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Search Results: 1 - 10 of 5503 matches for " Mary Haverbusch "
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Quality assessment of buccal versus blood genomic DNA using the Affymetrix 500 K GeneChip
Jessica G Woo, Guangyun Sun, Mary Haverbusch, Subbarao Indugula, Lisa J Martin, Joseph P Broderick, Ranjan Deka, Daniel Woo
BMC Genetics , 2007, DOI: 10.1186/1471-2156-8-79
Abstract: Buccal cytobrushes stored for ~7 years at -80°C prior to extraction yielded sufficient double stranded DNA (dsDNA) to be successfully genotyped on the Affymetrix ~262 K NspI chip, with yields between 536 and 1047 ng dsDNA. Using the BRLMM algorithm, genotyping call rates for blood samples averaged 98.4%, and for buccal samples averaged 97.8%. Matched blood samples exhibited 99.2% concordance, while matched blood and buccal samples exhibited 98.8% concordance.Buccal cytobrushes stored long-term result in sufficient dsDNA concentrations to achieve high genotyping call rates and concordance with stored blood samples in the context of Affymetrix 500 K SNP genotyping. Thus, given high-quality collection and storage protocols, it is possible to use stored buccal cytobrush samples for genome-wide association studies.While blood is considered the optimal source for DNA, inclusion of a blood draw may deter study participation [1]. Buccal cytobrush collection is a simple, painless procedure that allows for effective DNA sampling from a large population, and has been used in several large epidemiologic studies [2,3]. However, concerns regarding the use of buccal brushes have included the lower quantity of genomic DNA isolated [4], lower quality of DNA [4,5], and the fidelity of results from buccal brushes compared with blood samples [5-7]. In addition, there is a concern that older buccal brush samples may not yield as high-quality results as fresh samples [8].The advent of large scale genotyping platforms has also resulted in a reduction in the amount of DNA required. The Affymetrix 500 K GeneChip requires only 250 ng of total genomic DNA per chip, 500 ng total, and this DNA quantity has not changed with the recent release of the Affymetrix 5.0 and 6.0 chips, which enable genotyping up to 1.8 million genetic markers [9-11]. Thus, the DNA requirements of the Affymetrix chips are well below the expected yield of total DNA for buccal samples. As the Affymetrix system uses restri
Subarachnoid hemorrhage: tests of association with apolipoprotein E and elastin genes
Ritesh Kaushal, Daniel Woo, Prodipto Pal, Mary Haverbusch, Huifeng Xi, Charles Moomaw, Padmini Sekar, Brett Kissela, Dawn Kleindorfer, Matthew Flaherty, Laura Sauerbeck, Ranajit Chakraborty, Joseph Broderick, Ranjan Deka
BMC Medical Genetics , 2007, DOI: 10.1186/1471-2350-8-49
Abstract: At the APOE locus, no individual SNP was associated with SAH after correction for multiple comparisons. Haplotype analysis revealed significant association of the major haplotype (Hap1) in APOE with SAH (p = 0.001). The association stemmed from both the 5' promoter and the 3' region of the APOE gene. APOE ε2 and ε 4 were not significantly associated with SAH. No association was observed for ELN at genotype, allele, or haplotype level and our study failed to confirm previous reports of ELN association with aneurysmal SAH.This study suggests a role of the APOE gene in the etiology of aneurysmal SAH.Non-traumatic, spontaneous subarachnoid hemorrhage (SAH) affects 16,000 to 17,000 individuals each year in the United States [1-3]. SAH has a 30-day mortality rate exceeding 40%, and surviving patients often demonstrate significant morbidity [2,4]. Over 80% of SAH can be attributed to intracranial aneurysm (IA) rupture. Familial aggregation studies of SAH have consistently identified an increased risk of a first-degree relative with SAH or family history of SAH independent of smoking and hypertension [5].Variants of the apolipoprotein E (APOE) gene have been associated with Alzheimer's disease, lipid disorders and cardiovascular disease [6-8]. Previous studies have demonstrated that APOE ε4 and/or APOE ε2 are associated with lobar intracerebral hemorrhage (ICH) [9,10]. We recently reported that haplotypes which include polymorphisms in the 5' untranslated region of the APOE gene are risk factors for lobar ICH [11]. Specific to SAH, Kokubo et al. [12] found significant association of APOE ε4 with SAH in a Japanese population. Niskakangas et al. [13] reported association of APOE ε4 with adverse outcome after aneurysmal SAH. No study on other polymorphisms of APOE with regard to risk of SAH has yet been reported.In addition to APOE, the elastin (ELN) gene emerged as a putative gene for IA after linkage was found on 7q11, where ELN is located [14]. However, prior association st
Gender Differences in Attitudes to Ageing among Norwegian Older Adults  [PDF]
Mary Kalfoss
Open Journal of Nursing (OJN) , 2016, DOI: 10.4236/ojn.2016.63026
Abstract: Increased life expectancy has led to policy interest in adding quality to years of life and in related concepts such as attitudes to ageing. Improving attitudes to ageing is regarded as one means of improving the participation and contribution of older people within society. In considering age- related attitudes in the dynamic nature of social identity, age is not just one social category that may or may not constitute a part of one’s identity. Other identities such as gender may be more salient in attitudes to ageing. The purpose of this study was to explore Norwegian gender differences in attitudes to ageing among 282 females and 200 males living in the community. Attitudes to ageing were measured using the Attitudes to Ageing Questionnaire (AAQ; Laidlaw, Power, Schmidt, & the WHOQOL-OLD Group, 2007). Both genders in this study had positive attitudes toward their ageing, in spite of acknowledging loss with problems of exclusion, making friends and talking about difficult feelings. Compared to men, women perceived ageing as a time of greater loss, however, they felt more strongly that ageing brought wisdom and that their life had made a difference. On the other hand, men felt more strongly that physical problems did not hold them back from doing what they wanted to do and believed more strongly that they passed on their experiences to others. Both genders felt that their identity was not defined by age, they didn’t feel old, aging was a privilege, and appraised the importance of exercising. Attitudes to ageing are becoming increasingly important in ageing societies. Such positive attitudes should be regarded with importance to health professionals, leaders and policy makers in planning interventions to buffer the detrimental aspects of ageing.
Student’s Perception of Missed Care: Focus Group Results  [PDF]
Mary Kalfoss
Open Journal of Nursing (OJN) , 2017, DOI: 10.4236/ojn.2017.77064
Abstract: Background: With the inflation of economic constraints on health care and demand to increase care quality, there is an increasing need to develop a clear understanding of what actions by health professionals are perceived as threatening quality care. Objective: To explore graduate nursing and pastoral care student’s perceptions of missed care in Norway. Research design: A qualitative study was employed with the formation of six focus groups. Data was analyzed via a thematic content of the discussions. Participants and research context: Thirty-one students attending a University College in Oslo participated. Findings: Five major themes and thirty subthemes were identified. Major themes included labor constraints, organizational contraints, professional constraints, communication constaints and emotional strain. Discussion: Findings of this study resonate with other research as well as with studies on missed nursing care. Findings also lend support to the definition of missed nursing care actions as required care that is omitted, either in part or whole, or delayed. Conclusion: The findings from this study extend understanding of what barriers health professionals perceive as inhibiting them from offering quality care. The focus groups provided a valuable flora for discussion regarding what participants perceived as missed.
Prayer for Good Governance: A Study of Psalm 72 in the Nigeria Context  [PDF]
Mary Jerome Obiorah
Open Journal of Philosophy (OJPP) , 2013, DOI: 10.4236/ojpp.2013.31A032

