flank the IR/LSC boundaries. Repeat analysis identified 29 direct and inverted repeats 30 bp or longer with a sequence identity ≥ 90%. Comparison of protein-coding sequences with expressed sequence tags revealed six putative RNA edits, five of which resulted in non-synonymous modifications in petL, psbH, ycf2 and ndhA. Phylogenetic analyses using maximum parsimony (MP) and maximum likelihood (ML) methods of a dataset composed of 61 protein-coding genes for 30 taxa provide strong support for the monophyly of several major clades of angiosperms, including monocots, eudicots, rosids and asterids. The MP and ML trees are incongruent in three areas: the position of Amborella and Nymphaeales, relationship of the magnoliid genus Calycanthus, and the monophyly of the eurosid I clade. Both MP and ML trees provide strong support for the monophyly of eurosids II and for the placement of Citrus (Sapindales) sister to a clade including the Malvales/Brassicales.This is the first complete chloroplast genome sequence for a member of the Rutaceae and Sapindales. Expansion of the inverted repeat region to include rps19 and part of rpl22 and presence of two truncated copies of rpl22 is unusual among sequenced chloroplast genomes. Availability of a complete Citrus chloroplast genome sequence provides valuable information on intergenic spacer regions and endogenous regulatory sequences for chloroplast genetic engineering. Phylogenetic analyses
Release of Proteins from Intact Chloroplasts Induced by Reactive Oxygen Species during Biotic and Abiotic Stress
Kwang-Chul Kwon, Dheeraj Verma, Shuangxia Jin, Nameirakpam D. Singh, Henry Daniell
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0067106
Abstract: Plastids sustain life on this planet by providing food, feed, essential biomolecules and oxygen. Such diverse metabolic and biosynthetic functions require efficient communication between plastids and the nucleus. However, specific factors, especially large molecules, released from plastids that regulate nuclear genes have not yet been fully elucidated. When tobacco and lettuce transplastomic plants expressing GFP within chloroplasts, were challenged with Erwinia carotovora (biotic stress) or paraquat (abiotic stress), GFP was released into the cytoplasm. During this process GFP moves gradually towards the envelope, creating a central red zone of chlorophyll fluorescence. GFP was then gradually released from intact chloroplasts into the cytoplasm with an intact vacuole and no other visible cellular damage. Different stages of GFP release were observed inside the same cell with a few chloroplasts completely releasing GFP with detection of only red chlorophyll fluorescence or with no reduction in GFP fluorescence or transitional steps between these two phases. Time lapse imaging by confocal microscopy clearly identified sequence of these events. Intactness of chloroplasts during this process was evident from chlorophyll fluorescence emanated from thylakoid membranes and in vivo Chla fluorescence measurements (maximum quantum yield of photosystem II) made before or after infection with pathogens to evaluate their photosynthetic competence. Hydrogen peroxide and superoxide anion serve as signal molecules for generation of reactive oxygen species and Tiron, scavenger of superoxide anion, blocked release of GFP from chloroplasts. Significant increase in ion leakage in the presence of paraquat and light suggests changes in the chloroplast envelope to facilitate protein release. Release of GFP-RC101 (an antimicrobial peptide), which was triggered by Erwinia infection, ceased after conferring protection, further confirming this export phenomenon. These results suggest a novel signaling mechanism, especially for participation of chloroplast proteins (e.g. transcription factors) in retrograde signaling, thereby offering new opportunities to regulate pathways outside chloroplasts.
