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Search Results: 1 - 10 of 1500 matches for " Mika Goto "
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Pitfalls and Remedies in DEA Applications: How to Handle an Occurrence of Zero in Multipliers by Strong Complementary Slackness Conditions  [PDF]
Toshiyuki Sueyoshi, Mika Goto
Engineering (ENG) , 2013, DOI: 10.4236/eng.2013.55A005
Abstract: This study discusses a guideline on a proper use of Data Envelopment Analysis (DEA) that has been widely used for performance analysis in public and private sectors. The use of DEA is equipped with Strong Complementary Slackness Conditions (SCSCs) in this study, but an application of DEA/SCSCs depends upon its careful use, as summarized in the guideline. The guideline consists of the five suggestions. First, a data set used in the DEA applications should not have a ratio variable (e.g., financial ratios) in an input(s) and/or an output(s). Second, radial DEA models under variable and constant Returns to Scale (RTS) need a special treatment on zero in a data set. Third, the DEA evaluation needs to drop an outlier. Fourth, an imprecise number (e.g., 1/3) may suffer from a round-off error because DEA needs to specify it in a precise expression to operate a computer code. Finally, when a large input or output variable may dominate other variables in DEA computation, it is necessary to normalize the data set or simply to divide each observation by its average. Such a simple treatment produces more reliable DEA results than the one without any data adjustment. This study also discusses how to handle an occurrence of zero in DEA multipliers by applying SCSCs. The DEA/SCSCs can serve for a multiplier restriction approach without any prior information. Thus, the propesed DEA/SCSCs can provide more reliable results than a straight use of DEA.
Involvement of miR-214 and miR-375 in Malignant Features of Non-Small-Cell Lung Cancer by Down-Regulating CADM1  [PDF]
Megumi Ishimura, Mika Sakurai-Yageta, Tomoko Maruyama, Tomoko Ando, Masashi Fukayama, Akiteru Goto, Yoshinori Murakami
Journal of Cancer Therapy (JCT) , 2012, DOI: 10.4236/jct.2012.324050
Abstract: A tumor suppressor gene, CADM1, encoding an immunoglobulin superfamily cell adhesion molecule, is inactivated in various cancers, including non-small-cell lung cancer (NSCLC). Although promoter methylation is one of the mechanisms to suppress CADM1 expression, about half of tumors lacking CADM1 expression do not show methylation of the gene promoter. We herein investigated the possible involvement of microRNA (miRNA) in the down-regulation of CADM1. Using computational algorithms, miR-214 and miR-375 were identified as candidate miRNAs targeting CADM1. A luciferase reporter assay demonstrated that miR-214 and miR-375 repressed the promoter activity through 3’-UTR of CADM1. Quantitative RT-PCR analysis demonstrated that miR-214 and miR-375 was highly expressed in 21 (62%) and 17 cases (50%) of 34 primary NSCLCs. Notably, increased expression of miR-214 was preferentially observed in tumors with advanced pathological stages and in those lacking CADM1 expression but were not associated with the promoter methylation, suggesting that miR-214-mediated silencing would be another mechanism to suppress CADM1 expression. On the other hand, introduction of miR-214 or miR-375 into NSCLC cells decreased CADM1 protein expression. Furthermore, overexpression of miR-214 enhanced anchorage-independent growth of NSCLC cells, A549, whereas transfection of miRNA inhibitor, miR-214 or miR-375, significantly suppressed the in vitro wound healing activity of HCC827 cells. These findings suggest that overexpression of miR-214 and miR-375 could participate in the malignant features of NSCLC through down-regulating CADM1 and would provide a potential target for the treatment of a subset of NSCLC.
