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Search Results: 1 - 10 of 11943 matches for " Molecular identification "
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The comparison of PCR technique and API-20E kit with the conventional biomedical methods for the
Soltan Dallal MM
Medical Laboratory Journal , 2012, DOI: http://www.goums.ac.ir/mljgoums/index.php?slc_lang=en&sid=1
Abstract: Background and objectives: Salmonella is one of the most importantagents of gastrointestinal infection and diarrhea in our country.Misdiagnosis of these bacteria leads to treatment failure. The aim of thisstudy was to make a comparison between PCR and the API-20E andconventional biochemical tests carried out for the identification ofSalmonella.Material and Methods: In this study, 470 specimens were taken fromchildren, with acute gastroenteritis, referred to teaching hospitals calledImam, Shariati and children medical centre. The specimens weretransferred to microbiology laboratory in public health school foridentification of Salmonella with PCR and API-20E methods.Results: Of 470 specimens, 65(13.8%) are positive for salmonella inhospital laboratory, while 37 (7.9%) for API-20E and 39 (8.3%) for PCRare positive. The results of antibiotic sensitivity tests on 39 salmonellaisolated from diarrhea specimens show that 73.3% of them are resistanceto at least one of the sixteen antibiotics tested.Conclusion: Based on the the results, there is significant difference(P<0.05) between conventional method, API-20E and PCRKey words: Salmonella, conventional identification, molecularidentification
Pathogenicity Assay and Molecular Identification of Fungi and Bacteria Associated with Diseases of Tomato in Malaysia  [PDF]
Tavga Sulaiman Rashid, Kamaruzaman Sijam, Hayman Kakakhan Awla, Halimi Mohd Saud, Jugah Kadir
American Journal of Plant Sciences (AJPS) , 2016, DOI: 10.4236/ajps.2016.76090
Abstract: This study was conducted in order to determine the fungi and bacteria associated with tomato plants at Cameron Highlands Malaysia. The fungi which have been isolated and detected from tomato plants were: Fusarium oxysporum, F. solani, F. acuminatum, Rhizoctonia solani, Colletotrichum boninense, C. acutatum and Phoma destructiva. The bacteria which have been isolated and detected from tomato plants were: Ralstonia solanacearum,Xanthomonas vesicatoria, X. gardneri and Pseudomonas syringae. While the most pathogenic fungi were C. boninense, P. destructive and F. oxysporum with the disease incidence (89.6%, 86.6%, 85.6%) respectively, the most pathogenic bacteria were X. vesicatoria
Identificación de una secuencia de ADN genómico de Leishmania especifica del subgénero Leishmania
Orué,Andrea; De Abreu,Nancy Y; Martínez,Clara; Mendoza-León,Alexis;
Revista de la Sociedad Venezolana de Microbiología , 2008,
Abstract: leishmania is the causal agent of the leishmaniasis disease. the different species of this protozoa parasite are grouped in two subgenera, viannia and leishmania, according to their development in the sandfly vector. a specific pcr assay, β500-pcr, has been developed for the viannia subgenus using the genomic β500 dna sequence. in the present work we present the isolation and identification of a genomic sequence of 280 bp, l280, obtained from genomic dna of leishmania (leishmania) mexicana after application of the β500-pcr assay at low stringency. after partial sequencing of l280 a pcr assay was generated, l280-pcr, this yielded a product of 260 bp at different conditions of stringency, when genomic dna of different species of leishmania subgenus was used. the l280-pcr assay was negative to genomic dna of species belonging to the viannia subgenus and also to other kinetoplastid organisms and human. the results suggest specificity of the l280-pcr assay for the leishmania subgenus.
Identifica??o molecular e suscetibilidade antifúngica de Candida parapsilosis isoladas no Ceará, Brasil
Menezes, Everardo Albuquerque;Vasconcelos Júnior, Ant?nio Alexandre de;Cunha, Francisco Afranio;Cunha, Maria Concei??o dos Santos Oliveira;Braz, Bárbara Helena Lima;Capelo, Ligya Guimar?es;Silva, Carlla Lorena Fa?anha;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2012, DOI: 10.1590/S1676-24442012000600005
Abstract: introduction: c. parapsilosis is the second or third most isolated yeast from blood cultures in various parts of the world. it is a commonly isolated pathogen in brazil and ceará. c. parapsilosis is liable to form biofilms on catheters and other medical devices, which contributes to the spread of this yeast. objective: the objective of this study was to identify and assess the antifungal susceptibility of c. parapsilosis isolates from blood and urine samples collected from patients in hospitals in ceará. methods: we isolated and identified 57 strains of c. parapsilosis. the strains were identified by phenotypic and molecular tests. the susceptibility to antifungals was assessed by broth microdilution (clinical and laboratory standards institute [clsi] protocol m27a3). we tested five antifungals (amphotericin b, caspofungin, fluconazole, itraconazole and voriconazole). results and conclusion: the strains were identified as c. parapsilosis by phenotypic tests and confirmed by molecular tests. as to the sensitivity profile, the strains were sensitive to the antifungal agents, hence resistance is still a rare phenomenon among c. parapsilosis isolates in ceará.
Varietal identification of coffee seeds by RAPD technique
Crochemore, Maria Lúcia;Nunes, Liliane Moreira;Andrade, Giselly Aparecida;Molinari, Hugo Bruno Correa;Vasconcellos, Maria Elizabeth;
Brazilian Archives of Biology and Technology , 2004, DOI: 10.1590/S1516-89132004000100002
Abstract: this study aimed the identification of cultivars and/or lines of coffea arabica of commercial interest, using pcr-rapd markers. the dna of ground seeds lots of 12 cultivars and/or lines were evaluated with five primers (operon opa 01, opa 04, opg 11, opy 16, and opx 09) were obtained from a selection of 56 primers. the electrophoretic profiles allowed distinction among eight cultivars and/or lines as well as heterogeneity between and within lots of iapar59.
Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level
Delamare, Ana Paula Longaray;Artico, Liane de Oliveira;Grazziotin, Felipe Gobbi;Echeverrigaray, Sergio;Costa, Sérgio Olavo Pinto da;
Brazilian Journal of Microbiology , 2002, DOI: 10.1590/S1517-83822002000400016
Abstract: fifteen well-defined strains of aeromonas of thirteen species were analyzed by sds protein electrophoretic analysis (sds-page) and random amplified polymorphic dna analysis (rapd). the comparison between the patterns obtained by both methods allowed differentiating all the strains. clusters formed by the unweighted pair group method with arithmetic averages applied to protein data correlates with the genetic and biochemical information about the species. the results show that protein fingerprinting has the potential to differentiate aeromonas species, but the low qualitative variation indicates that this technique is not efficient for the characterization of strains within a species. conversely, rapd fingerprinting allows the identification of strains but the high variability limits its potential as an aiding method for species identification.
Genetic diversity of cultivated Coffea arabica inbred lines assessed by RAPD, AFLP and SSR marker systems
Maluf, Mirian Perez;Silvestrini, Milene;Ruggiero, Luciana Machado de Campos;Guerreiro Filho, Oliveiro;Colombo, Carlos Augusto;
Scientia Agricola , 2005, DOI: 10.1590/S0103-90162005000400010
Abstract: one of the greatest problems in coffea arabica breeding is identifying precisely any inbred line, based only on botanical and agronomical descriptors, because of the reduced genetic variability of the species, close pedigree origin, which results in small phenotypic variation. recently, molecular markers have been used for plant germplasm characterization and identification in several commercial species. this work evaluates the reliability of three marker systems: rapd, aflp and ssr, to characterize the genetic variability of commercially-used coffea inbred lines developed by the instituto agron?mico (iac), and their potential for cultivar identification. all methods identified polymorphisms among the cultivars. the genetic diversity recognized by the methods is very similar, although is very narrow. rapd and ssr marker systems grouped more efficiently the evaluated cultivars according to parental origin. none of the methods allowed inbred line identification. therefore for varietal protection, it would be necessary using a combination of botanical, agronomical and molecular markers descriptors for precise cultivar identification.
Molecular and Phylogenetic Status of Fasciola sp., of Cattle in Qena, Upper Egypt
Mosaab A. Omar,Asmaa M. Metwally,Khaled Sultan
Pakistan Journal of Biological Sciences , 2013,
Abstract: The species of liver fluke of the genus Fasciola (phylum platyhelminthes, order Digenea, Family Fasciolidae) are obligatory parasites that inhabit the large biliary ducts of herbivore animals as well as man. Reports on the species of Fasciola present in the Nile Delta, Egypt, appear controversial. In the current study a precise identification of Fasciola isolates from cattle in Qena province, Upper Egypt was done based on examination of the second Internal Transcribed Spacer (ITS2) and the mitochondrial cytochrome oxidase subunit I (COI). Amplification, sequencing and phylogenetic examination revealed that the collected Fasciola isolates represent only one species which is Fasciola hepatica.
Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level
Delamare Ana Paula Longaray,Artico Liane de Oliveira,Grazziotin Felipe Gobbi,Echeverrigaray Sergio
Brazilian Journal of Microbiology , 2002,
Abstract: Fifteen well-defined strains of Aeromonas of thirteen species were analyzed by SDS protein electrophoretic analysis (SDS-PAGE) and random amplified polymorphic DNA analysis (RAPD). The comparison between the patterns obtained by both methods allowed differentiating all the strains. Clusters formed by the unweighted pair group method with arithmetic averages applied to protein data correlates with the genetic and biochemical information about the species. The results show that protein fingerprinting has the potential to differentiate Aeromonas species, but the low qualitative variation indicates that this technique is not efficient for the characterization of strains within a species. Conversely, RAPD fingerprinting allows the identification of strains but the high variability limits its potential as an aiding method for species identification.
Análisis morfológico y molecular evidencia problemas al identificar Anopheles nuneztovari (Diptera: Culicidae) por claves dicotómicas
GóMEZ,GIOVAN F; CIENFUEGOS,ASTRID V; GUTIéRREZ,LINA A; CONN,JAN E; CORREA,MARGARITA M;
Revista Colombiana de Entomología , 2010,
Abstract: the oswaldoi group, subgenus nyssorhynchus (diptera: culicidae), includes 16 species. some of these species exhibit high intraspecific morphological variability and interspecific similarity; this can lead to difficulty in the identification of females by using morphological keys, as is the case with anopheles nuneztovari, considered a primary malaria vector in colombia. in this study we compared the usefulness of five morphological keys for the identification of a. nuneztovari specimens collected in the locality of puerto anchica, montelibano, córdoba, colombia. three morphometric ratios were analyzed and morphological identification results were confirmed using the pcr-rflp technique based on its2 sequences. the analysis of 41 females using the keys showed that a. nuneztovari specimens presented overlap with other species of the oswaldoi group, such as a. rangeli, a. oswaldoi, a. evansae and a. benarrochi. molecular analysis confirmed that all of the specimens corresponded to a. nuneztovari. because the use of morphological keys continue to be a strategy of choice for the identification of anophelines, the results suggest that in the case of problematic species, it is convenient to confirm identification using molecular methods developed with the support of the identification of immature stages.
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