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Search Results: 1 - 10 of 337 matches for " Masakazu Toi "
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Tumor Angiogenesis: Pericytes and Maturation Are Not to Be Ignored
Elham Fakhrejahani,Masakazu Toi
Journal of Oncology , 2012, DOI: 10.1155/2012/261750
Abstract: Angiogenesis, an essential component of tumor growth and survival, is regulated by complex interactions between several cell types and soluble mediators. Heterogeneous tumor vasculature originates from the collective effect of the nature of carcinoma and the complexity of the angiogenic network. Although the application of angiogenesis inhibitors in some types of cancers has shown clinical benefits, predictive markers to assess treatment effects have yet to be established. In this review, we focus on tumor vessel maturity as a potential marker for evaluating treatment response.
Computational Modeling of Induction Hardening Process of Machine Parts
Yutaka Toi,Masakazu Takagaki
Lecture Notes in Engineering and Computer Science , 2008,
Trastuzumab Produces Therapeutic Actions by Upregulating miR-26a and miR-30b in Breast Cancer Cells
Takehiro Ichikawa, Fumiaki Sato, Kazuya Terasawa, Soken Tsuchiya, Masakazu Toi, Gozoh Tsujimoto, Kazuharu Shimizu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0031422
Abstract: Objective Trastuzumab has been used for the treatment of HER2-positive breast cancer (BC). However, a subset of BC patients exhibited resistance to trastuzumab therapy. Thus, clarifying the molecular mechanism of trastuzumab treatment will be beneficial to improve the treatment of HER2-positive BC patients. In this study, we identified trastuzumab-responsive microRNAs that are involved in the therapeutic effects of trastuzumab. Methods and Results RNA samples were obtained from HER2-positive (SKBR3 and BT474) and HER2-negetive (MCF7 and MDA-MB-231) cells with and without trastuzumab treatment for 6 days. Next, we conducted a microRNA profiling analysis using these samples to screen those microRNAs that were up- or down-regulated only in HER2-positive cells. This analysis identified miR-26a and miR-30b as trastuzumab-inducible microRNAs. Transfecting miR-26a and miR-30b induced cell growth suppression in the BC cells by 40% and 32%, respectively. A cell cycle analysis showed that these microRNAs induced G1 arrest in HER2-positive BC cells as trastuzumab did. An Annexin-V assay revealed that miR-26a but not miR-30b induced apoptosis in HER2-positive BC cells. Using the prediction algorithms for microRNA targets, we identified cyclin E2 (CCNE2) as a target gene of miR-30b. A luciferase-based reporter assay demonstrated that miR-30b post-transcriptionally reduced 27% (p = 0.005) of the gene expression by interacting with two binding sites in the 3′-UTR of CCNE2. Conclusion In BC cells, trastuzumab modulated the expression of a subset of microRNAs, including miR-26a and miR-30b. The upregulation of miR-30b by trastuzumab may play a biological role in trastuzumab-induced cell growth inhibition by targeting CCNE2.
A Novel Method for Sentinel Lymph Node Biopsy by Indocyanine Green Fluorescence Technique in Breast Cancer
Tomoharu Sugie,Kassim Abdelazeem Kassim,Megumi Takeuchi,Takashi Hashimoto,Kazuhiko Yamagami,Yoshikazu Masai,Masakazu Toi
Cancers , 2010, DOI: 10.3390/cancers2020713
Abstract: We investigated the feasibility of sentinel lymph node (SLN) biopsy using indocyanine green (ICG) technique in 411 patients with early breast cancer at three institutes. ICG, a fluorescence source, and blue dye were injected into the subareolar area to enable real-time image-guided surgery and identification of SLN fluorescence after meticulous dissection. The subcutaneous lymphatic channels were precisely detected in all cases. SLN identification rate was 99% (408/411) with a mean of 2.3 nodes identified per patient. Thirty-nine cases (9.5%) had SLNs involved and all of them were ICG positive. Thus, the ICG technique has a high SLN identification rate comparable with that of the radioisotope method.
The reversal of recurrence hazard rate between ER positive and negative breast cancer patients with axillary lymph node dissection (pathological stage I-III) 3 years after surgery
Takayoshi Kiba, Takashi Inamoto, Tsutomu Nishimura, Masaya Ueno, Kazuhiro Yanagihara, Satoshi Teramukai, Hironori Kato, Masakazu Toi, Masanori Fukushima
BMC Cancer , 2008, DOI: 10.1186/1471-2407-8-323
