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Search Results: 1 - 10 of 184463 matches for " Marleide Magalh?es de Andrade;Resende "
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Differential gene expression, induced by salicylic acid and Fusarium oxysporum f. sp. lycopersici infection, in tomato
Amaral, Daniel Oliveira Jord?o do;Lima, Marleide Magalhes de Andrade;Resende, Luciane Vilela;Silva, Márcia Vanusa da;
Pesquisa Agropecuária Brasileira , 2008, DOI: 10.1590/S0100-204X2008000800010
Abstract: the objective of this work was to determine the transcript profile of tomato plants (lycopersicon esculentum mill.), during fusarium oxysporum f. sp. lycopersici infection and after foliar application of salicylic acid. the suppression subtractive hybridization (ssh) technique was used to generate a cdna library enriched for transcripts differentially expressed. a total of 307 clones was identified in two subtractive libraries, which allowed the isolation of several defense-related genes that play roles in different mechanisms of plant resistance to phytopathogens. genes with unknown roles were also isolated from the two libraries, which indicates the possibility of identifying new genes not yet reported in studies of stress/defense response. the ssh technique is effective for identification of resistance genes activated by salicylic acid and f. oxysporum f. sp. lycopersici infection. not only the application of this technique enables a cost effective isolation of differentially expressed sequences, but also it allows the identification of novel sequences in tomato from a relative small number of sequences.
Physiological traits for drought phenotyping in cotton = Tra os fisiológicos para fenotipagem de algodoeiro sob seca
Giovani Greigh de Brito,Valdinei Sofiatti,Marleide Magalhes de Andrade Lima,Luiz Paulo de Carvalho
Acta Scientiarum : Agronomy , 2011,
Abstract: The objective of this study was to identify physiological traits that could distinguish between cotton genotypes that were tolerant or sensitive to water deficits. The experiment was conducted in a completely randomized design through a factorial combination to analyze four genotypes (BRS 187 8H and ACALA SJ-4 - water deficittolerant; CNPA 7H and SU-0450/8909 - water deficit sensitive) and two water regimes (watered/always irrigated and stressed/with a water deficit imposed at flowering). Irrigation was suspended for the plants in the water deficit treatment groups when their first flowersappeared. Leaf water potential ( pd) was monitored until the plants reached -3.0 MPa predawn, at which point leaf samples were collected for analysis. The plants were reirrigated and monitored for a recovery to 50% of leaf water potential. The maximum photochemical efficiency (Fv/Fm), chlorophyll content (SPAD index), relative watercontent (RWC), disruption of the cell membrane via membrane leakage, carbon isotope composition ( 13C), seed cotton yield and fiber quality were evaluated. The trends in membrane leakage and carbon isotope composition were different between the tolerant and sensitive genotypes under a water deficit, which makes these physiological traits suitable for screening for tolerance to water deficits in cotton. Objetivou-se identificar variaveis fisiologicas para distinguir genotipos de algodoeiro tolerantes e sensiveis ao deficit hidrico. O experimento foi conduzido no delineamento inteiramente casualizado emarranjo fatorial, sendo testados quatro genotipos (BRS 187 8H e ACALA SJ-4 . tolerante ao deficit hidrico; CNPA 7H e SU-0450/8909 - sensiveis ao deficit hidrico) e dois regimes hidricos (controle . sempre irrigado e com deficit hidrico imposto na emissao da primeira flor. Na emissao da primeira flor, a irrigacao foi suspensa para o grupo a ser submetido ao deficit hidrico. O potencial hidrico foliar foi monitorado na antemanha ate que as plantas dos cultivares em estudo atingissem -3,0 MPa, ponto no qual coletaram-se amostras foliares para analises fisiologicas. Apos, irrigou-se as plantas ate a obtencao de valor superior a 50% do potencial hidrico foliar (-1,50 MPa). Avaliaram-se a eficiencia fotoquimica maxima (Fv/Fm), o conteudo de clorofila via indice SPAD, o conteudo relativo de agua (CRA), o extravasamento de eletrolitos, a composicao isotopica do carbono ( 13C), o rendimento de algodao em caroco por planta e a qualidade da fibra. A disruptura de membrana via extravasamento de eletrolitos e a 13C apresentaram-se como potenciais indica
Qualidade fisiológica de sementes de algodoeiro submetidas ao condicionamento mátrico e osmótico
