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Search Results: 1 - 10 of 1563 matches for " Mitsuhiro Ueda "
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Distribution of Chitinolytic Enzyme in the Organs and Molecular Cloning of a Novel Chitinase Gene from the Kidney of Marbled Rockfish Sebastiscus marmoratus  [PDF]
Miku Watanabe, Hiromi Kakizaki, Taro Tsukamoto, Miku Fujiwara, Hideto Fukushima, Mitsuhiro Ueda, Masahiro Matsumiya
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.91004
Abstract: Actinopterygii express two types of chitinase (acidic fish chitinase-1 (AFCase-1) and acidic fish chitinase-2 (AFCase-2)) that are active at acidic pHs and involved in digestion in the stomach. We proposed the existence of a new fish chitinase that has a non-digestive function. In this study, we used Sebastiscus marmoratus, for which characteristics and cDNA cloning of chitinase isozymes (SmChi-1, SmChi-2) in the stomach have been reported. Initially, we examined the distribution of chitinase and β-N-acetylhexosaminidase (Hex) in the body and then we tried to clone novel chitinase cDNA from the kidney. Chitinase and Hex activities were measured using pNP-(GlcNAc)n, (n = 2, 3) and pNP-GlcNAc as substrates, respectively. Total RNA was extracted from the kidney. RT-PCR was performed to obtain chitinase cDNA fragments using reverse transcriptase with an oligo dT primer. The RACE method was used to obtain sequences of the upstream and downstream regions of cDNA. The full-length chitinase cDNA was determined using PrimeSTAR®?Max DNA polymerase with proofreading activity. High chitinase activity was observed
Chondrichthyes Chitinase: Molecular Cloning, Distribution, and Phylogenetic Analysis  [PDF]
Miku Watanabe, Hiromi Kakizaki, Momo Kanai, Satoshi Kawashima, Kaneyuki Hamaguchi, Hiroki Mizuno, Tsubasa Ueno, Chiaki Yasukawa, Ryuji Agata, Mana Ikeda, Hideto Fukushima, Mitsuhiro Ueda, Masahiro Matsumiya
Open Journal of Marine Science (OJMS) , 2017, DOI: 10.4236/ojms.2018.81007
Abstract:
We have previously reported the presence of three types of chitinase (acidic fish chitinase-1: AFCase-1, acidic fish chitinase-2: AFCase-2, fish chitinase-3: FCase-3) in Actinopterygii. In the present research, we report the identification of the novel chitinase genes HjChi (ORF: 1380 bp) and DkChi (ORF: 1440 bp) from the stomach of Chondrichthyes, Japanese bullhead shark (Heterodontus japonicas) and Kwangtung skate (Dipturus kwangtungensis), respectively. Organ-specific expression analysis identified the stomach-specific expression of HjChi, whereas DkChi was expressed widely in all organs. Chitinase activity was measured using pNP-(GlcNAc)n (n = 2, 3) as a substrate and β-N-acetylhexosaminidase (Hex) activity was measured using pNPGlcNAc. Relatively high values of chitinase activity were observed in the stomach, spleen, and gonads of the Japanese bullhead shark, H. japonicas , compared with that observed in the stomach of the Kwangtung skate D. kwangtungensis . However, Hex activity was detected throughout the body of both species. The optimal pH of chitinase in both the Japanese bullhead shark, H. japonicas, and the Kwangtung skate, D. kwangtungensis, were 3.5 - 5.5 and 3.5 - 4.0, respectively, and 4.0 for Hex in both species. Phylogenetic analysis revealed that Chondrichthyes chitinase forms a unique group (Chondrichthyes chitinase). These results suggested that the possibility of the formation of chitinase groups for each class in the phylogenetic analysis based on the observation of class-specific chitinase.
Derivative of a Determinant with Respect to an Eigenvalue in the LDU Decomposition of a Non-Symmetric Matrix  [PDF]
Mitsuhiro Kashiwagi
Applied Mathematics (AM) , 2013, DOI: 10.4236/am.2013.43069
Abstract:

We demonstrate that, when computing the LDU decomposition (a typical example of a direct solution method), it is possible to obtain the derivative of a determinant with respect to an eigenvalue of a non-symmetric matrix. Our proposed method augments an LDU decomposition program with an additional routine to obtain a program for easily evaluating the derivative of a determinant with respect to an eigenvalue. The proposed method follows simply from the process of solving simultaneous linear equations and is particularly effective for band matrices, for which memory requirements are significantly reduced compared to those for dense matrices. We discuss the theory underlying our proposed method and present detailed algorithms for implementing it.

