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Search Results: 1 - 10 of 8980 matches for " Mohamed Makni "
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Agent Petri Nets Framework for Modeling Staphylococcus epidermidis Biofilm Formation  [PDF]
Borhan Marzougui, Kamel Barkaoui, Mohamed Amine Makni
E-Health Telecommunication Systems and Networks (ETSN) , 2016, DOI: 10.4236/etsn.2016.51003
Abstract: This Staphylococcus epidermidis has been discovered as the most frequent germ detected during indwelling medical devices infection. This fact is well attached with the ability of this bacterium to form structured layered population known as biofilm. Inside S. epidermidis biofilm, bacterial cells present more different behavior than in their planktonic counterpart. This paper describes the thriving application of Petri net theory for modeling of interaction between different regulations actors leading S. epidermidis to switch from Planctonik to Biofilm. Indeed this biologic system is very sensible and has dangerous effect. We propose Agent Petri Nets model to describe and analyze the process of formation of Biofilm molecule. This model presents a formal framework based on Multi Agents system characteristics.
Colocynth (Citrullus colocynthis) Flesh Extract Suppresses Adipogenesis by Down-Regulating Adipogenic Transcription Factors and Their Target Genes in 3T3-L1 Preadipocytes  [PDF]
Raoua Jemai, Riadh Drira, Hamadi Fetoui, Mohamed Makni, Kazuichi Sakamoto
Food and Nutrition Sciences (FNS) , 2018, DOI: 10.4236/fns.2018.98074
Abstract: Citrullus colocynthis, a member of the Cucurbitaceae family, is widely distributed in North Africa. The fruits are recognized for their wide range of medicinal uses and promising pharmaceutical potential. The present study aimed to investigate the anti-obesity effect of the ethanol extract of colocynth flesh (FCEE) in 3T3-L1 cells following treatment at different doses. The viability of 3T3-L1 preadipocytes was analyzed via MTT assay and triglycerides were stained with Oil red O to assess lipid accumulation. Additionally, adipogenesis-related gene expression was quantified via qRT-PCR. FCEE (0 - 150 μg/mL) dose-dependently suppressed intracellular triglyceride accumulation during the adipogenesis by 23% and 66% at 100 and 150 μg/mL, respectively, but did not affect cell viability. Analysis of the time-dependence of the effect of FCEE demonstrated that the greatest anti-adipogenic activity was observed during the early stages of differentiation. FCEE also decreased GPDH activity in a dose-dependent manner, with 98% decrease observed at 150 μg/mL. In addition, at same range of FCEE concentrations, the main transcription factors, including CCAAT/enhancer binding protein α (C/EBPα), peroxisome proliferator activated receptor γ (PPARγ), and sterol regulatory element-binding protein 1c (SREBP-1c), were downregulated by 90%, 89%, and 89%, respectively at 150 μg/mL. As these are the master regulators of adipogenesis. The inhibition of their downstream target genes was also observed. Colocynth may be useful in the treatment of obesity owing to its powerful effects on fat, which result in changes to adipocyte differentiation and fat mobilization.
Hessian Fly, Mayetiola destructor (Say), Populations in the North of Tunisia: Virulence, Yield Loss Assessment and Phenological Data
Makni,Hanem; Bouktila,Dhia; Mezghani,Maha; Makni,Mohamed;
Chilean journal of agricultural research , 2011, DOI: 10.4067/S0718-58392011000300009
Abstract: hessian fly, mayetiola destructor (say), is a destructive pest of wheat worldwide and an endemic pest in tunisia. two natural populations of this insect from the north of tunisia were evaluated, in the field, for their virulence, based on response developed by bread wheat (triticum aestivum l.) cultivars carrying h3, h5, h6, h7h8, h11, h13 and h16 resistance genes. h11, h13 and h16 showed a high effectiveness against both populations; therefore, their implication in hessian fly breeding programs would be of interest. the level of infestation, as well as the yield loss, was assessed, based on the percentage of infested plants and variation in growth parameters due to infestation. the percentage of infested plants, over a 2-yr period in mateur, averaged 18.82% for durum wheat (triticum turgidum l. subsp. durum (desf.) husn.) and 32.50% for bread wheat. for the improved durum wheat cv. karim used as reference, the plant height, number of internodes, number of productive tillers per plant, and 100-seed weight were negatively affected by infestation, while the number of tillers per plant was positively affected. aiming to update information about the annual number of the fly generations occurring on wheat, we surveyed infestation in jédéida. at least three hessian fly generations were detected on bread wheat and durum wheat. continued regular surveying of hessian fly populations in terms of virulence, impact on yield and annual generations is required for optimal deployment of resistance genes and integrated management of hessian fly across all wheat production areas.
