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Search Results: 1 - 10 of 182215 matches for " Moacir Franco de;Mariana "
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Fases do desenvolvimento e diferencia??o testicular em cutias (Dasyprocta aguti) criadas em cativeiros
Assis-Neto, Ant?nio Chaves de;Carvalho, Maria Acelina Martins de;Melo, Maria Isabel Vaz de;Miglino, Maria Angélica;Oliveira, Moacir Franco de;Mariana, Arani Nanci Bomfim;
Brazilian Journal of Veterinary Research and Animal Science , 2003, DOI: 10.1590/S1413-95962003000700010
Abstract: the agouti (dasyprocta aguti) is a wild rodent that, in the northeast region of brazil, is a species that is very used by the low-income population as an alternative protein source for human feeding. thirty-one male agouti were used, coming from the universidade federal do piauí (ufpi) - piauí state, and from escola superior de agricultura de mossoró (esam) - rio grande do norte state - brazil. the animals were divided in to age groups, from the birth to fourteen months old. the average diameter average of the nucleus was obtained by measuring 10 nuclei of the studied cellular type, in each testicle that was in the stage i of the seminiferous epithelium cycle. this was performed in animals that did not present an organized epithelium in well-defined periods due to the age, and measurements were made in transversal sections chosen only for the circular contour. the beginning of the asynchronism of the spermatogenic process was observed since the six months of age. the puberty of the agouti dasyprocta aguti was definitely established at nine months of age, because all the cellular types and free spermatozoa in the tubular lumen were present in a large extent of the testicular parenchyma.
Detection of streptococcus mutans and streptococcus sobrinus in dental plaque samples from Brazilian preschool children by polymerase chain reaction
Franco e Franco, Teresa Cristina Costa;Amoroso, Patrícia;Marin, José Moacir;ávila, Fernando Antonio de;
Brazilian Dental Journal , 2007, DOI: 10.1590/S0103-64402007000400011
Abstract: the purposes of this study were to detect s. mutans and s. sobrinus by polymerase chain reaction (pcr) amplification, and to relate their presence to the incidence of dental caries in 42 brazilian preschool children. dental plaque samples were collected from the cervical margin of all erupted teeth of 5-6 years old children with primary dentition, using a sterile explorer. examination of the dmft (decayed, missing, filled teeth) index, performed following the world health organization (who) caries diagnostic criteria, showed a 2.71 score. prevalence of s. mutans and s. sobrinus was respectively, of 85.7% and 14.3%; no dental plaque sample was either positive or negative for both bacterial species. children harboring either s. mutans or s. sobrinus presented the same caries prevalence. pcr showed good discriminative ability for differentiation between these species, and suggested that it is a technique suitable for epidemiological studies on mutans streptococci.
Ana Lisa Paz Souza,Valéria Veras de Paula,Paulo Henrique Cavalcante,Moacir Franco de Oliveira
Ciência Animal Brasileira , 2008,
Abstract: The effect of premedication with acepromazine and xylazine was evaluated in the dissociative anesthesia of collared-pecccaries (Tayassu tajacu). Twenty one animals randomized allocated into three groups of seven individuals. Groups I and II received acepromazine (0.2 mg/Kg) and group III received xylazine (1.0 mg/Kg) by intramuscular route. After 15 minutes, diazepam 0.5 mg/Kg and ketamine (2.5 mg/Kg) were given to group I and diazepam (0.5 mg/Kg) and ketamine (5.0 mg/Kg) to groups II and III, in the same syringe, intravenously (IV). The vital functions as well as the hemogasometry were evaluated. After the anesthesia, the vital functions average were: cardiac frequency (CF) = 133.85 ± 43.31 bpm, respiratory frequency (RF) = 64.57 ± 29.61 rmpm, temperature (T) = 39.82 ± 0.73°C; CF = 124.57 ± 29.43 