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Search Results: 1 - 10 of 219248 matches for " Martha L;Ruiz "
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EFECTO DE LA SUPLEMENTACIóN CON ALANINA Y GLICINA SOBRE LOS CLIVAJES INICIALES DE EMBRIONES BOVINOS PRODUCIDOS IN VITRO
Arias L,Catalina; Ruiz,Tatiana; Olivera A,Martha; Tarazona,Ariel;
Revista MVZ Córdoba , 2007,
Abstract: objective. to determine the effect of supplementation with alanine and glicyne in vitro culture systems on the cleavage rate of bovine embryos under conditions of high (20%) and lower (7%) oxygen tension. materials and methods. the embryos were produced from oocytes matured in vitro in m-199 supplemented with hormones and inseminated with cryopreservated semen; the zygotes were cultivated in cr1-aa medium. at culture moment alanine and glicyne were added (5 mm and 10mm final respectively). four (4) treatments were used (t1: amino acids and low oxygen tension; t2: amino acids and high oxygen tension; t3: non amino acids and low oxygen tension; t4: non amino acids and high oxygen tension). the cleavage was evaluated to 48 hpi (hours post insemination) being evaluated the number of embryos cleavaged on the total of culture embryos and the development stage (non cleavaged, 2, 4, 5-8 cells). the statistical program statistica version 5.0 was used. results. embryos rate of 5-8 cells in the treatment one regarding the other 3 ones were higher (p = 0.007). conclusions. the amino acids alanine, glicyne and in low oxygen tensions are fundamental for the initial cleavages because the proportion of competent embryos increases up to 48 hpi.
EFECTO DEL CO-CULTIVO SOBRE EL DESARROLLO TEMPRANO DE EMBRIONES BOVINOS PRODUCIDOS IN VITRO
L?3pez,Angela; Olivera,Martha; Ruiz,Tatiana; Tarazona,Ariel;
Revista MVZ Córdoba , 2007,
Abstract: objective. to study the effect of co-culture with oviductal cells on the cleavage rate 48 hours post insemination (hpi) of bovine embryos in low oxygen tensions. materials and methods. slaughterhouse ovaries were collected for the extraction of oocytes put on tcm 199 medium supplemented with hormones, fertilized with semen cryo-preserved and co-cultured in cr1aa medium with oviductal cells during 48 hours. total cleavage rate and cleavage per stages of 2-4 cells and 5-8 cells were evaluated. the viability of cells for the co-culture was determined by observation of the ciliary movement and monolayer observation. treatments were t1: oviduct cells + oxygen 20%; t2: oviduct cells + oxygen 7%; t3 and t4 were controls without cells for both oxygen concentrations. results. for cleavage rate there was not significant difference among the four treatments, but there was a tendency to more cleavage for co-cultured embryos with cells and 20% oxygen. conclusions. the use of high oxygen rates (20%) in co-culture systems with oviductal cells intend to improve the cleavage percentages to 48 hpi.
Infecciones nosocomiales en un hospital del tercer nivel: Experiencia de 5 a?os
Frómeta Suárez,Ileana; Izquierdo Cubas,Francisco; López Ruiz,Martha;
Revista Cubana de Medicina , 2008,
Abstract: the system of epidemiological surveillance of nosocomial infections at "hermanos ameijeiras" hospital is applied with a selective approach. the use of health care quality indicators allows to establish immediate control strategies. in this paper, it is described the behavior of these indicators in 4 years of follow-up, stratified by type of invasive procedure. an observational, descriptive and retrospective study was conducted. the rates of incidence and the density of incidence by invasive procedures were calculated. a rate of nosocomial infection of 3.1 % per discharged patient accumulated from 2000 to 2004 was found. it was proved that the sepsis of the lower respiratory tract was the most frequent cause of morbidity with 32 %,the global rate of the surgical wound was 1.4 %, pneumonia associated with mechanical ventilation was 4.2 per 1 000 patient days, whereas uti in patients with vesical catheter was 2.2 per 1 000 patient days. bsi associated with central venous catheter was present at a rate of 2.4 x 1 000 day patients. the rates of incidence of nosocomial infection are comparable with the international patterns and similar to those of the countries with efficient nosocomial infection control programs.