Queiroga, Vicente de Paula;Bruno, Riselane de Lucena Alcantara;Lima, Marleide Magalhes de Andrade;Santos, José Wellingthon dos;
Revista Ceres , 2011, DOI: 10.1590/S0034-737X2011000100009
Abstract: the objective of this work was to evaluate the effect of matrix and osmotic conditioning on the physiological quality of seeds of herbaceous cotton. seeds of the chemically delinted cotton cultivar panton were used at the ratios of 100 g of seeds per 1000 g of solid matrix (algalita). the treatments consisted of five levels of water used for wetting the matrix (0.10, 0.25, 0.50, 0.75 and 1.00 ml) during six conditioning periods (0, 1, 4, 8, 16 and 32 days). two assays were carried out for the osmotic conditioning: seeds treated with osmotic solutions of polyethylene glycol (peg) 6000 (-0,25 mpa) and potassium nitrate (0,3 m or -0.72 mpa) at seven times of osmotic conditioning (0, 12, 24, 36, 48, 72 and 96 h) at 20 ° c. the variables analyzed were percentage of germination and length of seedlings. the experiment was arranged in a complete randomized design with four replications. there was increase in the vigor of seeds conditioned with different levels of moisture in the solid matrix compared with the control. the ability to reinvigorate of the treatment with 0.50 ml water was confirmed with the increased time of seed exposure to the solid matrix; the osmotic conditioning with peg 6000 increased seed vigor, but did not promote germination, and the osmotic conditioning with potassium nitrate reduced germination, but increased seed vigor in the first 24 hours of hydration - of seeds disinfested with sodium hypochlorite - and up to 48 hours for seeds treated with n-[(trichloromethyl) tio]-4-cyclohexene-1 ,2-dicarboximide (concentration of 750 g / kg).
Qualidade de sementes de algod o submetidas aos processos de encapsulamento com e sem corante. = Quality of cotton seeds submitted to encapsulation process with and without dye.
Vicente de Paula Queiroga,Jose Maria Durán,Marleide Magalhes de Andrade Lima,Diego Antonio Nóbrega Queiroga
Agro@mbiente On-line , 2011,
Abstract: Objetivou-se estudar a qualidade fisiológica de sementes de algod o quando submetidas aos processos de encapsulamento com e sem corante em compara o com as sementes apenas tratadas com fungicidas (carboxin e thiran 200 Sc) e inseticida (pirimiphos methyl) - testemunha. A betoneira grande (capacidade de 40 L) recebeu sementes deslintadas +tratadas para serem submetidas ao processo de encapsula o (coating e finishing) com e sem corante, além de uma testemunha n o encapsulada, estabelecendo-se os seguintes tratamentos: 1- sementes deslintadas e tratadas com fungicidas (carboxin e thiran 200 Sc) e inseticidas (pirimiphos methyl) (testemunha); 2- sementes deslintadas, tratadas e encapsuladas (coating e finishing) sem corante; e 3- sementes deslintadas, tratadas e encapsuladas com corante. Foi adotado o delineamento inteiramente casualizado com três tratamentos e quatro repeti es. As variáveis analisadas foram percentagem de germina o, comprimento de plantulas e massa de 100 sementes. Observou-se que o processo de recobrimento de sementes de algod o deslintadas,tratadas com fungicidas e inseticida e encapsuladas n o ocasiona redu o na qualidade fisiológica das sementes, e o uso de corante em sementes encapsuladas n o altera a sua qualidade. = This study evaluated the physiological quality of cotton seeds when subjected to encapsulation with and without dye compared with seeds treated only with fungicides (carboxin and thiran 200 Sc) and insecticide (pirimiphos methyl) - control. The larger mixer (capacity 40 L) received delinted seeds + treated to undergo the process of encapsulation (coating and finishing) with and without dye, and non-encapsulated served as control. The following treatments were established: 1 - delinted seeds and treated with fungicides (carboxin and 200 thiran Sc) and insecticides (pirimiphos methyl) (control), 2 - delinted seeds, processed and encapsulated (coating and finishing) without dye, and 3 - delinted seeds, processed and encapsulated with dye. It was adopted a randomized delineation with three replications. The variables were germination percentage, seedling length and mass of 100 seeds. It was observed that the coating process of cotton delinted seeds, treated with fungicides and insecticides and encapsulated does not cause reduction in seed physiological quality, and the use of dye on encapsulated in seeds does not change its quality.