Derivative of a Determinant with Respect to an Eigenvalue in the Modified Cholesky Decomposition of a Symmetric Matrix, with Applications to Nonlinear Analysis  [PDF]
Mitsuhiro Kashiwagi
American Journal of Computational Mathematics (AJCM) , 2014, DOI: 10.4236/ajcm.2014.42009
Abstract:

In this paper, we obtain a formula for the derivative of a determinant with respect to an eigenvalue in the modified Cholesky decomposition of a symmetric matrix, a characteristic example of a direct solution method in computational linear algebra. We apply our proposed formula to a technique used in nonlinear finite-element methods and discuss methods for determining singular points, such as bifurcation points and limit points. In our proposed method, the increment in arc length (or other relevant quantities) may be determined automatically, allowing a reduction in the number of basic parameters. The method is particularly effective for banded matrices, which allow a significant reduction in memory requirements as compared to dense matrices. We discuss the theoretical foundations of our proposed method, present algorithms and programs that implement it, and conduct numerical experiments to investigate its effectiveness.

Surgical Treatment by Partial Petrosectomy for a Middle-Ear Carcinoid with Progressive Extension: A Case Report and Review of the Literature
Mitsuhiro Aoki,Keisuke Mizuta,Natsuko Ueda,Nansei Yamada,Yatsuji Ito,Hiroki Kato,Yoshinobu Hirose
International Journal of Otolaryngology , 2010, DOI: 10.1155/2010/818673
Abstract: We herein report a 59-year-old male patient with a recurrent carcinoid tumor of the middle ear 7 years after a tympanomastoidectomy. The CT and dynamic MRI demonstrated an extensive tumor close to the carotid artery canal and the jugular bulb, and the tumor was removed by a partial petrosectomy with a transmastoid approach. The histopathological findings revealed a solid and trabecular tumor with cells positive for cytokeratin, chromogranin A, synaptophysin, and CD56. The MIB-1 antibody for the Ki-67 antigen was positive in 6.6% of the tumor cells. The relevant literature is reviewed in regard to the present case.
Surgical Treatment by Partial Petrosectomy for a Middle-Ear Carcinoid with Progressive Extension: A Case Report and Review of the Literature
Mitsuhiro Aoki,Keisuke Mizuta,Natsuko Ueda,Nansei Yamada,Yatsuji Ito,Hiroki Kato,Yoshinobu Hirose
International Journal of Otolaryngology , 2010, DOI: 10.1155/2010/818673
Abstract: We herein report a 59-year-old male patient with a recurrent carcinoid tumor of the middle ear 7 years after a tympanomastoidectomy. The CT and dynamic MRI demonstrated an extensive tumor close to the carotid artery canal and the jugular bulb, and the tumor was removed by a partial petrosectomy with a transmastoid approach. The histopathological findings revealed a solid and trabecular tumor with cells positive for cytokeratin, chromogranin A, synaptophysin, and CD56. The MIB-1 antibody for the Ki-67 antigen was positive in 6.6% of the tumor cells. The relevant literature is reviewed in regard to the present case. 1. Introduction Carcinoid tumors primarily originate from gastrointestinal organs and the trachea. Carcinoid tumors of the middle ear are very rare; however, there have been more than 50 reports of such tumors since 1980 [1]. A middle-ear carcinoid tumor is usually confined to the tympanum, and osteolytic extension of the tumor is rare [2]. Several patients show osteolytic invasion and cervical lymph node metastasis, suggesting that the middle-ear carcinoid should be classified as a low-grade malignancy [3–5]. The current report presents a patient with extensive osteolytic enlargement of a middle-ear carcinoid close to the jugular bulb and carotid artery canal, and also reviews the previous studies of carcinoid tumors of the middle ear. 2. Case Report A 59-year-old male patient presented with ear pain and bleeding of the left ear, and upon closer investigation a reddish bulging mass extending through the left tympanic membrane from the middle ear was observed. The pure tone audiogram showed an 80-dB mixed hearing loss with an increased threshold of bone conduction in the high tone frequency range. The patient experienced no dizziness or facial palsy. The tympanum and mastoid were filled with an isodensity shadow indicating bone erosion, and the wall of the carotid artery canal and the jugular bulb appeared to be thick and erosive on CT (Figure 1). The mass was close to the carotid artery and jugular bulb through the tympanum, and the mastoid space was enhanced in the early and late phases of the dynamic MRI. The enhanced mass also appeared on the underside of the promontory of the middle ear (Figure 2). The patient had experienced a tympanomastoidectomy for tumors in the tympanum 7 years previously and the pathological diagnosis was adenoma of the middle ear. Figure 1: CT. The tympanum and mastoid were filled with an isodensity shadow with bone erosion. The wall of the carotid artery and jugular bulb appeared to be thick and erosive. CA:
Chromosomal Studies of Masculinized Hybrids in Bitterlings (Teleostei: Cypriniformes: Acheilognathinae)  [PDF]
Takayoshi Ueda, Yukie Ueda
Natural Resources (NR) , 2016, DOI: 10.4236/nr.2016.76028
