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Search Results: 1 - 10 of 5192 matches for " Mirian Perez;Eira "
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Caracteriza??o de cultivares de Coffea arabica mediante utiliza??o de descritores mínimos
Aguiar, Adriano Tosoni da Eira;Guerreiro-Filho, Oliveiro;Maluf, Mirian Perez;Gallo, Paulo Boller;Fazuoli, Luiz Carlos;
Bragantia , 2004, DOI: 10.1590/S0006-87052004000200003
Abstract: the species coffea arabica is responsible for 70% of the world coffee production. the majority of cultivars has a narrow genetic origin, being derived basically from two old cultivars: tipica and bourbon. this investigation was carried out aiming at the characterization of selected cultivars of the instituto agronomico according to standard descriptors. thirty five morphological or technological traits of canopy, leaves, flowers, fruits or seeds and three agronomic traits were evaluated in twenty nine cultivar lines of the current breeding program. the variables plant height, fruit color, leaf rust resistance and earliness are sufficient for an efficient identification of groups of cultivars. the color of young leaves and plant diameter are important descriptors for discriminating cultivars of the mundo novo group, but they could not identify cultivars of the catuaí vermelho, catuaí amarelo and icatu vermelho groups.
Cell Stress by Phosphate of Two Protozoa Tetrahymena thermophile and Tetrahymena pyriformis  [PDF]
Papa Daouda Mar, Bouchra El Khalfi, Jose Roman Perez-Casti?eira, Aurelio Serrano, Abdelaziz Soukri
Advances in Bioscience and Biotechnology (ABB) , 2017, DOI: 10.4236/abb.2017.812033
Abstract: Phosphorus is one of the bioelements most needed as a compound cell by living organisms. Phosphorus is involved in several pathologies: in human with bone and kidney diseases, in mammals with metabolism disorder (glucose, insulin···), in microorganisms whose phosphorus is involved in cell growth. Phosphorus has various forms including pyrophosphate, a by-product of multiple pathways of biosynthesis. Enzymes that hydrolyze pyrophosphate are called inorganic pyrophosphatases (PPases). Two major types of inorganic pyrophosphatases are distinguished: the soluble pyrophosphatases (sPPases) and the membrane pyrophosphatases (mPPases or H+/Na+-PPases). They play a key role in the control of intracellular inorganic pyrophosphate level and produce an important ions gradient (H+ or Na+) to the cells. In this work, we primarily focused on the physiological study in a phosphate-poor medium of two models Tetrahymena thermophile and Tetrahymena pyriformis, following the mobility, the growth and the morphology of cells. Secondly, we evaluated the enzymatic activity of soluble and membrane pyrophosphatases in both species grown in the same complex medium. A decrease of cell growth is correlated with unusual morphologies and different mobility in the stress medium. The measurement of soluble and membrane inorganic pyrophosphatases activities also shows a decrease which illustrates the lack of phosphate found in the stress medium. Deficiency of phosphate is a limiting factor for protozoan growth. These results indicate that Tetrahymena can be used as
Inorganic Pyrophosphatases: Study of Interest  [PDF]
Mar Papa Daouda, El Khalfi Bouchra, Perez-Casti?eira Jose Roman, Serrano Delgado Aurelio, Soukri Abdelaziz
Advances in Bioscience and Biotechnology (ABB) , 2017, DOI: 10.4236/abb.2017.810028
Abstract: Inorganic pyrophosphatases are enzymes that catalyze the hydrolysis of inorganic pyrophosphate to orthophosphate. These enzymes are divided into two groups: the soluble pyrophosphatases and the membrane pyrophosphatases. They vary in structure and each has a determined catalysis mechanism. Soluble pyrophosphatases are ubiquitous enzymes and play a key role in regulating the rate of pyrophosphate and balance in this sense, the biosynthetic reactions. Membrane pyrophosphatases are ion pumps, producing a proton or sodium gradient, and provide critical energy reserves to organisms, especially during stress conditions. Several studies have shown that these enzymes are involved in numerous disorders (diseases, fault cell growth···). However they are potential targets for the development of agents against parasites. This article consists of a description of the different types, structures, catalytic properties of inorganic pyrophosphatases and their involvement in cellular metabolism.
Coffee seed physiology
Eira, Mirian T. S.;Silva, E. A. Amaral da;De Castro, Renato D.;Dussert, Stéphane;Walters, Christina;Bewley, J. Derek;Hilhorst, Henk W. M.;
Brazilian Journal of Plant Physiology , 2006, DOI: 10.1590/S1677-04202006000100011
Abstract: considerable advances in our understanding of coffee seed physiology have been made in recent years. however, despite intense research efforts, there are many aspects that remain unclear. this paper gives an overview of the current understanding of the more important features concerning coffee seed physiology, and provides information on recent findings on seed development, germination, storage and longevity.