Physiological traits for drought phenotyping in cotton
Brito, Giovani Greigh de;Sofiatti, Valdinei;Lima, Marleide Magalhes de Andrade;Carvalho, Luiz Paulo de;Silva Filho, Jo?o Luiz da;
Acta Scientiarum. Agronomy , 2011, DOI: 10.4025/actasciagron.v33i1.9839
Abstract: the objective of this study was to identify physiological traits that could distinguish between cotton genotypes that were tolerant or sensitive to water deficits. the experiment was conducted in a completely randomized design through a factorial combination to analyze four genotypes (brs 187 8h and acala sj-4 - water deficit tolerant; cnpa 7h and su-0450/8909 - water deficit sensitive) and two water regimes (watered/always irrigated and stressed/with a water deficit imposed at flowering). irrigation was suspended for the plants in the water deficit treatment groups when their first flowers appeared. leaf water potential (ψpd) was monitored until the plants reached -3.0 mpa predawn, at which point leaf samples were collected for analysis. the plants were re-irrigated and monitored for a recovery to 50% of leaf water potential. the maximum photochemical efficiency (fv/fm), chlorophyll content (spad index), relative water content (rwc), disruption of the cell membrane via membrane leakage, carbon isotope composition (δ13c), seed cotton yield and fiber quality were evaluated. the trends in membrane leakage and carbon isotope composition were different between the tolerant and sensitive genotypes under a water deficit, which makes these physiological traits suitable for screening for tolerance to water deficits in cotton.
Application of different fertilizers in substrate for Kalanchoe luciae Raym.-Hamet cultivation
Lessa, Marília Andrade;Paiva, Patrícia Duarte de Oliveira;Alves, Camila Magalhes Lameiras;Resende, Maria Leandra;
Ciência e Agrotecnologia , 2009, DOI: 10.1590/S1413-70542009000400002
Abstract: succulents present great commercial importance, but information on the cultivation of these plants are not enough, especially considering the use of fertilizers during the growth phase. the objective of this study was to evaluate the effect of different fertilizers applied to the substrate in the cultivation of kalanchoe luciae raym.-hamet. cuttings measuring 6 cm in height and 12 cm in diameter were cultivated in 1.5 l pots containing the substrate (1 soil: 1 sand: 1 vermiculite). in this experiment, the effect of the fertilization based on npk 10:10:10 was evaluated with and without the addition of a fertilizer mixture composed of 5 g bone flour, 5 g dolomite limestone, and 50 g ground charcoal, this proportion that was used for each 1.5 l substrate. after 60 days, an increase in the number of leaves, which was proportional to the increase in the doses of npk applied, was verified. even though the plants presented higher number of leaves (18.34) and higher height (18.73 cm) for the substrate with 1.5 g l-1 of npk 10:10:10, plants did not reach the compact aspect suggested by some producers. the addition of fertilizer mixture presented no effect on the leaves number and neither to the plant height, however, it was observed that the plants that received the fertilizer mixture presented the fifth leaf with bigger diameter. none of the treatments favored the formation of one of the most important characteristics of this species, the reddish color on the borders of the leaves.