Infecciones nosocomiales en un hospital del tercer nivel: Experiencia de 5 a os Nosocomial infections in a third level hospital: A 5-year experience
Ileana Frómeta Suárez,Francisco Izquierdo Cubas,Martha López Ruiz
Revista Cubana de Medicina , 2008,
Abstract: El sistema de vigilancia epidemiológica de las infecciones nosocomiales en el Hospital "Hermanos Ameijeiras" se realiza con un enfoque selectivo, el empleo de indicadores de calidad asistencial permite establecer estrategias inmediatas de medidas de control. En el presente trabajo se describió el comportamiento de estos indicadores en 4 a os de seguimiento, estratificado por tipo de procedimiento invasivo. Se aplicó un dise o de estudio observacional descriptivo retrospectivo. Se calcularon las tasas de incidencia y la densidad de incidencia por procedimientos invasivos. Se halló una tasa de infección nosocomial acumulada entre el 2000 y el 2004 de 3,1 % por 100 pacientes egresados. Se comprobó que la sepsis del tracto respiratorio bajo fue la causa más frecuente de morbilidad con 32 %, la tasa global de la herida quirúrgica fue de 1,4 %; la neumonía asociada a ventilación mecánica, de 4,2 por 1 000 días pacientes; la STU en pacientes con catéter vesical, de 2,2 por 1 000 días pacientes y la STS asociada a catéter venoso central se presentó en una tasa de 2,4 x 1 000 días pacientes. Las tasas de incidencia de infección nosocomial son comparables con los patrones internacionales, semejantes a las de los países con programas eficientes de control de infecciones nosocomiales. The system of epidemiological surveillance of nosocomial infections at "Hermanos Ameijeiras" Hospital is applied with a selective approach. The use of health care quality indicators allows to establish immediate control strategies. In this paper, it is described the behavior of these indicators in 4 years of follow-up, stratified by type of invasive procedure. An observational, descriptive and retrospective study was conducted. The rates of incidence and the density of incidence by invasive procedures were calculated. A rate of nosocomial infection of 3.1 % per discharged patient accumulated from 2000 to 2004 was found. It was proved that the sepsis of the lower respiratory tract was the most frequent cause of morbidity with 32 %,the global rate of the surgical wound was 1.4 %, pneumonia associated with mechanical ventilation was 4.2 per 1 000 patient days, whereas UTI in patients with vesical catheter was 2.2 per 1 000 patient days. BSI associated with central venous catheter was present at a rate of 2.4 x 1 000 day patients. The rates of incidence of nosocomial infection are comparable with the international patterns and similar to those of the countries with efficient nosocomial infection control programs.
EFECTO DE LA SUPLEMENTACIóN CON ALANINA Y GLICINA SOBRE LOS CLIVAJES INICIALES DE EMBRIONES BOVINOS PRODUCIDOS IN VITRO
Catalina Arias L,Tatiana Ruiz C,Martha Olivera A,Ariel Tarazona M
Revista MVZ Córdoba , 2007,
Abstract: Objetivo. Determinar el efecto de la suplementación con alanina y glicina en el medio de cultivo sobre el porcentaje de clivaje de embriones bovinos, bajo condiciones de alta (20%) y baja (7%) tensión de oxígeno. Materiales y métodos. Los embriones fueron producidos a partir de oocitos madurados in vitro en M-199 suplementado con hormonas e inseminados con semen criopreservado; los cigotos fueron cultivados en medio CR1-AA. Al momento del cultivo se adicionaron alanina y glicina (5 mM y 10mM final respectivamente). Se usaron 4 tratamientos (T1: aminoácidos y baja tensión de oxígeno; T2: aminoácidos y alta tensión de oxígeno; T3: no aminoácidos y baja tensión de oxígeno; T4: no aminoácidos y alta tensión de oxígeno). El clivaje fue evaluado a las 48 hpi (horas post inseminación) evaluándose el número de embriones clivados sobre el total de embriones cultivados y el estadío de desarrollo (no clivados, 2, 4, 5-8 células). Se usó el programa estadístico STATISTICA (versión 5.0). Resultados. El porcentaje de embriones de 5-8 células en el tratamiento 1 respecto a los otros 3 tratamientos fue mayor (p= 0.007). Conclusiones. Los aminoácidos alanina y glicina son fundamentales para los clivajes iniciales y en bajas tensiones de oxígeno se aumenta la proporción de embriones competentes hasta las 48hpi.
