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Implications of the Hemodynamic Optimization Approach Guided by Right Heart Catheterization in Patients with Severe Heart Failure
Rohde, Luís E.;Furian, Thiago;Campos, Candice;Biolo, Andreia;Rabelo, Eneida;Foppa, Murilo;Clausell, Nadine;
Arquivos Brasileiros de Cardiologia , 2002, DOI: 10.1590/S0066-782X2002000300001
Abstract: objective: to report the hemodynamic and functional responses obtained with clinical optimization guided by hemodynamic parameters in patients with severe and refractory heart failure. methods: invasive hemodynamic monitoring using right heart catheterization aimed to reach low filling pressures and peripheral resistance. frequent adjustments of intravenous diuretics and vasodilators were performed according to the hemodynamic measurements. results: we assessed 19 patients (age = 48±12 years and ejection fraction = 21±5%) with severe heart failure. the intravenous use of diuretics and vasodilators reduced by 12 mm hg (relative reduction of 43%) pulmonary artery occlusion pressure (p<0.001), with a concomitant increment of 6 ml per beat in stroke volume (relative increment of 24%, p<0.001). we observed significant associations between pulmonary artery occlusion pressure and mean pulmonary artery pressure (r=0.76; p<0.001) and central venous pressure (r=0.63; p<0.001). after clinical optimization, improvement in functional class occurred (p< 0.001), with a tendency towards improvement in ejection fraction and no impairment to renal function. conclusion: optimization guided by hemodynamic parameters in patients with refractory heart failure provides a significant improvement in the hemodynamic profile with concomitant improvement in functional class. this study emphasizes that adjustments in blood volume result in imme-diate benefits for patients with severe heart failure.
Preditores clínicos de fra??o de eje??o preservada em insuficiência cardíaca descompensada
Goldraich, Livia;Clausell, Nadine;Biolo, Andréia;Beck-da-Silva, Luís;Rohde, Luís Eduardo;
Arquivos Brasileiros de Cardiologia , 2010, DOI: 10.1590/S0066-782X2010000300017
Abstract: background: identification and clinical impact of preserved left ventricular ejection fraction (lvef) on in-hospital outcomes in patients with acute decompensated heart failure (hf) remain poorly defined. objective: to describe clinical predictors and in-hospital outcomes of acute decompensated hf patients and preserved lvef, and to develop a clinically-based predictive rule based on data acquired on admission. methods: consecutive admissions for hf (n=721) at a tertiary care hospital were followed up to discharge or death. more than 80 clinical variables were evaluated to identify predictors of preserved lvef upon admission. results: preserved lvef (>50%) was identified in 224 (31%) hospitalizations. clinical predictors of preserved lvef were age > 70 years old (p=0.04), female gender (p<0.001), non-ischemic etiology (p<0.001), atrial fibrillation or flutter (p=0.001), anemia (p=0.001), pulse pressure > 45 mmhg (p<0.01) and absence of ekg conduction abnormalities (p<0.001). a clinical score based on these variables was accurate to predict preserved lvef upon hospital admission (area under roc curve of 0.76). no significant differences were observed on in-hospital mortality or clinical complications according to quintiles of lvef. conclusion: preserved lvef is a prevalent and morbid condition among hospitalized hf patients. simple clinical data obtained on admission might be useful for predicting preserved lvef.
Diálise peritoneal (DP) como tratamento da insuficiência cardíaca congestiva (ICC) em pacientes com doen?a renal cr?nica estágio IV
Prompt, Carlos Alberto;Almeida, Rafael de;Clausel, Nadine;Biolo, Andréa;Bercht, Fábio;Oliveira, Jer?nimo D. C.;Almeida, Eduardo D.;Pribbernow, Suzane;
Jornal Brasileiro de Nefrologia , 2009, DOI: 10.1590/S0101-28002009000300008
Abstract: congestive heart failure (chf) has a growing incidence, a great impact on public health, and high morbidity and mortality. excessive blood volume is a prevalent complication present in 80% of the patients diagnosed with chf. pharmacological and non-pharmacological strategies in the treatment of chf aim to better manage blood volume and reduce the use of diuretics. extracorporeal ultrafiltration has evidenced better weight control, and a reduction in the length of hospitalization and re-hospitalization of patients with chf. we report the cases of 2 patients diagnosed with chf and treated with peritoneal dialysis at hospital de clínicas de porto alegre.
