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Search Results: 1 - 10 of 38138 matches for " Ming-Long Yeh "
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The Influence of Physical and Physiological Cues on Atomic Force Microscopy-Based Cell Stiffness Assessment
Yu-Wei Chiou, Hsiu-Kuan Lin, Ming-Jer Tang, Hsi-Hui Lin, Ming-Long Yeh
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0077384
Abstract: Atomic force microscopy provides a novel technique for differentiating the mechanical properties of various cell types. Cell elasticity is abundantly used to represent the structural strength of cells in different conditions. In this study, we are interested in whether physical or physiological cues affect cell elasticity in Atomic force microscopy (AFM)-based assessments. The physical cues include the geometry of the AFM tips, the indenting force and the operating temperature of the AFM. All of these cues show a significant influence on the cell elasticity assessment. Sharp AFM tips create a two-fold increase in the value of the effective Young’s modulus (Eeff) relative to that of the blunt tips. Higher indenting force at the same loading rate generates higher estimated cell elasticity. Increasing the operation temperature of the AFM leads to decreases in the cell stiffness because the structure of actin filaments becomes disorganized. The physiological cues include the presence of fetal bovine serum or extracellular matrix-coated surfaces, the culture passage number, and the culture density. Both fetal bovine serum and the extracellular matrix are critical for cells to maintain the integrity of actin filaments and consequently exhibit higher elasticity. Unlike primary cells, mouse kidney progenitor cells can be passaged and maintain their morphology and elasticity for a very long period without a senescence phenotype. Finally, cell elasticity increases with increasing culture density only in MDCK epithelial cells. In summary, for researchers who use AFM to assess cell elasticity, our results provide basic and significant information about the suitable selection of physical and physiological cues.
Compressive loading at the end plate directly regulates flow and deformation of the basivertebral vein: an analytical study
Ming-Long Yeh, Michael H Heggeness, Hsiang-Ho Chen, Jennifer Jassawalla, Zong-Ping Luo
Journal of Orthopaedic Surgery and Research , 2006, DOI: 10.1186/1749-799x-1-18
Abstract: A three-dimensional finite element mesh model of the L4 segment with both adjacent discs was modified from a 3-D computed tomography scan image. An octagon representing the basivertebral vein was introduced into the center of the vertebral body in the model. Four compressive orientations (1500 N) were applied on the top disc. The volume change of the vertebral body model and the basivertebral vein were then computed.The volume change of the vertebral body was about 0.1 cm3 (16.3% of the basivertebral vein) for the four loading conditions. The maximum cross-sectional area reductions of the basivertebral vein and volume reduction were 1.54% and 1.02%, for uniform compression.Our study quantified the small but significant volume change of a modeled vertebral body and cross-sectional areas and that of the basivertebral vein, due to the inward bulging of the end plate under compression. This volume change could initiate the reverse flow of blood from the epidural venous system and cause seeding of tumors or bacterial cells.It is well-known that the venous drainage network for the bony vertebral column is unique [1-4]. The caliber of the basivertebral veins of the vertebral body is far out of proportion to similar venous drainage systems for other bones of the body, including large weight-bearing bones [1,5]. Venous blood drains from the basivertebral vein into the epidural system via the central vascular foramen of the vertebral body. This blood then enters the highly compliant epidural system which is continuous with the valveless network of Batson's plexus [1].Metastatic disease and infections frequently involve the spine. This is widely believed to be the result of seeding of the vertebral body by tumor cells or bacteria delivered to the vertebral body by venous blood from Batson's plexus, which is hypothesized to enter the vertebral body via the epidural veins [1-3,6-9]. It has been proposed that during daily activities such as straining, coughing, or lifting with th
Effect of cooling rates on the microstructure of proeutectoid ferrite in Fe-0.76%C alloy under high magnetic field