EFECTO DEL CO-CULTIVO SOBRE EL DESARROLLO TEMPRANO DE EMBRIONES BOVINOS PRODUCIDOS IN VITRO
Angela López C.,Martha Olivera A.,Tatiana Ruiz C.,Ariel Tarazona M
Revista MVZ Córdoba , 2007,
Abstract: Objetivo. Estudiar el efecto del cocultivo con células oviductales sobre el porcentaje de clivaje 48 horas post inseminación (hpi) de embriones bovinos en bajas tensiones de oxígeno. Materiales y métodos. Se recolectaron ovarios de matadero para la extracción de los ovocitos que fueron puestos en medio TCM 199 suplementado con hormonas, se fertilizaron con semen criópreservado y se cocultivaron en medio CR1aa con células de oviducto durante 48 horas. Se evaluó el porcentaje de clivaje total y el porcentaje de clivaje por estadios de 2-4 células y 5-8 células. La viabilidad de las células para el cocultivo se determinó por observación del movimiento ciliar y observación de monocapa. Los tratamientos fueron T1: células de oviducto + oxígeno 20%; T2: células de oviducto + oxígeno 7%; T3 y T4 fueron controles sin células para ambas concentraciones de oxígeno. Resultados. En cuanto al porcentaje de clivaje no hubo diferencia significativa entre los cuatro tratamientos, pero hubo una tendencia a mayor clivaje para los embriones cocultivados con células y 20% oxígeno. Conclusiones. La utilización de altas tasas de oxígeno (20%) en los sistemas de cococultivo con células oviductales tienden a mejorar los porcentajes de clivaje a las 48 hpi.
Ni sue o ni pesadilla: diversidad y paradojas en el proceso migratorio
Ruiz , Martha Cecilia
Iconos : Revista de Ciencias Sociales , 2002,
Abstract:
Diabetes Mellitus: Aptitud clínica del médico de atención primaria
Gómez-López,Víctor Manuel; García-Ruiz,Martha Elena; Barrientos- Guerrero,Carlos;
Educación Médica , 2006, DOI: 10.4321/S1575-18132006000100008
Abstract: objective: to compare the clinic aptitude of the doctor in the unities of familiar medicine in the care of the diabetes mellitus. material and methods: transversal and comparative pattern. a evaluation previously validated by a group of experts was apply to 78 specialist in familiar medicine who redeem in the first level of attention. the indicators explored in the evaluation are. i recognition of cause of risk. ii recognition of signs and symptoms. iii utilization and interpretation of laboratory studies and consultation studies. iv diagnostic integration. v utilization of terapeutic measures and vi following measures. for the statistical analysis, it was used the kruskall-wallis and chi-square with a level of important of 0.05 results: the points to the explainable for effects of chance were of < 24 in global grade. according to the scale used, the 64 % (ic 95 % de 53 a 70 %) of the results in the grades was situated in the low scale (49-73). in general it didn′t appear significative differences in the results of clinic aptitude by indicator and academic degree. according to objetives of the study, the association between academic degree and the clinic aptitude using the chi-square was 1.331 (p = ns) conclusions: we didn′t find statistically significative difference in the clinic aptitude among the general doctors, specialists in familiar medicine and certificated specialist.
