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Meningoencephalitis by Herpesvirus Type 5/ Herpesvírus Bovino Tipo 5 e Meningoencefalite Herpética Bovina
Marlise Pompeo Claus,Alice Fernandes Alfieri,Amauri Alcindo Alfieri
Semina : Ciências Agrárias , 2002,
Abstract: Bovine herpesvirus type 5 is an important pathogen in young and adult cattle and causes meningoencephalitis. The infection, although it causes low morbidity, can cause a lethality rate close to 100%. Diagnosis of the clinical disease is very important due to the similarities with other diseases that affect the cattle central nervous system, especially rabies. This revision aims to present topics related to the etiological agent, the disease, diagnosis and prophylaxis methods of bovine herpetic meningoencephalitis. O herpesvírus bovino tipo 5 é um importante patógeno de bovinos jovens e adultos, e o agente etiológico da meningoencefalite herpética. A infec o, apesar da baixa morbidade, pode ocasionar taxa de letalidade próxima a 100%. Devido às semelhan as com outras enfermidades que comprometem o sistema nervoso central de bovinos, em particular a raiva, o diagnóstico da doen a clínica assume especial importancia. Esta revis o tem por objetivo apresentar tópicos relacionados ao agente etiológico, à doen a e aos métodos de diagnóstico e de profilaxia da meningoencefalite herpética bovina.
Bovine herpesvirus 5 detection by virus isolation in cell culture and multiplex-PCR in central nervous system from cattle with neurological disease in Brazilian herds
Claus, Marlise Pompeo;Alfieri, Alice Fernandes;Médici, Kerlei Cristina;Lunardi, Michele;Alfieri, Amauri Alcindo;
Brazilian Journal of Microbiology , 2007, DOI: 10.1590/S1517-83822007000300019
Abstract: bovine herpesvirus 5 (bohv-5) is an important cause of meningoencephalitis in young and adult cattle. the multiple etiology of neurological disturbances in cattle makes the quick and conclusive diagnosis of bohv-5 infection important for animal and public health, mainly because of herbivore rabies that is endemic in brazilian cattle herds. the objective of this retrospective study was to use a multiplex-polymerase chain reaction (multiplex-pcr) for bohv-5 and bohv-1 glycoprotein c gene detection from stored central nervous system (cns) tissue fragments of cattle with neurological clinical signs. forty-seven frozen cns samples of young and adult cattle from 31 herds in three brazilian geographical regions (south, southeast, and center-west) were evaluated. eighteen (38.3%) of these cns samples were bohv-positive by virus isolation in cell culture. by multiplex-pcr 30 (63.8%) cns samples were bohv-5 positive. all 18 positive samples by virus isolation were confirmed as bohv-5 by the multiplex-pcr, that provided a increase of 25.5% (12/47) in the bohv-5 diagnosis rate. bohv-1 was not detected in any cns sample. this retrospective study demonstrated the wide regional distribution of bohv-5 infection in brazilian cattle herds since positive results were obtained in cns samples of cattle with neurological disease from paraná, s?o paulo, minas gerais, mato grosso, and mato grosso do sul states.