bpm, RF = 57.28 ± 16.74 rmpm, T = 39.12 ± 0.93°C; and FC = 126.85 ± 34.15 bpm, RF = 95.42 ± 25.45 rmpm, T = 39.67 ± 0.98°C, for groups I, II and III, respectively. There was no significant difference in the hemogasometry. It was concluded that the tranquilization with acepromazine had highest quality. The protocols were safe, and xylazine (1.0 mg/Kg) was insufficient to sedate the animals, but association of acepromazine/ketamine (5.0 mg/Kg)/diazepam produced better results. KEY WORDS: Acepromazine, collared-peccariy, dissociative anesthesia, Tayassu tajacu, xylazine. Avaliou-se o uso da pré-medica o com acepromazina ou xilazina na anestesia dissociativa de catetos (Tayassu Tajacu). Foram utilizados 21 animais, distribuídos em três grupos de sete indivíduos. Os grupos I e II receberam acepromazina na dose de 0,2 mg/Kg, e o grupo III recebeu xilazina na dose de 1,0 mg/Kg, por via intramuscular. Após quinze minutos, o grupo I recebeu diazepam (0,5 mg/Kg) e cetamina (2,5 mg/Kg), e os grupos II e III, diazepam (0,5 mg/Kg) e cetamina (5,0 mg/kg), na mesma seringa, por via intravenosa. Avaliaram-se fun es vitais e hemogasometria. Após a anestesia, as médias das fun es vitais foram: freqüência cardíaca (FC) = 133,85 ± 43,31 bpm, freqüência respiratória ( ) = 64,57 ± 29,61 mrm e temperatura (T) = 39,82 ± 0,73°C; FC = 124,57 ± 29,43 bpm, = 57,28 ± 16,74 mrm, T = 39,12 ± 0,93°C; e FC = 126,85 ± 34,15 bpm, = 95,42 ± 25,45 mrpm, T = 39,67 ± 0,98°C, para os grupos I, II e III respectivamente. N o houve diferen a significativa na hemogasometria. Concluiu-se que a tranqüiliza o com acepromazina mostrou-se de melhor qualidade. Os protocolos apresentaram-se seguros, a xilazina (1,0 mg/kg) insuficiente para a indu o de seda o dos animais, sendo que e a associa o acepro
PIODERMITE PROFUNDA POR Corynebacterium pseudotuberculosis EM CUTIA (Dasyprocta sp.)
Kilder Dantas Filgueira,Moacir Franco de Oliveira,Jael Soares Batista,Francisco Marlon Carneiro Feijó
Ciência Animal Brasileira , 2010,
Abstract: Bacterial skin diseases represent a significant ratio ofrodents’ pathologies. However, in agoutis (Dasyprocta sp.), amiddle-sized rodent with geographic distribution from Central toSouth America, reports on isolated bacterial agents of cutaneousinflammatory processes are not observed. The present studyaimed to describe a case of deep pyoderma by Corynebacteriumpseudotuberculosis in an agouti (Dasyprocta sp.). The agouti(Dasyprocta sp.) presented the description of cutaneous injury andit was submitted to physical examination. After that, antibiotic andanti-inflammatory were prescribed. The animal died when the treatmentwas interrupted, and it was directed to necropsy. Over there,samples for bacterial culture and histopathological examinationwere obtained. The findings of necropsy were: alopecia, erythema,cellulitis, crusts and ulcers, on the ventral side of the head. Thetegument’s cut surface disclosed purulent and caseous secretion. Thebacterial culture isolated the C. pseudotuberculosis. The cutaneoushistopathology diagnosid deep pyoderma. Because of the reducedknowledge about agoutis pyoderma (Dasyprocta sp.), further investigations on the disease’s etiopathogeny are necessary.
Silvia Maria Mendes Ahid,Moacir Franco de Oliveira,Ana Carla Diógenes Suassuna
Ciência Animal Brasileira , 2008,
Abstract: The register the occurrence of the louse Struthiolipeurus rheae parasitizing rheas (Rhea americana) maintained in conditions conservationist and scientific in the Center of Multiplication of Wild Animals of the Rural Federal University of the Semi-Arid in the municipal of Mossoro, State of Rio Grande do Norte. KEY WORDS: Louse, Phthiraptera, Rhea. Registrou-se a ocorrência do piolho mastigador Struthiolipeurus rheae parasitando emas (Rhea americana) mantidas em condi es conservacionista e científicas no Centro de Multiplica o de Animais Silvestres da Universidade Federal Rural do Semi-árido no município de Mossoró, Estado do Rio Grande do Norte. PALAVRAS-CHAVES: Ema, Phthiraptera, piolho.