Germination of cotton cultivar seeds under water stress induced by polyethyleneglycol-6000
Meneses, Carlos Henrique Salvino Gadelha;Bruno, Riselane de Lucena Alcantara;Fernandes, Pedro Dantas;Pereira, Walter Esfrain;Lima, Leonardo Henrique Guedes de Morais;Lima, Marleide Magalhes de Andrade;Vidal, Márcia Soares;
Scientia Agricola , 2011, DOI: 10.1590/S0103-90162011000200001
Abstract: the physiological quality of cotton cultivar seeds (gossypium hirsutum var. latifolium l.) was evaluated in laboratory by the simulation of water potentials with polyethyleneglycol-6000 (0.0; -0.2; -0.4; -0.6; -0.8 and -1.0 mpa), at 25oc using germitest paper as substrate. a completely randomized design in a 4 × 6 factorial scheme with four replications of 50 seeds each was used. the studied variables were: germination percentage, first count of germination, germination velocity index, accelerated aging in water, electrical conductivity, humidity, vigor classification, radicle length and radicle/shoot length ratio. the effect of water stress on seed viability and on plantlet vigor was severe at potentials below -0.4 mpa. the 'cnpa 187 8h' cultivar was the least sensitive to the tested osmotic potentials, both in terms of germination and of vigor. the 'brs-201' cultivar was mostly affected by the viability and vigor tests under water deficit conditions. differential viability and vigor between cultivars were observed under the water stress levels.
Proposta para um resgate historiográfico: as fontes do SESP/FSESP no estudo das campanhas de imuniza??o no Brasil
Andrade, Márcio Magalhes de;
História, Ciências, Saúde-Manguinhos , 2003, DOI: 10.1590/S0104-59702003000500021
Abstract: the article points to some ways of approaching the history of public health in brazil through use of documentation from the country's special public health service collection (sesp/fsesp), a valuable source that can contribute to achieving a broader understanding of the immunization campaigns undertaken in the latter half of the 20th century. in conjunction with documents from the fundo cláudio amaral and interviews with individuals involved in immunization campaigns (all stored at casa de oswaldo cruz's department of archives and documentation), the sesp documentation helps paint an elucidating picture of many of the sanitation activities carried out in brazil over the course of some six decades.
Proposta para um resgate historiográfico: as fontes do SESP/FSESP no estudo das campanhas de imuniza o no Brasil
Andrade Márcio Magalhes de
História, Ciências, Saúde-Manguinhos , 2003,
Abstract: Além de indicar algumas possibilidades de abordagem da história da saúde pública no Brasil a partir da documenta o pertencente à Cole o SESP/FSESP, este texto busca colocar em destaque, ainda que de maneira sucinta, o valor de tais fontes para a compreens o mais ampla das campanhas de imuniza o empreendidas na segunda metade do século XX. Juntamente com os documentos pertencentes ao Fundo Cláudio Amaral e as entrevistas de personagens envolvidos em campanhas de imuniza o no Brasil - todos sob a guarda no Departamento de Arquivo e Documenta o da Casa de Oswaldo Cruz -, a documenta o legada pelo Servi o Especial de Saúde Pública (SESP) ajudará a compor um quadro esclarecedor de muitas das atividades sanitárias desenvolvidas no país ao longo de aproximadamente seis décadas.
Synthesis and Characterization of Poly(D,L-Lactide-co-Glycolide) Copolymer  [PDF]
Cynthia D’Avila Carvalho Erbetta, Ricardo José Alves, Jarbas Magalhes Resende, Roberto Fernando de Souza Freitas, Ricardo Geraldo de Sousa
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2012, DOI: 10.4236/jbnb.2012.32027
Abstract: The copolymer poly(D,L-lactide-co-glycolide) is one of the most interesting polymers for medical applications. This interest is justified by the fact that it is bioreabsorbable, biocompatible and non-toxic, while its degradation kinetics can be modified by the copolymerization ratio of the monomers. In this study, copolymers were synthesised at 175?C by opening the rings of the cyclic dimers of the D,L-lactide and glycolide monomers in the presence of stannous octoate initiator and lauryl alcohol co-initiator. The application of vacuum to the reaction medium, coupled with adequate stirring, is essential for obtaining good results. The following analytical techniques were used to characterise the synthesised copolymers: Differential Scanning Calorimetry (DSC), Thermogravimetry (TG), Nuclear Magnetic Resonance Spectroscopy (NMR) and Fourier Transform Infrared Spectroscopy (FTIR). Both the input monomers and the reaction products were analysed. Important characteristics, such as melting temperature, glass transition temperature, thermal stability, chemical composition and the ratio of the monomers in the synthesised copolymer, were obtained from these analyses. These results helped to infer the absence of residual monomers in the synthesised copolymers.
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