Multiple bovine papillomavirus infections associated with cutaneous papillomatosis in brazilian cattle herds
Claus, Marlise Pompeo;Lunardi, Michele;Alfieri, Amauri Alcindo;Otonel, Rodrigo Alejandro Arellano;Sartori, Daniele;Fungaro, Maria Helena Pelegrinelli;Alfieri, Alice Fernandes;
Brazilian Archives of Biology and Technology , 2009, DOI: 10.1590/S1516-89132009000700013
Abstract: cutaneous papillomatosis is a pathological condition commonly found in cattle and is characterized by the presence of benign proliferative tumors caused by bovine papillomavirus (bpv) infection. while multiple infections with human papillomavirus (hpv) are common in healthy and immunodeficient humans, studies with the aim of identifying mixed infections are still sporadic in veterinary medicine. the aim of this study is to describe the occurrence of multiple bpv infections in cattle affected by cutaneous papillomatosis. fifteen skin warts were collected from at least two diverse anatomical regions of six bovines with papillomatosis belonging to three cattle herds from the paraná state in brazil. the bpv types present in the skin wart samples were determined by a pcr assay performed with the fap primer pair for partial l1 gene amplification followed by direct sequencing or by cloning and sequencing of the inserts. sequence analysis of the obtained amplicons allowed the identification of four characterized bpv types (bpv-1, -2, -6, and -8) and three previously described putative new bpv types (bpv/br-uel3, bpv/br-uel4, and bpv/br-uel5). double infections were identified in four (a, b, d, and e) of the six animals included in this study. in this work, the strategy adopted to evaluate skin warts from diverse anatomical sites of the same animal allowed the identification of multiple infections with two or three different bpv types. the analysis of four animals belonging to a single cattle herd also showed the presence of six different viral types. these results clearly suggest that both multiple papillomaviral infection and a high viral diversity can be as frequent in cattle as in human beings.
Neurological and epidemiological aspects of a BoHV-5 meningoencephalitis outbreak
Lunardi, Michele;Claus, Marlise Pompeo;Lisb?a, Júlio Augusto Naylor;Amude, Alexandre Mendes;Headley, Selwyn Arlington;Alfieri, Alice Fernandes;Alfieri, Amauri Alcindo;
Brazilian Archives of Biology and Technology , 2009, DOI: 10.1590/S1516-89132009000700011
Abstract: bovine herpesvirus 5 is a dna virus that has been associated with meningoencephalitis in young cattle. while its clinical diagnosis is obscured by other major diseases that also produce similar neurological disease in cattle, the use of conventional virological techniques is hampered by the establishment of a lifelong latent infection in the host and the difficulty in differentiating bohv-1 and bohv-5. the aim of the current report is to describe the clinical and epidemiological aspects observed in a natural outbreak of bohv-5 meningoencephalitis in a dairy cattle herd from brazil. in the outbreak, the affected animals consisted of nine calves, which presented three possible forms of the neurological disease, subjectively classified as peracute, acute, and subacute/chronic. in contrast to conventional herpetic meningoencephalitis, characterized mainly by progressive multifocal brain dysfunctions, bohv-5 infection resulted in focal non-progressive caudal brainstem dysfunction (pontomedullary syndrome) in an animal presented with subacute/chronic bohv-5 meningoencephalitis. the evaluation of cns tissue of affected calves through both histological examination and multiplex-pcr was able to confirm bohv-5 infection. additionally, the analysis of csf samples through pcr allowed ante-mortem bohv-5 diagnosis during the outbreak, which enabled the implementation of several measures of control for the disease.
A bovine teat papilloma specimen harboring Deltapapillomavirus (BPV-1) and Xipapillomavirus (BPV-6) representatives
Claus, Marlise Pompeo;Lunardi, Michele;Alfieri, Amauri Alcindo;Otonel, Rodrigo Alejandro Arellano;Ferracin, Lara Munique;Fungaro, Maria Helena Pelegrinelli;Alfieri, Alice Fernandes;
Brazilian Archives of Biology and Technology , 2009, DOI: 10.1590/S1516-89132009000700012
Abstract: the common occurrence of multiple papillomavirus infections has been shown in several studies involving the human host. however, investigations with the aim of identifying mixed papillomavirus infections in cattle have been conducted only recently. in the current work we describe a co-infection with two different bovine papillomavirus (bpv) types that was identified in a bovine teat papilloma. the skin wart was obtained from a cow belonging to a brazilian beef herd. a pcr assay was carried out with the fap primer pair, which amplifies a partial segment of the l1 gene (approximately 478 bp), and the amplicon was submitted to direct sequencing. because nucleotide sequences with satisfactory quality scores were not obtained, the amplicon was cloned and further sequencing, involving ten selected clones, was performed. the sequence analysis of the cloned inserts revealed the presence of two different bpv types. bpv-1 (deltapapillomavirus genus) was detected in six clones, while bpv-6 (xipapillomavirus genus) was detected in four clones. this finding confirms the presence of bpv co-infection associated with cutaneous papillomatosis in cattle.