Abstract: This study investigated the assessment of variables in defining prognosis of 742 breast cancer women with pathological stage (pTNM) I-III diagnosed between 1980 and 2005 at the Kyoto University Hospital in Japan, by age, clinical stage (cTNM), pTNM, the numbers of positive lymph nodes (pN), and ER status.Multivariate analysis demonstrated that pTNM and ER status were the independent prognostic factors for overall survival, and that pTNM and pN were the independent prognostic factors for disease-free survival. For the 0- to 2-year interval, the hazard of recurrence was higher for the ER-negative patients than the ER-positive patients, and beyond 3 years the hazard was higher for ER-positive patients.The present study confirmed the previous reports which showed favorable prognosis of the patients with lesser pTNM or positive ER status. A reversal of recurrence hazard rate between ER positive and negative breast cancer patients beyond 3 years after operation was detected. The fact may indicate the importance of long term adjuvant hormone therapy for ER positive cancer patients.A prognostic factor is defined as a biological or clinical measurement that is associated with overall survival and/or disease-free survival [1]. The knowledge of prognosis forms an integral part of the decision-making process in medicine [2]. Moreover, prognostic factors are important in the treatment of cancer to help identify subgroups of patients who may need more aggressive approach to therapy [3]. Further, prognostic factors also play a critical role in designing clinical trial as stratification and allocation factors [4]. Prognostic factors, i.e., those that predict the risk of recurrence or death from breast cancer, include stage, number of positive axillary nodes, tumor size, lymphatic and vascular invasion, the estrogen-receptor (ER) and progesterone-receptor (PR) positivity, and HER2/neu gene amplification [3,5]. We previously reported that the recent advance of the survival rates in b
Prediction of axillary lymph node metastasis in primary breast cancer patients using a decision tree-based model
Masahiro Takada, Masahiro Sugimoto, Yasuhiro Naito, Hyeong-Gon Moon, Wonshik Han, Dong-Young Noh, Masahide Kondo, Katsumasa Kuroi, Hironobu Sasano, Takashi Inamoto, Masaru Tomita, Masakazu Toi
BMC Medical Informatics and Decision Making , 2012, DOI: 10.1186/1472-6947-12-54
Abstract: Clinical datasets for primary breast cancer patients who underwent sentinel lymph node biopsy or AxLN dissection without prior treatment were collected from three institutes (institute A, n?=?148; institute B, n?=?143; institute C, n?=?174) and were used for variable selection, model training and external validation, respectively. The models were evaluated using area under the receiver operating characteristics (ROC) curve analysis to discriminate node-positive patients from node-negative patients.The ADTree model selected 15 of 24 clinicopathological variables in the variable selection dataset. The resulting area under the ROC curve values were 0.770 [95% confidence interval (CI), 0.689–0.850] for the model training dataset and 0.772 (95% CI: 0.689–0.856) for the validation dataset, demonstrating high accuracy and generalization ability of the model. The bootstrap value of the validation dataset was 0.768 (95% CI: 0.763–0.774).Our prediction model showed high accuracy for predicting nodal metastasis in patients with breast cancer using commonly recorded clinical variables. Therefore, our model might help oncologists in the decision-making process for primary breast cancer patients before starting treatment.
The Validity of the Reduction of Disposable Plastic Checkout Bags in Japan  [PDF]
Masakazu Yamashita, Daichi Toyofuku
Journal of Environmental Protection (JEP) , 2012, DOI: 10.4236/jep.2012.39118
Abstract: This study examined the effect of reducing disposable plastic checkout bags used in supermarkets, convenience stores, and so on in Japan. Considering that even when these checkout bags are abolished, alternative waste bags should be newly produced, because these checkout bags have been reused as household waste bags so far, and the corresponding amount of oil is still necessary to produce them, the amount of oil saved by this bag reduction was found to be 0.2 L/person/year at most. Further, it was demonstrated that the necessity to purchase substitute bags may increase the household and financial burden on consumers.
Possibility of the Benzoic Acids Formation by Oxidation of Benzyl Chlorides in the Sand  [PDF]
Masakazu Yamashita, Masato Hata
Journal of Environmental Protection (JEP) , 2013, DOI: 10.4236/jep.2013.41014

