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Search Results: 1 - 10 of 23187 matches for " Marilda Souza Goncalves "
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Sickle cell disease: Only one road, but different pathways for inflammation  [PDF]
Wendell Vilas-Boas, Bruno Ant?nio Veloso Cerqueira, Thassila Nogueira Pitanga, Magda Oliveira Seixas, Joelma Menezes, Cyntia Cajado de Souza, Elisangela Vitória Adorno, Marilda Souza Goncalves
Advances in Bioscience and Biotechnology (ABB) , 2012, DOI: 10.4236/abb.2012.324071
Abstract: Sickle cell disease (SCD) is a genetic disorder characterized by a chronic inflammatory process, and new biomarkers have been studied as promising molecules for understanding the inflammation in its pathophysiology. The hemolysis and the release of molecules associated to the hemoglobin (Hb) catabolism, such as free Hb, iron, and heme, generating a oxidant environment with production of reactive oxygen and nitrogen species. The immune system plays a very important role in the inflammation, with cells secreting pro-inflammatory cytokines and chemokines. There is also a nitric oxide (NO) resistance state, with an impaired NO bioactivity, leading to a vascular dys-function; activation of platelet, leukocytes, erythrocytes, and endothelial cells, with expression of adhesion molecules and its ligands, and several receptors, that altogether participate at inflammatory process. During inflammation, there is an increase of dendritic cells (DCs) expresse toll like receptors (TLR), but the role of DCs and TLR in SCD pathogenesis is unclear. Also, there are molecules contributing for enhance the endothelium dysfunction, such as homocysteine that has been associated with vascular complications in the pathology of other diseases and it may contribute to the vascular complications presented by SCD patients. Circulating microparticules (MPs) levels are augmented in several diseases and have been described in SCD, where cells membrane compounds are associated to cell’s thrombotic and coagulation state, such as tissue factor and phosphatidylserine (PS), which may contribute to endothelial dysfunction. The knowledge of all these biomarkers may contribute to new therapeutic approach discover, improveing SCD patient life quality.
Cytokine profiles in sickle cell anemia: Pathways to be unraveled  [PDF]
Thassila Nogueira Pitanga, Wendell Vilas-Boas, Bruno Ant?nio Veloso Cerqueira, Magda Oliveira Seixas, Cynara Gomes Barbosa, Elisangela Vitória Adorno, Marilda Souza Goncalves
Advances in Bioscience and Biotechnology (ABB) , 2013, DOI: 10.4236/abb.2013.47A1002
Abstract: Sickle cell anemia (SCA) is a genetically inherited hemolytic disorder characterized by chronic inflammation. Cytokine expression affects the pivotal pathways that contribute to disease pathogenesis, but the mechanisms involved are not well understood. SCA is associated with a proinflammatory state, and an enhanced inflammatory response occurs during vasoocclusive crisis. The immune system thus plays an important role in this inflammatory condition, with several cell types secreting pro-inflammatory cytokines that contribute to the occurrence of common cyclical events in SCA patients, such as hemolysis, vascular occlusion and inflammation. Studies of these cytokines and chemokines in SCA patients have clarified the mechanisms that underlie this disease and highlighted the need for a better understanding of cytokine participation in SCA pathophysiology.
The Host Genetic Diversity in Malaria Infection
Vitor R. R. de Mendonça,Marilda Souza Goncalves,Manoel Barral-Netto
Journal of Tropical Medicine , 2012, DOI: 10.1155/2012/940616
The Host Genetic Diversity in Malaria Infection
Vitor R. R. de Mendon?a,Marilda Souza Goncalves,Manoel Barral-Netto
Journal of Tropical Medicine , 2012, DOI: 10.1155/2012/940616
Abstract: Populations exposed to Plasmodium infection develop genetic mechanisms of protection against severe disease. The clinical manifestation of malaria results primarily from the lysis of infected erythrocytes and subsequent immune and inflammatory responses. Herein, we review the genetic alterations associated with erythrocytes or mediators of the immune system, which might influence malaria outcome. Moreover, polymorphisms in genes related to molecules involved in mechanisms of cytoadherence and their influence on malaria pathology are also discussed. The results of some studies have suggested that the combinatorial effects of a set of genetic factors in the erythrocyte-immunology pathway might be relevant to host resistance or susceptibility against Plasmodium infection. However, these results must be interpreted with caution because of the differences observed in the functionality and frequency of polymorphisms within different populations. With the recent advances in molecular biology techniques, more robust studies with reliable data have been reported, and the results of these studies have identified individual genetic factors for consideration in preventing severe disease and the individual response to treatment. 