Yolk Sac Mesenchymal Progenitor Cells from New World Mice (Necromys lasiurus) with Multipotent Differential Potential
Phelipe Oliveira Favaron, Andrea Mess, S?nia Elisabete Will, Paulo César Maiorka, Moacir Franco de Oliveira, Maria Angelica Miglino
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0095575
Abstract: Fetal membranes are abundant, ethically acceptable and readily accessible sources of stem cells. In particular, the yolk sac is a source of cell lineages that do not express MHCs and are mainly free from immunological incompatibles when transferred to a recipient. Although data are available especially for hematopoietic stem cells in mice and human, whereas other cell types and species are dramatically underrepresented. Here we studied the nature and differentiation potential of yolk sac derived mesenchymal stem cells from a New World mouse, Necromys lasiurus. Explants from mid-gestation were cultured in DMEM-High glucose medium with 10% defined fetal bovine serum. The cells were characterized by standard methods including immunophenotyping by fluorescence and flow cytometry, growth and differentiation potential and tumorigenicity assays. The first adherent cells were observed after 7 days of cell culture and included small, elongated fibroblast-like cells (92.13%) and large, round epithelial-like cells with centrally located nuclei (6.5%). Only the fibroblast-like cells survived the first passages. They were positive to markers for mesenchymal stem cells (Stro-1, CD90, CD105, CD73) and pluripotency (Oct3/4, Nanog) as well as precursors of hematopoietic stem cells (CD117). In differentiation assays, they were classified as a multipotent lineage, because they differentiated into osteogenic, adipogenic, and chondrogenic lineages and, finally, they did not develop tumors. In conclusion, mesenchymal progenitor cells with multipotent differentiation potential and sufficient growth and proliferation abilities were able to be obtained from Necromys yolk sacs, therefore, we inferred that these cells may be promising for a wide range of applications in regenerative medicine.
Análise histométrica do desenvolvimento testicular de cutias (Dasyprocta aguti) criadas em cativeiros
Assis-Neto, Ant?nio Chaves de;Melo, Maria Isabel Vaz de;Carvalho, Maria Acelina Martins de;Miglino, Maria Angélica;Oliveira, Moacir Franco de;
Brazilian Journal of Veterinary Research and Animal Science , 2003, DOI: 10.1590/S1413-95962003000300007
Abstract: the testicular development in 31 agoutis, of the dasyprocta aguti species, from the birth up to 14 months of age was studied through the histometric analysis. mid diameter and the mean area, in each testicle, were obtained through 30 crosscuts of the funiculus and/or the seminiferous tubule, by means of a computerized system for image analysis and a zeiss cpl 10x ocular micrometric device, coupled to an objective lens of 40x. testicles volume proportions were obtained through the stereometric method, according to elias, henning and schwartz1. tubule medium diameter presented a slow growth from the birth to the eight months of age, for both used methodologies. when the ocular micrometric device was used it was observed that, beginning in the ninth month, the tubule diameter had an accelerated growth getting to duplicate its value if compared with the preceded age group. the volume proportion of the testicle cord and the seminiferous tubule grew gradually, reaching its maximum value (86,50%) on the ninth month. the leydig cells presented a decreasing volume proportion, and their largest values were expressive from the birth up to four months of age (7,00 ± 1,77% to 9,55 ± 0,64%) and a minimum value, starting from nine months, still tending to stabilize. the stroma decreased with the development of age and fall abruptly when the puberty starts. it is concluded that the diameter of the testicles cords and seminiferous tubule presented a larger growth coinciding with the start of puberty and the volume proportion of the leydig cells was lower in the same period.