Composi??o de algas perifíticas (exceto Bacillariophyceae) em distintos substratos naturais de um ambiente semilótico, planície de inunda??o do Alto Rio Paraná, Brasil
Biolo, Stefania;Rodrigues, Liliana;
Brazilian Journal of Botany , 2011, DOI: 10.1590/S0100-84042011000300006
Abstract: the purpose of this study was the taxonomy of dominant periphytic algae (except bacillariophyceae) of three natural substrates, eichhornia azurea kunth, nymphaea amazonum mart. & zucc. and oxycaryum cubense (poepp. & kunth) lye in a semi-lotic environment (the pau véio backwater) at the paraná river floodplain. only those taxa with a frequency of occurrence over 75% of all samples were considered, except diatoms. thirty-one taxa were illustrated, measured and identified at species level, a total of 31 taxa were described, distributed in classes: chlorophyceae (3), cryptophyceae (1), cyanobacteria (14 taxa), zygnemaphyceae (7), euglenophyceae (2) and xanthophyceae (4). twenty-three taxa are first taxonomic reports for the upper paraná river floodplain: chamaesiphon investiens skuja, geitleribactron subaequale (geitler) komárek, chroococcus limneticus lemmerm., c. minimus (keissler) lemmerm., c. minor (kütz.) n?geli, aphanothece microscopica n?geli, phormidium molle (kütz.) gomont, leptolyngbya angustissima (west & g.s. west) anagn. & komárek, l. foveolarum (rabenhorst ex gomont) anagn. & komárek and pseudanabaena frigida (fritsch) anagn. (cyanobacteria); characium ensiforme hermann, c. ornithocephalum a. braun and desmodesmus brasiliensis (bohlin) e. hegewald (chlorophyceae); cosmarium abbreviatum racib., c. bireme nordst., c. pseudopyramidatum lundell, c. subadoxum gr?nblad, c. trilobulatum reinsch, gonatozygon pilosum wolle and staurastrum forficulatum lundell (zygnemaphyceae); trachelomonas hispida (perty) stein emend. deflandre (euglenophyceae); cryptomonas tenuis pascher (cryptophyceae) and characiopsis sphagnicola pascher (xanthophyceae).
An Analysis of the Global Expression of MicroRNAs in an Experimental Model of Physiological Left Ventricular Hypertrophy
Nidiane C. Martinelli, Carolina R. Cohen, Kátia G. Santos, Mauro A. Castro, Andréia Biolo, Luzia Frick, Daiane Silvello, Amanda Lopes, Stéfanie Schneider, Michael E. Andrades, Nadine Clausell, Ursula Matte, Luis E. Rohde
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0093271
Abstract: Background MicroRNAs (miRs) are a class of small non-coding RNAs that regulate gene expression. Studies of transgenic mouse models have indicated that deregulation of a single miR can induce pathological cardiac hypertrophy and cardiac failure. The roles of miRs in the genesis of physiological left ventricular hypertrophy (LVH), however, are not well understood. Objective To evaluate the global miR expression in an experimental model of exercise-induced LVH. Methods Male Balb/c mice were divided into sedentary (SED) and exercise (EXE) groups. Voluntary exercise was performed on an odometer-monitored metal wheels for 35 days. Various tests were performed after 7 and 35 days of training, including a transthoracic echocardiography, a maximal exercise test, a miR microarray (miRBase v.16) and qRT-PCR analysis. Results The ratio between the left ventricular weight and body weight was increased by 7% in the EXE group at day 7 (p<0.01) and by 11% at day 35 of training (p<0.001). After 7 days of training, the microarray identified 35 miRs that were differentially expressed between the two groups: 20 were up-regulated and 15 were down-regulated in the EXE group compared with the SED group (p = 0.01). At day 35 of training, 25 miRs were differentially expressed: 15 were up-regulated and 10 were decreased in the EXE animals compared with the SED animals (p<0.01). The qRT-PCR analysis demonstrated an increase in miR-150 levels after 35 days and a decrease in miR-26b, miR-27a and miR-143 after 7 days of voluntary exercise. Conclusions We have identified new miRs that can modulate physiological cardiac hypertrophy, particularly miR-26b, -150, -27a and -143. Our data also indicate that previously established regulatory gene pathways involved in pathological LVH are not changed in physiological LVH.
Bench-to-bedside review: Lactate and the lung
Fulvio Iscra, Antonino Gullo, Gianni Biolo
Critical Care , 2002, DOI: 10.1186/cc1519
Abstract: The respiratory and immune functions of the lung are largely dependent on the activity of a number of metabolic pathways. Surfactants and prostanoids are synthesized from lipid precursors. Protein synthesis is maintained at a high rate to maintain a rapid turnover of the endothelial and parenchymal pulmonary cells and of the immune cells. Energy is produced from glucose, fatty acids and branched chain amino acid oxidation. Lactate, alanine and glutamine are synthesized to shuttle carbon and nitrogen residues derived from glucose and amino acid metabolism.Despite the importance of these metabolic pathways, the role of the lung in interorgan substrate exchange in physiological and pathological conditions is largely unknown. In humans, substrate exchange across an individual organ is determined according to the Fick principle, by measuring substrate arteriovenous concentrations and local blood flow. This approach has been largely used to determine skeletal muscle metabolism in the human limbs. In the lung, however, the arteriovenous difference of substrate concentrations is usually small compared with a high rate of blood flow through the tissue. This limits the ability of the Fick technique to detect statistically significant rates of substrate exchange across the lung in most circumstances.Virtually all tissues can synthesize or utilize lactate. Lactate is synthesized from the pyruvic acid derived from glycolysis, whereas it can be utilized to form glucose or it can be oxidized through pyruvate and the tricarboxylic acid cycle. In physiological conditions, lactate is mainly produced in the skin, skeletal muscle, leucocytes and red blood cells. It is mainly utilized, however, in the liver and the kidney. Lactate is therefore one of the major carbon shuttles among body tissues.In different conditions, the rate of lactate synthesis is dependent on the activity of the glycolytic pathway relative to the oxidative capacity of the pyruvate dehydrogenase enzymatic complex. A