1. Introduction Malaria is one of the most important and prevalent infectious diseases in the world. The World Health Organization (WHO) estimated 225 million malaria cases worldwide with 781,000 deaths due to Plasmodium infection per year [1]. Four species of Plasmodium (P. falciparum, P. vivax, P. malariae, and P. ovale) are responsible for almost all human infections [2]. Malaria has been associated to gene selective pressure in the human genome, and it has been associated as an evolutionary force of some genetic diseases, such as sickle cell disease (SCD), thalassemia, glucose-6-phosphate dehydrogenase (G6PD) deficiency, and other red blood cell (RBC) genetic anemia with Mendelian inheritance. Haldane (1949) suggested a “balanced polymorphism” where the hemoglobin S (HbS) homozygote disadvantage is recompensed through the resistance of the heterozygote (HbAS) in regions where malaria is endemic [3]. Reports associating several genetic disorders with malaria susceptibility or resistance are on the rise, and studies of heritability indicate that approximately 25% of the risk for severe malaria progression is determined through human genetic factors [4]. Genetic epidemiology may help in pointing out major molecular pathways of some infectious diseases, such as malaria, which involve a robust immune and inflammatory response and the
Methylenetetrahydrofolate reductase polymorphisms in myeloid leukemia patients from Northeastern Brazil
Barbosa, Cynara Gomes;Souza, Claudio Lima;Moura Neto, José Pereira de;Arruda, Maria da Glória Bomfim;Barreto, José Henrique;Reis, Mitermayer Galv?o;Goncalves, Marilda Souza;
Genetics and Molecular Biology , 2008, DOI: 10.1590/S1415-47572008000100005
Abstract: methylenetetrahydrofolate reductase (mthfr: ec polymorphisms are associated to acute lymphoid leukemia in different populations. we used the polymerase chain reaction and the restriction fragment length polymorphism method (pcr-rflp) to investigate mthfr c677t and a1298c polymorphism frequencies in 67 patients with chronic myeloid leukemia (cml), 27 with acute myeloid leukemia fab subtype m3 (aml-m3) and 100 apparently healthy controls. the mthfr mutant allele frequencies were as follows: cml = 17.2% for c677t, 21.6% for a1298c; aml-m3 = 22.2% for c677t, 24.1% for a1298c; and controls = 20.5% for c677t, 21% for a1298c. taken together, our results provide evidence that mthfr polymorphisms have no influence on the development of cml or aml-m3.
A dynamical model for competing opinions
S. R. Souza,S. Goncalves
Computer Science , 2012, DOI: 10.1103/PhysRevE.85.056103
Abstract: We propose an opinion model based on agents located at the vertices of a regular lattice. Each agent has an independent opinion (among an arbitrary, but fixed, number of choices) and its own degree of conviction. The latter changes every time it interacts with another agent who has a different opinion. The dynamics leads to size distributions of clusters (made up of agents which have the same opinion and are located at contiguous spatial positions) which follow a power law, as long as the range of the interaction between the agents is not too short, i.e. the system self-organizes into a critical state. Short range interactions lead to an exponential cut off in the size distribution and to spatial correlations which cause agents which have the same opinion to be closely grouped. When the diversity of opinions is restricted to two, non-consensus dynamic is observed, with unequal population fractions, whereas consensus is reached if the agents are also allowed to interact with those which are located far from them.
Evaluation of Antimicrobial Susceptibility by Staphylococcus aureus Isolated from the Nutrition Service of a Teaching Hospital  [PDF]
Marilda Moreira da Silva, Giovana Nicolete, Barbara Dayene Lopes, Carolina Hiromi Morita, Joyce Marinho de Souza, Lizziane Kretli Winkelstroter, Marcus Vinícius Pimenta Rodrigues
Advances in Microbiology (AiM) , 2018, DOI: 10.4236/aim.2018.84018
Abstract: The bacteria Staphylococcus aureus is found in the nasal passages, mouth and skin of the human population and stands out among foodborne diseases. Some causes of foodborne illness are related with food, handlers, and utensils contamination in a hospital’s nutrition service. The aim of this study was to investigate the presence of S. aureus in food handlers, equipment, counter-tops and utensils of the nutrition service of a teaching hospital. Samples of the environment, hands and nasal mucosa of employees of a nutrition service were collected with two sterile swabs, in 2016, March and June. A total of 134 samples were obtained, which were submitted to characterization tests Biochemistry and morphotinorial (gram staining, catalase and coagulase tests in tube), phenotypic evaluation by drug diffusion technique and D-test approach, and the biofilm phenotype characterization by culture on Congo Red Agar. Results showed high rates of S. aureus colonization, mainly in the kitchen, high frequency of antimicrobial resistance, especially erythromycin and the presence of multi-resistant microorganisms. Large number of positive samples was also found for biofilm production, with totality for the samples of handlers. We highlight the relevance of the data in virtue of the serious consequences and risks that can be triggered in the hospital environment.
Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil
Santos, Fred Luciano Neves;Gon?alves, Marilda de Souza;Soares, Neci Matos;
Brazilian Journal of Infectious Diseases , 2011, DOI: 10.1590/S1413-86702011000200005
Abstract: amoebiasis is an infection caused by entamoeba histolytica and is a potential health risk in countries in which health barriers are inappropriate. since the discovery of entamoeba dispar, the prevalence of amoebiasis has been modified. objective: this study has standardized the pcr technique applied for the diagnosis of different species of the e. histolytica/e. dispar complex and has evaluated the prevalence of infection among patients attending private and public clinical laboratories in salvador city, bahia state, brazil. results: analysis of 52,704 stool samples by microscopic examination demonstrated that 1,788 (3.4%) were positive for the e. histolytica/e. dispar complex and infection occurred more often in samples originated from public clinical laboratories (5.0%) than those that came from private laboratories (3.2%). pcr performed in approximately 15% (262) e. histolytica/e. dispar complex positive samples, randomly chosen, amplified 227 samples (86.6%), all of them positive for e. dispar. the non-amplified 35 samples (13.4%) were also negative for e. histolytica-specific galactose adhesin. moreover, to exclude a probable infection caused by e. hartmanni, morphometric analysis demonstrated that non-amplified samples had cyst sizes comparable to e. histolytica/e. dispar (>10 μm). conclusion: the absence of amplification of these samples indicates the presence of pcr inhibitors in the stool samples or the presence of dna from entamoeba species other than e. dispar, e. histolytica or e. hartmanni.
Clinical, hematological, and molecular characterization of sickle cell anemia pediatric patients from two different cities in Brazil
Lyra Isa Menezes,Gon?alves Marilda Souza,Braga Joseffina A. Pellegrinei,Gesteira Maria de Fátima
Cadernos de Saúde Pública , 2005,
Abstract: This study focused on clinical, hematological, and molecular aspects of sickle cell anemia pediatric patients from two different cites in Brazil. Seventy-one patients from S o Paulo and Salvador, aged 3 to 18 years, were evaluated. Hematological analyses, betaS globin gene haplotypes, and alpha2 3.7kb-thalassemia were performed. Numbers of hospitalizations due to vaso-occlusive crises, infections, stroke, and cholelithiasis were investigated. S o Paulo had more hospitalizations from vaso-occlusion, cholelithiasis, and stroke than Salvador. The Ben/CAR genotype predominated in both cities. alpha2 3.7kb-thalassemia had a frequency of 28.2% in Salvador, mostly with Ben/CAR genotype (45.0%), while S o Paulo had 22.5% with similar frequencies of the Ben/ CAR and CAR/CAR genotypes. Sickle cell anemia patients from S o Paulo also had more episodes of stroke, which was observed among CAR/CAR, atypical, and BEN/CAR haplotypes. In Salvador stroke was only observed in the Ben/CAR genotype. Cholelithiasis had similar frequencies in the two cities. These data suggest a milder phenotype among patients in Salvador, possibly due to genetic, environmental, and socioeconomic factors. Further studies are needed to elucidate modulating factors and phenotype association.
Estudo morfológico comparativo de ovos e ninfas de Triatoma maculata (Erichson, 1848) e Triatoma pseudomaculata Corrêa & Espínola, 1964 (Hemiptera, Reduviidae, Triatominae)
Goncalves, Teresa Cristina Monte;Jurberg, José;Costa, Jane Margaret;Souza, Wanderley de;
Memórias do Instituto Oswaldo Cruz , 1985, DOI: 10.1590/S0074-02761985000300002
Abstract: as an aid in the identification of vectors of chagas' disease we have studied the structure of the eggs and morphology of nymphs by optical microscopy (om) and scanning electron microscopy (sem). the eggs as seen by om show the exochorion of the body doesn't have defined areas. in sem the exochorion of the cap appears as upholstered polygonal areas with small irregular grooves and perforations spread at random in both species. the exochorion of the body in t. maculata is upholstered with more numerous perforations on the edge visualizing the rim of the shell, spermatic groove, pseudomicropyles and micropyles; in t. pseudomaculata there are flat areas with numerous perforations. the nymphs show differences cencerning the stridulatory groove and rostrum. the stridulatory groove when seen by om shows differences in nymphs of 1st, 2nd and 3rd intars, while 4th and 5th are similar; in sem the differences are clear. in om the rostrum shows typical pilosity to each species from 3rd instar. in t. maculata the setae are short and scattered on the 1st and 2nd segments and are long and numerous on the 3rd. in t. pseudomaculata the setae are similar but are short on the 3rd segment.
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