Morfologia e topografia do fígado e pancreas de emas Rhea americana
Rodrigues, Marcio Nogueira;Oliviera, Gleidson Benevides de;Silva, Roberto Sávio Bessa da;Albuquerque, José Fernando Gomes de;Miglino, Maria Angelica;Oliveira, Moacir Franco de;
Ciência Rural , 2012, DOI: 10.1590/S0103-84782012000300014
Abstract: the rhea are ratites birds native of the south american continent, which are considered primitive birds from the standpoint of the phylogenetic group that is highly specialized .this study aimed to characterize gross and microscopically the liver and rhea pancreas. the material was collected in a wild animals multiplication center, mossoró-rn, brazil, (registration n° 14.78912 ibama). it was used 20 young animals aged between two and six months independent of sex. in the rhea the liver is related to the cranial apex of the heart, dorsally with the lungs, esophagus and gastric proventriculus, caudally with the gastric ventricle, the spleen, the duodenum and part of the jejunum. it was dark red color and had only two lobes, being the right lobe slightly smaller than the left. histologically was lined by a thin connective tissue capsule and each lobe liver could be clearly identified by the presence of central veins, with sinusoids communicating with the veins. the pancreas, ventrally, showed a thin tape formed by a dorsal lobe and a ventral lobe. longitudinally the pancreas in rhea is located in the dorsal mesentery since the liver until the cranial duodenal flexure, remaining connected the duodenal handles by ligaments. histologically was composed of a thin capsule of dense connective tissue, with discrete lobules separated by capsular connective tissue, composed of tubuloalveolares structures and pipelines. the liver and pancreas of rhea have morphology similar to that described for domestic poultry.
Alicia Greyce Turatti Pessolato,Mariana Junqueira Franco,Mara Villas Boas de Carvalho
Revista da Universidade Vale do Rio Verde , 2011,
Abstract: The grieving process of family and child/adolescent who experiences such as being the very finitude generally triggers are diagnosed with a degenerative disease with low expectations of cure. The grieving continues sublimated throughout treatment and at the time this change of perspective, grief can be triggered with even more intensity. However, the way you deal with this process may differundergone a process called melancholy, or a natural grieving rocess. Both have a feeling justified by the overall correlation in which they appear, because they have the same external influences that trigger feelings of both. Although the children for their cognitive development and intellectual understands the death just after 6 years of age, it is known that children with cancer come to an understanding of the meaning of death much earlier still not even being the same as adult. Teenagers, in turn, may be more prone to existential crises in a terminal phase, as they associate other types of loss inherent in the very result of the treatment phase and the loss of their identity. In this context, we highlight the importance of having a multidisciplinary team of palliative care and pain control, aimed at holistically address all aspects that involve human beings in order to provide them a dignified dying process and respected by the last moment of their lives.
Flavonóides e triterpenos de Baccharis pseudotenuifolia: bioatividade sobre Artemia salina
Moreira, Francisco de Paula Madeira;Coutinho, Valmor;Montanher, Ana Beatriz Pimentel;Caro, Miguel Soriano Balparda;Brighente, Inês Maria Costa;Pizzolatti, Moacir Geraldo;Monache, Franco Delle;
Química Nova , 2003, DOI: 10.1590/S0100-40422003000300004
Abstract: the phytochemical investigation of the chloroformic and methanolic extracts of the baccharis pseudotenuifolia led to the isolation of triterpenes, steroids and flavonoids. from chloroformic extract were isolated oleanolic acid and a-spinasterol while from methanolic extract were isolated the flavonoids: hispidulin, naringenin, 3'-methoxy-luteolin, apigenin, kaempferol, eriodyctiol, aromadendrin, quercetin, 3'-methoxy-quercetin, quercetin-3-o-rhamnoside and quercetin-3-o-glucoside. the structure of these compounds were identified by ir, cg/ms, 1h and 13c nmr spectral analysis and comparison with literature. in addition, the extracts were evaluated by means of brine shrimp lethality test and the highier activity was observed in the chloroformic extract.
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