Seeking the possibility of the formation of benzoic acids from benzyl halides in natural soil, a model oxidation reaction of the halides was carried out in the presence of water and soil in an open air under UV light irradiation. Addition of TiO2 to soil raised the yield of the acid, implying such a metal oxide plays a real catalytic role in this oxidation. By these supplementary experiments, one of the possibilities for the formation of benzoic acids in natural soil from benzyl chlorides was supported.

Dust Resulting from Tire Wear and the Risk of Health Hazards  [PDF]
Masakazu Yamashita, Shohei Yamanaka
Journal of Environmental Protection (JEP) , 2013, DOI: 10.4236/jep.2013.46059

The present study examined the effects of air pollutants on people’s health, focusing on dust produced from automobile tires while cars drive on roads. The annual volume of dust resulting from tire wear, calculated based on the number of automobiles registered in Japan, was 1747245.4 m3. To put it simply, this translates to approximately 1.4 times the volume of the Tokyo Dome, a famous Japanese baseball stadium. Particulate substances are categorized into three groups depending on their size, and dust resulting from tire wear is classified into the coarse particle mode along with mold spores, pollen, and dust produced from brake pads. This study examined whether or not tire dust causes health damage similarly to pollen, a particulate substance in the same group. There were 38/cm2 dust particles resulting from tire wear on a busy road in Osaka Prefecture, and this number was larger than that of cedar pollen/cm2 (35), a cause of hay fever, identified in Hokkaido. The results suggest that tire dust may also adversely affect the health of people if any of its constituents has a toxicity or causes allergies.

Effects of Heavy Metal Pollution of Apple Orchard Surface Soils Associated with Past Use of Metal-Based Pesticides on Soil Microbial Biomass and Microbial Communities  [PDF]
Masakazu Aoyama, Ryo Tanaka
Journal of Environmental Protection (JEP) , 2013, DOI: 10.4236/jep.2013.44A005

Apple orchard surface soils in Japan are polluted with copper (Cu), lead (Pb), and arsenic (As) due to long-term use of metal-based pesticides. We investigated the effects of heavy metals accumulated in the surface soils in apple orchards on the microbial biomass and the microbial communities. Soil samples were taken from a chestnut orchard (unpolluted control) and five apple orchards with different degrees of heavy metal pollution. Total concentrations of Cu, Pb, and As in soil ranged from 29 to 931 mg/kg, 35 to 771 mg/kg, and 11 to 198 mg/kg, respectively. The amount of microbial biomass carbon expressed on a soil organic carbon basis decreased with increasing concentrations of heavy metals. Thus, the heavy metals that accumulated in apple orchard surface soils had adverse effects on the soil microbial biomass. The analysis of phospholipid fatty acid (PLFA) composition indicated that the microbial community structure had changed because of the pesticide-derived heavy metals in soil. The relative abundance of gram-positive bacterial marker PLFAs increased and that of fungal marker PLFA decreased with increasing concentrations of heavy metals in soil. Denaturing gradient gel electrophoreses targeting the 16S ribosomal RNA gene of bacteria and the 18S ribosomal RNA gene of fungi also showed shifts in the composition of bacterial and fungal communities induced by soil pollution with heavy metals. However, the diversity of microbial communities was not significantly affected by the heavy metal pollution. This was attributable to the adaptation of the microbial communities in apple orchard surface soils to heavy metals derived from previously used pesticides.

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