Description of the karyotype of Tunisian Mayetiola hordei (Diptera: Cecidomyiidae) and comparison with M. destructor
BOUKTILA,DHIA; MEZGHANI,MAHA; MAKNI,MOHAMED; MAKNI,HANEM;
Revista Colombiana de Entomología , 2009,
Abstract: we report information about the structure of the karyotype of m. hordei, a fly that infests barley in the south of tunisia. the number of chromosomes was found to be (2n = 8) in somatic female cells and (2n – 2 = 6) in somatic male cells, which is similar to the karyotype of m.destructor. mitotic and polytene chromosomes of m. hordei are described and compared with those of m. destructor. the karyological data are discussed in terms of genetic mechanisms underlying divergence of m. hordei and m. destructor.
Description of the karyotype of Tunisian Mayetiola hordei (Diptera: Cecidomyiidae) and comparison with M. destructor Descripción del kariotipo de Mayetiola hordei (Diptera: Cecidomyiidae) de Túnez y comparación con M. destructor
DHIA BOUKTILA,MAHA MEZGHANI,MOHAMED MAKNI,HANEM MAKNI
Revista Colombiana de Entomología , 2009,
Abstract: We report information about the structure of the karyotype of M. hordei, a fly that infests barley in the south of Tunisia. The number of chromosomes was found to be (2n = 8) in somatic female cells and (2n – 2 = 6) in somatic male cells, which is similar to the karyotype of M.destructor. Mitotic and polytene chromosomes of M. hordei are described and compared with those of M. destructor. The karyological data are discussed in terms of genetic mechanisms underlying divergence of M. hordei and M. destructor. Se registra información acerca de la estructura del cariotipo de M. Hordei, una mosca que infesta la cebada en el sur de Túnez. El número de cromosomas fue (2n=8) en células somáticas femeninas y (2n-2=6) en células somáticas masculinas, lo cuál es similar al cariotipo de M. destructor. Se describen y comparen las cromosomas mitóticos y politénicos de M. hordei con los de M. destructor. Los datos cariológicos se discuten en términos de los mecanismos genéticos que explican la divergencia entre M. hordei y M. destructor.
Hessian Fly, Mayetiola destructor (Say), Populations in the North of Tunisia: Virulence, Yield Loss Assessment and Phenological Data Poblaciones de Mosca de Hess, Mayetiola destructor (Say), en el Norte de Túnez: Virulencia, Evaluación de Pérdida de Producción y Datos Fenológicos
Hanem Makni,Dhia Bouktila,Maha Mezghani,Mohamed Makni
Chilean Journal of Agricultural Research , 2011,
Abstract: Hessian fly, Mayetiola destructor (Say), is a destructive pest of wheat worldwide and an endemic pest in Tunisia. Two natural populations of this insect from the North of Tunisia were evaluated, in the field, for their virulence, based on response developed by bread wheat (Triticum aestivum L.) cultivars carrying H3, H5, H6, H7H8, H11, H13 and H16 resistance genes. H11, H13 and H16 showed a high effectiveness against both populations; therefore, their implication in Hessian fly breeding programs would be of interest. The level of infestation, as well as the yield loss, was assessed, based on the percentage of infested plants and variation in growth parameters due to infestation. The percentage of infested plants, over a 2-yr period in Mateur, averaged 18.82% for durum wheat (Triticum turgidum L. subsp. durum (Desf.) Husn.) and 32.50% for bread wheat. For the improved durum wheat cv. Karim used as reference, the plant height, number of internodes, number of productive tillers per plant, and 100-seed weight were negatively affected by infestation, while the number of tillers per plant was positively affected. Aiming to update information about the annual number of the fly generations occurring on wheat, we surveyed infestation in Jédéida. At least three Hessian fly generations were detected on bread wheat and durum wheat. Continued regular surveying of Hessian fly populations in terms of virulence, impact on yield and annual generations is required for optimal deployment of resistance genes and integrated management of Hessian fly across all wheat production areas. La mosca de Hess, Mayetiola destructor (Say), es una plaga mundial destructiva del trigo y endémica en Túnez. Se evaluaron dos poblaciones naturales de este insecto desde el Norte de Túnez, en el campo, por su virulencia, basado en la respuesta desarrollada por cultivares de trigo panadero (Triticum aestivum L.) portando los genes de resistencia H3, H5, H6, H7H8, H11, H13 y H16. H11, H13 and H16 mostraron una alta efectividad contra ambas poblaciones; por lo tanto, su implicancia en programas de producción de mosca de Hess podría ser de interés. Se evaluaron el nivel de infestación y la pérdida de rendimiento, basado en el porcentaje de plantas infestadas y variación en parámetros de crecimiento debidos a infestación. El porcentaje de plantas infestadas, en un período de 2 a os en Mateur, promedió 18,82% para trigo candeal (Triticum turgidum L. subsp. durum (Desf.) Husn.) y 32,50% para trigo panadero. Para el trigo candeal mejorado cv. Karim usado como referencia, altura de planta, número de en