GONG Ming-Long,

材料研究学报 , 2008,
Low-Power GaAlAs Laser Irradiation Promotes the Proliferation and Osteogenic Differentiation of Stem Cells via IGF1 and BMP2
Jyun-Yi Wu, Yan-Hsiung Wang, Gwo-Jaw Wang, Mei-Ling Ho, Chau-Zen Wang, Ming-Long Yeh, Chia-Hsin Chen
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0044027
Abstract: Low-power laser irradiation (LPLI) has been found to induce various biological effects and cellular processes. Also, LPLI has been shown to promote fracture repair. Until now, it has been unclear how LPLI promotes bone formation and fracture healing. The aim of this study was to investigate the potential mechanism of LPLI-mediated enhancement of bone formation using mouse bone marrow mesenchymal stem cells (D1 cells). D1 cells were irradiated daily with a gallium-aluminum-arsenide (GaAlAs) laser at dose of 0, 1, 2, or 4 J/cm2. The lactate dehydrogenase (LDH) assay showed no cytotoxic effects of LPLI on D1 cells, and instead, LPLI at 4 J/cm2 significantly promoted D1 cell proliferation. LPLI also enhanced osteogenic differentiation in a dose-dependent manner and moderately increased expression of osteogenic markers. The neutralization experiments indicated that LPLI regulated insulin-like growth factor 1 (IGF1) and bone morphogenetic protein 2 (BMP2) signaling to promote cell proliferation and/or osteogenic differentiation. In conclusion, our study suggests that LPLI may induce IGF1 expression to promote both the proliferation and osteogenic differentiation of D1 cells, whereas it may induce BMP2 expression primarily to enhance osteogenic differentiation.
Low-Power Laser Irradiation Suppresses Inflammatory Response of Human Adipose-Derived Stem Cells by Modulating Intracellular Cyclic AMP Level and NF-κB Activity
Jyun-Yi Wu, Chia-Hsin Chen, Chau-Zen Wang, Mei-Ling Ho, Ming-Long Yeh, Yan-Hsiung Wang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0054067
Abstract: Mesenchymal stem cell (MSC)-based tissue regeneration is a promising therapeutic strategy for treating damaged tissues. However, the inflammatory microenvironment that exists at a local injury site might restrict reconstruction. Low-power laser irradiation (LPLI) has been widely applied to retard the inflammatory reaction. The purpose of this study was to investigate the anti-inflammatory effect of LPLI on human adipose-derived stem cells (hADSCs) in an inflammatory environment. We showed that the hADSCs expressed Toll-like Receptors (TLR) 1, TLR2, TLR3, TLR4, and TLR6 and that lipopolysaccharide (LPS) significantly induced the production of pro-inflammatory cytokines (Cyclooxygenase-2 (Cox-2), Interleukin-1β (IL-1β), Interleukin-6 (IL-6), and Interleukin-8 (IL-8)). LPLI markedly inhibited LPS-induced, pro-inflammatory cytokine expression at an optimal dose of 8 J/cm2. The inhibitory effect triggered by LPLI might occur through an increase in the intracellular level of cyclic AMP (cAMP), which acts to down-regulate nuclear factor kappa B (NF-κB) transcriptional activity. These data collectively provide insight for further investigations of the potential application of anti-inflammatory treatment followed by stem cell therapy.
Influence of austenization temperature on the morphology of pearlite in Fe-0.12%C alloy under high magnetic field

Xiang Zhao,Shoujing WANG,GONG Ming-Long,liang zuo,

金属学报 , 2008,
N-acetylcysteine blocked hypoxia-reoxygenation induced apoptosis through ROS-p38 MAPK signaling pathway in neonatal rat cardiomyocytes

Feng-Xiang Zhang,Ming-Long Chen,Bing Yang,Ke-Jiang Cao,

老年心脏病学杂志(英文版) , 2009,
Abstract: Objective Previous investigations have shown that N-aeetylcysteine (NAC) could regulate diverse cell type's apoptosis. The purpose of this study was to evaluate the mechanism of NAC reversed apoptosis ofcardiomyocytes induced by hypoxia-reoxygenation (H/R). Methods Cardiomyocytes were treated with hypoxia 6 h and reoxygenation 72 h in the absence and presence of NAC (100 μmol/ L). The ROS was assayed by using Image-iTTM LIVE green reactive oxygen species detection kit. The viability of cell was assayed with trypan blue. Early stages ofapoptosis were assessed by flow cytometry using Annexin V, and late stages ofapoptosis were assessed using TUNEL system. Bcl2 and bax mRNA levels were determincd by real-time quantitative PCR. Bcl2, bax, p38 and pp38 protein levels were determined by western blot. Results We found that H/R could markedly increase ROS generation and induce the apoptosis of cardiomyocytes (P<0.01). NAC (100 p tool/L) significantly reduced the generation of ROS and apoptosis (P all <0.01). NAC also significantly reduced the protein ratio of pp38 and p38 and increased the RNA and protein ratio of bcl2 and bax (P all <0.01). Conclusion The results showed that NAC significantly reduced apoptosis through inhibiting the phosphorylation of p38 signal pathway, which has potential value for clinical cardiac diseases.
A Cooperative Checkpointing Algorithm with Message Complexity O(n)

