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Search Results: 1 - 10 of 219633 matches for " Meagan C Burnet "
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Experimental annotation of post-translational features and translated coding regions in the pathogen Salmonella Typhimurium
Charles Ansong, Nikola Toli?, Samuel O Purvine, Steffen Porwollik, Marcus Jones, Hyunjin Yoon, Samuel H Payne, Jessica L Martin, Meagan C Burnet, Matthew E Monroe, Pratap Venepally, Richard D Smith, Scott N Peterson, Fred Heffron, Michael McClelland, Joshua N Adkins
BMC Genomics , 2011, DOI: 10.1186/1471-2164-12-433
Abstract: We experimentally annotated the bacterial pathogen Salmonella Typhimurium 14028, using "shotgun" proteomics to accurately uncover the translational landscape and post-translational features. The data provide protein-level experimental validation for approximately half of the predicted protein-coding genes in Salmonella and suggest revisions to several genes that appear to have incorrectly assigned translational start sites, including a potential novel alternate start codon. Additionally, we uncovered 12 non-annotated genes missed by gene prediction programs, as well as evidence suggesting a role for one of these novel ORFs in Salmonella pathogenesis. We also characterized post-translational features in the Salmonella genome, including chemical modifications and proteolytic cleavages. We find that bacteria have a much larger and more complex repertoire of chemical modifications than previously thought including several novel modifications. Our in vivo proteolysis data identified more than 130 signal peptide and N-terminal methionine cleavage events critical for protein function.This work highlights several ways in which application of proteomics data can improve the quality of genome annotations to facilitate novel biological insights and provides a comprehensive proteome map of Salmonella as a resource for systems analysis.Many aspects of modern biological research are dependent on accurate identification of the protein-coding genes in each genome, as well as the nature of the mature functional protein products, a process commonly referred to as genome annotation. With the exponential increase in the number of sequenced prokaryotic genomes afforded by advances in genome sequencing technologies over the last decade, present day prokaryotic genome annotation is essentially an automated high-throughput process that relies heavily on de novo gene prediction programs [1-3].While de novo gene prediction programs have significantly improved for prokaryotic genomes consider
Structural and Functional Characterization of DUF1471 Domains of Salmonella Proteins SrfN, YdgH/SssB, and YahO
Alexander Eletsky, Karolina Michalska, Scott Houliston, Qi Zhang, Michael D. Daily, Xiaohui Xu, Hong Cui, Adelinda Yee, Alexander Lemak, Bin Wu, Maite Garcia, Meagan C. Burnet, Kristen M. Meyer, Uma K. Aryal, Octavio Sanchez, Charles Ansong, Rong Xiao, Thomas B. Acton, Joshua N. Adkins, Gaetano T. Montelione, Andrzej Joachimiak, Cheryl H. Arrowsmith, Alexei Savchenko, Thomas Szyperski, John R. Cort
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0101787
Abstract: Bacterial species in the Enterobacteriaceae typically contain multiple paralogues of a small domain of unknown function (DUF1471) from a family of conserved proteins also known as YhcN or BhsA/McbA. Proteins containing DUF1471 may have a single or three copies of this domain. Representatives of this family have been demonstrated to play roles in several cellular processes including stress response, biofilm formation, and pathogenesis. We have conducted NMR and X-ray crystallographic studies of four DUF1471 domains from Salmonella representing three different paralogous DUF1471 subfamilies: SrfN, YahO, and SssB/YdgH (two of its three DUF1471 domains: the N-terminal domain I (residues 21–91), and the C-terminal domain III (residues 244–314)). Notably, SrfN has been shown to have a role in intracellular infection by Salmonella Typhimurium. These domains share less than 35% pairwise sequence identity. Structures of all four domains show a mixed α+β fold that is most similar to that of bacterial lipoprotein RcsF. However, all four DUF1471 sequences lack the redox sensitive cysteine residues essential for RcsF activity in a phospho-relay pathway, suggesting that DUF1471 domains perform a different function(s). SrfN forms a dimer in contrast to YahO and SssB domains I and III, which are monomers in solution. A putative binding site for oxyanions such as phosphate and sulfate was identified in SrfN, and an interaction between the SrfN dimer and sulfated polysaccharides was demonstrated, suggesting a direct role for this DUF1471 domain at the host-pathogen interface.