Brazilian coffee genome project: an EST-based genomic resource
Vieira, Luiz Gonzaga Esteves;Andrade, Alan Carvalho;Colombo, Carlos Augusto;Moraes, Ana Heloneida de Araújo;Metha, ?ngela;Oliveira, Angélica Carvalho de;Labate, Carlos Alberto;Marino, Celso Luis;Monteiro-Vitorello, Claúdia de Barros;Monte, Damares de Castro;Giglioti, éder;Kimura, Edna Teruko;Romano, Eduardo;Kuramae, Eiko Eurya;Lemos, Eliana Gertrudes Macedo;Almeida, Elionor Rita Pereira de;Jorge, érika C.;Albuquerque, érika V. S.;Silva, Felipe Rodrigues da;Vinecky, Felipe;Sawazaki, Haiko Enok;Dorry, Hamza Fahmi A.;Carrer, Helaine;Abreu, Ilka Nacif;Batista, Jo?o A. N.;Teixeira, Jo?o Batista;Kitajima, Jo?o Paulo;Xavier, Karem Guimar?es;Lima, Liziane Maria de;Camargo, Luis Eduardo Aranha de;Pereira, Luiz Filipe Protasio;Coutinho, Luiz Lehmann;Lemos, Manoel Victor Franco;Romano, Marcelo Ribeiro;Machado, Marcos Antonio;Costa, Marcos Mota do Carmo;Sá, Maria Fátima Grossi de;Goldman, Maria Helena S.;Ferro, Maria Inês T.;Tinoco, Maria Laine Penha;Oliveira, Mariana C.;Van Sluys, Marie-Anne;Shimizu, Milton Massao;Maluf, Mirian Perez;Eira, Mirian Therezinha Souza da;Guerreiro Filho, Oliveiro;Arruda, Paulo;Mazzafera, Paulo;Mariani, Pilar Drummond Sampaio Correa;Oliveira, Regina L.B.C. de;Harakava, Ricardo;Balbao, Silvia Filippi;Tsai, Siu Mui;Mauro, Sonia Marli Zingaretti di;Santos, Suzana Neiva;Siqueira, Walter José;Costa, Gustavo Gilson Lacerda;Formighieri, Eduardo Fernandes;Carazzolle, Marcelo Falsarella;Pereira, Gon?alo Amarante Guimar?es;
Brazilian Journal of Plant Physiology , 2006, DOI: 10.1590/S1677-04202006000100008
Abstract: coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. the genus coffea belongs to the rubiaceae family which includes other important plants. the genus contains about 100 species but commercial production is based only on two species, coffea arabica and coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. the brazilian coffee genome project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. we have single-pass sequenced a total of 214,964 randomly picked clones from 37 cdna libraries of c. arabica, c. canephora and c. racemosa, representing specific stages of cells and plant development that after trimming resulted in 130,792, 12,381 and 10,566 sequences for each species, respectively. the ests clustered into 17,982 clusters and 32,155 singletons. blast analysis of these sequences revealed that 22 % had no significant matches to sequences in the national center for biotechnology information database (of known or unknown function). the generated coffee est database resulted in the identification of close to 33,000 different unigenes. annotated sequencing results have been stored in an online database at http://www.lge.ibi.unicamp.br/cafe. resources developed in this project provide genetic and genomic tools that may hold the key to the sustainability, competitiveness and future viability of the coffee industry in local and international markets.
Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance
Pinto, Fernanda de Oliveira;Maluf, Mirian Perez;Guerreiro-Filho, Oliveiro;
Pesquisa Agropecuária Brasileira , 2007, DOI: 10.1590/S0100-204X2007000300011
Abstract: the objective of this work was to identify expressed simple sequence repeats (ssr) markers associated to leaf miner resistance in coffee progenies. identification of ssr markers was accomplished by directed searches on the brazilian coffee expressed sequence tags (est) database. sequence analysis of 32 selected ssr loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. also, expressed ssr are localized frequently in the 5'-utr of gene transcript. moreover, most of the genes containing ssr are associated with defense mechanisms. polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a coffea arabica x coffea racemosa hybrid. frequency of ssr alleles was 2.1 per locus. however, no polymorphism associated with leaf miner resistance was identified. these results suggest that marker-assisted selection in coffee breeding should be performed on the initial cross, in which genetic variability is still significant.