Contextualizationof Biblical texts is a priority of every exegete, who endeavors to bring the ancient scripts to dialogue with contemporary issues. This paper, which studies Psalm 72 and a prayer composed for good governance in Anambra State Nigeria, focuses on this hermeneutical interpretation. The writer adopts a simplified literary method in Biblical research that takes cognizance of the varied poetic techniques in Psalm 72 and engages in a detailed comparative study of a Psalm composed more than two millennia ago and a prayer of our time. Such comparative study reveals a striking similar preoccupation of the composers of both texts. Both prayed for good governance of which all developing countries today are in dire need.

Factors associated with relapse and remission of alcohol dependent persons after community based treatment  [PDF]
Mary Wangari Kuria
Open Journal of Psychiatry (OJPsych) , 2013, DOI: 10.4236/ojpsych.2013.32025

Knowledge of factors associated with relapse and remission after treatment for alcohol dependence enables the clinician to offer better individualized treatment. It also enables the clinician to predict which patients are likely to relapse and therefore offer appropriate and effective treatment to prevent relapse. Objective: This study sought to determine the factors associated with remission and relapse in a group of alcohol dependent persons undergoing Community Based Detoxification and Rehabilitation of alcohol dependent persons. Method: One hundred and eighty eight (188) persons with Alcohol Use Disorder Identification Test (AUDIT) positive were subjected to outpatient detoxification for 10 days using a pair of ampoules of high potency Vitamin B and C intravenously daily for 3 consecutive days, diazepam 5 mg and carbamazepine 200 mg for 5 and 10 consecutive nights respectively on an outpatient basis. The participants were visited twice a week (at home) by the community based health workers and reviewed once a week by the principal investigator and attended a bimonthly group therapy session conducted in groups of 20 s as part of the rehabilitation process. The groups were converted to self-help groups after 4 months to generate income for the participants. Results: Factors significantly associated with relapse to alcohol use included severity of alcohol use and craving for alcohol at intake and the age of onset of alcohol drinking. Further there was a statistically significant predictive value in the mean score of alcohol related problems in the community based group (health, social, financial and legal). Conclusion: Identifying factors that are associated with relapse after alcohol dependence treatment is likely to improve the effectiveness of treatment and prevent relapse in persons at risk.