Active Deformations at the Churachandpur Mao Fault (CMF) in Indo Burma Ranges: Multidisciplinary Evidences  [PDF]
Arun Kumar, Manichandra Sanoujam, Laishram Sunil, Thingujam Dolendro
International Journal of Geosciences (IJG) , 2011, DOI: 10.4236/ijg.2011.24062
Abstract: Northeastern part of the Indian subcontinent is seismically active region with excessive rainfall and frequent landslides, which cause disruption of the road networks for couple of months in every year. The region has a typical morphotectonic setup where many active thrusts and faults have affected the landforms as well as the major part of the terrain. A prominent creeping strike-slip fault, named Chrachandpur Mao Fault (CMF), trending NS, is one of the triggering factors for frequent landslides, creeping low magnitude earthquakes. The life line of Manipur, national high way NH39 traverses through this fault in Manipur, hence the traffic is disrupted during the monsoon season. Based on the GPS campaign mode studies on western and eastern sides of the CMF, it is observed that there is a change in the crustal velocities from 16 - 22 mm/yr in east to 33 - 42 mm/yr in the west. Micro-deformations are also observed; the displacements along the vertical, N-S and E-W components are –0.111 mm/yr (downward), 0.03 mm/yr (north) and –0.011 mm/yr (west). The net displacement is 0.126 mm/yr with an azimuth of N 85? and dipping 13? towards west. Neotectonic development along the CMF with the GPS measurements suggest an aseismic nature of the fault with dextral component. Fault plane solutions of the earthquakes show northerly directed principal Paxis indicating the extension (T-axis) along eastwest. The resulting creeping of micro-deformation towards the western slopes of the terrain is aligned with the principal Taxis. The creeping triggers the microseismicity as well as the landslides along the CMF.
BIOMIMETIC SYNTHESIS OF SILVER NANOPARTICLES FROM AN ENDOPHYTIC FUNGUS AND THEIR ANTIMICROBIAL EFFICACY
Nirjanta Devi Nameirakpam,Shankar. P Dheeban,Sutha S.
International Journal of Biomedical and Advance Research , 2012, DOI: 10.7439/ijbar.v3i5.365
Abstract: In this work, an endophytic fungus, Penicillium sps. was isolated from the medicinal plant, Centella asiatica. The extracellular biosynthesis of silver nanoparticles using the filtrate of cell mass of an isolated Penicillium sps was monitored and the UV-Vis absorption spectrum recorded for the solution shows the characteristic surface plasmon resonance band for silver nanoparticles in the range of 390-440 nm. The SEM studies confirmed the formation of silver particles in the size of 100 nm, a clear indication of the formation of silver nanoparticles. The silver nano particle synthesised using filtrate of endophytic fungal biomass shows the antimicrobial effect on Proteus mirabilis, Shigella dysentriae, Klebsiella pneumoniae, and Staphylococcus aureus , and fungal pathogens such as Candida albicans. Finally it is revealed that nowadays the disease causing microbes have become resistant to drug therapy. So this environmental friendly method was analyzed to be faster when compared to other chemical methods and was evaluated as the very good choice of antimicrobial agent.
Analysis of Vortex Motion in Porous Media  [PDF]
Beant Singh, Chanpreet Singh
Journal of Electronics Cooling and Thermal Control (JECTC) , 2012, DOI: 10.4236/jectc.2012.22003
Abstract: In vortex motion fluid moves in curve path and the stream lines are curved. When the fluid flows between curved stream line, the centrifugal forces are setup which is counter balanced by pressure forces acting in the radial direction. The vortex motion has two types of curved motion. The first type called free vortex type, the fluid moves due to its own natural effect but energy is not added to fluid when moving in curved path. The second type called forced vortex, in which energy is added to fluid. A two dimensional steady laminar free convective flow of viscous incompressible flow in porous media voids is considered. In this paper the motion of fluid is studied while moving in the porous media. It is studied that the motion of the fluid in the porous media is vortex motion at the low Reynolds’s number, where the motion is laminar motion and the fluid obeys the laws of vortex motion.