Molecular differentiation of Anopheles (Nyssorhynchus) benarrochi and An. (N.) oswaldoi from Southern Colombia
Ruiz, Freddy;Qui?ones, Martha L;Erazo, Holmes F;Calle, David A;Alzate, Juan F;Linton, Yvonne-Marie;
Memórias do Instituto Oswaldo Cruz , 2005, DOI: 10.1590/S0074-02762005000200008
Abstract: anopheles (nyssorhynchus) benarrochi, an. (n.) oswaldoi, and an. (n.) rangeli are the most common anthropophilic mosquitoes in the southern colombian state of putumayo. adult females are most commonly collected in epidemiological studies, and this stage poses significant problems for correct identification, due to overlapping inter-specific morphological characters. although an. rangeli is easy to identify, the morphological variant of an. benarrochi found in the region and an. oswaldoi are not always easy to separate. herein we provide a rapid molecular method to distinguish these two species in southern colombia. sequence data for the second internal transcribed spacer (its2) region of rdna was generated for link-reared progeny of an. benarrochi and an. oswaldoi, that had been identified using all life stages. its2 sequences were 540 bp in length in an. benarrochi (n = 9) and 531 bp in an. oswaldoi (n = 7). sequences showed no intra-specific variation and ungapped inter-specific sequence divergence was 6.4%. species diagnostic banding patterns were recovered following digestion of the its2 amplicons with the enzyme hae iii as follows: an. benarrochi (365, 137, and 38 bp) and an. oswaldoi (493 and 38 bp). this polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) assay provides rapid, accurate, and inexpensive species diagnosis of adult females. this will benefit future epidemiological studies and, as pcr amplification can be achieved using a single mosquito leg, the remaining specimen can be either retained as a morphological voucher or further used in vector incrimination studies. that an. benarrochi comprises a complex of at least two species across latin america is discussed.
Incrimination of Anopheles (Nyssorhynchus) rangeli and An. (Nys.) oswaldoi as natural vectors of Plasmodium vivax in Southern Colombia
Qui?ones, Martha L;Ruiz, Freddy;Calle, David A;Harbach, Ralph E;Erazo, Holmes F;Linton, Yvonne-Marie;
Memórias do Instituto Oswaldo Cruz , 2006, DOI: 10.1590/S0074-02762006000600007
Abstract: malaria transmission in the southern colombian state of putumayo continues despite the absence of traditional vector species, except for the presence of anopheles darlingi near the southeastern border with the state of amazonas. in order to facilitate malaria vector incrimination in putumayo, 2445 morphologically identified anopheles females were tested for natural infection of plasmodium vivax by elisa. specimens tested included an. apicimacula (n = 2), an. benarrochi b (n = 1617), an. darlingi (n = 29), an. mattogrossensis (n = 7), an. neomaculipalpus (n = 7), an. oswaldoi (n = 362), an. peryassui (n = 1), an. punctimacula (n = 1), an. rangeli (n = 413), and an. triannulatus (n = 6). despite being overwhelmingly the most anthropophilic species in the region and comprising 66.1% of the mosquitoes tested, an. benarrochi b was not shown to be a vector. thirty-five an. rangeli and one an. oswaldoi were naturally infected with p. vivax vk210. sequence data were generated for the nuclear second internal transcriber space region of 31 of these 36 vivax positive mosquitoes (86.1%) to confirm their morphological identification. an. oswaldoi is known to be a species complex in latin america, but its internal taxonomy remains unresolved. herein we show that the an. oswaldoi found in the state of putumayo is genetically similar to specimens from the state of amapá in brazil and from the ocama region in the state of amazonas in venezuela, and that this form harbors natural infections of p. vivax. that an. rangeli and this member of the an. oswaldoi complex are incriminated as malaria vectors in putumayo, is a novel finding of significance for malaria control in southern colombia, and possibly in other areas of latin america.
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