WANG Dong-Sheng,SHAO Ming-Long,

软件学报 , 2003,
Abstract: The technology of cooperative checkpointing and rollback recovery as an effective method of fault tolerance, has been widely used on the parallel or distributed computer systems, such as cluster of computers. In order to reduce the overhead of time and space, a cooperative checkpointing algorithm based on message counting is given in this paper. While reducing a message complexity during synchronization from O(n2) to O(n), improving system's efficiency and scalability, this algorithm is also fit for those non-FIFO message passing systems.
Protective effect of guggulsterone against cardiomyocyte injury induced by doxorubicin in vitro
Wang Wen-Ching,Uen Yih-Huei,Chang Ming-Long,Cheah Khoot-Peng
BMC Complementary and Alternative Medicine , 2012, DOI: 10.1186/1472-6882-12-138
Abstract: Background Doxorubicin (DOX) is an effective antineoplastic drug; however, clinical use of DOX is limited by its dose-dependent cardiotoxicity. It is well known that reactive oxygen species (ROS) play a vital role in the pathological process of DOX-induced cardiotoxicity. For this study, we evaluated the protective effects of guggulsterone (GS), a steroid obtained from myrrh, to determine its preliminary mechanisms in defending against DOX-induced cytotoxicity in H9C2 cells. Methods In this study, we used a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) release measurements, and Hoechst 33258 staining to evaluate the protective effect of GS against DOX-induced cytotoxicity in H9C2 cells. In addition, we observed the immunofluorescence of intracellular ROS and measured lipid peroxidation, caspase-3 activity, and apoptosis-related proteins by using Western blotting. Results The MTT assay and LDH release showed that treatment using GS (1–30 μM) did not cause cytotoxicity. Furthermore, GS inhibited DOX (1 μM)-induced cytotoxicity in a concentration-dependent manner. Hoechst 33258 staining showed that GS significantly reduced DOX-induced apoptosis and cell death. Using GS at a dose of 10–30 μM significantly reduced intracellular ROS and the formation of MDA in the supernatant of DOX-treated H9C2 cells and suppressed caspase-3 activity to reference levels. In immunoblot analysis, pretreatment using GS significantly reversed DOX-induced decrease of PARP, caspase-3 and bcl-2, and increase of bax, cytochrome C release, cleaved-PARP and cleaved-caspase-3. In addition, the properties of DOX-induced cancer cell (DLD-1 cells) death did not interfere when combined GS and DOX. Conclusion These data provide considerable evidence that GS could serve as a novel cardioprotective agent against DOX-induced cardiotoxicity.
The Multipartite Mitochondrial Genome of Liposcelis bostrychophila: Insights into the Evolution of Mitochondrial Genomes in Bilateral Animals
Dan-Dan Wei, Renfu Shao, Ming-Long Yuan, Wei Dou, Stephen C. Barker, Jin-Jun Wang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0033973
Abstract: Booklice (order Psocoptera) in the genus Liposcelis are major pests to stored grains worldwide and are closely related to parasitic lice (order Phthiraptera). We sequenced the mitochondrial (mt) genome of Liposcelis bostrychophila and found that the typical single mt chromosome of bilateral animals has fragmented into and been replaced by two medium-sized chromosomes in this booklouse; each of these chromosomes has about half of the genes of the typical mt chromosome of bilateral animals. These mt chromosomes are 8,530 bp (mt chromosome I) and 7,933 bp (mt chromosome II) in size. Intriguingly, mt chromosome I is twice as abundant as chromosome II. It appears that the selection pressure for compact mt genomes in bilateral animals favors small mt chromosomes when small mt chromosomes co-exist with the typical large mt chromosomes. Thus, small mt chromosomes may have selective advantages over large mt chromosomes in bilateral animals. Phylogenetic analyses of mt genome sequences of Psocodea (i.e. Psocoptera plus Phthiraptera) indicate that: 1) the order Psocoptera (booklice and barklice) is paraphyletic; and 2) the order Phthiraptera (the parasitic lice) is monophyletic. Within parasitic lice, however, the suborder Ischnocera is paraphyletic; this differs from the traditional view that each suborder of parasitic lice is monophyletic.
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