Resistance coefficient during ice slurry flow through pipe sudden constriction
?. Mika
Archives of Foundry Engineering , 2010,
Abstract: Due to the adverse environmental effects of some commonly-used refrigerants, efforts are still underway to find new cooling mediumsthat would be safer to the ozone layer and would not increase the greenhouse effect. Ice slurry as a new ecological coolant suits theprocesses requiring the preservation of constant and equal temperature in the cooling process of the full section of the cooled solid. Thanks to that, ice slurry can find a wide potential application in such branches of industry, as heat treatment, materials engineering, or foundry. In this paper, flow systems which are commonly used in fittings elements such as diameter’s reductions in ice slurry pipelines, are experimentally investigated. In the study reported in this paper, the consideration was given to the specific features of the slurry flow in which the flow qualities depend mainly on the volume fraction of solid particles. The results of the experimental studies on the flow resistance, presented herein, enabled to determine the resistance coefficient during the ice slurry flow through the pipe sudden constriction. The volume fraction of solid particles in the slurry ranged from 5 to 30%. The recommended and non-recommended range of the Reynolds number for the ice slurry flow through the pipe sudden constriction were presented in this paper. The experimental studies were conducted on a few variants of the most common reductions of copper pipes. Further studies on the determination of the resistance coefficient in the remaining fittings elements of the pipeline were recommended in the paper as well as the further theoretical studies intended to determine the theoretical relations to calculate the resistance coefficient in all the fittings elements in the pipeline (on the basis of the experimental studies) and to elaborate the calculation pattern of the entire ice slurry system.
Cut-Off Points of Head, Chest, and Arm Circumferences to Identify Low Birthweight: Meta-Analysis  [PDF]
Eita Goto
Open Journal of Epidemiology (OJEpi) , 2017, DOI: 10.4236/ojepi.2017.72015
Abstract: Background: The cut-off points of newborn anthropometric variables to identify low birthweight (i.e., birthweight < 2500 g) have varied between studies or even within the same study. Methods: Meta-analysis was performed to summarize cut-off points in studies judged as good quality based on the Quality Assessment of Diagnostic Accuracy Studies tool (QUADAS). PubMed (MEDLINE) and nine other databases were searched (January, 2015). PubMed related-citations and references of potentially eligible articles and related reviews were also investigated. The Egger test was used to assess publication bias. Results: With respect to head, chest, and arm circumferences, the cut-off points that involved no publication bias could be summarized based on the data from large numbers of newborns (=21,793, 8917, and 12,912, respectively) in relatively sufficient numbers of studies (=17, 15, and 19, respectively). The optimal cut-off points to identify low birthweight were 33.0 cm (95% confidence interval [CI], 32.8 - 33.2), 30.4 cm (95% CI, 30.3 - 30.6), and 9.3 cm (95% CI, 9.1 - 9.4) for head circumference, chest circumference, and arm circumference, respectively. The summarized cut-off point of birth height, i.e., 47.2 cm (95% CI, 46.7 - 47.7), used to identify low birthweight involved publication bias (n = 13). Conclusion: The cut-off points were determined to identify low birthweight using head, chest, and arm circumferences.
Uncovering Executive Prioritization: Evaluating Customer Value Propositions with the Pairwise Comparison Method  [PDF]
Mika Yrj?l?
Journal of Service Science and Management (JSSM) , 2015, DOI: 10.4236/jssm.2015.81001
Abstract: Creating customer value is a managerial priority. The role of executives is to choose what type(s) of customer value to propose to customers in the form of a customer value proposition (CVP). The decision is a complex one, because executives have to compare and weigh concrete, measurable elements alongside abstract, subjective ones. The purpose of this paper is to identify themes relating to the use of the pairwise comparison method (PCM) as a tool for prioritizing customer value dimensions from the perspective of retail executives. As a result, this paper first highlights examples of PCM outputs, and second, identifies four themes that capture executive views of the PCM.