Abstract: The chromosome analysis of the masculinized hybrid between female Tanakia limbata and male T. signifer in bitterlings (Acheilognathinae) was done. It was presumed that they had intermediate karyotype between the parents, and formed sperms with heteroploidy resulting from the incomplete pairing of homologous chromosomes in meiosis. Due to the abundance of species and the ease of artificial fertilization, the study of the factor of the hybrid sterility in bitterlings would lead to the clarification of the mechanism about species differentiation and karyotype differentiation, and also to developing a new variety.
Chromosomal Studies of the Hybrid between Female Rhodeus ocellatus ocellatus and Male Rhodeus atremius fangi in Bitterlings (Teleostei: Cypriniformes: Acheilognathinae)  [PDF]
Takayoshi Ueda, Yukie Ueda
Natural Resources (NR) , 2018, DOI: 10.4236/nr.2018.91002
Abstract:
The chromosome analysis of the masculinized hybrid between female Rhodeus ocellatus ocellatus and male R. atremius fangi in bitterlings (Acheilognathinae) was done. It was presumed that they had intermediate karyotype between the parents, and formed sperms with heteroploidy resulting from the incomplete pairing of homologous chromosomes in meiosis. Due to the abundance of species and the ease of artificial fertilization, the study of the factor of the hybrid sterility in bitterlings would lead to the clarification of the mechanism about species differentiation and karyotype differentiation, and also develop a new variety. And also, it would also be important to make the hybrid various natures clear in environmental preservation.
Increased serum HO-1 in hemophagocytic syndrome and adult-onset Still's disease: use in the differential diagnosis of hyperferritinemia
Yohei Kirino, Mitsuhiro Takeno, Mika Iwasaki, Atsuhisa Ueda, Shigeru Ohno, Akira Shirai, Heiwa Kanamori, Katsuaki Tanaka, Yoshiaki Ishigatsubo
Arthritis Research & Therapy , 2005, DOI: 10.1186/ar1721
Abstract: Heme oxygenase (HO) is an enzyme that catalyzes the conversion of heme into CO, Fe2+, and biliverdin [1,2]. HO-1, an inducible form of HO, is a 32-kD heat shock protein expressed in response to various noxious stimuli including heavy metals, hyperoxia, hypoxia, endotoxin, hydrogen peroxide, and inflammatory cytokines [1,2]. Evidence suggests that increased expression of HO-1 can benefit the host in a variety of pathological conditions [1-5]. In this context, our research team has found that HO-1 gene therapy is useful for lipopolysaccharide-induced lung injury [6], influenza viral pneumonia [7], bleomycin-induced pulmonary fibrosis [8], and chronic respiratory infection with Pseudomonas aeruginosa in mice [9]. We also found that chemically induced HO-1 was of benefit in lupus nephritis [10]. On the other hand, a deficiency in HO-1 expression is associated with severe chronic inflammation, as shown in studies of HO-1 knockout mice (mice in which the gene for HO-1 had been inactivated) and a patient with HO-1 deficiency [11-13]. This observation is consistent with HO-1 having a physiological effect in protecting against inflammation.Products of heme degradation mediate the protective effects of HO-1. CO suppresses apoptosis, macrophage activation, and the synthesis of proinflammatory cytokines, nitrite oxide, and prostaglandins [1,2,14]. Biliverdin is converted into bilirubin, an antioxidant [1,2,15-18]. Fe2+, which itself has toxic effects by inducing the formation of free radicals, stimulates the production of ferritin [19]. Ferritin acts as an antioxidant and detoxifies Fe2+ [19]. Thus, the heme degradation products and the metabolic derivatives generated by HO-1 suppress toxic events in cells.Regulation of HO-1 is of particular interest in the inflammation associated with hyperferritinemia, as is the case in hemophagocytic syndrome (HPS) and adult-onset Still's disease (ASD), because HO-1 can be involved in increased ferritin in these conditions [1,2]. HPS is a se
A New Analysis Concept in Applying Software Reliability Growth Models and Tool Implementation: The SafeMan  [PDF]
Takaji Fujiwara, Mitsuhiro Kimura
Journal of Software Engineering and Applications (JSEA) , 2014, DOI: 10.4236/jsea.2014.75036
Abstract:

In recent years, many software development organizations have been assessing and analyzing their software product’s reliability/quality and judging whether the software product is releasable by using Software Reliability Growth Models (SRGMs) at the final stage of software development. The usage of SRGMs originates in the advantage that various reliability analysis results based on the SRGMs can be acquired easily. However, it is very difficult for general software project managers to grasp the achievement level of reliability/quality based on its analysis results because some sort of professional knowledge is required in order to understand the information on the attainment progress of software product’s reliability/quality. Moreover, it is also difficult for software project managers and inspectors who do not deeply comprehend the details of their project to evaluate the degree of software reliability and quality, if they assess it without grasping the live development situation and only see the documents submitted from their staff. In this paper, we propose a new analysis concept for assessing the software product’s reliability/quality, and illustrate the output results obtained by a tool, the SafeMan.

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