Studying Heavy Metals in Sediments Layers along Selected Sites on the Lebanese Coast  [PDF]
Journal of Water Resource and Protection (JWARP) , 2010, DOI: 10.4236/jwarp.2010.21006
Abstract: Ensuring the environmental protection of the Lebanese coast requires a continues monitoring system. For this purpose, four heavy metals (Fe, Mn, Cu and Pb in the marine sediments along the Lebanese coast were selected for analysis Sampling was carried out from two sites: Beirut and Batroun. Thus, 1g of dried sample is used for digestion by wet mineralization in order to determine the concentration of the four heavy metals by atomic absorption spectrometry. The results showed that Beirut area is polluted, by Fe and Mn as well as the station Bat 2 of Batroun. For Cu and Pb, Batroun region is more polluted in the superficial layers. The analysis also showed significant difference between the sites except for Cu. A difference between depths and between particles size fractions are observed for all the parameters studied. There is no a significant difference in layer sequence except for the Pb, and neither between the repetitions of the same sample. Results showed that the values of the four metals studied do not exceed the maximum limits at both sites, but they showed increase in comparison with the analyses obtained before July 2006 conflict, which was caused by the release of large quantity of fuel-oil from Jiyeh Power Station.
Influence of production processes in quality of fermented milk "Laban" in Lebanon  [PDF]
Zeineddine Mayssoun, Nassif Nadine
Health (Health) , 2010, DOI: 10.4236/health.2010.24057
Abstract: Yoghurt (Laban) is one of the most consumed food products in Lebanon. Thus its quality has given a concern. In this study, the sensory, chemical and rheological properties of commercial and traditional samples were investigated in order to characterize this fermented milk. Hence, Laban samples were collected from 14 areas in Lebanon; especially from mountainous regions and from the capital Beirut. Forty-two samples were provided by processing industry whether at small, medium, or large scale. A statistical analysis was carried out, and thus sensory and physicochemical properties were subjected to two approaches of variance analysis. Pearson correlation coefficients between attributes were also calculated. Both, the analyses of variance and correlations were conducted using SPSS 3. The physicochemical analysis and the microbiological analysis exhibit a significant effect of the date, and the manufacturing process. Also, the instrumental data showed no significant correlation between physicochemical and microbiological parameters, which indicates that they are completely independent. Moreover, the general appreciation of descriptive sensory analysis of products display that this appreciation is not dependant on the production process. It is also noticed that some sensory characteristics can be dread by instrumental measures. This research endorses the essential role of quality control for the manufacturing of yoghurt in Lebanon.
Preoccupation with Weight and Eating Patterns of Lebanese and Cypriot Female Students  [PDF]
Ioanna Katsounari, Nadine Zeeni
Psychology (PSYCH) , 2012, DOI: 10.4236/psych.2012.36073
Abstract: This comparative cross-cultural study explores the prevalence of preoccupation with weight and eating patterns among female university students in two distinct cultural contexts, Cyprus and Lebanon. Data was collected by means of self-administered questionnaires to 200 students from each culture. To gather the data for this study, the Dutch Eating Behavior Scale was used for the assessment of eating behaviors. Body Mass Index (BMI) and Weight directed behavior were also calculated. The Pearson Product-Moment Correlation Coefficient was used to determine whether there is a linear relationship between students’ BMI and eating behaviors. Findings suggested that with respect to culture and pre-occupation with weight, Cypriot students are more pre-occupied with their weight. However, Lebanese students received higher emotional and external eating scores. In addition, a positive relationship between Body Mass Index, restrained and emotional eating was found in both cultures Also, in both cultures there was a significant positive relationship between restrained and emotional eating, emotional and external eating. These findings suggest that in the Lebanese culture, eating dysfunction among women may be due to responsiveness to external and emotional cues while in the Cypriot culture may be due to an over pre-occupation with weight fuelled by sociocultural agents. Understanding the underlying causes of eating deviations and the existing elements in each culture which promote these deviations could lead to better prevention efforts in two societies where the rise in eating disturbances has been alarming.
“We Are Only as Strong as We Are United, as Weak as We Are Divided” a Dynamic Analysis of the Peer Support Networks in the Harry Potter Books  [PDF]
Goele Bossaert, Nadine Meidert
Open Journal of Applied Sciences (OJAppS) , 2013, DOI: 10.4236/ojapps.2013.32024

This research studied the concept of enacted peer support during adolescence by means of the Harry Potter Series. A network approach was used. Results indicated the importance of reciprocity and transitivity for enacted peer support during adolescence. Contrary to our expectations, gender, age and personality traits did not affect enacted peer support. No homophily effects based on gender and age were detected. However, students were found to be more supportive of students with similar personality traits. We hope this study adds to the current knowledge on peer support in adolescence and promotes the use of social theories and methods in literacy research.

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