Genetic diversity of cultivated Coffea arabica inbred lines assessed by RAPD, AFLP and SSR marker systems
Maluf, Mirian Perez;Silvestrini, Milene;Ruggiero, Luciana Machado de Campos;Guerreiro Filho, Oliveiro;Colombo, Carlos Augusto;
Scientia Agricola , 2005, DOI: 10.1590/S0103-90162005000400010
Abstract: one of the greatest problems in coffea arabica breeding is identifying precisely any inbred line, based only on botanical and agronomical descriptors, because of the reduced genetic variability of the species, close pedigree origin, which results in small phenotypic variation. recently, molecular markers have been used for plant germplasm characterization and identification in several commercial species. this work evaluates the reliability of three marker systems: rapd, aflp and ssr, to characterize the genetic variability of commercially-used coffea inbred lines developed by the instituto agron?mico (iac), and their potential for cultivar identification. all methods identified polymorphisms among the cultivars. the genetic diversity recognized by the methods is very similar, although is very narrow. rapd and ssr marker systems grouped more efficiently the evaluated cultivars according to parental origin. none of the methods allowed inbred line identification. therefore for varietal protection, it would be necessary using a combination of botanical, agronomical and molecular markers descriptors for precise cultivar identification.
Consumo de alimentos de risco e prote??o para doen?as cardiovasculares entre funcionários públicos
Neumann, Africa Isabel de la Cruz Perez;Shirassu, Mirian Matsura;Fisberg, Regina Mara;
Revista de Nutri??o , 2006, DOI: 10.1590/S1415-52732006000100002
Abstract: objective: the aim of this study is to describe the consumption of protective and promotive foods in cardiovascular diseases among public employees according to level of education and family income. methods: an epidemiologic investigation was carried out with public employees of state offices in s?o paulo city, both sexes and aged up 18th on utilizing random sampling weighted (n=1 271) select from a total of 4 665 persons. information about socioeconomic characteristics, lifestyle and the food consumption, as well as referred to morbidity was obtained. food frequency questionnaire was used to collect information about food habits. results: risk factors such as sedentary lifestyle (88%), overweight/obesity (36%), smoking status (27%) and cardiovascular diseases (30%) were verified. the mean consumption of foods that promote cardiovascular diseases, that is, saturated fatty acids, sodium and sugars, was statistically higher among individuals with a low level of education (elementary school) and a family income of up to 3 minimum wages. the mean consumption of protective foods, such as dietary fiber, vitamins, minerals, polyunsaturated and monounsaturated fatty acids and phytochemicals was statistically higher among those with higher education (college degree) and a family income of more than 6 minimum wages. conclusion: individuals with a low level of education (elementary school) and a family income of up to 3 minimum wages consume more foods that promote cardiovascular diseases. they also presented other associated risk factors more frequently. intervention programs must prioritize this segment of the population.
Paleoamericans in Northeast Argentina  [PDF]
Daniel Loponte, Mirian Carbonera
Archaeological Discovery (AD) , 2017, DOI: 10.4236/ad.2017.52005
Abstract: In this paper, we present a brief synthesis of the record of Fell or Fishtail points found in northeast Argentina, including the first two records for Misiones province. To date, 11 pieces have been identified in the region, which demonstrate techno-morphological similarities with specimens recovered in different areas of South America, which are linked to Paleoamerican groups of the Pleistocene-Holocene boundary. Although these points are still scarce in this area, this is probably due to the lack of research and issues related to archaeological visibility. These new records increase the density of findings of these early hunter-gatherers on the South American Atlantic slope.
Atributos fenológicos e agron?micos em cultivares de cafeeiro arábica
Gaspari-Pezzopane, Cristiana de;Favarin, José Laércio;Maluf, Mirian Perez;Pezzopane, José Ricardo Macedo;Guerreiro Filho, Oliveiro;
Ciência Rural , 2009, DOI: 10.1590/S0103-84782009005000007
Abstract: the aim of this study was to evaluate cultivars of coffea arabica on the basic of phenology and agronomic attributes a function of the year of production. the phenological attributes were evaluated regarding phenology and percentage of cherry fruits at harvesting time. agronomic traits evaluated included productivity and outturn, type of seeds and grain size. the period between the blooms till the fruits at the beginning of growth did not vary among the coffee trees in the year of production 2004/2005. while in the following year the icatu precoce cultivar presented anticipation of the fruits at the beginning of growth and the 'obat?' cultivar delayed the beginning of the phenological cycle due to the other cultivars studied. the duration of each cycle varied with the year of production and then all the coffee trees in this study could be differentiated on the timing of maturation (early, middle and late). the agronomic attributes (productivity, outturn, type and size of grains), regardless the different cultivars of coffee also varied with the year of production.
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