Chromohysteroscopy—A new technique for endometrial biopsy in Abnormal Uterine Bleeding (AUB)  [PDF]
Nisha Singh, Bharti Singh
Open Journal of Obstetrics and Gynecology (OJOG) , 2013, DOI: 10.4236/ojog.2013.35A1003
Abstract:

Objective: To evaluate the role of chromohysteroscopy in improving diagnostic accuracy of endometrial biopsy in cases of AUB. Design: Cross sectional interventional study. Materials and Methods: This study was conducted on 60 women with AUB in Dept. of Obst. & Gyne at King George Medical University, Lucknow over a period of one year. All cases underwent diagnostic hysteroscopy followed by chromohysteroscopy using 2% methylene blue dye. Hysteroscopic guided biopsy was taken from stained and unstained areas followed by an endometrial aspiration biopsy from whole uterine cavity. The histopathology results of three samples were compared and analyzed in relation with staining pattern and type of AUB. Data analysis was done on SPSS version 15 of windows 2007. Results: Out of 60 cases, 11cases were found to have non hormonal pathology after chromohysterosopic biopsy. Eight (72.72%) cases were diagnosed by stained endometrial tissue, one (9.09%) by unstained tissue and three (27.27%) by endometrial aspiration. The diagnostic ability of stained tissue biopsy was significantly higher (p = 0.006) than unstained biopsy and endometrial aspiration. Conclusion: Chromohysteroscopy is a simple and effective technique for diagnosing endometrial pathology in cases of AUB.

Evaluation of EO-1 Hyperion Data for Crop Studies in Part of Indo-Gangatic Plains: A Case Study of Meerut District  [PDF]
Davinder Singh, Rajender Singh
Advances in Remote Sensing (ARS) , 2015, DOI: 10.4236/ars.2015.44021
Abstract: Due to the high number of bands in the hyperspectral image, the selection of optimum bands for crop classification is a prerequisite. The Hyperion sensor has 242 spectral bands out of which 143 useable bands were selected. The bands reflected wavelength from 400 to 1000 nm to the VNIR spectrometer and transmitted the band from 900 to 2500 nm to the SWIR spectrometer. Spectral Angle Mapping Classification (SAMC) approach and a multi-scale object oriented method are applied for crop studies. The result obtained from the accuracy assessment by comparing Ground Control Points (GCP) with the help of image spectra shows 78% of overall accuracy. This shows that these data are highly useful in studying the crop diversification.
How Indian CPI and Industrial Production Respond to Global Oil Price Shocks? Regime-Dependent Impulse Responses  [PDF]
Amanjot Singh, Rajdeep Singh
Theoretical Economics Letters (TEL) , 2017, DOI: 10.4236/tel.2017.75102
Abstract: For emerging markets like India, where around 80 percent of the crude requirements are met through imports, it is an imperative task to comprehend impact of global crude oil price shocks on Indian macroeconomic variables. The present study attempts to understand these asymmetric dynamic interactions between global crude oil price shocks and Indian macroeconomic variables by employing Markov switching-Vector Autoregressive (MS-VAR) regime-dependent impulse responses in level forms. The findings hold an important place in the wake of inflation targeting regime adopted by the monetary policy authorities. The findings highlight the existence of two regimes, namely lower and higher oil price variance regimes. The response of industrial production and consumer prices is different towards oil price shocks in different regimes. In the lower oil variance regime, there is negative (positive) relationship observed between crude oil shocks and industrial production (consumer prices). On the other hand, there is a positive equilibrium shift in the industrial production in the higher oil variance regime with cost pushing inflationary pressures in the long run. The findings bear strong implication for the policy makers in their attempt to combat effects of crude oil shocks. As per the findings, the emerging market policy makers should display a cautious approach during higher oil price volatile phases in order to support industrial production and consumer demand.
Erratum to “How Indian CPI and Industrial Production Respond to Global Oil Price Shocks? Regime-Dependent Impulse Responses” [Theoretical Economics Letters 7 (2017) 1511-1531]  [PDF]
Amanjot Singh, Rajdeep Singh
Theoretical Economics Letters (TEL) , 2017, DOI: 10.4236/tel.2017.77148
Abstract: The original online version of this article (Singh, A. and Singh, R. (2017) How Indian CPI and Industrial Production Respond to Global Oil Price Shocks? Regime-Dependent Impulse Responses. Theoretical Economics Letters, 7, 1511-1531. https://doi.org/10.4236/tel.2017.75102) was published without referring the source of Figure 1.
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