The Value of Color Research in Brand Strategy  [PDF]
Meagan K. Cunningham
Open Journal of Social Sciences (JSS) , 2017, DOI: 10.4236/jss.2017.512014
Abstract:
Is color more than a design? The following research discusses the science of color to consumer perception and the value of that color research to consumer-brand relationships. Specifically, it examines how color influences consumers’ perception and how brands strategically utilize color to distinguish themselves amongst competitors, establish an identity, promote an image, and foster relationships with its consumers. To examine the significance of color to consumer perception and brand imagery, a nonrandom sample of men and women between the ages of 18 and 37 years old participated in a focus group that included color-centric photos and a survey. The results of the nonrandom sample also show that colors used in branding influence consumers’ perception, are used to identify products, and carry meaning that’s evolved into a relationship between the brand and the consumer. These findings were consistent with previous research that found through color, brands effectively establish an identity, communicate a mood, and form a relationship with consumers (Labrecque and Milne, 2011) [1]. The results of the survey also reveal that consumers don’t rely solely on color when recognizing a branding and more can be done to examine how integral color is to manufacturing brand-consumer relationships.
Action Research and Music Therapy: Group Music Therapy with Young Refugees in a School Community
Meagan Hunt
Voices: A World Forum for Music Therapy , 2005,
Abstract:
Post-Operative Radiotherapy for Soft Tissue Sarcoma of the Anterior Compartment of the Thigh: Should the Sartorius Muscle be Included?
Gillian C. Barnett,Andrew C. F. Hoole,Nicola Twyman,Sarah J. Jefferies,Neil G. Burnet
Sarcoma , 2005, DOI: 10.1080/13577140400025961
Abstract: Purpose: The clinical target volume (CTV) of post-operative radiotherapy for soft tissue sarcoma of the limbs conventionally includes the whole of the transverse cross-section of the affected anatomical compartment. In the anterior thigh sartorius appears to lie within its own fascial compartment and can be safely excluded. We investigated the potential impact of omitting sartorius from the anterior muscle compartment on patients with soft tissue sarcoma of the thigh.
Conformal Radiotherapy Facilitates the Delivery of Concurrent Chemotherapy and Radiotherapy: A Case of Primitive Neuroectodermal Tumour of the Chest Wall
C. E. Coles,N. Twyman,H. M. Earl,N. G. Burnet
Sarcoma , 2000, DOI: 10.1080/13577140020008101
Abstract: We illustrate the principle of conformal radiotherapy by discussing the case of a patient with a primitive neuroectodermal tumour of the chest wall. Recent advances in radiotherapy planning enable precise localization of the planning target volume (PTV) and normal organs at risk of irradiation. Customized blocks are subsequently designed to produce a treatment field that ‘conforms’ to the PTV. The use of conformal radiotherapy (CRT) in this case facilitated the delivery of concurrent chemotherapy and radiotherapy by significantly reducing the volume of red marrow irradiated.The lack of acute and late toxicities was attributed to optimal exclusion of normal tissues from the treatment field, made possible by CRT.
The CORALIE survey for southern extrasolar planets V: 3 new extrasolar planets
D. Naef,M. Mayor,F. Pepe,D. Queloz,N. C. Santos,S. Udry,M. Burnet
Physics , 2001, DOI: 10.1051/0004-6361:20010841
Abstract: We report the detection of 3 new planetary companions orbiting the solar-type stars GJ 3021, HD 52265 and HD 169830 using radial-velocity measurements taken with the CORALIE echelle spectrograph. All these planetary companions have longer orbital periods than the 51 Peg-like objects. The orbits are fairly eccentric. The minimum masses of these planets range from 1 to 3.3 M_Jup. The stars have spectral types from F8V to G6V. They are metal-rich. We also present our radial-velocity measurements for three solar-type stars known to host planetary companions iota Hor (HD 17051), HD 210277 and HD 217107.
The CORALIE survey for southern extra-solar planets VIII. The very low-mass companions of HD141937, HD162020, HD168443, HD202206: brown dwarfs or superplanets?