A Photo-Based Environmental History of the Use of Climbing Plants in Central Oxford, UK  [PDF]
Mary J. Thornbush
International Journal of Geosciences (IJG) , 2013, DOI: 10.4236/ijg.2013.47102

This paper examines environmental change associated with climbing plants (ivy/creeper) on several historical buildings in central Oxford using archival photographs. ViewFinder from English Heritage was used to access the photo archives in an advanced search of the area of “Oxford” and in the county of “Oxfordshire”. The study includes a variety of buildings, including colleges, churches, chapels, asylums, inns/hotels, factories, a brewery, pubs, a castle as well as architectural elements, such as doorways, cloisters, gates, and walls. The findings reveal that a majority of photographs denoted ivy-/creeper-clad buildings (in nearly 53% of photographs found mostly in the Taunt collection). The greatest abundance of climbing plants was found in the 1880s followed by the 1900s. A further examination of University colleges is warranted due to the earlier and more frequent appearance of ivy/creeper on these buildings.

Assessment of an Interactive Internet Program to Educate Children Aged 7 - 9 about Science, the Brain and Drugs  [PDF]
Mary P. Metcalf
Creative Education (CE) , 2013, DOI: 10.4236/ce.2013.411097
Abstract: BrainTrain4Kids.com is an interactive science Internet program for children aged 7 - 9 developed with funding from the National Institute on Drug Abuse (NIDA). Based on NIDA’s classroom curriculum, Brain Power!, we adapted and expanded this material to optimize online media. The primary objective of the curriculum is to provide an early foundation for drug abuse prevention efforts by educating elementary school-aged children about the brain and how alcohol, tobacco, and drugs can harm it. Secondary aims include improving knowledge, appreciation for scientific inquiry, and improving/sustaining a positive attitude towards science. The program employs engaging and entertaining elements including Flashbased activities and educational games. Assessment of the program occurred using a two-group, randomized case/control pre/post trial with a crossover design using a convenience sample. Child participants (N = 102) from control and case groups had similar knowledge and attitudes towards science at baseline. At post-intervention, there was a significant increase in knowledge scores for the case group; this increase was retained at the six-week follow-up. Case group attitudes towards science were more positive immediately after post-intervention than at baseline, and at follow up than at baseline. BrainTrain4Kids can be an effective tool for educating children about science and drugs, and has the potential to positively impact attitudes. It can be used as a part of a drug abuse prevention program either in schools or at home.
Self-Pereception of Adults with Intellectual and Developmental Disabilities  [PDF]
Mary Rita Welle
Advances in Applied Sociology (AASoci) , 2014, DOI: 10.4236/aasoci.2014.41005

Adults with intellectual and developmental disabilities (IDD) may view themselves as children; indeed they are often treated in a childlike manner by others. Some may develop a self-perception that they are minors and view children more as peers. Using data from this author’s (Smith, 2006) unpublished dissertation, developmental self-perception is explored by using a subset of questions from the SSKAAT-R instrument (Griffiths & Lunsky, 2003). This study discusses how some adults with IDD self-identify as more closely resembling children. A total of 26 adults with IDD, 17 males and nine females, were involved. With IRB approval, questions that would assess understanding regarding perception of self (along with a follow-up question regarding sexual attraction) were posed. Of the 26 participants, 73% self-identified as more closely resembling adults, whereas the remaining 27% self-identified as more closely resembling children.

Measuring Surface Roughness through the Use of Digital Photography and Image Processing  [PDF]
Mary J. Thornbush
International Journal of Geosciences (IJG) , 2014, DOI: 10.4236/ijg.2014.55050

This paper aims to provide a quantitative method that employs image processing in the assessment of surface roughness based on digital photograph field surveys, as in previous studies employing the outdoor integrated digital photography and image processing (O-IDIP) method. Digital photographs were taken on two different days under contrasting outdoor lighting conditions (overcast versus clear sky). Images were captured mounted on a tripod close up to the surface of a 380-year-old wall located at the University of Oxford Botanic Garden in the City of Oxford, UK. Sampling points were established at regular intervals along the border wall and encompassed sections facing west, north, and east, respectively along the survey. Two photographs were taken with a digital camera at each sampling point, one containing a color chart used to calibrate outdoor lighting conditions across images, which was excluded from the other photographic pair. Histogram-based quantification was performed based on images converted to Lab Color mode. The 10-step calibration procedure presented in this paper required more adjustments of contrast. However, more adjustments were not required under a clear sky. Std Dev L measurements were used to establish categories in a simple 3-point roughness index, namely the surface roughness index (SRI). The results denote that pitting did not affect surface roughness measurements. The study shows that it is possible to use Std Dev L measurements to quantify surface roughness on a comparative basis.

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