Phylogenetic position of an uncharacterized Brazilian strain of bovine papillomavirus in the genus Xipapillomavirus based on sequencing of the L1 open reading frame
Lunardi, Michele;Claus, Marlise P.;Alfieri, Amauri A.;Fungaro, Maria Helena P.;Alfieri, Alice F.;
Genetics and Molecular Biology , 2010, DOI: 10.1590/S1415-47572010005000091
Abstract: the use of pcr assays with degenerate primers has suggested the existence of numerous as yet uncharacterized bovine papillomaviruses (bpv). despite the endemic nature of bpv infections, the identification of bpv types in brazilian cattle is still only sporadic. however, in a recent analysis of a partial segment of the l1 gene, we observed notable diversity among the bpv types detected. the aim of this study was to determine the phylogenetic position of the previously identified wild strain bpv/br-uel2 detected in the state of paraná in brazil. since previous analysis of the partial l1 sequence had shown that this strain was most closely related to bpv type 4, genus-specific primers were designed. phylogenetic analysis using complete l1 orf sequences revealed that bpv/br-uel2 was related to bpv types classified in the genus xipapillomavirus and shared the highest l1 nucleotide sequence similarity with bpv type 4 (78%). this finding suggests that bpv/br-uel2 should be classified as a potential new type of bpv in the genus xipapillomavirus.
Bovine papillomavirus type 2 detection in the urinary bladder of cattle with chronic enzootic haematuria
Wosiacki, Sheila R;Claus, Marlise P;Alfieri, Alice F;Alfieri, Amauri A;
Memórias do Instituto Oswaldo Cruz , 2006, DOI: 10.1590/S0074-02762006000600009
Abstract: the bovine papillomavirus type 2 (bpv-2) involvement in the aetiology of chronic enzootic haematuria associated to bracken fern ingestion has been suggested for a long time. however, a few reports have shown the presence of the bpv-2 in urinary bladder tumors of cattle. the aim of this study was to investigate the presence of the bpv-2 infection in the urinary bladder of cattle with chronic enzootic haematuria in brazilian cattle herds. sixty-two urinary bladders were collected from adult cattle in beef herds from the north region of the state of paraná, brazil. according to clinical and pathological finds the specimens were distributed in three groups: the group a was constituted by 22 urinary bladders with macroscopic lesions collected at necropsy of cattle with clinical signs of chronic enzootic haematuria; the group b by 30 urinary bladders with macroscopic lesions collected in a slaughterhouse of cows coming from bracken fern-endemic geographical region; and the group c (control) by 10 urinary bladders without macroscopic lesions collected from asymptomatic cattle in a bracken fern-free geographical region. by a semi-nested polymerase chain reaction (pcr) assay, with an internal control, a fragment of the bpv-2 l1 gene with 386 bp length was amplified in 36 (58%) urinary bladder. the rate of bpv-2 positive urinary bladders was 50% (11/22) for group a, 80% (24/30) for group b, and 10% (1/10) for group c (control). the rate of the positive results found in groups a and b that included urinary bladder samples with macroscopic lesions was 67% (35/52) and the detection of the bpv-2 in both groups was significantly higher (p < 0.05) than in the control group. rflp with rsa i and hae iii enzymes evaluated the specificity of the bpv-2 amplicons. the pcr internal control that amplified a 626 bp fragment of the nd5 gene of the bovine mitochondrial genome was amplified in all analyzed samples and excluded false-negatives or invalid results in the semi-nested pcr. these re
Análise filogenética de papilomavírus bovino associado com les?es cutaneas em rebanhos do Estado do Paraná
Claus, Marlise P.;Vivian, Daniel;Lunardi, Michele;Alfieri, Alice F.;Alfieri, Amauri A.;
Pesquisa Veterinária Brasileira , 2007, DOI: 10.1590/S0100-736X2007000700010