Genetic diversity of Mayetiola destructor and Mayetiola hordei (Diptera: Cecidomyiidae) by inter-simple sequence repeats (ISSRs)
Maha Mezghani Khemakhem, Mohamed Markakchi, Hanem Makni
African Journal of Biotechnology , 2005,
Abstract: Inter-simple sequence repeats (ISSR) polymorphism was used to reveal genetic variability and phylogenetic relationships within and between three haplotypes of Mayetiola species. A set of 14 ISSR primers were screened representing di-, tri, tetra and penta-nucleotide repeats out of which 10 generated scorable bands and three were able to distinguish one of three haplotypes. The consensus tree constructed using binary data from banding patterns generated by ISSR-PCR clustered the two Mayetiola species according to their mitochondrial haplotype. Moreover, genetic diversity estimated by the coefficient of variation indicates a high intra and inter-haplotypes polymorphism. Our results indicate that ISSR can be useful as DNA-based molecular markers for studying genetic diversity and phylogenetic relationships of Mayetiola haplotypes. African Journal of Biotechnology Vol. 4 (7), pp. 601-606, 2005
Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library
Maha Mezghani-Khemakhem,Dhia Bouktila,Nathalie Casse,Houcine Maaroufi,Mohamed Makni,Hanem Makni
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms131114446
Abstract: Using an enriched library method, seven polymorphic microsatellite loci were isolated from the barley stem gall midge, Mayetiola hordei. Polymorphism at loci was surveyed on 57 individual midges collected on barley in Tunisia. Across loci, polymorphism ranged from two to six alleles per locus. The observed heterozygosity varied between 0.070 and 0.877. Based on the number of alleles detected and the associated levels of heterozygosity, we believe that these loci will prove useful for population genetic studies on M. hordei.
Structure génétique des cécidomyies des céréales en Tunisie
Hanem Makni, Moez Sellami, Mohamed Marrakchi, Nicole Pasteur
Genetics Selection Evolution , 2000, DOI: 10.1186/1297-9686-32-6-577
Abstract: (To access the full article, please see PDF)
Acute pancreatitis due to pancreatic hydatid cyst: a case report and review of the literature
Amin Makni, Mohamed Jouini, Montassar Kacem, Zoubeir Safta
World Journal of Emergency Surgery , 2012, DOI: 10.1186/1749-7922-7-7
Abstract: Hydatid disease caused by the larval stage of the Echinococcus parasite is a public health problem in endemic countries, especially in Tunisia. Hydatid disease can involve any organ. The liver is the most common organ involved and, together with the lungs, account for 90% of cases. Other involved sites (less than 10% of cases) are muscles, bones, kidneys, brain, and spleen. Pancreatic hydatid cysts are rare, accounting for less than 1% of cases [1,2]. Isolated involvement of the pancreas is unusual, and acute pancreatitis secondary caused by primary pancreatic hydatid cyst has rarely been reported (less than 2% of cases in endemic areas) [3]. To our knowledge, 8 cases have been reported in the literature [4-11]. We reviewed and summarized the findings from reported cases of hydatid acute pancreatitis as indicated in the English literature, as well as presenting the findings from our case (see Table 1). Only one article was not available [7] and was not included in Table 1.A 38-year-old man was admitted to our clinic with complaints of diffuse abdominal pain, nausea, vomiting for 7 days. The patient did not have any fever or jaundice. Moreover, he did not have any significant medical antecedents. On physical examination, vital signs were normal. Tenderness in the epigastrium was detected while examination of other systems was normal. Laboratory analyses were as follows: white blood cells were 13 000/mmc; hemoglobin was 14 g/dl; platelets were 142 000/mmc; amylase was 2100 U/l (normal value < 105); alanine aminotransferase (ALT) was 300 U/l (normal value < 40); aspartate transaminase (AST) was 120 U/l (normal value < 40); alkaline phosphatase (ALP) was 270 U/l (normal value < 290); gamma-glutamyl transpeptidase (GGT) was 130 U/l (normal value < 49); total bilirubin was 9 mg/l (normal value < 10); direct bilirubin was 3 mg/l (normal value < 8 mg/l); C-reactive protein was 20 mg/l (normal value < 5); and erythrocyte sedimentation rate was 70 mm/h. Serological tests incl
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