Mitochondrial Respiration Is Associated with Alloxan-Induced Mitochondrial Permeability Transition  [PDF]
Koichi Sakurai, Mika Itoh
Journal of Biophysical Chemistry (JBPC) , 2016, DOI: 10.4236/jbpc.2016.74008
Abstract: We previously showed that increased mitochondrial inner membrane permeability which is known as mitochondrial permeability transition (MPT) is triggered by adding succinate in the presence of the diabetogenic agent alloxan. Here, our aim was to investigate whether mitochondrial respiration is associated with alloxan-induced MPT. After mitochondria isolated from rat liver were incubated with alloxan at 37°C for 5 min, the addition of succinate immediately triggered the MPT in the presence of rotenone. However, little or no induction occurred at incubation temperatures below 25°C. Malate/glutamate also triggered MPT by alloxan in the absence of rotenone. In mitochondrial suspensions containing alloxan, succinate accelerated oxygen consumption that was completely inhibited by cyanide. These results suggest that mitochondrial respiration is associated with the alloxan-induced MPT. Alloxan radical production was investigated using ESR spectroscopy. Mitochondria incubated with succinate and alloxan elicited low signal intensity (radical formation) that increased significantly in the presence of cyanide. When the incubation of alloxan with mitochondria after the addition of succinate, a little intensity of the signal was observed, but it was remarkably increased after the addition of cyanide. Ubiquinone analogues inhibited the MPT induction. These results suggest that the initiation of MPT is associated with alloxan redox cycling via an electron transfer process at a quinone-binding site in respiratory mitochondria.
Full-Length Sequence of Mouse Acupuncture-Induced 1-L (Aig1l) Gene Including Its Transcriptional Start Site
Mika Ohta,Aki Sugano,Shuji Goto,Surini Yusoff,Yushi Hirota,Kotaro Funakoshi,Kenji Miura,Eiichi Maeda,Nobuo Takaoka,Nobuko Sato,Hiroshi Ishizuka,Naoki Arizono,Hisahide Nishio,Yutaka Takaoka
Evidence-Based Complementary and Alternative Medicine , 2011, DOI: 10.1093/ecam/nep121
Abstract: We have been investigating the molecular efficacy of electroacupuncture (EA), which is one type of acupuncture therapy. In our previous molecular biological study of acupuncture, we found an EA-induced gene, named acupuncture-induced 1-L (Aig1l), in mouse skeletal muscle. The aims of this study consisted of identification of the full-length cDNA sequence of Aig1l including the transcriptional start site, determination of the tissue distribution of Aig1l and analysis of the effect of EA on Aig1l gene expression. We determined the complete cDNA sequence including the transcriptional start site via cDNA cloning with the cap site hunting method. We then analyzed the tissue distribution of Aig1l by means of northern blot analysis and real-time quantitative polymerase chain reaction. We used the semiquantitative reverse transcriptase-polymerase chain reaction to examine the effect of EA on Aig1l gene expression. Our results showed that the complete cDNA sequence of Aig1l was 6073 bp long, and the putative protein consisted of 962 amino acids. All seven tissues that we analyzed expressed the Aig1l gene. In skeletal muscle, EA induced expression of the Aig1l gene, with high expression observed after 3 hours of EA. Our findings thus suggest that the Aig1l gene may play a key role in the molecular mechanisms of EA efficacy.
An Excellent Monitoring System for Surface Ubiquitination-Induced Internalization in Mammals
Eiji Goto, Mari Mito-Yoshida, Mika Uematsu, Masami Aoki, Yohei Matsuki, Mari Ohmura-Hoshino, Hak Hotta, Makoto Miyagishi, Satoshi Ishido
PLOS ONE , 2008, DOI: 10.1371/journal.pone.0001490
Abstract: Background At present, it is difficult to visualize the internalization of surface receptors induced by ubiquitination that is taken place at the plasma membrane in mammals. This problem makes it difficult to reveal molecular basis for ubiquitination-mediated internalization in mammals. Methodology/Principle Findings In order to overcome it, we have generated T-REx-c-MIR, a novel mammalian Tet-on B cell line using a constitutively active E3 ubiquitin ligase, c-MIR, and its artificial target molecule. By applying the surface biotinylation method to T-REx-c-MIR, we succeeded to monitor the fate of surface target molecules after initiation of ubiquitination process by doxycycline (Dox)-induced c-MIR expression. Target molecules that pre-existed at the plasma membrane before induction of c-MIR expression were oligo-ubiquitinated and degraded by Dox-induced c-MIR expression. Dox-induced c-MIR expression initiated rapid internalization of surface target molecules, and blockage of the internalization induced the accumulation of the surface target molecules that were newly ubiquitinated by c-MIR. Inhibition of the surface ubiquitination by down-regulating ubiquitin conjugating enzyme E2 impaired the internalization of target molecules. Finally, a complex of c-MIR and target molecule was detected at the plasma membrane. Conclusions/Significances These results demonstrate that in T-REx-c-MIR, surface target molecule is ubiquitinated at the plasma membrane and followed by being internalized from the plasma membrane. Thus, T-REx-c-MIR is a useful experimental tool to analyze how surface ubiquitination regulates internalization in mammals.