S. Udry,M. Mayor,D. Naef,F. Pepe,D. Queloz,N. C. Santos,M. Burnet
Physics , 2002, DOI: 10.1051/0004-6361:20020685
Abstract: Doppler CORALIE measurements of the solar-type stars HD141937, HD162020, HD168443 and HD202206 show Keplerian radial-velocity variations revealing the presence of 4 new companions with minimum masses close to the planet/brown-dwarf transition, namely with m_2sin(i) = 9.7, 14.4, 16.9, and 17.5 M_Jup, respectively. The orbits present fairly large eccentricities (0.22
Potential for Rabies Control through Dog Vaccination in Wildlife-Abundant Communities of Tanzania
Meagan C. Fitzpatrick ,Katie Hampson,Sarah Cleaveland,Lauren Ancel Meyers,Jeffrey P. Townsend,Alison P. Galvani
PLOS Neglected Tropical Diseases , 2012, DOI: 10.1371/journal.pntd.0001796
Abstract: Canine vaccination has been successful in controlling rabies in diverse settings worldwide. However, concerns remain that coverage levels which have previously been sufficient might be insufficient in systems where transmission occurs both between and within populations of domestic dogs and other carnivores. To evaluate the effectiveness of vaccination targeted at domestic dogs when wildlife also contributes to transmission, we applied a next-generation matrix model based on contract tracing data from the Ngorongoro and Serengeti Districts in northwest Tanzania. We calculated corresponding values of R0, and determined, for policy purposes, the probabilities that various annual vaccination targets would control the disease, taking into account the empirical uncertainty in our field data. We found that transition rate estimates and corresponding probabilities of vaccination-based control indicate that rabies transmission in this region is driven by transmission within domestic dogs. Different patterns of rabies transmission between the two districts exist, with wildlife playing a more important part in Ngorongoro and leading to higher recommended coverage levels in that district. Nonetheless, our findings indicate that an annual dog vaccination campaign achieving the WHO-recommended target of 70% will control rabies in both districts with a high level of certainty. Our results support the feasibility of controlling rabies in Tanzania through dog vaccination.
Impact of Ribosomal Modification on the Binding of the Antibiotic Telithromycin Using a Combined Grand Canonical Monte Carlo/Molecular Dynamics Simulation Approach
Meagan C. Small,Pedro Lopes,Rodrigo B. Andrade,Alexander D. MacKerell Jr
PLOS Computational Biology , 2013, DOI: 10.1371/journal.pcbi.1003113
Abstract: Resistance to macrolide antibiotics is conferred by mutation of A2058 to G or methylation by Erm methyltransferases of the exocyclic N6 of A2058 (E. coli numbering) that forms the macrolide binding site in the 50S subunit of the ribosome. Ketolides such as telithromycin mitigate A2058G resistance yet remain susceptible to Erm-based resistance. Molecular details associated with macrolide resistance due to the A2058G mutation and methylation at N6 of A2058 by Erm methyltransferases were investigated using empirical force field-based simulations. To address the buried nature of the macrolide binding site, the number of waters within the pocket was allowed to fluctuate via the use of a Grand Canonical Monte Carlo (GCMC) methodology. The GCMC water insertion/deletion steps were alternated with Molecular Dynamics (MD) simulations to allow for relaxation of the entire system. From this GCMC/MD approach information on the interactions between telithromycin and the 50S ribosome was obtained. In the wild-type (WT) ribosome, the 2′-OH to A2058 N1 hydrogen bond samples short distances with a higher probability, while the effectiveness of telithromycin against the A2058G mutation is explained by a rearrangement of the hydrogen bonding pattern of the 2′-OH to 2058 that maintains the overall antibiotic-ribosome interactions. In both the WT and A2058G mutation there is significant flexibility in telithromycin's imidazole-pyridine side chain (ARM), indicating that entropic effects contribute to the binding affinity. Methylated ribosomes show lower sampling of short 2′-OH to 2058 distances and also demonstrate enhanced G2057-A2058 stacking leading to disrupted A752-U2609 Watson-Crick (WC) interactions as well as hydrogen bonding between telithromycin's ARM and U2609. This information will be of utility in the rational design of novel macrolide analogs with improved activity against methylated A2058 ribosomes.
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