Abstract: bovine papillomavirus (bpv) infection causes hyperplastic lesions in the cutaneous epithelium of cattle. six types of bpv were classified in two sub-groups, being correlated to the anatomical regions of the infection and morphologic characteristics of the lesions. the present study was carried out to identify the types of bpv present in skin warts of cattle from the state of paraná, brazil. the generic primers fap59 and fap64 were used for amplification of a 478 bp fragment of bpv l1 gene in nine cutaneous papilloma samples obtained from six animals in four herds. in all papillomas examined, a product with the expected molecular size was amplified. phylogenetic analysis of the pcr products identified bpv-2 in three samples, bpv-1 in one, and bpv-6 in five papillomas. bpv-6 was detected in cutaneous papillomas of the teat and in other body parts as well. in one animal, from which more than one sample was collected, a concomitant infection by bpv-1 and bpv-2 was identified. the five positive bpv-6 samples showed a nucleotide identity of 100% with the sequence of the reference strain available in genbank. however, differences among bpv-2 and bpv-1 brazilian samples and the respective reference sequences deposited in genbank were observed. molecular comparison of the two bpv-2 strains identified showed the involvement of two viral variants. this study revealed the diversity of bpv types circulating in the state of paraná.
Identification of the recently described new type of bovine papillomavirus (BPV-8) in a Brazilian beef cattle herd
Claus, Marlise P.;Lunardi, Michele;Alfieri, Alice F.;Sartori, Daniele;Fungaro, Maria Helena P.;Alfieri, Amauri A.;
Pesquisa Veterinária Brasileira , 2009, DOI: 10.1590/S0100-736X2009000100003
Abstract: bovine papillomavirus type 8 (bpv-8) was first detected and described in teat warts as well as in healthy teat skin from cattle raised in japan. the entire viral genome was sequenced in 2007. additionally, a variant of bpv-8, bpv-8-eb, was also identified from papillomatous lesions of a european bison in slovakia. in brazil, despite the relatively common occurrence of bpv infections, the identification and determination of viral types present in cattle is still sporadic. the aim of this study is to report the occurrence of the recently described bpv-8 in brazil. the virus was identified in a skin warts obtained from a beef cattle herd located in parana state, southern brazil. the papilloma had a macular, non-verrucous gross aspect and was located on the dorsal thorax of a cow. polymerase chain reaction (pcr) was performed using generic primers for partial amplification of l1 gene. the obtained amplicon (480bp) was cloned and two selected clones were sequenced. the nucleotide sequence was compared to existing papillomaviral genomic sequences, identifying the virus as bpv type 8. this study represents the first report of bpv-8 occurrence in brazil, what suggests its presence among brazilian cattle.
Teorias de gênero ou teorias e gênero? Se e como os estudos de gênero e feministas se transformaram em um campo novo para as ciências
Matos, Marlise;
Revista Estudos Feministas , 2008, DOI: 10.1590/S0104-026X2008000200003
Abstract: this article seeks to define what would be the order of gender studies within the brazilian academic setting today. given three sets of distinct reflections, the article tries to explore gender initially understood as a "theme" and a "concept" to subvert it and postulate gender today as a new scientific field. these three sets of reflections refer to: 1) the place of the current art of gender and feminist studies in brazilian academic reflections; 2) the consequent attempt to explain and delimit the theoretical conceptions in these studies, which includes the objective of going beyond a mere concept, tool or analytic construction, establishing a new field of study in social and human sciences and even a new epistemology in the sciences; and 3) the discussions of the implications and consequences that such an initiative would have on the sciences, in addition to bringing contributions to a feminist epistemology as well as postulating a science with a multicultural and emancipating character.
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