Coordinated elevation of membrane type 1-matrix metalloproteinase and matrix metalloproteinase-2 expression in rat uterus during postpartum involution
Kengo Manase, Toshiaki Endo, Mitunobu Chida, Kunihiko Nagasawa, Hiroyuki Honnma, Kiyohiro Yamazaki, Yoshimitu Kitajima, Taeko Goto, Mika Kanaya, Takuhiro Hayashi, Toshihiro Mitaka, Tsuyoshi Saito
Reproductive Biology and Endocrinology , 2006, DOI: 10.1186/1477-7827-4-32
Abstract: We performed gelatin zymography, northern blot analysis and immunohistochemistry to compare the expression levels of MT1-MMP, MMP-2, matrix metalloproteinase-9 (MMP-9) and the tissue inhibitors of MMPs-1 and 2 (TIMP-1 and TIMP-2) in the rat uterus 18 h, 36 h and 5 days after parturition with their expression levels during pregnancy (day 20).We found that both MT1-MMP and MMP-2 localized mainly in the cytoplasm of uterine interstitial cells. The expression levels of MT1-MMP and MMP-2 mRNAs and the catalytic activities of the expressed proteins significantly increased 18 h and 36 h after parturition, but at postpartum day 5, their mRNA expression levels and catalytic activities decreased markedly. The expression levels of MMP-9 increased 18 h and 36 h after parturition as determined by gelatin zymography including the expression levels of TIMP-1 and TIMP-2.These expression patterns indicate that MT1-MMP, MMP-2, MMP-9, TIMP-1 and TIMP-2 may play key roles in uterine postpartum involution and subsequent functional regenerative processes.During pregnancy, the uterus enlarges, which in rats is mainly caused by an increase in the amount of collagen and hypertrophy of the uterine smooth muscle cells. After parturition, the uterus undergoes involution during which it returns to its prepregnancy state. Matrix metalloproteinases (MMPs) are a group of structurally related endopeptidases that catalyze the degradation of various macromolecular components of the extracellular matrix and basement membrane [1,2], and induce various forms of tissue remodeling, including wound healing [3,4], trophoblast invasion [5,6], organ morphogenesis [7,8], and uterine [9-11], mammary gland [12,13], and prostate gland [14,15] involution. We previously reported that an increase in the expression levels of both membrane type 1-MMP (MT1-MMP) and MMP-2 plays a key role in tissue remodeling during corpus luteum structural involution both in rats and humans [16-18].To obtain additional information on t
Preparation and Properties of Polyaniline in the Presence of Trehalose  [PDF]
Hirotsugu Kawashima, Hiromasa Goto
Soft Nanoscience Letters (SNL) , 2011, DOI: 10.4236/snl.2011.13013
Abstract: Oxidative polymerization of aniline in aqueous solution in the presence of trehalose was conducted. Fourier transform infrared spectroscopy confirmed successful preparation of polyaniline containing a trace amount of trehalose. Electron spin resonance spectroscopy revealed that electron spin concentration of the polyaniline increases with aniline/trehalose ratio in the polymerization. Scanning electron microscopy revealed that the polyaniline shows granular and porous morphology. Electrical conductivity of these polyanilines was in the order of 10-4 S/cm.
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